| 摘要: |
| 目的：研究月华丸（胶囊）对小鼠单核巨噬细胞（RAW264.7）活性的影响，为后续研究月华丸（胶囊）对耐多药结核分支杆菌感染细胞自噬的影响提供前期实验基础。方法：取对数生长期的RAW264.7细胞，用10%胎牛血清培养制成的细胞悬液加入96孔培养板中培养。分别采用终浓度为1.25g/mL、0.63g/mL、0.32g/mL、0.16g/mL、0.125g/mL、0.063g/mL、0.032g/mL、0.016g/mL不含阿胶的月华丸（胶囊）以及相同浓度梯度含阿胶的月华丸（胶囊）处理RAW264.7细胞，并设不加药的对照组。以4-甲偶氮唑蓝（MTT）法检测细胞存活情况。结果：（1）不含阿胶的月华丸（胶囊）在1.25g/mL、0.63g/mL浓度时对RAW264.7细胞增殖抑制率明显升高，与对照组比较差异有统计学意义（P<0.05），表明不含阿胶的月华丸（胶囊）高浓度对巨噬细胞的增殖有一定的抑制作用。（2）含阿胶的月华丸（胶囊）对RAW264.7细胞增殖率明显升高，各浓度组与对照组比较差异均有统计学意义（P<0.05），提示含阿胶的月华丸（胶囊）对巨噬细胞的增殖有明显促进作用。（3）浓度为0.32g/mL的含阿胶月华丸（胶囊）对RAW264.7细胞增殖率最高，为最佳促细胞增长药液浓度。结论：月华丸（胶囊）能提高巨噬细胞的活力，增强巨噬细胞的增殖。 |
| 关键词: 月华丸（胶囊） 单核巨噬细胞 细胞增殖 MTT 实验研究 |
| DOI： |
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| Effect of Yuehua Pill(capsules) and its decomposed formulas on the proliferation of macrophages |
| NING Shang-sheng，,WU Can-rong，,OUYANG Jian-jun， |
| (Hunan University of Traditional Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Yuehua pills/capsules on the viability of mouse mononuclear macrophages（RAW264.7），and to provide a preliminary experimental basis for the research on the effect of Yuehua pills/capsules on the autophagy of cells with multidrug-resistant Mycobacterium tuberculosis infection.Methods：RAW264.7 cells in the logarithmic growth phase were selected，and cell suspension was prepared with 10% fetal bovine serum and then cultured in a 96-well plate.RAW264.7 cells were treated with Yuehua pills/capsules with or without donkey-hide gelatin at a final concentration of 1.25，0.63，0.32，0.16，0.125，0.063，0.032，and 0.016g/mL，respectively，and a control group with untreated cells was established.MTT assay was used to measure cell viability.Results：Yuehua pills/capsules without donkey-hide gelatin had a significant increase in the inhibition rate of RAW264.7 cell proliferation at a concentration of 1.25 and 0.63 g/ml，and there was a significant difference between these two concentration groups and the control group（P<0.05），suggesting that Yuehua pills/capsules without donkey-hide gelatin at a high concentration had a certain inhibitory effect on the proliferation of macrophages.Yuehua pills/capsules with donkey-hide gelatin had a significant increase in the inhibition rate of RAW264.7 cell proliferation and there was a significant difference between each concentration group and the control group（P<0.05），suggesting that Yuehua pills/capsules with donkey-hide gelatin significantly promoted the proliferation of macrophages.RAW264.7 cells treated with Yuehua pills/capsules with donkey-hide gelatin at a concentration of 0.32g/mL had the highest proliferation rate，suggesting that this was the optimal concentration for cell growth promotion.Conclusion：Yuehua pills/capsules can increase the viability of macrophages and promote their proliferation. |
| Key words: Yuehua pill/capsule mononuclear macrophage cell proliferation MTT assay experimental study |
