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1.
The concentrations of suspended matter and particulate Cd, Cu, Pb and Zn were determined for 36 samples collected at 6 stations in the Antarctic Ocean during December, 1970 and January, 1971 using membrane filters. The concentration of suspended matter was determined gravimetrically and trace metal levels were determined using anodic stripping voltammetry. For waters deeper than 100 m the concentration of suspended matter was < 100 μg l?1. Concentrations up to 542 μg l?1 were recorded between surface and 100 m. Individual concentrations of the metals were scattered with depth. Average concentrations of particulate metals were: Cd, 3.5 ng l?1; Cu, 100 ng l?1; Pb, 35 ng l?1; and Zn, 230 ng ;l?1 These measurements represent non-steady state conditions of early Antarctic summer as the ice pack disintegrates and biological activity increases.  相似文献   

2.
The concentrations of the trace metals Cd, Cu, Fe, Ni, Pb and Zn in the Göta River estuary have been investigated. The following metal fractions have been determined: acid-leachable, dissolved, labile and particulate.The estuary represents a salt wedge type estuary and is situated in a densely populated region of Sweden. The metal concentrations found for the dissolved fraction is in the range of what can be considered as background levels for freshwater. It is difficult to evaluate any estuarine processes other than conservative mixing for Cd, Cu, Ni and Zn. The dissolved levels in the freshwater end member are Cd, 9–25 ngl?1; Cu, 1·1–1·4 μgl?1; Fe, 20–75 μg l?1: Ni, 0·7–0·9 μg l?1: Pb 0·09–0·2 μg l?1; and Zn, 6–7 μg l?1:The results from the acid-leachable fraction show that at high suspended load the particles sediment in the river mouth. The trace metal levels in this fraction are subject to large variations.  相似文献   

3.
A procedure is described which adds a hydrolysis step to the Johnson and Sieburth 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) assay for total dissolved monosaccharides. The advantages of the monosaccharide test are retained in the total carbohydrate test, giving nearly equivalent responses for a variety of combined carbohydrates. A total sample of 50 ml is adequate for both total carbohydrate and monosaccharide assays which permit the estimation of polysaccharide by difference. Values for Narragansett Bay and adjacent waters ranged from 452 to 2023 μg l?1 for total dissolved carbohydrate, 272 to 1353 μg l?1 for polysaccharide, and 153 to 814 μg l?1 for monosaccharide, which accounted for 6–18%, 4–13%, and 2–5% of the total dissolved carbon, respectively. We suggest that this is a sensitive and precise procedure which will be useful for investigating the distribution of dissolved carbohydrates in seawater and factors which affect its production, distribution and utilization.  相似文献   

4.
In the coastal and estuarine waters of Goa, particulate organic carbon (POC) varied from 0.52 to 2.51 mg l?1 and from 0.28 to 5.24 mg l?1 and particulate phosphorus (PP) varied from 0.71 to 5.18 μg l?1 and from 0.78 to 20.34 μg l?1, respectively. The mean values of chlorophyll and primary productivity were 1.94 mg m?3 and 938.1 mg C m?2 day?1 in the coastal waters and 4.3 mg m?3 and 636.5 mg C m?1 day?1 in the estuarine waters, respectively.POCchl ratios were low in June and October even when POC values were quite high. The POC in surface waters was linearly correlated with the chlorophyll content. Also PP increased when chlorophyll and primary productivity remained high. The results suggest that the phytoplankton was sharply increasing and contributed to POC and PP content. The percentage of detritus calculated from the intercept values of chlorophyll on POC varied from 46 to 76% depending on season. Results indicate that the major portion of POC and PP during postmonsoon (October–January) is derived from phytoplankton production while the allochthonous matter predominate during monsoon (June–September).  相似文献   

