共查询到20条相似文献，搜索用时 31 毫秒
1.
A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo Ⅰ, Hae Ⅲ, and RSA Ⅰ) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P arcuatum (ME 131), which had the same pattern to P. micans (ME 160 and 04).The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electrophoresis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum(MEI32) could not be differentiated from P. micans (MEI60 and 04), it was probably mis-identified, since they are quite different morphologically. P. redfieldii (MEI38) could also not be distinguished form P. triestinium(MEI32), it should be regarded as a synonym ofP. triestinium. Unexpectedly, a restriction site was found in P.micans, compared with previous sequence data. 相似文献
2.
AmpLifications with 300 10-base primers under predetermined optimal reaction yielded 2762 reproducible amplified fragments ranging from 200 to 3000 bp. Genetic distances among five species of Takifugu and Lagocephalus spadiceus as outgreup were calculated based on the presence/absence of the amplified fragments. Topological phylogenie trees of the 5 species of Takgfugu and the outgroup were gen-erated by Parsimony and Neighbor-Joining analysis based on RAPD data set. The genetic distance between T. rubripes and T. pseudommus was almost the same as that between individuals within each species, but was much smaller than that among T. rubripes, T. pseudommus and the other species. The molecu-lar data from RAPD analysis convincingly showed that T. rubripes and T. pseudommus should be the same species. 相似文献
3.
Laboratory culture experiments showed that <100μ mol/L nitrate, amonium or mixture of amino acids promote the growth of the
red tide organismProrocentrum micans Ehrenb, but that >100μmol/L of ammonium, or mixture of glycine and glutamate was harmful to growth, and that orthophosphate
wasP. micans’ main phosphorous source in the ocean. Presence of 80μ mol/L EDTA, 0.5 to 1 μmol/L Fe3+, 1.0 to 20.0 μ mol/L Mn2+ 0.1 to 0.4 μmol/L Co2+ in the culture medium could improve the growth ofP. micans. Vitamin B1 promoted growth, but vitamin B12 and biotin did not. The estimated minimum cell quotas (q
o) for nitrogen and phosphorus being 0.74 pmole/cell and 0.045 pmole/cell show that phosphorus (more than nitrogen) limits
the growth ofP. micans in the study area.
This project was supported by the Natural Science Foundation of Zhejiang Province. 相似文献
4.
He Yingjun Zou Yuping Wang Xiaodong Zheng Zhiguo Zhang Daming Duan Delin 《中国海洋湖沼学报》2003,21(2):141-148
Eighteen gametophytes includingL. japonica, L. ochotensis andL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers
selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance
among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic
(UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively.
It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria, but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship
if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship
among the species ofLaminaria.
Contribution No. 4389 from the Institute of Oceanology, Chinese Academy of Sciences.
This research was supported by the State's High-tech Project of China, Marine 863 project (No. 819-03-02) and (2001AA621090). 相似文献
5.
Sonia MUNIR Zaib-un-nisa BURHAN Tahira NAZ P.J.A.SIDDIQUI Steve L.MORTON 《中国海洋湖沼学报》2013,31(2):267-281
Morphotaxonomy and seasonal abundance of dinoflagellates of the genera Prorocentrum and Mesoporos(Prorocentrales) were studied from nutrient-rich waters,Karachi Harbor and the mouth of the Manora Channel,Pakistan during May 2002-July 2003.Using both light and scanning electron microscopy,13 species of Prorocentrales were identified according to cell shape,size,ornamentation of thecal plates,and architecture of apical platelets,apical pore area,marginal pores,and intercalary bands.P.sigmoides,P.arcuatum,P.scutellum,P.donghaiense,P.balticum,P.minimum,P.emarginatum,P.lima,P.faustiae,and Mesoporos perforatus constitute new records for sindh coast of Pakistan.The most abundant species were P.minimum/P.balticum(4.5×103 cells/L),P.micans(1.1×103 cells/L),P.gracile/P.sigmoides(2.5×102 cells/L) and P.donghaiense(6.6×103 cells/L) at temperatures of 29-31℃ and salinities of 35-40.Maximum abundance was observed in winter and lower abundance in summer.There was no significant change in the distribution of species between stations except for the benthic species which occurred close to Karachi Harbor waters.Significant positive correlations were observed between Prorocentrum spp.and temperature(R 2 =0.27) and negative correlations with salinity(R 2 =-0.32) except for P.minimum and P.emarginatum which has negative correlation with temperature(R 2 =-0.24) and positive with salinity(R 2 =0.08,0.19).The finding of potential okadaic-acid producing species of benthic Prorocentrum call for monitoring for possible human health problems in this region. 相似文献
6.
