The Middle-Lower Yangtze River Valley Mineralization Belt (MLYB) is one of the most significant polymetallic belts in China. Based on available satellite-derived free-air gravity anomalies and elevation database sets, this study obtained the 3D Moho pattern of the MLYB and Its adjacent areas using Parker-Oldenburg wave number domain iteration method. Combined with existing research results, the deep tectonic framework in the study area was also addressed. The results show that the Moho depth generally presents a decreasing trend from southwest to northeast in the study area, with the shallowest location (28~29km) between the Yangtze Estuary and the Hangzhou Bay sea, and the deepest (38~39km) within the Dabie Mountain. The deepest location of the Moho uplifting is near the turning point of V shape, with two sides of the Moho lifted. The uplift amplitude and scale of the NEtrending eastern branch of the V shape is apparently greater than that of the NWtrending west branch. Satellite magnetic anomalies indicate that high positive magnetic anomaly values in the MLYB concentrate on volcanic rocks basins (such as Ningwu, Luzong, Fanchang and Huaining) in the eastern branch of V shape. The Moho in the eastern section of QinHang metallogenic belt also uplifted notably, with the center of “mantle uplift” distributed along the southeast of the fault in the south boundary of the metallogenic belt. The striped uplift zone of the Moho within the MLYB and also the eastern section of the QinHang metallogenic belt possibly indicates mantle uplifting and magma upwelling, as well as the deep boundary of underplating between crustmantle and lower crust. Meanwhile, orebearing magma from the lower crust or mantle migrated upwards along deep faults of the brittle crust, and precipitated in favorable positions to form metallic ore cluster areas.