| 海水酸化对厚壳贻贝(Mytilus coruscus)外套膜代谢组的急性影响 |
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| 引用本文： | 范孝俊,王莹,蔡吕萍,黄键锋,刘菲,李迎澳,张晓林,严小军,廖智.海水酸化对厚壳贻贝(Mytilus coruscus)外套膜代谢组的急性影响[J].海洋与湖沼,2023,3(3):921-933. |
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| 作者姓名： | 范孝俊 王莹 蔡吕萍 黄键锋 刘菲 李迎澳 张晓林 严小军 廖智 |
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| 作者单位： | 浙江海洋大学海洋科学与技术学院 海洋生物资源与分子工程实验室 浙江 舟山 316022 |
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| 基金项目： | 国家自然科学基金重点国际合作与交流项目,42020104009号；国家自然科学基金面上项目,32271580号；舟山市科技局计划专项项目,2019F12004号 |
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| 摘 要： | 海洋酸化是当前全球面临的最为紧迫的环境问题之一，已显现出对海洋生物的严重影响。贻贝是我国重要的经济贝类之一，但目前尚缺乏海洋酸化背景下厚壳贻贝的分子响应相关研究。为此，对厚壳贻贝外套膜组织开展了急性酸化条件下的代谢组学分析，以期了解厚壳贻贝在酸化条件下，其外套膜组织的代谢物组成和含量变化。采用超高压液相色谱-串联质谱技术，结合代谢物数据库搜索和鉴定，从厚壳贻贝外套膜中鉴定代谢物总计7882种。与对照组相比，酸化处理后的厚壳贻贝外套膜组织中共有497种代谢物含量发生显著变化(P<0.05)，其中，320种差异代谢物显著上调，177种显著下调。差异代谢物富集的代谢通路主要包括脂代谢、信号转导、维生素代谢、核酸代谢、氨基酸代谢以及ABC转运体。结合外套膜游离氨基酸组成分析以及抗氧化活性分析，推测厚壳贻贝通过激活尿素合成、增强细胞膜流动性以及加强渗透压调节和钙离子转运等方式来应对海洋酸化的威胁。上述研究为深入了解贻贝应对海洋酸化的分子策略，以及在海洋酸化大背景下的贻贝健康养殖奠定了基础。
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| 关 键 词： | 厚壳贻贝(Mytilus coruscus) 外套膜 海洋酸化 代谢组学 |
| 收稿时间： | 2022/10/25 0:00:00 |
| 修稿时间： | 2022/12/5 0:00:00 |
EFFECTS OF ACUTE SEAWATER ACIDIFICATION ON METABOLISM OF MANTLE IN MYTILUS CORUSCUS |
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| FAN Xiao-Jun,WANG Ying,CAI Lyu-Ping,HUANG Jian-Feng,LIU Fei,LI Ying-Ao,ZHANG Xiao-Lin,YAN Xiao-Jun,LIAO Zhi.EFFECTS OF ACUTE SEAWATER ACIDIFICATION ON METABOLISM OF MANTLE IN MYTILUS CORUSCUS[J].Oceanologia Et Limnologia Sinica,2023,3(3):921-933. |
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| Authors: | FAN Xiao-Jun WANG Ying CAI Lyu-Ping HUANG Jian-Feng LIU Fei LI Ying-Ao ZHANG Xiao-Lin YAN Xiao-Jun LIAO Zhi |
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| Affiliation: | Laboratory of Marine Biology Resource and Molecular Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan 316022, China |
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| Abstract: | Ocean acidification is one of the most urgent environmental problems over the world and has shown serious impacts on the species with biomineralization process. Mytilus is one of the important economic shellfish in China. However, there is a lack of studies on molecular response of Mytilus coruscus under ocean acidification. Therefore, metabolomics analysis of the mantle tissues of M. coruscus was performed to understand the changes in metabolite composition and content of the mantle tissues of the mussels under acute acidification conditions. Using LC-MS/MS technology, combined with metabolite database search and identification, a total of 7 882 metabolites from the mantle of M. coruscus were identified. Compared with the control group, a total of 497 metabolites were significantly changed in the acidification treatment group (P<0.05), of which 320 metabolites were significantly up-regulated and 177 metabolites were significantly down-regulated. Functional enrichment analysis of differential metabolites revealed that the metabolites were enriched in different biofunctions, including lipid metabolism, signal transduction, vitamin metabolism, nucleic acid metabolism, amino acid metabolism, and ABC transporters. Combined with the analysis of free amino acid composition and antioxidant activity of the mantle, it was speculated that the mussels responded to the threat of ocean acidification by activating urea synthesis, enhancing cell membrane fluidity, and enhancing osmotic pressure regulation and calcium ion transport. These results lay the foundation for further understanding the molecular strategies of mussels to cope with ocean acidification, and for the healthy cultivation of mussels in the context of ocean acidification. |
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| Keywords: | Mytilus coruscus mantle ocean acidification metabolomics |
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