| 引用本文: | 高瞻,陈强,黎中宝,李文静,黄永春,徐安乐,杨敏.饲料添加发酵浒苔对花鲈(Lateolabrax japonicus) 生长性能、消化酶活性和免疫的影响.海洋与湖沼,2015,46(6):1549-1556. |
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| 饲料添加发酵浒苔对花鲈(Lateolabrax japonicus) 生长性能、消化酶活性和免疫的影响 |
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高瞻1,2, 陈强1,2, 黎中宝1,2, 李文静1,2, 黄永春1,2, 徐安乐1,2, 杨敏1,2
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1.集美大学水产学院 厦门 361021;2.福建省海洋渔业资源与生态环境重点实验室 厦门 361021
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| 摘要: |
| 采用单因子试验设计方法, 配制发酵浒苔添加比例分别为0%、1%、2%、3%、4%、5%的 6 种饲料, 探讨发酵浒苔对花鲈生长、体成分、胃、肠消化酶活性及血清、肝脏免疫的影响的研究。 将其饲喂初始平均体重为(6.40±0.14)g 的花鲈28d, 每组饲料设3 个重复, 每个重复投喂30 尾花鲈。 结果表明: (1) 随着发酵浒苔添加水平的升高各组花鲈增重率和特定生长率呈现先升高后降低的趋 势, 且差异性显著(P<0.05), 以2%组添加量最优。相比于对照组, 各添加组饲料系数均有下降。添加 发酵浒苔可适当降低鱼体中的水分, 提高灰分含量但并不显著(P>0.05).同时各添加组的粗脂肪和粗 蛋白含量均高于对照组, 各添加组之间并无显著性差异(P>0.05).(2) 在添加组中, 花鲈淀粉酶、脂 肪酶活性以及胰蛋白酶、胃蛋白酶活性均得到了显著提高(P<0.05).添加水平在2%-3%效果最好。 (3) 添加发酵浒苔可以显著提高血清超氧化物歧化酶、溶菌酶的活性(P<0.05), 但对碱性磷酸酶的活 性差异不显著(P>0.05).本试验中肝脏超氧化物歧化酶、溶菌酶和碱性磷酸酶的活性均得到提高, 在 2%-3%组为最佳。 |
| 关键词: 花鲈 发酵浒苔 生长性能 消化酶活性 免疫力 |
| DOI：10.11693/hyhz20150200057 |
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| 基金项目:福建省科技计划项目资助, 2015N0010 号; 厦门市科技项目资助; 3502Z20143017 号;福建省自然科学基金项目资助, 2013J01139 号; 福建省海洋渔业资源与生态环境重点实验室开放基金资助项目, 201504030001 号。 |
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| EFFECTS OF FERMENTED ENTEROMORPHA PROLIFERA ON GROWTH PERFORMANCE, DIGESTIVE ENZYME ACTIVITIES AND SERUM NON-SPECIFIC IMMUNITY OF LATEOLABRAX JAPONICUS |
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GAO Zhan1,2, CHEN Qiang1,2, LI Zhong-Bao1,2, LI Wen-Jing1,2, HUANG Yong-Chun1,2, XU An-Le1,2, YANG Min1,2
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1.Fisheries College, Jimei University, Xiamen 361021, China;2.Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China
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| Abstract: |
| To evaluate the effect of fermentation Enteromorpha prolifera on growth performance, digestive enzyme activities and serum non-specific immunity 6 diets adding 0%, 1%, 2%, 3%, 4%, 5% fermentation E. prolifera were respectively fed to Lateolabrax japonicus (6.40±0.14)g for 28 days. Each diet was randomly fed to one group with 3 replicates of 30 individuals and single-factor experimental design was employed in this study. The results showed that with increase of fermentation E. prolifera level, L. japonicus weight gain ratio and the specific growth ratio in each group showed a trend of first rising then falling, and got significant difference finally (P<0.05). While the concentration of multivitamin was 2%, weight gain ratio and the specific growth ratio had reached the highest peak. E. prolifera significantly reduced the feed conversion ratio while the crude protein and the crude lipid increased significantly but no significant differences among each adding group (P>0.05). The activities of intestinal trypsin, amylase and lipase of multivitamin added groups increased significantly with the control group (P<0.05). For intestinal trypsin, amylase and lipase, the activities reached the maximum, when fish fed with diet adding 2%-3% fermentation, meanwhile, adding fermentation E. prolifera. Enhanced the activities of serum superoxide dismutase and lysozyme significantly, but no significant differences were found in the activities of alkaline phosphatase between trials (P>0.05). Also, the activities of liver superoxide dismutase, lysozyme and alkaline phosphatase increased significantly. Based on results, it was suggested that the optimization 2% to 3% of fermentation E. prolifera as additives. |
| Key words: Lateolabrax japonicus fermented Enteromorpha prolifera growth performance activities of digestive enzymes immune response |
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