| 摘要: |
| 目的：探讨efa②对刀豆蛋白（con-A）诱导的自身免疫性肝炎小鼠模型的保护作用及其可能的作用机制。方法：将50只昆明种小鼠随机分为正常对照组、con-A自身免疫性肝炎模型组及样品efa②高（0.04mL/20g）、中（0.02mL/20g）、低（0.01mL/20g）剂量组，分别给予0.9%氯化钠注射液或相应浓度的efa②样品灌胃，每天1次，连续7d。本次灌胃结束禁食1晚后，尾静脉注射Con-A，断头取血测生化指标，并取肝右叶组织作病理切片，对肝脏组织中的CYP5A1、CYP2E1和CYP3A进行蛋白分析。结果：efa②能使自身免疫性肝炎模型小鼠血清中IL-10升高以及IFN-γ、TNF-α、PGE1活性降低。结论：efa②具有调节肝炎模型小鼠血清各细胞因子及ALT的水平，并显著改善肝脏组织病理变化的作用，其保护机制可能与CYP2E1相关。 |
| 关键词: 自身免疫性肝炎 小鼠 efa② 刀豆蛋白 实验研究 |
| DOI： |
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| Protective effect of essential fatty acids against liver injury induced by concanavalin-A in mice |
| PENG Qiu-xian，,Lai Lily，,Yung Ken Kinlam |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;Natur-Tech Pharmacal Co.Ltd.，Hong Kong 999077，China;HKBU Science Consultancy Company Limited，Hong Kong 999077，China) |
| Abstract: |
| Objective：To investigate the protective effect of essential fatty acids（EFA）against liver injury induced by concanavalin-A（con-A）in mice and the possible mechanism of action.Methods：A total of 50 Kunming mice were randomly divided into normal control group，con-A-induced autoimmune hepatitis model group，and high-，medium-，and low-dose EFA groups（0.04，0.02，and 0.01mL/20g）and were treated with 0.9% sodium chloride injection or EFA at the corresponding dose by gavage once a day for 7 consecutive days.Fasting was performed for one night after the last gavage，and con-A was injected via the caudal vein.The rats were decapitated and blood samples were collected to measure related biochemical parameters.The tissue of the right lobe of the liver was collected to prepare pathological sections，and CYP5A1，CYP2E1，and CYP3A in liver tissue were analyzed.Results：In mice with autoimmune hepatitis，EFA increased the serum level of interleukin-10 and reduced the activities of interferon gamma，tumor necrosis factor-α，and prostaglandin E1.Conclusion：EFA has a regulatory effect on mice with the serum cytokine of hepatitis and can significantly improve liver pathological changes.Its protective effect may be associated with CYP2E1. |
| Key words: autoimmune hepatitis mouse essential fatty acids concanavalin-A experimental study |
