共查询到18条相似文献，搜索用时 250 毫秒
1.
米曲霉中性蛋白酶特性的研究 总被引：2，自引：0，他引：2
研究了米曲霉中性蛋白酶的特性。结果表明，米曲霉中性蛋白酶的最佳提取方法为加入pH7．2的缓冲液研匀，置恒温（30℃）培养箱中提取30min．每10min搅动1次，最后用4层纱布过滤。酶反应最适温度为55℃，最适pH值为7．2。NaCl和EDTA对其有一定的抑制作用。酶的热稳定性好．在45℃的温度下处理24h后，酶活力仍有45．5％。米曲霉中性蛋白酶与培养物一起保存时，稳定性也较好，在4℃保存一个月后，酶活力几乎无变化。 相似文献
2.
米曲霉产中性蛋白酶的适宜条件 总被引：3，自引：0，他引：3
研究了米曲霉产蛋白酶的分布,优化了米曲霉产中性蛋白酶的适宜培养条件以及培养基的最优组成。研究发现米曲霉产中性蛋白酶的能力为最强。米曲霉产中性蛋白酶的适宜培养条件为:m(麸皮)∶m(豆粕粉)=4∶1,水的质量分数为60%,培养基中各无机盐质量分数为:KNO30.2%,MgSO40.05%,Na2HPO40.13%,pH值为6.0,接种量为每10 g培养基接种1.0×108个孢子,最佳培养温度为30℃,最佳培养时间为48 h。在此培养条件下,最高酶活力达3 999.2 U.g-1。 相似文献
3.
几种因子对鞍带石斑鱼精子活力的影响 总被引：3，自引：0，他引：3
研究不同梯度的盐度、pH、温度及低温保存时间对鞍带石斑鱼精子活力的影响。结果表明:鞍带石斑鱼精子活力的适宜盐度范围为27.5～35.0,最适盐度为32.5,此时精子的快速运动时间和寿命分别为13.2min和14.8min;最适pH为8.5,精子的快速运动时间及寿命最长,分别达12.7min和17.3min;温度范围为27～31℃,以29℃时快速运动时间最长,达16.6min;在0～4℃冰箱保存6h后鞍带石斑鱼精子活力未显著降低,其中保存前精子快速运动时间为18.4min,保存6h后为18.2min,保存120h后仍有活力,精子快速运动时间为2.3min,精子激活率为3.3%。以x为保存时间,快速运动时间y和寿命y′符合线性方程y=-0.8334x+19.164(R2=0.9856),y′=-1.1358x+27.8(R2=0.9573)。 相似文献
4.
罗非鱼溶菌酶活力的研究 总被引：5，自引：0，他引：5
《广东海洋大学学报》2002,22(3):3-7
采用试管法（测定熔菌酶活力）研究pH和温度对罗非鱼血清，肝，鳃中溶菌酶活力的影响，以确定其最适pH和最适温度，同时研究了溶菌酶对温度的耐受性及其在体外的抑菌效果。结果表明，罗非鱼血清，肝溶菌酶的最适pH是6．30，最适温度是28℃，鳃中溶菌酶的最适pH是6．10，最适温度是34℃。各种酶在50℃保温40min以上将被灭活，在37℃以下活性是稳定的；体外抑菌试验表明罗非鱼溶菌酶对革兰氏阳性菌具有较好的抑菌效果。 相似文献
5.
罗非鱼溶菌酶活力的研究 总被引：1，自引：0，他引：1
丁燏 《广东海洋大学学报》2002,22(3):3-7
采用试管法 (测定溶菌酶活力 )研究pH和温度对罗非鱼血清、肝、鳃中溶菌酶活力的影响 ,以确定其最适pH和最适温度 ,同时研究了溶菌酶对温度的耐受性及其在体外的抑菌效果。结果表明 ,罗非鱼血清、肝溶菌酶的最适pH是 6 3 0 ,最适温度是 2 8℃ ;鳃中溶菌酶的最适pH是 6 1 0 ,最适温度是 3 4℃。各种酶在 5 0℃保温 40min以上将被灭活 ,在 3 7℃以下活性是稳定的 ;体外抑菌试验表明罗非鱼溶菌酶对革兰氏阳性菌具有较好的抑菌效果。 相似文献
6.