5.
The crab Carcinus maenas (L.) and the barnacle Elminius modestus Darwin were exposed to a range of dissolved concentrations of Zn, Cu and Cd for 21 days in artificial seawater. Accumulation of Zn and Cu by crabs has been interpreted in terms of the presence of a regulation mechanism to maintain constant body concentrations (83·2 ± 19·4 μg Zn g?1 dry wt.; 39·8 ± 9·8 μg Cu g?1 dry wt.) under varying external dissolved metal levels, until a threshold dissolved metal concentration (c. 400 μg Zn l?1; c. 170 μg Cu l?1) beyond which net accumulation of metal begins. Cadium appears to be accumulated by C. maenas at all exposures with no evidence for regulation of body cadmium concentrations. Exposure of E. modestus to Zn, Cu or Cd caused net accumulation of the respective metal in the bodies of the barnacles, with no evidence for regulation of body metal concentrations.  相似文献   

6.
Phytoplankton chlorophyll concentrations in the Delaware estuary range over two orders of magnitude and display several maxima over the seasonal cycle. These maxima were found to be regulated both spatially and temporally by light availability. Both the spring chlorophyll maximum, which reaches 50–60 μg chlorophyll l?1 during a Skeletonema costatum dominated bloom, and transient fall blooms (15–20 μg l?1) are focused in mid-estuary. These blooms are regulated spatially by settling out of suspended sediment below the turbidity maximum and both spatially and temporally by physical factors (e.g. river flow) that cause vertical stratification in mid-estuary. In freshwater regions, chlorophyll concentrations display seasonal periodicity correlated with solar irradiance; summer chlorophyll concentrations average 30 μg l?1. These freshwater and mid-estuarine biomass maxima may be correctly predicted using a steady-state light-limitation model. In contrast, summer chlorophyll concentrations in the lower estuary remain below 10 μg l?1 and are not correctly modeled, despite minimum turbidity, and non-nutrient limiting conditions. These chlorophyll concentrations appear to be regulated by a combination of light availability and grazing.Although extremely high anthropogenic nutrient inputs in the freshwater region of the Delaware River provide non-limiting nutrient concentrations throughout the estuary, regulation of phytoplankton growth by light-limitation restricts chlorophyll concentrations below the nuisance levels found in many eutrophic systems.  相似文献   

7.
Particulate nitrogen (PN) and chlorophyll a (Chla) were measured in the northern reach of San Francisco Bay throughout 1980. The PN values were calculated as the differences between unfiltered and filtered (0·4 μm) samples analyzed using the UV-catalyzed peroxide digestion method. The Chla values were measured spectrophotometrically, with corrections made for phaeopigments. The plot of all PNChla data was found to be non-linear, and the concentration of suspended particulate matter (SPM) was found to be the best selector for linear subsets of the data. The best-fit slopes of PNChla plots, as determined by linear regression (model II), were interpreted to be the N: Chla ratios of phytoplankton. The Y-intercepts of the regression lines were considered to represent easily-oxidizable detrital nitrogen (EDN). In clear water ( < 10 mg l?1 SPM), the N: Chla ratio was 1·07 μg-at N per μg Chla. It decreased to 0·60 in the 10–18 mg l?1 range and averaged 0·31 in the remaining four ranges (18–35, 35–65, 65–155, and 155–470 mg l?1). The EDN values were less than 1 μg-at N l?1 in the clear water and increased monotonically to almost 12 μg-at N l?1 in the highest SPM range. The N: Chla ratios for the four highest SPM ranges agree well with data for phytoplankton in light-limited cultures. In these ranges, phytoplankton-N averaged only 20% of the PN, while EDN averaged 39% and refractory-N 41%.  相似文献   

8.
A relatively precise and rapid method for the analysis of total dissolved monosaccharides at the concentrations that occur in seawater is described which uses 1-ml quantities for each analytical and control replicate. The alditols remain unchanged while the pentoses and hexoses are reduced to the alditol form by borohydride. The total alditols are then oxidized with periodate to form two moles of formaldehyde per mole of monosaccharide and the formaldehyde is determined spectrophotometrically with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH). Unlike other spectrophotometric methods, similar curves are obtained for equimolar concentrations of different carbohydrates while the differences on a weight basis are only due to the small difference in molecular weight between pentoses and hexoses. Winter—Spring samples from surface waters of lower Narragansett Bay ranged from 122 to 226 μg l?1 with a mean of 159 μg l?1.  相似文献   

