| 应用流式细胞术测定中国四种经济蟹类的基因组大小 |
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| 引用本文： | 刘磊,崔朝霞,宋呈文,刘媛,惠敏,王春琳.应用流式细胞术测定中国四种经济蟹类的基因组大小[J].海洋学报(英文版),2016,35(6):7-11. |
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| 作者姓名： | 刘磊 崔朝霞 宋呈文 刘媛 惠敏 王春琳 |
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| 作者单位： | 宁波大学海洋学院, 宁波 315211,中国科学院海洋研究所, 青岛 266071,中国科学院海洋研究所, 青岛 266071,中国科学院海洋研究所, 青岛 266071,中国科学院海洋研究所, 青岛 266071,宁波大学海洋学院, 宁波 315211 |
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| 摘 要： | The genome size(C-value) of an organism is referring to the DNA content of its non-replicated haploid chromosome complement,generally deduced from measuring somatic diploid nuclei.We presented genome size(C-value) data obtained by flow cytometry for four commercially important crabs(Portunus trituberculatus,Charybdis japonica,Scylla paramamosain,and Eriocheir sinensis) common in the coast of China.Gallus domesticus(2C=2.5 pg) was used as the internal standard.The results showed that the C-value for P.trituberculatus,C.japonica,S.paramamosain,and E.sinensis were(2.31±0.01) pg,(2.33±0.03) pg,(1.64±0.02) pg,and(2.29±0.03) pg,respectively.The C-value of P.trituberculatus,C.japonica and S.paramamosain were reported for the first time.The data represented by the four species indicated that they had lower DNA contents than average DNA values in crustaceans((4.99±0.48) pg),and three of the four values were very similar if not identical.The results provide useful data for future studies in the fields of biodiversity,species conservation,and phylogeny of these commercial crabs.They will also be helpful in instructing the hybridization breeding program and estimating the cost of the whole genome sequencing project.
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| 关 键 词： | 基因组大小 流式细胞术 蟹 梭子蟹科 方蟹科 |
| 收稿时间： | 2015/4/2 0:00:00 |
| 修稿时间： | 2015/12/16 0:00:00 |
Flow cytometric analysis of DNA content for four commercially important crabs in China |
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| LIU Lei,CUI Zhaoxi,SONG Chengwen,LIU Yuan,HUI Min and WANG Chunlin.Flow cytometric analysis of DNA content for four commercially important crabs in China[J].Acta Oceanologica Sinica,2016,35(6):7-11. |
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| Authors: | LIU Lei CUI Zhaoxi SONG Chengwen LIU Yuan HUI Min and WANG Chunlin |
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| Affiliation: | 1.School of Marine Sciences, Ningbo University, Ningbo 315211, China2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | The genome size (C-value) of an organism is referring to the DNA content of its non-replicated haploid chromosome complement, generally deduced from measuring somatic diploid nuclei. We presented genome size (C-value) data obtained by flow cytometry for four commercially important crabs (Portunus trituberculatus, Charybdis japonica, Scylla paramamosain, and Eriocheir sinensis) common in the coast of China. Gallus domesticus (2C=2.5 pg) was used as the internal standard. The results showed that the C-value for P. trituberculatus, C. japonica, S. paramamosain, and E. sinensis were (2.31±0.01) pg, (2.33±0.03) pg, (1.64±0.02) pg, and (2.29±0.03) pg, respectively. The C-value of P. trituberculatus, C. japonica and S. paramamosain were reported for the first time. The data represented by the four species indicated that they had lower DNA contents than average DNA values in crustaceans ((4.99±0.48) pg), and three of the four values were very similar if not identical. The results provide useful data for future studies in the fields of biodiversity, species conservation, and phylogeny of these commercial crabs. They will also be helpful in instructing the hybridization breeding program and estimating the cost of the whole genome sequencing project. |
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| Keywords: | genome size flow cytometry crabs Portunidae Grapsidae |
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