As one of the essential trace elements in human bodies, zinc has close relationship with the occurrence and development of diseases. Up till now, the distribution of zinc in pathological mineralization is still obscure. This paper focuses on the mineralization of breast cancer, fibroadenoma of breast, breast hyperplasia, breast inflammation, ovarian serous cancer, aortic calcification and coronary artery calcification, based on 28 cases. After the mineral separation process, the measurement of element content was carried out by using micro-area synchrotron radiation X-ray fluorescence (μ-SRXRF). μ-SRXRF mapping was also conducted to determine the distribution of zinc and calcium. The distribution of zinc in all the types of diseases studied is generally consistent with that of calcium, but shows opposite trend in some parts, similar to the Sr-Ca relationship. Therefore, Zn might occupy the place of Ca in the crystal lattices of (carbonate) hydroxyapatite. Besides, Ca/Zn (mM concentration) values of the mineralization in breast cancer is significantly different from those in breast benign lesions (P<0.01), because the concentration of Zn in breast cancer is higher than that of breast benign lesions.