9.
A method for the determination of barium in sea water was investigated using inductively coupled plasma emission spectrometry, and sea water samples from the Japan Sea and the Pacific Ocean were directly analyzed by this method. Artificial sea water was used to prepare matrix matched standard solutions to overcome the problem of physical interference. The detection limit (signal/noise ratio=2) for barium in deionized and distilled water was 0.08µg l?1 and in sea water, 0.12µg l?1. The reproducibilities in the purified water and in the sea water at the 10µg l?1 level were 0.7% a#FFFFFFnd 0.5%, respectively. The barium concentration in both the Japan Sea and the Pacific Ocean increased with depth and ranged between 5.5–10.0µg l?1 and 4.1–18.4µg l?1, respectively.  相似文献   

10.
Abstract

The influence of temperature on the acute toxicity of a technical formulation (86%) and pure formulation (99%) of pentachlorophenol (PCP) to less than 24‐h‐old Simocephalus vetulus neonates was determined with 48‐h static toxicity tests. The technical grade PCP was significantly more toxic to S. vetulus than the pure PCP (P < 0.05). Sensitivity of S. vetulus to technical PCP also significantly increased with temperature (P < 0.05), but a significant temperature effect was not found with the pure PCP. The mean 48‐h LC50 values for neonates exposed to technical PCP were 140 and 199 μg l?1 at 22°C and 16°C, respectively, and for those exposed to pure PCP were 262 and 304 μg l?1, respectively.  相似文献   

11.
Abstract

Thirty sites were sampled in three New Zealand rivers (Waikato, Maitai, and Wakapuaka) during late summer 1977. Samples were collected from just below the surface at mid river or in the tailraces below hydro‐electric dams.

Parameters measured included bacterial numbers (direct counts), heterotrophic potential (Vmax ), adenosine triphosphate (ATP), chlorophyll a (Chi a), and concentrations of nitrogen and phosphorus compounds.

Bacterial populations per millilitre fluctuated threefold (6.4–19.4 × 105) along the Waikato River and were lower and more consistent in the two South Island rivers (1.46–2.55 × 105). In contrast, Vmax varied 5000‐fold in the Waikato River, from a characteristically oligotrophic value of 0.0035 μg. l?1·h?1 (Lake Taupo outlet) to a eutrophic value of 18.4 μg. l?1·h?1 at the Mihi bridge. Vmax for the two South Island rivers ranged from 0.0091 to 0.189 μg. l?1 · h?1.

ATP, Chi a, Kjeldahl nitrogen, nitrate nitrogen, and total phosphorus concentrations for the 20 sites on the Waikato River varied in a similar way to the Vmax and bacterial data. There were large peaks at the Mihi bridge, lower values for the dam tailraces and significant increases for the sites below Hamilton. Concentrations for these parameters were lower and more consistent along the lengths of the two South Island rivers.

Most parameters were significantly correlated with each other for the Waikato River samples. The strongest correlations were between Vmax and bacterial numbers and between Vmax and nitrate nitrogen. In the Maitai and Wakapuaka River series these correlations were also significant, but the only other significant correlations recorded there were between ATP and nitrate nitrogen, and between ATP and bacterial numbers.  相似文献   

