| 摘要: |
| 目的：制备卵巢过度刺激综合征（OHSS）倾向多囊卵巢综合征（PCOS）大鼠模型,并对模型动物血管通透性进行评价。方法：将23日龄SD雌性大鼠50只随机分为空白对照组、OHSS对照组、高剂量人绒毛膜促性腺激素（hCG）模型组（简称高hCG组）,中剂量hCG模型组（简称中hCG组）、低剂量hCG模型组（简称低hCG组）,每组各10只。高、中、低hCG组大鼠予以颈背部皮下注射脱氢表雄酮（DHEA）制备OHSS倾向PCOS大鼠模型；同期,空白对照组予大鼠颈背部皮下注射中性茶油；OHSS对照组于73日龄至76日龄连续4 d皮下注射妊娠雌马血清促性腺激素（PMSG）,77日龄（PMSG注射后24 h）予皮下注射hCG 1次,建立OHSS模型。各组大鼠分笼饲养至79日龄,于动情前期处死并采集标本,观察大鼠卵巢外观的改变,比较各组大鼠腹水评分,腹腔伊文思蓝（EB）、卵巢EB含量,血清雌二醇（E2）、孕酮（P）、白细胞介素-6（IL-6）、血管内皮生长因子（VEGF）水平。结果：高、中、低hCG组各评价指标出现不同程度升高,提示模型大鼠血管通透性增加。其中,与空白对照组相比,高hCG组各项指标差异均有统计学意义（P＜0.05）；中hCG组除腹水评分外各项指标差异均有统计学意义（P＜0.05）；低hCG组除腹水评分、血VEGF外,其余各指标差异均有统计学意义（P＜0.05）。与OHSS对照组相比,高hCG组腹腔EB、血清E2和P差异均有统计学意义（P＜0.05）；中、低hCG组各项指标差异均有统计学意义（P＜0.05）。结论：DHEA联合中剂量hCG可以诱导OHSS倾向PCOS大鼠模型,模型大鼠的腹腔血管通透性显著增加,但未出现明显腹水,接近人类OHSS倾向PCOS患者的改变。 |
| 关键词: 卵巢过度刺激综合征 多囊卵巢综合征 血管通透性 人绒毛膜促性腺激素 模型制备 |
| DOI： |
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| Influence of human chorionic gonadotrophin dose on vascular permeability in a rat model of polycystic ovary syndrome with a tendency of ovarian hyperstimulation syndrome |
| LIU Xia,ZENG Yiwen,YANG Meichun |
| (Zhaoqing Medical College,Zhaoqing 526020,Guangdong,China;Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China) |
| Abstract: |
| Objective：To establish a rat model of polycystic ovary syndrome (PCOS) with a tendency of ovarian hyperstimulation syndrome (OHSS),and to investigate vascular permeability in model rats.Methods：A total of 50 female Sprague-Dawley rats,aged 23 days,were randomly divided into blank control group,OHSS control group,high-dose human chorionic gonadotrophin (hCG) model group (high-hCG group),middle-dose hCG model group (middle-hCG group),and low-dose hCG model group (low-hCG group),with 10 rats in each group.The rats in the high-,middle-,and low-hCG groups were given subcutaneous injection of dehydroepiandrosterone (DHEA) at the neck and back to establish a model of PCOS with a tendency of OHSS,and meanwhile,the rats in the blank control group were given subcutaneous injection of neutral tea oil at the neck and back；the rats in the OHSS control group were given subcutaneous injection of pregnant mare serum gonadotropin (PMSG) for 4 consecutive days from the age of 73 days to 76 days,followed by a subcutaneous injection of hCG at the age of 77 days (24 hours after PMSG injection),to establish a model of OHSS.The rats were fed to the age of 79 days in separate cages and were sacrificed in proestrus to collect specimens.The change in ovarian appearance was observed,and the above groups were compared in terms of ascites score,Evans blue (EB) content in abdominal cavity and ovary,and serum levels of estradiol (E2),progesterone (P),interleukin-6 (IL-6),and vascular endothelial growth factor (VEGF).Results：The high-,middle-,and low-hCG groups showed varying degrees of increases in the above indices,suggesting an increase in vascular permeability.There were significant differences in these indices between the high-hCG group and the blank control group (P<0.05),and there were no significant differences between the middle-hCG group and the blank control group in all indices except ascites score (P<0.05)；there were significant differences between the low-hCG group and the blank control group in all indices except ascites score and VEGF (P<0.05).There were significant differences in EB content in abdominal cavity and the serum levels of E2 and P between the high-hCG group and the OHSS control group (P<0.05),and there were significant differences in the above indies between the OHSS control group and the middle/low-hCG group (P<0.05).Conclusion：DHEA combined with middle-dose hCG can induce a significant increase in vascular permeability in a rat model of PCOS with a tendency of OHSS,but without ascites,which is similar to the change in PCOS patients with a tendency of OHSS. |
| Key words: ovarian hyperstimulation syndrome polycystic ovary syndrome vascular permeability human chorionic gonadotrophin model establishment |
