| 刺参肌肉组织cDNA文库的构建 |
| |
| 引用本文： | 陈璐,姜国良,刘云,仇磊,项鹏.刺参肌肉组织cDNA文库的构建[J].海洋科学,2009,33(12):1-3. |
| |
| 作者姓名： | 陈璐 姜国良 刘云 仇磊 项鹏 |
| |
| 作者单位： | 中国海洋大学,生命科学与技术学部,山东,青岛,266003 |
| |
| 基金项目： | 国家自然科学基金项目 |
| |
| 摘 要： | 用RNA提取试剂--TRIZOL Reagent提取刺参(Apostichopus japonicus)肌肉组织总RNA,用SMART cDNA Library Construction Kit构建cDNA文库.经测定原始文库滴度达到 3.2×10~6,扩增后文库滴度达到 5.1×10~9,重组率达到96.7%,从扩增文库随机挑取12 个克隆进行PCR 扩增鉴定,结果显示,插入片段大小为0.5～2.5 kb.通过各项指标验证成功构建了刺参肌肉组织cDNA 文库.
|
| 关 键 词： | 刺参(Apostichopus japonicus) 肌肉组织 cDNA文库 |
| 收稿时间： | 2006/5/15 0:00:00 |
| 修稿时间： | 2009/9/16 0:00:00 |
Construction of cDNA library from the musculature of Apostichopus japonicus |
| |
| CHEN Lu,JIANG Guo-liang,LIU Yun,QIU Lei and XIANG Peng.Construction of cDNA library from the musculature of Apostichopus japonicus[J].Marine Sciences,2009,33(12):1-3. |
| |
| Authors: | CHEN Lu JIANG Guo-liang LIU Yun QIU Lei and XIANG Peng |
| |
| Affiliation: | CHEN Lu,JIANG Guo-liang,LIU Yun,QIU Lei,XIANG Peng(Faculty of Life Science and Technology,Ocean University of China,Qingdao 266003,China) |
| |
| Abstract: | Total RNA of muscles of Apostichopus japonicus were extracted by TRIZOL Reagent. cDNA library was constructed with SMART cDNA library construction kit. The primary library hada high titer of 3.2×10~6, in which 96.7% clones were recombinant. The amplified library hada titer of 5.1×10~9, and the length of most cDNA inserted in the library was about 0.5～2.5 kb. It was concluded that the cDNA library of Musculature of A. japonicus had been constructed by using the SMART technique. |
| |
| Keywords: | Apostichopus japonicus muscle cDNA library |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋科学》浏览原始摘要信息 |
|
点击此处可从《海洋科学》下载全文 |
|
