| 文昌鱼鳃组织原代培养 |
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| 引用本文： | 石松林,黄晓明,李祺福,王三英,杨海波.文昌鱼鳃组织原代培养[J].海洋科学,2009,33(3):44-48. |
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| 作者姓名： | 石松林 黄晓明 李祺福 王三英 杨海波 |
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| 作者单位： | 厦门大学,生命科学学院,福建,厦门,361005 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用组织块培养法培养文昌鱼(Branchiostoma belcheri japonicum)鳃细胞,使用L15,M199,Eargle'sMEM为基础培养基,加入一定量的NaCl及5%,10%,20%等不同体积分数的牛血清.经条件优化后得到最适合的培养条件为2L15+20%FBS+0.45%NaCl,在26～28℃条件下培养,组织块贴壁后12 h内就有至少4类上皮样细胞从中迁移出来,这些细胞在前3天迅速增殖,到第6天基本上达到平稳期,往后细胞开始凋亡,最长可以存活近两个星期.鳃细胞的原代培养为文昌鱼细胞体外培养的深入研究提供了实验依据.
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| 关 键 词： | 文昌鱼(Branchiostoma belcheri japonicum) 鳃组织 原代培养 |
| 收稿时间： | 2006/11/13 0:00:00 |
| 修稿时间： | 1/5/2009 12:00:00 AM |
Primary cell culture from gill explants of amphioxus |
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| SHI Song-lin,HUANG Xiao-ming,LI Qi-fu,WANG San-ying,YANG Hai-bo.Primary cell culture from gill explants of amphioxus[J].Marine Sciences,2009,33(3):44-48. |
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| Authors: | SHI Song-lin HUANG Xiao-ming LI Qi-fu WANG San-ying YANG Hai-bo |
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| Affiliation: | College of Life Science;Xiamen University;Xiamen 361005;China |
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| Abstract: | Primary cultures of gill cells were initiated from gill filament explants of amphioxus(Branchiostoma belcheri japonicum) by a tissue block culture method.The explants were cultured in Leibovitz L15 medium,M199,Eargle's MEM medium with 5%,10% or 20% foetal calf serum(FCS) and a certain amount of NaCl.We found that gill cell could grow better in 2 L15 medium than in others.The attachment efficiency was serum-dependent though increased FCS concentration did not stimulate further outgrowth of cells.The explants... |
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| Keywords: | Branchiostoma belcheri japonicum gill cell primary cell culture |
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