利用AFLP技术研究条斑紫菜的遗传变异

杨锐; 刘必谦; 骆其君; 王亚军; 包佳美

利用AFLP技术研究条斑紫菜的遗传变异

1.
中国科学院, 海洋研究所, 实验海洋生物学重点实验室, 山东, 青岛, 266071;宁波大学, 海洋生物工程重点实验室, 浙江, 宁波, 315211
2.
宁波大学, 海洋生物工程重点实验室, 浙江, 宁波, 315211

基金项目: 浙江省重点项目(2004C22045);浙江省自然科学基金(M403015);宁波市重点博士基金资助项目(02J2011015)

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出版历程
- 收稿日期: 2003-07-08
- 修回日期: 2003-12-30

Genetic variation of Porphyra yezoensis by using AFLP

1.
Key Laboratory of Experimental Marine Biology, Institite of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China
2.
Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China

摘要

摘要: 利用AFLP技术对11个条斑紫菜品系进行了研究.由16对引物共扩增出778条谱带,其中仅15条为共有谱带,多态位点的比例高达98.07%.日本鹿儿岛,我国的青岛、江苏、浙江等不同地点的野生或栽培条斑紫菜品系之间最近的遗传距离为0.180(两个日本样本之间),最远为0.397(日本样本与江苏样本之间),属于物种内种群间的遗传变异.聚类结果显示日本的样本与青岛的野生样本间的遗传相似系数较高而聚合在一起;浙江野生条斑紫菜与江苏的样本遗传距离相近,聚合在一起.结果表明,我国条斑紫菜的遗传多样性程度较高而且各品系间的遗传距离与其地理间距呈正相关关系.
- 条斑紫菜 /
- AFLP /
- 遗传变异
Abstract: Genetic variation of 11 lines of Porphyrayezoensis from the coast seas of Kagoshima (Japan),Qingdao,Nantong,Putuo and Naiji islands were studied by using amplified fragment length polymorphism (AFLP).778 bands are obtained with AFLP analysis of 16 primer combinations,among which 15 are unique,about 98.07% are polymorphic.The AFLP data show that the closest genetic distance are 0.180 between two Kagoshima samples,and the furthest one 0.397 is between Kagoshima No.1 line and Nant ng No.9 line.The genetic distance shows that the variation is within the inner species scope.Neighborjoining cluster and UPGMA cluster indicate that samples from Kagoshima and Qingdao were with high similarity and either with the samples of Nantong,Putuo and Nanjiislands.Porphyrayezoensis in China shares high genetic diversity,and the genetic distance shows positive correlation with the geographic distance.
- Porphyrayezoensis /
- AFLP /
- genetic variation

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参考文献(11)

YOSHIDA T,NOTOYA M,KIKUCHI N,et al.Catalogue of species of Porphyra in the world,with special reference to the type locality and bibliography[J].Nat HisRes(special issue),1997,3:5-18.

VOS P,HOGERS R,BLEEKER M,et al.AFLP:a new technique for DNA fingerprinting[J].Nuc Acids Res,1995,23(21):4 407-4 414.

MUELLER U,WOLFENBARGER L l.AFLP genotyping and fingerprinting[J].Tree,1999,14(10):389-394.

GAIOTTO F A,BRAMUCCI M,GRATTAPAGLIA D.Estimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers[J].Thcor Appl Genet,1997,95(5～6):842-849.

PAGLIA G,MORGANTE M.PCR-based multiples DNA fingerprinting techniques for the analysis of conifer genomes[J].Mol Breed,1998,4(2):173-177.

DONALDSO S,CHOPIN T,SAUNDERS G W.Amplified fragment length polymorphism(AFLP) as a source of genetic markers for red algae[J].J Appl Phycol,1998,10(3):365-370.

BASSAM B J,CAETANO-ANOLLES G,GRESSHOFFP M.Fast and sensitive silver staining of DNA in polyacrylamide gels[J].Areal Biochem,1991,196(1):80-83.

MIZUKAMI Y,OKAUCHI M,KITO H,et al,Discrimination of laver cultivars with RAPD markers[J].Fish Sci,1996,62(4):547-551.

王勇,刘必谦,骆其君,等.坛紫菜品系间遗传差异的RAPD分析[J].青岛海洋大学学报,2000,30(2):225-229.

贾建航,王萍,金德敏,等.RAPD标记在紫菜遗传多样性检测和种质鉴定中的应用[J].植物学报,2000,42(4):403-407.

杨锐,刘必谦,骆其君,等.利用扩增片段长度多态性(AFLP)研究坛紫菜的遗传变异[J].高技术通讯,2002,12(1):83-86.

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