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1.
The lipid fraction of particulate organic matter in seawater was analyzed by gravimetry, liquid chromatography, gas chromatography (GS), and gas chromatography—mass spectrometry (GS—MS). Gravimetric concentrations in the Gulf of Mexico were in the range of 12–70 μg 1?1 for near-surface samples and 9–52 μg 1?1 for near-bottom samples. The lipids accounted for ～20% of the particulate organic carbon. Particulate lipids were found to be a much more complex mixture of organic compounds than the dissolved lipids and their composition was much more variable. The majority of the extracted weight was recovered in the polar liquid chromatography fraction (49–85%). The major components of the analyzable particulate lipids were similar to those of the dissolved lipids, namely, n-alkanes, pristane, phytane, and methyl, ethyl and propyl fatty acid esters. Often olefins, alkylated benzenes, quinones, and the unresolved GC area contribute a substantial part to the total particulate lipids. Minor components included ketones, phenols, indanes, acids, benzoates, aromatics and possibly derivatives of inositol, dioxane, and pyran. The low concentrations observed confirm that the area is relatively pristine though some evidence for chronic low-level oil pollution was present. Seasonal, diurnal, vertical and areal variations were observed in the particulate lipids. Lipid concentrations not only decreased with depth but they were also a decreased percentage of the organic carbon present. This may indicate the loss of labile carbon with depth due to reworking in the euphotic zone. Anoxic conditions appeared to promote the preservation of lipid materials in the particulate phase, n-Alkanes and fatty acid esters were common to both the particulate and dissolved phases and had similar distributions.  相似文献   

2.
Volatile fatty acid (VFA) apparent turnover rates in organic-rich marine sediments were determined by measuring whole sediment VFA concentration and the corresponding first-order reaction rate constants. In order to measure VFA concentrations, bulk wet sediment samples were basified, freeze-dried, extracted with methanol, derivatized to form methyl esters of the VFAs, and analyzed by packed-column gas chromatography using hexanoic acid as an internal standard. The detection limits for acetate, propionate, iso-butyrate and butyrate were 1.0, 0.4, 0.2 and 0.2 μmol l?1s, respectively, for 600 ml samples. Rate constants for acetate and propionate were determined by anaerobically incubating samples at in-situ temperatures with tracer levels of 14C-labelled VFAs. Metabolized label was recovered as CO2, CH4, cellular material, water-soluble material, and VFA (ether-soluble) fractions. Apparent turnover rates measured during summer and winter in anoxic Cape Lookout Bight, North Carolina (U.S.A.) sediments were in the range of 19–330 μmol l?1s h?1 for acetate and 0.7–7.0 μmol l?1s h?1 for propionate.  相似文献   

3.
The vertical distribution of dissolved aluminium in the Mediterranean Sea offshore of Corsica has been followed during a period of high biological activity (April–September). In May and August, the concentration of dissolved aluminium is at a relatively low (1.5 μg Al1?1) and rather constant value in the surface waters, while exhibiting minimum values of about 0.5 and 0.8 μg Al1?1 in April and September. It increases under the seasonal thermocline to a depth of 150–500 m. The bottom waters are characterized by a constant and relatively high value of 4 μg Al1?1.The observed concentrations of dissolved aluminium are not compatible with a precipitation-dissolution mechanism of clay minerals.The general distribution pattern and the seasonal changes of dissolved aluminium exhibit strong analogies with those of dissolved silica and nitrogen, suggesting a pre-dominant role of biological activity. It is not possible at this stage to attribute the aluminium uptake in the photic zone solely to the activity of diatoms, as suggested earlier.  相似文献   

4.
Abstract

Chemical parameters (pH, Eh, carbon, Kjeldahl nitrogen, total phosphorus, 0.5M H2SO4‐extractable phosphorus, organic phosphorus, and water‐soluble phosphorus) were measured in the surface layers of sediments collected from various depths in Lakes Rotowhero, Okaro, Ngapouri, Rotokakahi, Okareka, Tikitapu, Okataina, and. Rotoma during October 1972. The sediments of the productive geothermal lake, Rotowhero, were markedly different from those of the cold‐water lakes: they had relatively low pH values, high carbon (mean 8.5%) and organic phosphorus (mean 4160 μg.g?1) concentrations, and very high total phosphorus concentrations (mean 4770 μg.g?1), probably as a result of enrichment by hot springs.

