Micro_CT technology and LA_ICP_MS were applied to studying the response relationship between carp otolith and heavy metals in waters. The LA_ICP_MS analysis has revealed that the contents of Cu and Pb of otoliths in Baiyangdian are higher than the contents of corresponding elements of otoliths in the Miyun reservoir, and the Micro_CT data show that the Micro_CT value of otolith from the Baiyangdian is higher than that from the Miyun Reservoir. The authors consider that the contents of Cu and Pb seem to be the main factors responsible for the difference of Micro_CT values of otolith from different waters. Elements in otolith show similar trend and enrichment to the corresponding elements in the water. It is thus inferred that there exists a coupling relationship between heavy metals in water and Micro_CT data, which can be used as a typomorphic characteristic to indicate the information of heavy metals in water.