| 氧含量及附着面积对海洋底栖硅藻生长和胞外多聚物的影响 |
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| 引用本文： | 陈琪,武福源,郑纪勇,杨靖亚,蔺存国.氧含量及附着面积对海洋底栖硅藻生长和胞外多聚物的影响[J].海洋学研究,2017,35(1):66-72. |
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| 作者姓名： | 陈琪 武福源 郑纪勇 杨靖亚 蔺存国 |
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| 作者单位： | 1.海洋腐蚀与防护重点实验室,山东 青岛 266101; 2.中国船舶重工集团公司 第七二五研究所,河南 洛阳 371000; 3.上海海洋大学 食品学院,上海 201306 |
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| 基金项目： | 国家重点基础研究发展计划项目资助(2014CB643305);国家自然科学基金项目资助(21301161) |
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| 摘 要： | 胞外多聚物(extracellular polymeric substance, EPS)是海洋底栖硅藻在水下表面附着时分泌的物质,环境因素对胞外多聚物的组成和分泌行为有直接影响,本文重点探讨了氧含量和附着面积对胞外多聚物的影响。采用热溶剂浸提法提取硅藻胞外多聚物,利用苯酚-硫酸法定量其中的多糖成分,利用考马斯亮蓝G-250法定量蛋白质成分,通过血球计数板法和紫外分光光度法计算细胞数量。结果表明,在氧含量高、可附着面积大的条件下,硅藻的生长繁殖速率快,且单个细胞的胞外多聚物分泌量大。氧含量对胞外多聚物主要成分中的水溶性多糖含量影响大,而对其余成分含量影响小。可附着面积对硅藻附着和生长的影响比氧含量影响小。
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| 关 键 词： | 海洋底栖硅藻 氧含量 附着面积 胞外多聚物 |
| 收稿时间： | 2016-02-24 |
Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom |
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| CHEN Qi,WU Fu-yuan,ZHENG Ji-yong,YANG Jing-ya,LIN Cun-guo.Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom[J].Journal of Marine Sciences,2017,35(1):66-72. |
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| Authors: | CHEN Qi WU Fu-yuan ZHENG Ji-yong YANG Jing-ya LIN Cun-guo |
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| Affiliation: | 1. State Key Laboratory for Marine Corrosion and Protection, Qingdao 266101, China; 2. Luoyang Ship Material Research Institute, Luoyang, 371000, China; 3. Food Science and Technology College, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | The extracellular polymeric substance (EPS) was secreted when marine benthic diatom attached on submerged surfaces, the environment factors had a directly impact on the composition and secretion action of EPS. The effects of the oxygen content and the attachment area on EPS were studied. The EPS from marine benthic diatoms were extracted by hot solvent method. The phenol-sulfuric acid method was employed to measure the content of polysaccharide and the Coomassie Brilliant Blue G-250 method was employed to measure the content of protein in the extractions. The cell number of diatoms was measured through the method of blood cell counting plate and UV spectrophotometry. The results show that the growth rate of diatoms is fast and the secretion amounts of EPS in a single cell are great under the higher oxygen content and larger attachment area conditions. The oxygen content has a great impact on the content of water-soluble polysaccharide as the main composition in EPS, while it has little impact on the else components. Compared to the oxygen content, the attachment area has little impact on the attachment and growth of diatoms. |
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| Keywords: | marine benthic diatom oxygen content attachment area extracellular polymeric substance |
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