The morphology and toxicity ofPseudo-nitzschia strain PP0201-01, isolated near the estuary of Changjiang (Yangtze) River in Year 2002 and tentatively identified asPseudo-nitzschia pungens, were studied. The strain was identified morphologically asP. pungens using scanning electronic microscope (SEM). The analytical results of LC-PDA (liquid chromatography with photodiode array
detector), LC-MS (liquid chromatography with mass detector), and the toxicity data from mouse bioassay, showed that no domoic
acid was produced by the strain. However, the mechanism of domoic acid production byPseudo-nitzschia species is complicated. More works are needed to assess the toxic species and to evaluate the potential risk of amnesic shellfish
poisoning (ASP) in China.
Supported by the projects of Ministry of Science and Technology (No. 2001CB409704 and No. 2001BA804A20), and the innovation
project of the Institute of Oceanology, CAS (No. 200223107). 相似文献
7.
Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships
in 4 species ofPorphyra. The samples were collected from the coast of Canada, Vietnam, Zhoushan, Fujian and Qingdao in China. Amplifications with
20 primers were carried out under predetermined optimal reaction conditions (samples were first heated at 94°C for 5 min.
and followed by 45 cycles of 1 min at 94°C, 1 min at 36°C, and 2 min at 72°C, then held at 72°C for 10 min). The amplified
products were scored as present (1) or absent (0) for each DNA sample and an index of genetic similarity (F) was calculated
by using Nei & Li’s matching coefficient method (1979). The value of (1−F) was used to quantify the genetic distances between
species and construct a phylogenetic tree. The relationship indicated by the UPGMA and NJ cluster analysis on the values of
the genetic distance is in good overall agreement with classical taxonomy. The obvious differences between natural and cultivated
population ofP. haitanensi suggest that variation or hybridization with other species occurred during the culture.
Contribution No. 100 from the Experimental Marine Biology Laboratory, IOCAS.
Contribution No. 3289 from the Institute of Oceanology, Chinese Academy of Sciences.
The research supported by the Foundation of the Chinese Academy of Sciences. 相似文献
8.
ZHANG Zhengbin* LI Peifeng and LIU Chunying Institute of Marine Chemistry Ocean University of China Qingdao P.R.China 《中国海洋大学学报(英文版)》2006,5(3):239-242
1Introduction NOisadiffusible,gaseousfreeradicalthatplaysimportantrolesinanimalsandplants.Itfunctionsin diseaseresistance,abioticstress,celldeath,respira tion,senescence,rootdevelopments,germinationandhormoneresponses.Itisanunusualsignalinthatitis areactive,lipophilicandvolatilefreeradicalthatcanbecytotoxic.ThewidespreadbiologicalsignificanceofnitricoxidewasrecognizedbySciencein1992whichnamedthefreeradicalNO‘Moleculeoftheyear’,andin1998theNobelPrizeinPhysiologyandMedicinewasawardedforwor… 相似文献
9.
Observation of female gamete (Carpogonia) germination of Porphyra yezoensis and P. oligospermatangia
Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades
and conchocelis in the same culture ofPorphyra. Study on samples of wild and cultivatedPorphyra yezoensis andP. oligospermatangia were conducted to clarify the origination of the young blades in the culture. It is confirmed that single cells on the blade
of both species, which normally intermixed with zygotospores, germinated into young blades. TEM and SEM observation has shown
that the single cells ofPorphyra yezoensis had typical features of female gamete (carpogonia) but archeospore. Therefore, the female gametes are responsible in developing
leafy thalli.
This project was sponsored by the NSFC (No. C-0205-05-39770593) 相似文献
10.
