| 分离自中国胶州湾的褐色马格里夫藻(Margalefidinium fulvescens)的形态学和系统进化特征 |
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| 引用本文： | 胡章喜,邓蕴彦,李宇航,TANG Ying Zhong.分离自中国胶州湾的褐色马格里夫藻(Margalefidinium fulvescens)的形态学和系统进化特征[J].海洋学报(英文版),2018,37(10):11-17. |
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| 作者姓名： | 胡章喜 邓蕴彦 李宇航 TANG Ying Zhong |
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| 作者单位： | 中国科学院海洋生态与环境科学重点实验室, 山东青岛 266071;青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东青岛 266237;中国科学院海洋大科学研究中心, 山东青岛 266071,中国科学院海洋生态与环境科学重点实验室, 山东青岛 266071;青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东青岛 266237;中国科学院海洋大科学研究中心, 山东青岛 266071,中国科学院海洋研究所海洋生物分类与系统演化实验室, 山东青岛 266071,中国科学院海洋生态与环境科学重点实验室, 山东青岛 266071;青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东青岛 266237;中国科学院海洋大科学研究中心, 山东青岛 266071 |
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| 基金项目： | The National Natural Science Foundation of China under contract No. 41506143; the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences under contract No. U1606404; the National Natural Science Foundation of China under contract Nos 41476142, 61533011 and 41776125; the Scientific and Technological Innovation Project supported by the Qingdao National Laboratory for Marine Science under contract No. 2016ASKJ02; the Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology under contract No. LMEES-CTSP-2018-1. |
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| 摘 要： | The dinoflagellate genus Margalefidinium has been split from Cochlodinium as a new genus recently and Margalefidinium fulvescens is one of the five Margalefidinium species. Margalefidinium fulvescens is toxic and has been reported from the coastal waters of USA, Canada, Mexico, China, Japan, Indonesia, Korea, Pakistan and Spain. Here we provide the morphological and phylogenetic characterization for an isolate of it from the Jiaozhou Bay, Qingdao, China. Our results showed that the vegetative cells were subspherical to ellipsoidal, 34–60 μm in length, and 19–41 μm in width. Both single cell forms and colonies in chains of 2, 4, or 8 cells were observed in cultures, but chain forms with 2 or 4 cells were observed more often in the field samples. The cingulum was rather deep, encircling the cell approximately twice, but the sulcus was rather narrow, surrounding the cell about one turn. The nucleus was spherical and located at the central epicone. The chloroplasts were granular, brownish, and scattered peripherally. An orange pigmented body also appeared in the epicone. The apical groove appeared vase-like as previously described. Under epi-fluorescence microscopy, a pumpkin-like structure was clearly observed, in which cells were embedded. Cells were observed to exit from the structure, which led us to a hypothesis that the structure may provide cells a shelter to avoid predation or to respond to other stresses. The phylogenetic analyses based on partial LSU rDNA sequences indicated that M. fulvescens from the Jiaozhou Bay was grouped with M. fulvescens populations from other origins and closely related to the clade of M. polykrikoides.Our morphological observations and phylogenetic analyses together confirmed the presence of M. fulvescens in China and our monitoring has also observed the species dominant in the dinoflagellate community of the Jiaozhou Bay in the early autumn of 2015, which alerted us to continually monitor this bloom-forming species in the region.
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| 关 键 词： | 褐色马格里夫藻 形态特征 系统进化 南瓜型结构 胶州湾 中国 |
| 收稿时间： | 2017/6/27 0:00:00 |
The morphological and phylogenetic characterization for the dinoflagellate Margalefidinium fulvescens (=Cochlodinium fulvescens) isolated from the Jiaozhou Bay, China |
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| HU Zhangxi,DENG Yunyan,LI Yuhang and TANG Ying Zhong.The morphological and phylogenetic characterization for the dinoflagellate Margalefidinium fulvescens (=Cochlodinium fulvescens) isolated from the Jiaozhou Bay, China[J].Acta Oceanologica Sinica,2018,37(10):11-17. |
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| Authors: | HU Zhangxi DENG Yunyan LI Yuhang and TANG Ying Zhong |
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| Affiliation: | 1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology,Chinese Academy of Sciences,Qingdao,China;2.Laboratory for Marine Ecology and Environmental Science,Pilot National Laboratory for Marine Science and Technology (Qingdao),Qingdao,China;3.Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao,China;4.Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology,Chinese Academy of Sciences,Qingdao,China |
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| Abstract: | The dinoflagellate genus Margalefidinium has been split from Cochlodinium as a new genus recently and Margalefidinium fulvescens is one of the five Margalefidinium species. Margalefidinium fulvescens is toxic and has been reported from the coastal waters of USA, Canada, Mexico, China, Japan, Indonesia, Korea, Pakistan and Spain. Here we provide the morphological and phylogenetic characterization for an isolate of it from the Jiaozhou Bay, Qingdao, China. Our results showed that the vegetative cells were subspherical to ellipsoidal, 34–60 μm in length, and 19–41 μm in width. Both single cell forms and colonies in chains of 2, 4, or 8 cells were observed in cultures, but chain forms with 2 or 4 cells were observed more often in the field samples. The cingulum was rather deep, encircling the cell approximately twice, but the sulcus was rather narrow, surrounding the cell about one turn. The nucleus was spherical and located at the central epicone. The chloroplasts were granular, brownish, and scattered peripherally. An orange pigmented body also appeared in the epicone. The apical groove appeared vase-like as previously described. Under epi-fluorescence microscopy, a pumpkin-like structure was clearly observed, in which cells were embedded. Cells were observed to exit from the structure, which led us to a hypothesis that the structure may provide cells a shelter to avoid predation or to respond to other stresses. The phylogenetic analyses based on partial LSU rDNA sequences indicated that M. fulvescens from the Jiaozhou Bay was grouped with M. fulvescens populations from other origins and closely related to the clade of M. polykrikoides. Our morphological observations and phylogenetic analyses together confirmed the presence of M. fulvescens in China and our monitoring has also observed the species dominant in the dinoflagellate community of the Jiaozhou Bay in the early autumn of 2015, which alerted us to continually monitor this bloom-forming species in the region. |
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| Keywords: | Margalefidinium fulvescens morphology phylogeny pumpkin-like structure Jiaozhou Bay China |
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