| 斑节对虾病原菌胞外产物的致病性研究 |
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| 引用本文： | 张朝霞,王军,苏永全,幺久刚,周胜利,鄢庆枇.斑节对虾病原菌胞外产物的致病性研究[J].海洋学报,2000,22(5):94-99. |
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| 作者姓名： | 张朝霞 王军 苏永全 幺久刚 周胜利 鄢庆枇 |
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| 作者单位： | 厦门大学海洋学系, 亚热带海洋研究所, 厦门 361005 |
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| 基金项目： | 国家高技术研究发展计划专题”高健康斑节对虾精养管理与示范”资助项目(编号：863－819－02－05)． |
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| 摘 要： | 由养殖濒死病虾体内分离的副溶血弧菌(2019#)、溶藻弧菌(4302#)和嗜水气单胞菌(1003#)培养液中分别提取了胞外产物,测定了酶成分,定量分析了蛋白酶及磷酸酯酶的活性.用细菌胞外产物粗提液对斑节对虾进行致死试验,戊二醛固定致死对虾的肝组织,电镜观察了斑节对虾肝组织的超微病变,初步探讨了三株细菌胞外产物诱发虾病的病理.
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| 关 键 词： | 病原菌 胞外产物 斑节对虾 |
| 收稿时间： | 1999/3/16 0:00:00 |
| 修稿时间： | 1999-03-16 |
Studies on pathogenesis of extracellular products from pathogenetic bacteria in Penaeus monodon Fabrivius |
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| Zhang Zhaoxi,Wang Jun,Su Yongquan,Yao Jiugang,Zhou Shengli and Yan Qingpi.Studies on pathogenesis of extracellular products from pathogenetic bacteria in Penaeus monodon Fabrivius[J].Acta Oceanologica Sinica (in Chinese),2000,22(5):94-99. |
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| Authors: | Zhang Zhaoxi Wang Jun Su Yongquan Yao Jiugang Zhou Shengli and Yan Qingpi |
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| Affiliation: | Departmeat of Oceanography, Subtropical Institute of Oceanography, Xiamen University, Xiamen 361005 |
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| Abstract: | The extracellular products of Vibrio parahaemolyticus, V. alginolyticus and Aeromonas hydrophila, which were isolated from diseased Penaeus monodon in Tong an from June to August 1997, are extracted and investigated. Composition of extracellular enzymes from these three bacteria is detected. Among them, protease and phospholiase, including both acidic phospholiase and alkine phospholiase, are chosen to do quantitative analysis on their activities. These three kinds of the above enzymes extracted from cultures of V. parahaemolyticus are more active than those of the others. Bacterial extracellular products are used to do lethal experiments by injecting to the healthy P. monodon. Conforming to the result of activity of protease and phospholiase, lethal action of V. parahaemolyticus is the strongest and that of V. alginolyticus is the weakest. The pathological variations of dying shrimps livers in the lethal experiments are observed by transmit electron microscope (TEM). Cells of those shrimps livers show to be destroyed at different extents, for example, nuclear core of some cells disappeared and nuclear membrane are wrecked, mitochondria are destroyed, even the whole cell is disintegrated. It is showed that not only dxtracellular products of these three strains are poisonous and could be mortal to shrimps, but also the activities of protease and phospholiase in extracellular products of pathogen are related to toxicity of these three strains. |
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| Keywords: | Pathogens extracellular products Penaeus monodon |
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