12.
Volatile fatty acid (VFA) apparent turnover rates in organic-rich marine sediments were determined by measuring whole sediment VFA concentration and the corresponding first-order reaction rate constants. In order to measure VFA concentrations, bulk wet sediment samples were basified, freeze-dried, extracted with methanol, derivatized to form methyl esters of the VFAs, and analyzed by packed-column gas chromatography using hexanoic acid as an internal standard. The detection limits for acetate, propionate, iso-butyrate and butyrate were 1.0, 0.4, 0.2 and 0.2 μmol l?1s, respectively, for 600 ml samples. Rate constants for acetate and propionate were determined by anaerobically incubating samples at in-situ temperatures with tracer levels of 14C-labelled VFAs. Metabolized label was recovered as CO2, CH4, cellular material, water-soluble material, and VFA (ether-soluble) fractions. Apparent turnover rates measured during summer and winter in anoxic Cape Lookout Bight, North Carolina (U.S.A.) sediments were in the range of 19–330 μmol l?1s h?1 for acetate and 0.7–7.0 μmol l?1s h?1 for propionate.  相似文献   

13.
Progress in the introduction of coulometry for the analysis of total carbon dioxide (TCO2) in marine waters is described. An extractor—stripper removes CO2 that is measured coulometrically by the quantity of electricity (coulombs) used to electrogenerate OH? ions for the titration of the acid formed by the reaction of CO2 and ethanolamine. The equivalence point is detected photometrically with thymolphthalein as the indicator, and Faraday's Law relates coulombs to equivalents of titrant generated and CO2 determined so that there are no standard curves needed or titrants to standardize or store. Accuracy was determined by adding gelatin capsules containing 100–1500 μg C of pure CaCO3 into the stripper, and accuracies of better than ± 1 μg C were achieved. The best precision for natural seawater (± 1 standard error) of ± 0.5 μmol l?1 was found for 17 samples of Bermuda coastal waters having a mean TCO2 of 2007.2 μmol l?1 (0.05% CV). Sources of error and precautions are discussed. This method, which has been used successfully at sea, can be used to study a variety of marine phenomena involving TCO2.  相似文献   

14.
Anaerobic salt marsh sediments were amended with a variety of organic pollutants and the effects on methanogenesis, sulfate reduction and carbon dioxide evolution were examined. Addition of 1000 μg g?1 (dry weight sediment) Arochlor 1221, lindane, endrin, benzene and phenanthrene resulted in no significant effects on the activities studied. Methanogenesis was inhibited by 1000 μg g?1toxaphene, PCP, chlordane, naphthalene, DDT, Kepone and heptachlor and by 100 μg g?1 PCP and toxaphene. At 1000 μg g?1 naphthalene and toxaphene and 100 μg g?1 PCP, a period of initial inhibition of methanogenesis was followed by stimulation relative to controls. Arochlor 1254 (1000 μg g?1) and Temik (500 and 10 μg g?1) stimulated methanogenesis from the outset. Temik at 500 μg g?1 gave the greatest stimulation of methanogenesis (900% of controls) of any of the compounds studied. Sulfate reduction was inhibited by 1000 μg g?1 PCP, toxaphene, naphthalene and chlordane and by 500 μg g?1 atrazine and 100 μg g?1 heptachlor. Sustained inhibition of sulfate reduction by naphthalene, toxaphene and PCP may have contributed to the stimulation of methanogenesis. Carbon dioxide evolution was not significantly affected by most of the compounds studied except for 100 μg g?1 PCP and 1000 μg g?1 aphthalene, each of which gave significant inhibition in only one of three experiments.Concentrations of individual organic pollutants required to cause observable effects were high. It is concluded that, except for highly polluted sediments, methanogenesis, sulfate reduction and CO2 evolution would not be affected by the compounds studied here at concentrations typically found in the environment.  相似文献   

