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Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara
作者姓名:QIN Yingxue  WANG Jun  SU Yongquan  WANG Dexiang  CHEN Xinzhong
作者单位:QIN Yingxue1,2,WANG Jun1,SU Yongquan1*,WANG Dexiang1,CHEN Xinzhong1,31. Department of Oceanography,Institute of Subtropic Ocean,Xiamen University,Xiamen 361005,China 2. Fisheries College,Jimei University,Xiamen 361021,China 3. Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen 361012,China
基金项目:This study was supported by the Department of Science and Technology of Fujian Province of China under contract No. 2002N009,
摘    要:1 Introduction Groupers are commercially valuable species which attract considerable mariculture interest in Southeast Asia, especially in China’s Mainland, Tai- wan Province, Hong Kong District, and Singapore. In the recent years, groupers have become t…

关 键 词:弧菌  溃疡病  病原细菌  生物化学  海水
收稿时间:2005-09-03
修稿时间:2005-11-28

Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara
QIN Yingxue,WANG Jun,SU Yongquan,WANG Dexiang,CHEN Xinzhong.Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara[J].Acta Oceanologica Sinica,2006,25(1):154-159.
Authors:QIN Yingxue  WANG Jun  SU Yongquan  WANG Dexiang and CHEN Xinzhong
Affiliation:1.Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China;Fisheries College, Jimei University, Xiamen 361021, China2.Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China3.Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China;Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China
Abstract:Studies were conducted to determine the cause of the acute mortality of cage-cultured Epinephelus awoara in the Tong'an Bay of Xiamen, China during the summer of 2002. Predominant bacteria strain TS-628 was isolated from the diseased grouper. The virulence test confirmed that TS-628 was the pathogenic bacterium. Biochemical characteristics of the isolates were determined using the automatic bacterial identification system and standard tube tests. To further confirm the identification, a 1 121 bp 16S rRNA gene sequence of the isolate was amplified by PCR, which had been deposited into Genbank (accession number: AY747308). According to the biochemical characteristics and by comparing the 16S rRNA gene homology of the isolate, the pathogenic bacterium was identified as Vibrio harveyi. Drug sensitivity tests showed that this pathogenic bacterium was sensitive to 16 antibacterials, especially to chloramphenicol and actinospectacin, but completely resistant to antibacterials likes vancomycin, penicillin, lincomycin, and so on.
Keywords:Vibrio harveyi  Epinephelus awoara  Ulcer disease  
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