The sedimentary sequence and geochemistry of manganesebearing strata in Huangtukan (Zunyi) and Yingpan (Nayong) were studied. The results show that the manganese deposit in Yingpan and Huangtukan occurs as stratified, stratoid beds, lenticular, and vein, and is of brecciated structure and graded bedding structure, etc, and intercalated with tuff and siliceous rocks. Therefore, the Permian manganese deposit in Guizhou Province is hydrothermal in origin. Mnbearing layer is sandwiched between overlying basalt and underlying Maokou Formation limestone, demonstrating that the manganese deposit formed prior to eruption of basalt magma. Chalcopyrite, pyrite, barite, celestite, rhodochrosite, calcium rhodochrosite, calcimangite, azurite, limonite, chlorite, quartz and carbonate minerals are disseminated in the Permian manganesebearing strata. The mineral association is similar to hydrothermal mineral association. The analyses of major elements, trace elements, rare earth elements, along with C isotopic of manganesebearing strata, indicate that Mnbearing deposit is enriched in As, Co, Cu, Cr, Mo, Ni, Pb, U, V, etc. Ratios of Fe/Ti, (Fe+Mn)/T and Al/(Al+Fe+Mn), and FeMn(Cu+Co+Ni)×10 and ZrCr diagrams show that the manganese deposit is hydrothermal deposition genesis. C and O isotopic compositions of the manganese deposit are 417‰~-1853‰PDB, and -698‰~-1005‰PDB, respectively, implying that the manganese deposit is hydrothermal deposition in origin also. REE pattern curves of manganese deposits in Huangtukan and Yingpan are all overlapped with that of E’meishan Basalt, suggesting that the oreforming material of the manganese deposit is closely related to the E’meishan mantle plume.