15.
The effects of cresols on the photosynthesis and growth of natural marine phytoplankton assemblages from the Scripps Pier at La Jolla, California, USA were measured. In two experiments, in which dinoflagellates were the dominant algae, photosynthesis (measured by 14C uptake) was inhibited at concentrations > 100 μg litre?1 and the concentration giving half-maximal photosynthesis was 10,000 μg litre?1. In a third experiment, in which diatoms were dominant, photosynthesis was not inhibited until levels > 10,000 μg litre?1 were reached and the half-maximal concentration was 20,000 μg litre?1. These experiments suggested that dinoflagellates were more sensitive to cresols than diatoms. However, the reverse was true in an experiment using cultures. In a long-term growth experiment (natural assemblages from the Scripps Pier), only a level of 88,000 μg litre?1 completely inhibited growth. There was a two-day lag in growth at 8500 μg litre?1, but the algae recovered and grew as well as the controls which contained no cresols. This experiment was started with a mixed diatom-dinoflagellate-microflagellate population but only the diatoms grew. High concentrations of cresols (> 900 μg litre?1) resulted in a decrease in the diatom Skeletonema costatum, compared with the controls. Otherwise there was no selection for resistant species. In these experiments, cresols were measured by extraction with methylene chloride from seawater followed by measurement by UV absorption. In all experiments cresol levels decreased with time. This was attributed to uptake by phytoplankton and bacterial degradation.  相似文献   

16.
Nutrient and chlorophyll a concentrations and distributions in two adjoining regions of the South Atlantic Bight (SAB), Onslow Bay and nearshore Long Bay, were investigated over a 3-year period. Onslow Bay represents the northernmost region of the SAB, and receives very limited riverine influx. In contrast, Long Bay, just to the south, receives discharge from the Cape Fear River, draining the largest watershed within the State of North Carolina, USA. Northern Long Bay is a continental shelf ecosystem that has a nearshore area dominated by nutrient, turbidity and water-color loading from inputs from the river's plume. Average planktonic chlorophyll a concentrations ranged from 4.2 μg l−1 near the estuary mouth, to 3.1 μg l−1 7 km offshore in the plume's influence, to 1.9 μg l−1 at a non-plume station 7 km offshore to the northeast. Average areal planktonic chlorophyll a was approximately 3X that of benthic chlorophyll a at plume-influenced stations in Long Bay. In contrast, planktonic chlorophyll a concentrations in Onslow Bay were normally <0.50 μg l−1 at a nearshore (8 km) site, and <0.15 μg l−1 at sites located 45 and 100 km offshore. However, high water clarity (KPAR 0.10–0.25 m−1) provides a favorable environment for benthic microalgae, which were abundant both nearshore (average 58.3 mg m−2) and to at least 45 km offshore in Onslow Bay (average 70.0 mg m−2) versus average concentrations of 10–12 mg m−2 for river-influenced areas of Long Bay. This provides evidence that much of the inner shelf food web in Onslow Bay is based on benthic microalgal production, in contrast to a plankton-based food web in northern Long Bay and more southerly areas of the SAB.  相似文献   

17.
Pacific oyster, Crassostrea gigas Thunberg, embryos were exposed to copper and silver salts, alone and in combination, through the completion of embryogenesis and metal effects upon normal embryonic development were monitored. Copper concentrations throughout the test period ranged from 0·0 to 12·0 μg litre?1 while silver concentrations were between 0·0 and 18·0 μg litre?1.Administered individually, silver and copper yielded decreasing per cent normal embryonic development with increasing metal concentration. However, both metals exhibited ‘plateaus’ wherein per cent normal embryonic development did not decrease with increasing metal concentration until a particular concentration was attained. A rapid decrease in per cent normal embryonic development occurred with increasing metal concentrations greater than 6·0 μg litre?1 copper and 11·0 μg litre?1 silver.The dose-response patterns of copper and silver appear to be similar with copper exhibiting somewhat greater toxicity. At a copper concentration of 10 μg litre?1 only 50% of the embryos could be considered normally developed, whereas silver concentrations between 16·0 and 18·0 μg litre?1 yielded 50% normal embryonic development.Response surface methodology indicated that copper and silver interacted additively, with embryonic development affected to a much greater degree by copper than by silver. Optimum development of Crassostrea gigas embryos was greatly influenced by copper concentrations and occurred at all silver concentrations wherein the corresponding copper concentrations were 6·0 μg litre?1 or less. Above 6·0 μg litre?1 copper, per cent normal embryonic development was significantly reduced, even at the lowest tested silver concentrations.Abnormal embryos exhibited retarded shell growth, reduced size and extremely erratic swimming behaviour. These sublethal effects probably have a biochemical origin and would limit the organism's capacity to feed properly, escape predation and develop normally, thereby reducing the chances of successful metamorphosis and recruitment into the adult population.  相似文献   

