活性氧对3种海洋微藻生长的影响
Effect of reactive oxygen species on cell growth of marine microalgae
-
摘要: 以Fe2+/Vc为活性氧诱生系统,对活性氧作用下3种海洋微藻的生长情况进行了研究.结果表明,低浓度的活性氧可以促进3种海洋微藻的生长,使藻细胞的脂质过氧化程度升高,同时刺激细胞的SOD活性使其略有上升,加入抗氧化剂可以抑制其促进作用;高浓度的活性氧能抑制3种海洋微藻细胞的生长,使细胞的脂质过氧化程度过量增高,抑制细胞的SOD活性,抗氧化剂可以缓解其对藻细胞的毒性作用.不同种类的微藻对活性氧的敏感性存在差异.Abstract: Fe2+/Vc system is used to generate hydroxyl radical.The proliferation of marine microalgae under hydroxyl radical stress is studied.The results indicate that the cell growth of microalgae is stimulated,the lipid peroxidation increases in the certain ranges and the activities of SOD of algal cells are enhanced under low concentration of hydroxyl radical stress.The growth of algal cell stimulated by ROS can be inhibited by antioxidant.Under high concentration of hydroxyl radical stress,the proliferation of algal cells is inhibited,the lipid peroxidation increases rapidly and the activities of SOD of cells declines.The antioxidant can alleviate the damage of algal cells under high concentration of hydroxyl radical stress.The sensitivities are different for different species of marine microalgae under ROS.stress.
-
MCCORD J M,FRIDOVICH I.Superoxide dismutase:an enzyme function for erythrocuprein(hemocuprein) [J].J Bio Chem,1969,193:353~358. 方允中,李文杰.自由基与酶[M].北京:科学出版社,1989. 陈恩学,焦新之.植物质膜氧化还原系统的生理作用[J].生命科学,1995,7(4):24~31. 唐学玺,李永祺,李春雁,等.有机磷农药对海洋微藻致毒性的生物学研究I[J].海洋环境科学,1995,14(2):1~5. 唐学玺,李永祺,李春雁,等.有机磷农药对海洋微藻致毒性的生物学研究Ⅱ[J].海洋学报,1997,19(1):140~143. WINSTON G W,DIGIULIO R T.Prooxidant and antioxidant mechanisms in aquatic organisms[J].Aquatic Toxicology,1991,19:137~161. MEHDY M C.Active oxygen species in plant defense against pathogens[J].Plant Physiol,1994,105:467~472. DYPBUKT J M,ANKARCRONA M,BURKITT M,et al.Different prooxidant levels stimulate growth,trigger apoptosis produce necrosis of insulin-secreting RINm5F cells[J].Journal of Biological Chemistry,1994,269(48):30 553~30 560. MURRELL G A C,FRANCIS M J O,BROMLEY L.Modulation of fibroblast proliferation by oxygen free radicals [J].Biochem J,1990,265:659~665. LO Y Y C,CRUZ T F.Involvement of reactive oxygen species in cytokine and growth factor induction of C-fos expression in chondrocytes[J].Journal of Biological Chemistry,1995,270:11 727~11 730. 任建国,李建峰,郑荣梁.氧胁迫对人肝癌细胞生长分化和凋亡的影响[J].实验生物学报,1998,31(3):273~278. 郑铁松,郑荣梁.脂质过氧化对细胞衰老的延缓作用(Ⅱ)[J].实验生物学报,1994,27(2):205~209. HALLIWELL B,GUTTERIDGE J M C.Role of free radicals and catalytic metal ions in human disease:an overview[A].Packer L,Glazer A N.Methods in Enzymology[M].San Diego:Academic Press,1986.1~85. BEACHAMP C,FRIDOVICH I.Superoxide dismutase:improved assays and an assay applicable to acrylamide gel[J].Anal Biochem,1971,44:246~278. BEWLEY T D.Physiological aspects of desication tolerance[J].Ann Rev Plant Physiol,1979,30:195~238. HEATH R L,PACKER L.Photoparoxidation in isolated chloroplasts.Kinetics and stoichiometry of fatty acid peroxidation[J].Arch Biochem Biophys,1981,125:189~198. 林植芳,李双顺,林桂珠,等.水稻叶片的衰老与超氧化物歧化酶活性及脂质过氧化作用的关系[J].植物学报,1984,26(6):605~615. 陈瑗,周玫.氧化应激与cfos和cjun转录和AP-1激活[J].生物化学与生物物理进展,1995,22(5):386~390. BUONAURIO R,TORRE G D,MONTALBINI P.Soluble superoxide dismutase(SOD) in susceptible and resistant hostparasite complex of Paseolus vulgaris and Uromyces phaseoli [J].Physiol Mol Plant Pathol,1987,31:173. 李培峰,方允中.抗坏血酸-Fe3+对牛红细胞铜锌超氧化物歧化酶氧化修饰作用[J].生物化学与生物物理学报,1994,26(3):263~270.
点击查看大图
计量
- 文章访问数: 768
- HTML全文浏览量: 31
- PDF下载量: 970
- 被引次数: 0