The mean concentrations in the sediments of the cold‐water lakes were carbon 3.2–7.9%, Kjeldahl nitrogen 3380–8310 μg.g?1 and phosphorus 690–1780 μg.g?1. These concentrations are within the ranges for New Zealand terrestrial topsoils, but the lake sediments appear enriched in phosphorus relative to local topsoils. Total carbon, nitrogen, and phosphorus concentrations of sediments tended to be highest in the eutrophic lakes (Okaro, Ngapouri) although the deep oligotrophic lakes (Okataina, Rotoma) had relatively high total phosphorus concentrations (means 1400, 1510 μg.g?1). Overall, the carbon, nitrogen, and phosphorus concentrations of the sediments showed little relationship to the trophic state of the lake.

Organic phosphorus concentrations of the surface layers of sediments were similar in all the cold‐water lakes (mean 319 μg.g?1). The proportion of the total phosphorus apparently ‘fixed’ in mineral material was minimal (0–1%) in sediments from the eutrophic and mesotrophic lakes, but in the oligotrophic lakes was similar to that in New Zealand topsoils (9–14%). Reducing conditions may cause solution of a high proportion of the ‘fixed’ phosphorus in the eutrophic lakes.

The water‐soluble phosphorus concentrations in the sediments of the five shallow cold‐water lakes (Okaro, Ngapouri, Rotokakahi, Okareka, Tikitapu) correlated positively with trophic state and with concentrations of dissolved phosphorus in the lake waters.

Carbon, nitrogen, and phosphorus concentrations in the sediments tended to vary with overlying water depth. This should be considered when comparisons are made between lakes.  相似文献   

5.
Concentrations of dissolved methane in seawater and bottom sediments, as well as of methane emanating from gas seeps were measured at 18 stations including several small bays in the Sevastopol coastal area (Black Sea) during 2007–2008. Methane concentrations in surface waters ranged from 10 to 2,970 nmol l?1, and correlated well with values recorded for sediments. Methane concentrations in the water column were influenced by water depth, as well as by air and water temperatures. In the spring and summer of 2008, in situ CH4 saturation relative to air was in the range of 970–71,900%. Maximum saturation was in summer. CH4 fluxes to the atmosphere from the Sevastopol coastal area were estimated to vary from 190 to 1,550 μmol m?2 day?1. Gas bubbles escaping from the seepages contained about 57 vol% methane. Radiocarbon dating of the methane revealed an age not exceeding 150 years, implying a biogenic origin.  相似文献   

6.
A total of 150 samples were collected at a 10-days' anchor station in the Bornholm basin (55° 31.1′N, 15° 32.1′E) and analyzed for dissolved (< 0.4 μm) and particulate trace metals. For dissolved Mn, large gradients have been found in the vertical distribution with minimum concentrations (< 0.2 μgl?1) in the halocline zone and considerably higher values in the deep waters (up to 50 μgl?1). Ultrafiltration studies indicate that dissolved Mn is probably present as Mn2+ in the oxygenated bottom layer. The primary production process was not evident in the particulate Mn profile; the suspended particulate material (SPM), however, shows a considerable enrichment with depth, apparently due to Mn-oxide precipitation.The distribution of dissolved Fe was rather homogeneous, with average concentrations throughout the water column between 0.86 and 1.1 μgl?1, indicating that the oxidation of Fe2+ ions released from the sediments must already be complete in the very near oxidation boundary layer. Relatively high concentrations of particulate Fe were actually measured in the bottom layer, with the maximum mean of 11.2 μgl?1 at 72 m. Similarly to Mn, the profile of particulate Fe does not reflect the SPM curve of the eutrophic layer. On average, about 70% of the total Fe in surface waters was found to be particulate.The average concentrations of dissolved Zn, Cd and Cu were found to be rather homogeneous in the water column but showed a relatively high variability with time. A simplified model on trace-metal uptake by phytoplankton indicates no significant change in dissolved metal concentrations during the period of investigation. On average, only 1.7% Zn, 3.3% Cd and 9.8% Cu of the total metal concentrations were found in particulate form. SPM analyses showed significant correlations of Zn, Cd and Cu with Fe, indicating that particulate iron is an important carrier for particulate trace metals in Baltic waters.  相似文献   