18.
Abstract

Oxygen consumption, grazing rate, and byssus production of the greenshell mussel Perna canaliculus were measured following exposure to bloom concentrations (106 cells l?1 ) of a toxic clone of the dinoflagellate AIexandrium tamarense (GT429), using anon‐toxic clone of the same species (PLY173) as control. Following feeding for 1 h on GT429, mussels (dry weight 54–127 mg) showed significantly increased oxygen uptake at 15°C (0.08 μl O2 (mg dry tissue weight)?1 ) compared with control mussels. However, following 24 h recovery, oxygen uptake was similar to initial values in all experimental conditions. The grazing rate of P. canaliculus fed upon toxic clone GT429 was 4.58 ± 0.76 cells mg?1 h?1 compared with 1.99 ± 0.47 cells mg?1 h?1 for PLY173. On exposure to A. tamarense, mussels maintained normal opening behaviour and there was no change in the byssus production over 24 h. It was concluded that P. canaliculus showed no dramatic physiological effects following short‐term feeding on the toxic strain of A. tamarense. During 2 weeks’ exposure to twice daily bloom concentrations of GT429, there was no mussel mortality. Toxicity in the tissues was 1295 μg STX equivalent per 100 g tissue, levels at which these mussels would be unsuitable for human or animal consumption.  相似文献   

19.
Cd, Cu, Fe, Ni, Pb and Zn were determined in 123 samples from the Baltic Sea proper. The trace metals were extracted directly on board the vessel, using a dithiocarbamate-Freon procedure. Final analyses of the extracts are performed onshore by atomic absorption spectrometry.Similar trace-metal concentrations are found in different areas of the Baltic proper. Most values fall in the following ranges: Cd, 30–60 ng 1?1; Cu, 0.6–1.0 μg 1?1; Fe, 0.3–0.9 μg 1?1; Ni, 0.6–0.9 μg 1?1; Pb, 0.05–0.2 μg 1?1; and Zn, 1.5–3.5 μg 1?1. The metal-concentrations are generally independent of depth. However, copper exhibits a small but significent decrease in concentration below 80 m.Filtration did not affect trace-metal concentrations, with the exception of iron in waters from lower layers. Similarly, storage under acid conditions was shown to affect only the concentration of iron. An electro-chemical technique was also used to determine Cu in some samples.  相似文献   

20.
A spectrophotometric procedure for the determination of aldehydes was optimized for use in seawater, it involves the sequential reaction of aldehydes with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and FeCl3 to produce a colored compound which is soluble in 50% acetone. The standard curve obeyed Beer's law to 90 μM formaldehyde in 0.7 m NaCl. The molar absorptivity of 21 800 absorbance units μM?1 cm?1 at 635 nm was not affected by changes in salinity. The limit of detection was 180 nM HCHO l?1 when a pathlength of 1 cm was used, and 72 nM HCHO l?1 when a pathlength of 5 cm was used.The MBTH procedure was used to compare formaldehyde production in cultures of the marine methanotroph, Methylomonas pelagica, growing on methane or methanol. The average rate of formaldehyde production, normalized to cell number, was almost 20 times greater in cultures grown on methanol than in cultures grown on methane.A depth profile of aldehyde concentrations from a station in the Peru upwelling region (10°S, 79°W) showed one peak in the oxygen gradient in the photic zone (80 m), two in the oxygen minimum (200 and 300 m), and one in the oxygen gradient below the minimum (800m). Aldehyde concentrations ranged from 0.6 to 8.8 μM formaldehyde equivalents l?1. Except for the maxima where the aldehydes account for 13–15% of expected DOC concentrations, the background level of aldehydes was approximately 1% of DOC.  相似文献   

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