| 羊栖菜养殖品系DNA指纹图谱的研究 |
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| 引用本文： | 吕慧,姚建亭,刘福利,王秀良,李生尧,段德麟.羊栖菜养殖品系DNA指纹图谱的研究[J].海洋科学,2009,33(12):30-33. |
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| 作者姓名： | 吕慧 姚建亭 刘福利 王秀良 李生尧 段德麟 |
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| 作者单位： | 1. 中国科学院海洋研究所,山东,青岛,266071
2. 洞头县鹿丰羊栖菜研究所,浙江,温州,325700 |
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| 基金项目： | 国家自然基金项目，洞头县科技计划项目 |
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| 摘 要： | 对羊栖菜(Hizikia fusiformis (Harv) Okamura)养殖中常见的3个品系进行了DNA指纹分析及遗传变异的研究,构建了其遗传指纹图谱,分析了不同种群的遗传关系,为羊栖菜的种质鉴定及选育提供了理论依据.运用RAPD分子标记技术,对5个羊栖菜的种群中共125个个体进行了分析,从300个引物中筛选出12条随机扩增引物共扩增135个位点,多态位点比率为84.4%.从中选择了4个多态性位点,构建了DNA指纹图谱.相关结果对羊栖菜遗传选育和种质鉴定等有参考价值.
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| 关 键 词： | 羊栖菜(Hizikia fusiformis (Harv) Okamura) DNA指纹图谱 种群遗传分析 |
| 收稿时间： | 6/5/2009 12:00:00 AM |
| 修稿时间： | 2009/9/10 0:00:00 |
Construction of DNA fingerprint for Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta) strains based on RAPD analysis |
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| Lü Hui,YAO Jian-ting,LIU Fu-li,WANG Xiu-liang,LI Sheng-yao,DUAN De-lin.Construction of DNA fingerprint for Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta) strains based on RAPD analysis[J].Marine Sciences,2009,33(12):30-33. |
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| Authors: | Lü Hui YAO Jian-ting LIU Fu-li WANG Xiu-liang LI Sheng-yao DUAN De-lin |
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| Abstract: | In this study, we analyzed the genetic distances and constructed the fingerprints of the three good strains, which have been widely applied in the production. It was indicated that: RAPD (random amplified polymorphic DNA) was applied to analyzing 125 individuals from the 5 populations of Hizikia. A total of 135 amplification loci were obtained from 12 selected primers after screening and P% (the percentage of polymorphic loci) is 84.4%. The yielded 4 stable and clear bands were selected to construct a fingerprint for discrimination of the 5 populations. Our results provide the tools for the Hizikia's genetic breeding and germplasm identification in the future. |
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| Keywords: | RAPD Hizikia fusiformis DNA fingerprint genetic analysis Random amplified polymorphic DNA (RAPD) |
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