7.
A relatively precise and rapid method for the analysis of total dissolved monosaccharides at the concentrations that occur in seawater is described which uses 1-ml quantities for each analytical and control replicate. The alditols remain unchanged while the pentoses and hexoses are reduced to the alditol form by borohydride. The total alditols are then oxidized with periodate to form two moles of formaldehyde per mole of monosaccharide and the formaldehyde is determined spectrophotometrically with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH). Unlike other spectrophotometric methods, similar curves are obtained for equimolar concentrations of different carbohydrates while the differences on a weight basis are only due to the small difference in molecular weight between pentoses and hexoses. Winter—Spring samples from surface waters of lower Narragansett Bay ranged from 122 to 226 μg l?1 with a mean of 159 μg l?1.  相似文献   

8.
The concentrations of the trace metals Cd, Cu, Fe, Ni, Pb and Zn in the Göta River estuary have been investigated. The following metal fractions have been determined: acid-leachable, dissolved, labile and particulate.The estuary represents a salt wedge type estuary and is situated in a densely populated region of Sweden. The metal concentrations found for the dissolved fraction is in the range of what can be considered as background levels for freshwater. It is difficult to evaluate any estuarine processes other than conservative mixing for Cd, Cu, Ni and Zn. The dissolved levels in the freshwater end member are Cd, 9–25 ngl?1; Cu, 1·1–1·4 μgl?1; Fe, 20–75 μg l?1: Ni, 0·7–0·9 μg l?1: Pb 0·09–0·2 μg l?1; and Zn, 6–7 μg l?1:The results from the acid-leachable fraction show that at high suspended load the particles sediment in the river mouth. The trace metal levels in this fraction are subject to large variations.  相似文献   

9.
Progress in the introduction of coulometry for the analysis of total carbon dioxide (TCO2) in marine waters is described. An extractor—stripper removes CO2 that is measured coulometrically by the quantity of electricity (coulombs) used to electrogenerate OH? ions for the titration of the acid formed by the reaction of CO2 and ethanolamine. The equivalence point is detected photometrically with thymolphthalein as the indicator, and Faraday's Law relates coulombs to equivalents of titrant generated and CO2 determined so that there are no standard curves needed or titrants to standardize or store. Accuracy was determined by adding gelatin capsules containing 100–1500 μg C of pure CaCO3 into the stripper, and accuracies of better than ± 1 μg C were achieved. The best precision for natural seawater (± 1 standard error) of ± 0.5 μmol l?1 was found for 17 samples of Bermuda coastal waters having a mean TCO2 of 2007.2 μmol l?1 (0.05% CV). Sources of error and precautions are discussed. This method, which has been used successfully at sea, can be used to study a variety of marine phenomena involving TCO2.  相似文献   

10.
Cd, Cu, Fe, Ni, Pb and Zn were determined in 123 samples from the Baltic Sea proper. The trace metals were extracted directly on board the vessel, using a dithiocarbamate-Freon procedure. Final analyses of the extracts are performed onshore by atomic absorption spectrometry.Similar trace-metal concentrations are found in different areas of the Baltic proper. Most values fall in the following ranges: Cd, 30–60 ng 1?1; Cu, 0.6–1.0 μg 1?1; Fe, 0.3–0.9 μg 1?1; Ni, 0.6–0.9 μg 1?1; Pb, 0.05–0.2 μg 1?1; and Zn, 1.5–3.5 μg 1?1. The metal-concentrations are generally independent of depth. However, copper exhibits a small but significent decrease in concentration below 80 m.Filtration did not affect trace-metal concentrations, with the exception of iron in waters from lower layers. Similarly, storage under acid conditions was shown to affect only the concentration of iron. An electro-chemical technique was also used to determine Cu in some samples.  相似文献   

