| RAPD技术在中国对虾群体鉴别上的应用 |
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| 引用本文： | 刘振辉,孔杰,刘萍,邓景耀.RAPD技术在中国对虾群体鉴别上的应用[J].海洋学报,2003,25(4):67-71. |
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| 作者姓名： | 刘振辉 孔杰 刘萍 邓景耀 |
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| 作者单位： | 1.中国海洋大学, 海洋生命学院, 山东, 青岛, 266003 |
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| 基金项目： | 国家“973”计划资助项目(G1999012007) |
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| 摘 要： | 采用RAPD技术对中国对虾(Penaeus chinensis)、中国黄海、渤海沿岸种群(H)朝鲜半岛西海岸种群(K)和即墨健康养殖群体(S)的DNA进行检测,41个随机引、物在3个群体间共筛选到了3个稳定的RAPD标记F-02540,OPN-13800和OPN-202200,可用于群体间的鉴别,研究将为中国对虾分子标记遗传育种提供依据。
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| 关 键 词： | 中国对虾 RAPD技术 遗传标记 |
| 文章编号： | 0253-4193(2003)04-0067-05 |
| 收稿时间： | 2000/11/7 0:00:00 |
| 修稿时间： | 2000年11月7日 |
Application of RAPD technology for identification in three different stocks of Penaeus chinensis |
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| LIU Zhen-hui,KONG Jie,LIU Ping and DENG Jing-yao.Application of RAPD technology for identification in three different stocks of Penaeus chinensis[J].Acta Oceanologica Sinica (in Chinese),2003,25(4):67-71. |
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| Authors: | LIU Zhen-hui KONG Jie LIU Ping and DENG Jing-yao |
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| Affiliation: | 1.College of Marine Biology Science, Ocean University of China, Qingdao 266003, China2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| Abstract: | Three stocks (K, H and S) of Penaeus chinensis were analysed using 41 random oligonucleotide primers with 10 bases by RAPD method. K was from the spawning stock in the western coastal waters of Korea Peninsula; H was from the Huanghai (Yellow) Sea-Bohai Sea stock; S was from the health cultured stock in Jimo, Shandong Province. Three genetic markers which can identify the three stocks were got in this study. The study may provide the basic materials for marker assisted selection (MAS) of Penaeus chinensis. |
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| Keywords: | Penaeus chinensis RAPD technology genetic marker |
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