| 摘要: |
| 为探究银鲳(Pampus argenteus)应对高温胁迫的生理响应及其相关基因表达研究，本研究设置4个温度组: 对照组26 ℃和实验组28、30、32 ℃。并对银鲳的肝、肾和鳃的组织结构、肝组织中超氧化物歧化酶、过氧化氢酶的相对活性变化和丙二醛含量变化以及hsp 70基因的相对表达量的变化进行了研究。结果显示: (1)随温度的上升，3种组织的组织结构损伤程度逐步加深，且肝、鳃组织表现更为明显且复杂；(2)高温显著影响了银鲳抗氧化酶活性，短期热胁迫时，银鲳对28 ℃能逐步适应，而在30 ℃和32 ℃高温胁迫下，则会对银鲳机体产生不可逆的损伤；(3)随应激时间的延长，不同温度下hsp 70基因的表达量均呈先上升后下降的变化趋势，均在0.5天时显著升高并达到峰值，之后迅速回落。研究表明: 短期热胁迫时，银鲳对28 ℃能逐步适应，而在30 ℃下，胁迫将对其产生严重损伤；32 ℃高温胁迫下，银鲳表现出强烈的应激响应,胁迫对其造成致命损伤。 |
| 关键词: 海洋生物学 银鲳 高温胁迫 组织损伤 酶活性 hsp 70基因表达 |
| DOI：10.3969/J.ISSN.2095-4972.2022.01.001 |
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| 基金项目:国家自然科学基金资助项目(31872586)； 浙江省自然科学基金资助项目(LY18C190008)；浙江省重大科技专项资助项目(2019C02059) |
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| Effects of high temperature stress on physiology and expression of related gene in Pampus argenteus |
| SHI Chenyu,ZHAO Chunpu,HU Yixiao,SHA Taoping,WANG Yajun,WANG Danli,XU Shanliang |
| (School of Marine Sciences, Ningbo University, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China) |
| Abstract: |
| To investigate the physiological responses and related gene expression ofPampus argenteusunder high temperature stress, four temperature treatments, control group (26 ℃) and experimental groups (28, 30, 32 ℃), were set up for the experiment. The changes of tissue structure in liver, kidney and gill, the enzyme activities of superoxide dismutase (SOD) and catalase (CAT), the content of malondialdehyde (MDA) and the relative expression level of hsp 70 gene were studied. The results showed that (1) with increasing temperature, the tissue structure damaged gradually and worsened, particularly for the tissues of liver and gill. (2) The high temperature significantly affected the antioxidant activity of the fish showing irreversible damage at 30 ℃ and 32 ℃ except for the treatment of 28℃ where the fish was getting adapted. (3) When the stress extended, the expression levels of hsp 70 gene in all treatments increased significantly at first and reached a peak in 0.5 d and then decreased quickly. It shows that under short-term heat stress, P. argenteus gradually adapted to 28 ℃ while under high temperature stress of 30 ℃ and 32 ℃ irreversible and deadly damage occurred in the fish body. |
| Key words: marine biology Pampus argenteus high temperature stress tissue damage enzyme activity hsp 70 gene expression level |