11.
Dissolved and total dissolvable manganese concentrations have been measured at four stations in the western North Atlantic Ocean. Total dissolvable manganese concentrations are high in surface waters, decrease to uniformly low levels throughout the bulk of the water column, and increase in the bottom nepheloid layer. Dissolved Mn (Mnd) concentrations follow the total dissolvable concentrations throughout the surface and deep waters but do not increase in the near-bottom waters.Deep water concentrations of Mnd decrease from 30 ng l?1 in the Newfoundland Basin to 20 ng l?1 in the Sargasso Sea. This change and other features of the deep water distribution of dissolved manganese could be associated with the slow oxidation of Mn2+ to MnO2. There is also evidence at one station of scavenging of manganese from the dissolved phase in the near-bottom layer which may again be related to the kinetics of manganese oxidation.  相似文献   

12.
A procedure is described which adds a hydrolysis step to the Johnson and Sieburth 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) assay for total dissolved monosaccharides. The advantages of the monosaccharide test are retained in the total carbohydrate test, giving nearly equivalent responses for a variety of combined carbohydrates. A total sample of 50 ml is adequate for both total carbohydrate and monosaccharide assays which permit the estimation of polysaccharide by difference. Values for Narragansett Bay and adjacent waters ranged from 452 to 2023 μg l?1 for total dissolved carbohydrate, 272 to 1353 μg l?1 for polysaccharide, and 153 to 814 μg l?1 for monosaccharide, which accounted for 6–18%, 4–13%, and 2–5% of the total dissolved carbon, respectively. We suggest that this is a sensitive and precise procedure which will be useful for investigating the distribution of dissolved carbohydrates in seawater and factors which affect its production, distribution and utilization.  相似文献   

13.
W. Balzer 《Marine Chemistry》1985,15(4):379-380
Representative profiles of inorganic nitrogenous species dissolved in interstitial waters of coral reef sands are presented. Ammonium is the dominant nitrogenous species in these pore waters with concentrations of up to 40 μm. Nitrate is present but in lower concentrations. Nitrite is found only occasionally in trace amounts. Computations of diffusive fluxes and inferences concerning microbial activity are derived from the profile structures. Computed flux rates of nitrogenous species from the sediment to the water column range between 0.75 and 1.37 μM m?1 h?1. These inputs may represent a significant source of recycled nitrogen to the primary producers of the coral reef ecosystem.  相似文献   

14.
In the coastal and estuarine waters of Goa, particulate organic carbon (POC) varied from 0.52 to 2.51 mg l?1 and from 0.28 to 5.24 mg l?1 and particulate phosphorus (PP) varied from 0.71 to 5.18 μg l?1 and from 0.78 to 20.34 μg l?1, respectively. The mean values of chlorophyll and primary productivity were 1.94 mg m?3 and 938.1 mg C m?2 day?1 in the coastal waters and 4.3 mg m?3 and 636.5 mg C m?1 day?1 in the estuarine waters, respectively.POCchl ratios were low in June and October even when POC values were quite high. The POC in surface waters was linearly correlated with the chlorophyll content. Also PP increased when chlorophyll and primary productivity remained high. The results suggest that the phytoplankton was sharply increasing and contributed to POC and PP content. The percentage of detritus calculated from the intercept values of chlorophyll on POC varied from 46 to 76% depending on season. Results indicate that the major portion of POC and PP during postmonsoon (October–January) is derived from phytoplankton production while the allochthonous matter predominate during monsoon (June–September).  相似文献   

