| 台风过境对养殖三疣梭子蟹抗氧化系统及Na+,K+-ATP酶活性的影响 |
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| 引用本文： | 陈夏月,任永宽,李连君,母昌考,李荣华,宋微微,王春琳.台风过境对养殖三疣梭子蟹抗氧化系统及Na+,K+-ATP酶活性的影响[J].海洋学研究,2018,36(3):101-106. |
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| 作者姓名： | 陈夏月 任永宽 李连君 母昌考 李荣华 宋微微 王春琳 |
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| 作者单位： | 宁波大学 海洋学院,浙江 宁波 315211 |
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| 基金项目： | 国家自然科学基金项目资助(41376150) |
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| 摘 要： | 为了研究台风过境对养殖三疣梭子蟹的影响,于2015年7月采集“灿鸿”台风经过象山前后12 h内象山三疣梭子蟹养殖场样品,检测台风前后三疣梭子蟹中T-SOD、CAT、ACP、Na+,K+-ATP酶活性以及MDA、GSH含量的变化。结果显示:台风过后鳃中T-SOD酶活性升高极其显著(p<0.01);CAT酶活性显著升高(p<0.05);MDA和GSH含量则呈现增加趋势,但差异不显著(p>0.05)。肝胰腺中T-SOD酶活性呈现降低趋势,但差异不显著(p>0.05);CAT酶活性呈现升高趋势,差异不显著(p>0.05);MDA含量显著增加(p<0.05);GSH含量增加极其显著(p<0.01)。心脏中T-SOD、CAT酶活性和GSH含量呈现增加趋势而MDA含量呈现降低趋势且差异均不显著(p>0.05)。心脏和鳃中ACP酶活性呈现上升趋势,肝胰腺中ACP酶活性降低,但差异都不显著(p>0.05)。Na+,K+-ATP酶活性在鳃中极显著下降(p<0.01),在肝胰腺中酶活性极显著上升(p<0.01),在心脏中酶活性显著上升(p<0.05)。结果表明台风会造成三疣梭子蟹短期内应激反应,如若不尽快采取措施,后期会对三疣梭子蟹造成较大的损伤。
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| 关 键 词： | 养殖三疣梭子蟹 台风 酶活性 |
| 收稿时间： | 2017-05-22 |
Effect of typhoon on the antioxidant system and Na+, K+-ATPase activity in Portunus trituberculatus |
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| CHEN Xia-yue,REN Yong-kuan,LI Lian-jun,MU Chang-kao,LI Rong-hua,SONG Wei-wei,WANG Chun-lin.Effect of typhoon on the antioxidant system and Na+, K+-ATPase activity in Portunus trituberculatus[J].Journal of Marine Sciences,2018,36(3):101-106. |
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| Authors: | CHEN Xia-yue REN Yong-kuan LI Lian-jun MU Chang-kao LI Rong-hua SONG Wei-wei WANG Chun-lin |
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| Affiliation: | School of Marine Sciences,Ningbo University, Ningbo 315211, China |
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| Abstract: | In order to study the effect of typhoon on the cultured Portunus trituberculatus, the changes of T-SOD, CAT, MDA, GSH, ACP and Na+, K+-ATPase in the hearts, gills and hepatopancreas were detected in 12 hours before and after typhoon “Canhong”(1509) at Xiangshan in July, 2015. The typhoon increased highly significantly the activity of the T-SOD enzyme (p<0.01) and increased significantly CAT enzyme(p<0.05) in the gills. After typhoon, the MDA and GSH content in gills increased insignificantly (p>0.05). T-SOD enzyme activity in the hepatopancreas showed an insignificant trend of decrease (p>0.05) while the CAT enzyme activity showed an insignificant trend of increase (p>0.05), MDA content increased significantly (p<0.05) and the increase of the GSH content was highly significant (p<0.01). In the hearts, the levels of T-SOD, CAT enzyme activity and GSH content increased and the MDA content decreased, but these changes were not significant(p>0.05). In addition, the ACP enzyme activity in the gills and the hearts increased insignificantly(p>0.05), which was reduced but not significantly in the hepatopancreas (p>0.05). The Na+, K+-ATPase activity in the gills decreased highly significantly (p<0.01), but increased highly significantly in the hepatopancreas (p<0.01) and significantly in the heart (p<0.05) . This study reveals that the typhoon can cause the cultured P. trituberculatus stress response in the short term and solutions are need to take to avoid typhoon damage to cultured P. trituberculatus. |
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| Keywords: | cultured Portunus trituberculatus typhoon enzyme activity |
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