| 白斑综合症病毒膜蛋白VP124参与病毒的感染 |
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| 引用本文： | 朱艳冰,吴成林,杨丰.白斑综合症病毒膜蛋白VP124参与病毒的感染[J].海洋学报,2008,30(1):135-139. |
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| 作者姓名： | 朱艳冰 吴成林 杨丰 |
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| 作者单位： | 1.集美大学, 生物工程学院, 福建, 厦门, 361021 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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福建科学基金 |
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| 摘 要： | 白斑综合症病毒(white spot syndrome virus,WSSV)是对虾的最主要病毒病原之一,给对虾养殖造成了巨大的经济损失。WSSV分别与三种该病毒膜蛋白VP39,VP124和VP187的特异性抗血清在体外作用后,再注射到螯虾体内,进行中和试验。结果显示,注射同或未同VP124抗血清作用的WSSV,8 d后螯虾的死亡率分别是100%和60%,WSSV的感染能够被抗VP124抗体中和。进一步利用定量PCR分析上述三种特异性抗血清对病毒感染的影响,结果显示,VP124抗血清可抑制WSSV在螯虾体内的增殖。所有结果表明,VP124在病毒的感染中起作用。
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| 关 键 词： | 白斑综合症病毒 膜蛋白VP124 抗血清 感染 中和试验 |
| 文章编号： | 0253-4193(2008)01-0135-05 |
| 收稿时间： | 2006/6/28 0:00:00 |
| 修稿时间： | 2006年6月28日 |
White spot syndrome virus envelope protein VP124 is involved in the virus infection |
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| ZHU Yan-bing,WU Cheng-lin and YANG Feng.White spot syndrome virus envelope protein VP124 is involved in the virus infection[J].Acta Oceanologica Sinica (in Chinese),2008,30(1):135-139. |
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| Authors: | ZHU Yan-bing WU Cheng-lin and YANG Feng |
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| Affiliation: | 1.School of Biotechnology, Jimei University, Xiamen 361021, China2.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: | White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses for shrimp farming.In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually.And then they were injected intramuscularly into crayfish to conduct in vivo neutralizationassays.The results showed that for groups injected with virions only and groups injected with virions and the VP124 antiserum mixture, the crayfish mortalities were 100% and 60% at 8 day spost-infection, individually.The virus infection could be neutralized by antibody against the envelope protein VP124.Quantitative PCR were used to further investigate the influence of three antisera above described on the virus infection.The results showed that the VP124 antiserum couldrestrainthe propagation of WSSV in cray fish.All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection. |
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| Keywords: | white spot syndrome virus envelope protein VP124 antibody infection neutralization assay |
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