15.
Capillary gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) have been used to examine the aliphatic hydrocarbon fraction of specimens of the mussel, Mytilus edulis, collected from the legs of a North Sea oil-production platform. In addition to an unresolved complex mixture (UCM) of fossil fuel pollutant hydrocarbons (ca. 64 μg g?1 dry weight average), the fraction was dominated by large amounts (ca. 21 μgg?1 dry weight) of C31 and C33n-alkenes with 2, 3 and 4 double bonds. These alkenes have also been identified in the ubiquitous marine alga, Emiliania huxleyi, and in some species of fish taken from the North Sea. The close correspondence in the composition data suggest that, in the mussel, these alkenes arise from a biogenic dietary source, most probably from Emiliania huxleyi. This contention is supported by the identification in the mussel of unusual C37, C38 and C39 unsaturated straight-chain ketones which have recently been shown to be biosynthesised by Emiliania.  相似文献   

16.
The geochemistry of dissolved copper-organic complexes was investigated in the estuarine waters of Narragansett Bay. A transect survey was conducted in August 1980, while one mid-bay station was monitored from March through August of that year. The transect data indicated that most of the copper-organic complexes enter the bay via sewage effluent which is discharged into the Providence River at the head of the bay. Organic copper concentrations in the estuary ranged from 0.12 to 2.30 μg kg?1 and comprised from 14 to 70% of the total dissolved copper. The concentration of copper-organic complexes was not directly related to the amount of dissolved organic matter; and recently generated organic material from phytoplankton production within the bay had a negligible influence on the fraction of dissolved copper which was organically bound.The major source of total copper to the bay is anthropogenic inputs from sewage effluents. Particulate and dissolved copper concentrations ranged from 0.06 to 2.42 and 0.23 to 16.4 μg kg?1, respectively, giving average values of about 40% particulate and 60% dissolved copper. Particulate copper concentrations decreased rapidly from the upper to the lower bay as a result of both removal and dilution. About 75% of the dissolved copper entering the bay is rapidly removed in the Providence River and upper bay, and the remaining portion (which is largely organic copper) follows conservative mixing in the mid to lower bay. The data suggest that copper binding by dissolved organic matter may be an important control on the riverine flux of dissolved copper through estuaries into coastal and oceanic waters.  相似文献   

17.
Monosaccharides were determined in waters and sediment pore waters from a wide variety of environments and locations. Desalting was performed by electrodialysis and concentrated extracts were analyzed by two forms of liquid chromatography. At least 12 sugars were identified. Glucose and fructose were the dominant monosaccharides in nearly all samples examined. Analyses of over 150 seawater and sediment pore water samples showed that fructose is highly significantly correlated (>0.99) with glucose concentrations. Laboratory kinetic experiments on the epimerization reaction, glucose ? fructose, in sterilized natural seawater in the dark, yielded a forward rate constant, k1, of 1.2 × 10?5 h?1 at 25°C and 1.6 × 10?7 h?1 at 2°C. The approximate time required to attain equilibrium at 25°C was 1.1 y and at 2°C, 15.4 y; the measured equilibrium constant was in the range 0.3–0.4. The actual ratio of fructose to glucose in natural water samples was usually in the range 1.0–1.4/l, independent of the total monosaccharide concentrations, which varied in the samples by a factor of ～100. Several possible explanations for the apparent discrepancy in the determined and found ratios are discussed: the presence of universal, as yet, undiscovered major biological sources for fructose in marine environments; preferential utilization of glucose; preferential incorporation of fructose into or onto inorganic colloids; and preferential formation of fructose-transition metal ion associations.  相似文献   

