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| A MICRO-FLUORESCENT METHOD FOR THE DE TERMINATION OF FREE AMINO ACIDS IN SEA-WATER AND THE DISTRIBUTION OF TOTAL FREE AMINO ACIDS | |
| 作者姓名： | 宁修仁 胡钦贤 朱碧英 刘子琳 杨万伦 |
| 作者单位： | Second Institute of Oceanography，National Bureau of Oceanography，Hangzhou，Second Institute of Oceanography，National Bureau of Oceanography，Hangzhou，Second Institute of Oceanography，National Bureau of Oceanography，Hangzhou，Second Institute of Oceanography，National Bureau of Oceanography，Hangzhou，Zhejiang Institute of Provisions Science，Hangzhou |
| 摘 要： | O-phthaldialdehyde is used for the determination of free amino acid in seawater by measuring the fluorescence produced by its reaction with the a-amino group of amino acids. This method is so sensitive that as little as 1-10 nM of amino acids and less than 1 ml of seawater may be sufficient for one test.This method is simple, rapid and accurate and a-amino acids contained in seawater can directly be determined without concentration and extraction. It is free from the interference of large content of inorganic salts and other nitrogenous compounds in seawater.A hundred and fifty-two samples collected at 24 stations in offshore of Zhejiang have been analyzed. The results obtained show that the range of concentration of the total free amino acid is 0.37-4.70 μM. The values are high near shore and low offshore. |
| 收稿时间： | 1983/11/29 0:00:00 |
| 修稿时间： | 1984/1/30 0:00:00 |
A MICRO-FLUORESCENT METHOD FOR THE DE TERMINATION OF FREE AMINO ACIDS IN SEA-WATER AND THE DISTRIBUTION OF TOTAL FREE AMINO ACIDS |
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| MING XIUREN,HU QINXIAN,ZHU BIYING,LIU ZILIN and YANG WANLUN.A MICRO-FLUORESCENT METHOD FOR THE DE TERMINATION OF FREE AMINO ACIDS IN SEA-WATER AND THE DISTRIBUTION OF TOTAL FREE AMINO ACIDS[J].Acta Oceanologica Sinica,1985,4(1):121-130. | |
| Authors: | MING XIUREN HU QINXIAN ZHU BIYING LIU ZILIN and YANG WANLUN |
| Affiliation: | 1.Second Institute of Oceanography, National Bureau of Oceanography, Hangzhou2.Zhejiang Institute of Provisions Science, Hangzhou |
| Abstract: | O-phthaldialdehyde is used for the determination of free amino acid in seawater by measuring the fluorescence produced by its reaction with the a-amino group of amino acids. This method is so sensitive that as little as 1-10 nM of amino acids and less than 1 ml of seawater may be sufficient for one test. This method is simple, rapid and accurate and a-amino acids contained in seawater can directly be determined without concentration and extraction. It is free from the interference of large content of inorganic salts and other nitrogenous compounds in seawater. A hundred and fifty-two samples collected at 24 stations in offshore of Zhejiang have been analyzed. The results obtained show that the range of concentration of the total free amino acid is 0.37-4.70 μM. The values are high near shore and low offshore. |
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