| Development of a real-time PCR method for Thalassiosira rotula rapid detection |
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| 作者姓名： | HE Shanying YU Zhigang MI Tiezhu |
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| 作者单位： | HE Shanying1，YU Zhigang1，MI Tiezhu21. College of Chemistry and Chemical Engineering，Ocean University of China，Qingdao 266003，China2. College of Environmental Science and Engineering，Ocean University of China，Qingdao 266003，China |
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| 基金项目： | 国家自然科学基金
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国家高技术研究发展计划(863计划) |
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| 摘 要： | Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.
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| 关 键 词： | 圆海链藻 赤潮 快速检测 实时PCR法 18S rDNA |
| 收稿时间： | 2006-03-07 |
| 修稿时间： | 2006-11-01 |
Development of a real-time PCR method for Thalassiosira rotula rapid detection |
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| HE Shanying,YU Zhigang,MI Tiezhu.Development of a real-time PCR method for Thalassiosira rotula rapid detection[J].Acta Oceanologica Sinica,2007,26(2):133-139. |
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| Authors: | HE Shanying YU Zhigang and MI Tiezhu |
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| Affiliation: | 1.College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China2.College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. |
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| Keywords: | red tide Thalassiosira rotula fluorescent quantitative PCR 18S rDNA |
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