Applying randomly amplified polymorphic DNA (RAPD), the genetic variation ofCabomba caroliniana Gray (cabomba or fanwort), a new alien plant in China, was analyzed in this paper. Total 143 bands, including 47 polymorphic
bands, were amplified from 23 primers in 20 samples. The sampling distance was large, but its genetic diversity was low. The
main results were that: (1) Cabomba, which grew and dispersed mainly in fragment, was an abundant and dominant species in
freshwater, and its main dispersal mechanism was vegetative reproduction (2) Cabomba was originally introduced into China
as an aquarium submerged plant. Somehow, those discarded cabomba became invasive species in the areas of Hangzhou, Shanghai,
and Meicheng, and other places. (3) Although the level of genetic diversity in cabomba was low, their rapid dispersion and
propagation could seriously harm to local aquatic community. Therefore, specific measure should be used to control cabomba
from uncontrolled spreading and damage to local vegetation communities.
Supported by National Natural Science Foundation of China (No. 39970145) and Major State Basic Research Development Program
of China (No. G2000046803). 相似文献
11.
British Antarctic Survey (BAS) data collected near the Antarctic Peninsula area were used in this 1988/1989 study on the length
frequency of three Antarctic fish species (Pleuragramma antarcticum, Electrona antarctica and Protomyctophum bolini) showing that the standard length (SL) ofP. Antarcticum ranged from 26.0 mm to 161.0 mm, that the 26.0 mm to 54.0 mm SL juveniles could be considered to be the age group 1 fish,
and that the SL ofE. antarctica ranged from 39.4 mm to 89.7 mm for the males and 48.0 mm to 118.0 mm for the females, which may contain at least 6 age groups.
This is more than previously thought.P. Bolini had 29.2 mm to 60.2 mm SL and complicated age composition. 相似文献
12.
Nie Pin 《中国海洋湖沼学报》1997,15(1):61-64
A new species ofProteocephalus from the fishSinocyclocheilus grahami tingi, a subspecies endemic to Fuxian Lake in Southwest China,is described. This is the first report of cestode from this host. The new species differs fromP. exiguus, P. Longicollis, P. parasiluri andP. torulosus, the only other species ofProteocephalus currently reported from China, by lacking an apical sucker, fromP. exiguus by having 45–108 rather than 24–54 testes per proglottis, and a maximum of 15 rather than 28 uterine diverticula; fromP.longicollis by having an average of 45–108 rather than 75–115 testes per proglottis and testes being in a single layer instead of 2 layers;
and fromP. torulosus by having 45–108 instead of 150 testes per proglottis and testes being in 3–4 layers; and fromP. parasiluri by having 45–108 rather than 180 testes per proglottis. It resemblesP. percae, P. pollanicola, andP. thymalli in number of testes and uterine diverticula, but differs by lacking an apical sucker.Proteocephalus thymalli, like the new species, has a vagina that opens dorsally to the cirrus sac, butP. thymalli slao has postovarian testes, which the new species lacks. The possible significance of parasitological examinations of the
other two subspecies ofS. grahami in two lakes close to Fuxian Lake is discussed in relation to host speciation and coevolution of the host-parasite system. 相似文献
13.
14.
Batrachospermum arcuatum Kylin is typically dioecious,although some monoecious specimens have been collected from four locations in North China.In this study,B.arcuatum populations,including monoecious and dioecious thalli,were collected from seven stream segments.The nuclear DNA internal transcribed spacer(ITS)region was sequenced for the seven populations,and a phylogenetic tree was constructed using Bayesian inference to assess intraspecifi c relationships.A haplotype network was also created.The ITS region in B.arcuatum from North China comprised 822–853 bp,with 10 haplotypes detected from the seven locations.The results of this study support the inclusion of monoecious individuals in the species B.arcuatum. 相似文献
15.
Isabella A. Abbott 《中国海洋湖沼学报》1995,13(4):343-347
A new species ofLiagora, L. perennis sp. nov. from Hawai’i, is described. Unlike species of this genus in China, it is carpotetrasporangial, and at the place
of expected carposporangia bears cruciately divided tetrasporangia, a detail which differentiates it from the usual species
ofLiagora without tetraspores that have been reported in China. Notes are provided on otherLiagora species that have an impact on the taxonomy of species found in China.
Dedicated to the memory of Zhang Junfu(C. F. Chang) whose studies on marine algae I respect highly, and whose good-humored
friendship I treasure. 相似文献
16.
