| 摘要: |
| 采用PCR 技术扩增了栉孔扇贝( Chlamys farreri) 线粒体DNA 的16S rRNA 基因片段, PCR 产物经T 载体连接之后进行了克隆、测序, 得到634bp 的核苷酸序列; 分析了该片段的大连、烟台、青岛、朝鲜4 个自然群体31 个个体的核苷酸序列多态性, 共检测到26 个多态性核苷酸位点、18 种单倍型。结果表明, 4 个群体中以朝鲜群体遗传多样性最高, 其次为烟台、青岛群体, 大连群体最低; 不同自然分布区的栉孔扇贝未出现明显的遗传分化。 |
| 关键词: 栉孔扇贝, 线粒体DNA, 16S rRNA基因, DNA序列测定 |
| DOI： |
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| 基金项目:国家重点基础研究发展规划项目“973”资助, G1999012008 号; 国家自然科学基金资助项目, 39600113 号 |
附件 |
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| SEQUENCE POLYMORPHISM OF MITOCHONDRIAL 16S rRNA GENE FRAGMENT IN SCALLOP CHLAMYS FARRERI |
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LIU Ya-Jun1, YU Zi-Niu1, JIANG Yan-Yan1, ZHANG Liu-Suo1, KONG Xiao-Yu1, SONG Lin-Sheng2
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1.Key Laboratory of Mariculture, Ministry of Education , Ocean University of Qingdao;2.Institute of Oceanology , The Chinese Academy of Sciences
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| Abstract: |
| DNA sequencing is very useful for the genetic diversity analysis at molecular level. Mitochondrial 16S rRNA gene fragment of Scallop Chlamys farreri was amplified via polymerase chain reaction (PCR) , the PCR products were ligated into T-vector, cloned and sequenced for 31 individuals, 3 populations of China ( 8 for Dalian and Qingdao each, and 7 for Yantai) and one population of Republic of Korea. 634 base-pair nucleotide sequences were examined and analyzed for genetic polymorphism. Resultant sequences data analysis showed that all 31 sequences were grouped into 18 haplotypes and there were 26 variable nucleotide positions in this gene fragment, which included transitions and transvertions, and no insertion/ deletion were observed. The genetic diversity indexes including S ( polymorphic sites) , P ( nucleotide diversity) and K ( average number of nucleotide difference) were calculated and the results indicated that Korea population showed more variation than others and more differences to others, followed by Yantai and Qigndao population, and Dalian population had the least sequence variation. Parameters of genetic variation among the four populations suggested that no significant genetic difference was observed. Our data provided baseline information at DNA level on the genetic variation in wild Scallop populations and may be useful for future management and utilization of the resource. |
| Key words: Chlamys farreri, Mitochondrial DNA (mtDNA) , 16S rRNA gene, DNA sequencing |