18.
《Oceanologica Acta》1998,21(2):279-291
During the spring 1995 (2–25 May), a cruise was carried on the RV Poseidon (Germany) on the continental shelf of the south Bay of Biscay. The objective was a comprehensive study of the planktonic food web within the Gironde plume waters. In these waters phosphate was present at very low concentrations (undetectable to < 0.1 μmol.L−1), whereas nitrate, silicate and ammonium concentrations were much higher (several μmol·L−1 for nitrate and silicate and 0.5 to 1.0 μmol·L−1 for ammonium). The size distribution of the phytoplankton biomass (estimated from chlorophyll a measurements by high performance liquid chromatography) and primary production (measured by 14C in situ method) showed a great proportion of small (40 to 70 % < 3 μm) and active autotrophic cells (growth rates estimated from 0.4 to 0.8 d−1 for the entire euphotic layer). Considering the very high values of NO3-N:PO4-P ratios and the high C:P and N:P ratios for the particulate organic matter, it is suggested that an early phosphorus depletion limits the spring bloom phytoplankton and particularly the new production (nitrate uptake coming from the Gironde waters).From these results and other simultaneous observations on the heterotrophic processes (such as grazing of microzooplankton), we can conclude that the planktonic food web would be close to a maintenance system as defined by Platt et al. The possible generalisation of these results for each spring is discussed with respect to the scarcity of previous and reliable phosphate data.  相似文献   

19.
The areas that we studied in the North Atlantic (53 and 60°N) and in the Labrador Sea in the summer were characterized by a wide variability of the concentrations of dissolved and particulate organic matter and its elemental composition both in the surface and in the deep waters. The concentrations of dissolved and particulate Corg varied within 69–360 μM and 0.7–25.6 μM, respectively; the Norg and Porg contents varied within 1.4–22.2 μM and 0.02–0.86 μM, respectively. The maximal concentrations were registered in the photic layer and in the zones of mixing between the waters of different genesis. The particulate matter contribution to the total organic matter (OM) content varied from 0.5 to 15.4%. The waters of the photic layer contained more particulate Corg than those of the near-bottom layer. The values of the C/N molar ratios from the surface to the bottom over the entire aquatic area surveyed varied 5-to 6-fold; at that, the values of the C/P molar ratios varied more than tenfold. In the most productive waters, the values of the C/N ratios were close to the Redfield ratios (6–10). The values of the C/P molar ratios varied from 160 in the photic layer to 4831 in the deep waters. The pronounced non-uniformity in the spatial distribution of the OM and its elemental composition is caused not only by the penetration of the waters of different origins but also by the changes in the microplankton metabolism under mixing of these waters.  相似文献   

20.
Hong Kong waters receive high nutrient loading from year-round sewage effluent and Pearl River discharge during the summer wet season. We assessed the role of physical processes in reducing eutrophication by calculating a eutrophication reduction index for four different hydrographical areas and four seasons. We used outdoor incubation experiments to assess the response of phytoplankton when physical (mixing and dilution) processes and mesozooplankton grazing were reduced. The primary regulator of phytoplankton growth in low nutrient eastern waters (reference site) shifted from nutrients in the wet season to increased vertical mixing in the dry season. In the highly flushed western waters and Victoria Harbour, the majority (>86?%) of the eutrophication impacts were reduced by strong hydrodynamic mixing (turbulence, vertical mixing, and flushing effects) all year. In southern waters, eutrophication effects were severe (chlorophyll a of up to ~73?μg?L?1) and was regulated by the ambient phosphate (PO4) concentration (~0.1?μM) during summer. In contrast, 62–96?% of the potential eutrophication impacts were reduced by physical processes during other seasons. Bioassays also revealed that the yield of chlorophyll from dissolved inorganic nitrogen (DIN) that was taken up by phytoplankton [1.1–3.3?g?Chl (mol?N)?1] was not significantly different in both N- and P-limited cases. In contrast, the uptake ratios of DIN:PO4 (26:1–105:1) and Chl:P ratios [42–150?g?Chl (mol?P)?1] in the P-limited cases were significantly (p?<?0.05, t test) higher than the N-limited cases [~16DIN:1P and 22–48?g?Chl (mol?P)?1]. The C:Chl ratios ranged from 32 to 87?g?g?1. These potential ranges in ratios need to be considered in future nutrient models.  相似文献   

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