Results of the experiment of northward transplantation ofPorphyra haitanensis showed that intercrop ofP. haitanensis with Bay Scallop is feasible because the growth duration of both of them is about the same in August–December.
Full use of cultivating area, lowering its cost and increasing its product per unit area as well as improving the ecological
environment are realized during intercrop.
Contribution No. 2152 from the Institute of Oceanology, Academia Sinica. 相似文献
17.
Results of neutron activation analysis (NAA) ofSargassum thumbergii samples collected from the Qingdao Taipingjiao coast on March 20, 1996 showed that (1)Sargassum thunbergii can to some extent accumulate potassium and calcium (the accumulation coefficient was 10); (2) it can accumulate almost all
trace elements, especially iron, managanese and zinc; (3) it can accumulate strontium, aluminium thorium, and rare earth elements.
Contribution No.3308 from the Institute of Oceanology, Chinese Academy of Sciences.
The study supported by the National Ninth-Five-Year Key Project “96-916-04-01”. 相似文献
18.
19.
Inorganic carbon utilization in the non-calcifying marine microalgae,Nannochloropsis oculata, Phaeodactylum tricornutum andPorphyridium purpureum was compared with high- and low-calcifying strains ofEmiliania huxleyi grown in artificial seawater medium aerated with either air (0.03% V/V CO2) or CO2-free air. For high-calcifying strain ofE. oculata andP. tricornutem, similar growth patterns were observed in air-and CO2-free air-grown cultures.P. purpureum showed a less final cell density in CO2-free air than in air-grown culture. However, low-calcifying strain ofE. huxleyi was able to grow only in air-grown culture, but not in CO2-free air-grown culture.
Measurements of alkalinity, pH, concentration of dissolved inorganic carbon (DIC) and free CO2 showed different patterns of DIC utilization. WithN. oculata, P. tricornutum andP. purpureum the pattern of DIC utilization was characterized by an increase of pH and a decrease of DIC but a constant alkalinity in
the cultures aerated with air or CO2-free air, suggesting that bicarbonate utilization was concomitant with an efflux of OH−. Both alkalinity and pH were maintained rather constant in air-grown culture of low-calcifying strain ofE. huxleyi, suggesting that diffusive entry of CO2 could meet the requirement of DIC for its photosynthesis and growth. High-calcifying strain ofE. huxleyi, however, showed a pattern of decrease of alkalinity and DIC but an almost constant pH, indicating that bicarbonate was the
major form of inorganic carbon utilised by this organism and bicarbonate uptake is unlikely to be accompanied by an efflux
of OH−.
The final pH values reached byN. oculata, P. tricornutum andP. purpureum in a closed system were 10.75, 10.60 and 9.85 respectively, showing that bicarbonate utilisation is concomitant with an efflux
of OH−. While the final pH of 8.4 in high-calcifyingE. huxleyi suggests that bicarbonate utilization was not accompanied by an efflux of OH−.
Contribution No. 3557 from the Institue of Oceanology, Chinese Academy of Sciences.
This work was supported by NERC grant GRE3/7853 U. K. and partly supported by Bio-Engineering Center, SSTC 96-C01-05-01. 相似文献
20.
Nephroselmis gaoae sp. nov. is described on the basis of light and electron microscope observations of cultured material originally collected
and isolated from seawater of Jiaozhou Bay, Qingdao, China. The periplasts on the cell body and flagella are covered by five
types of scales, two types on the flagella and three on the body. Among these, the morphology and the number of spines of
large stellate body scales differ remarkably from those of previously described species ofNephroselmis. Apart from these, the unusual fine structure of the eyespot (stigma) is very characteristic. As in the other species ofNephroselmis, the eyespot lies immediately under the two-membraned chloroplast envelope; unlike the others, however, it is not composed
of a number of osmiophilic globules, but consists of about 14 curved rod-shaped osmiophilic bodies arranged loosely and randomly.
This feature distinguishes the present new species not only from the other species ofNephroselmis but also from the other motile algal species, the eyespots structure of which had been previously described.
Contribution No. 2316 from the Institute of Oceanology, Chinese Academy of Sciences.
This paper was presented at the XIVth International Seaweed Symposium held in Brittany, France, 1992. 相似文献