《广东海洋大学学报》2020,(3)
【目的】研究热处理温度和时间对凡纳滨对虾肌肉组织蛋白酶L酶活力的影响,并分析其酶活力变化动力学。【方法】从凡纳滨对虾的肌肉组织提取组织蛋白酶L,研究加热温度(35～60℃)和时间(5～60 min)对其酶活力的影响,采用反应动力学模型拟合酶活力的变化过程。【结果】在35℃组织蛋白酶L的酶活力变化适合用一级动力学模型来描述,而在40～60℃组织蛋白酶L的酶活力变化适合用两阶段动力学模型来描述;凡纳滨对虾肌肉组织蛋白酶L存在敏感型和稳定型两种同工酶,敏感型的Z_(T,L)和E_(a,L)分别为158.7℃和5.5k J/mol,稳定型的Z_(T, s)和E_(a, s)分别为71.9℃和12 kJ/mol。【结论】加热处理可以使组织蛋白酶L发生变性而失活,采用合理的加热温度和速度可以降低组织蛋白酶L引起的凝胶劣化。 相似文献
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8.
为研究生物酶采油解堵剂中产蛋白酶菌株的初、复筛选及培养条件优化,从大庆原油样品中筛选菌种,通过水解酪素的透明圈实验及福林酚测蛋白酶酶活的方法进行菌株的初、复筛选;以蛋白酶酶活为优化指标,采用单因素实验对筛选的产蛋白酶菌株的培养基及培养条件进行优化,优化最适培养基:可溶性淀粉为15g/L,蛋白胨为20g/L,酵母膏为20g/L,NaCl为1.0g/L,CaCl2为0.02g/L,Na2HPO4为0.2g/L,NaH2PO4为0.1g/L;在初始pH为6.0、接种量为5%(体积分数)、温度为31℃、摇床转速为160r/min的条件下,培养72h后,菌株的蛋白酶酶活为551.0U/mL,为复筛选菌株的蛋白酶酶活的22.92倍,即为菌株生长繁殖及代谢的最佳条件,能够获得更高的蛋白酶酶活,有利于后续实验的进行.结果表明:菌株产蛋白酶对原油作用效果为发酵液表面张力从作用前的56.2mN/m降低到作用后的30.5mN/m,表面张力显著降低,还有降解降黏原油等效果,具有一定的研究价值. 相似文献
9.
报道了实验室分离、筛选的 5株有益芽孢杆菌对温度、对虾饲料制粒工艺流程和pH值的耐受性。结果表明 ,5株芽孢杆菌经 80℃水浴 4 0min后全部存活 ,90℃水浴 2 0min有 35 %～ 70 %存活 ,10 0℃水浴 10min仍有 30 %～ 5 0 %存活 ,显示所筛选的 5株芽孢杆菌对高温有较强的耐受力 ;在对虾饲料中添加 5株芽孢杆菌 ,经整个生产工艺流程后芽孢杆菌存活 95 %,烘干后芽孢杆菌存活 93%,说明杆菌能够承受饲料制粒生产中压力、温度和水分的变化 ;当pH值为 3.8、4 .6、5 .2时 ,分别有 1、3、5株菌能繁殖 ,pH值 6 .0～ 8.5时 ,5株菌生长旺盛 ,将 5株菌经pH值 2 .2～ 4 .6处理1h ,再接种在pH值 7.2的培养基可以良好生长 ,说明杆菌在酸性环境中生长性能较差 ,在弱酸性和碱性环境中生长旺盛 ;能耐受酸性环境而存活 ,再给予适宜环境即能良好生长。 5株芽孢杆菌具有作为水产硬颗粒饲料专用益生素菌株的优势。 相似文献
10.
《广东海洋大学学报》2019,(6)
【目的】探究前处理方式对酶解效果的影响,优化牡蛎短肽制备工艺。【方法】在确定高效酶种类和前处理方式的基础上,以氮回收率、短肽得率为指标对酶解时间、料液比、酶解温度、加酶量进行单因素试验,再利用响应面设计建立数学模型,以短肽得率为响应值,进行4因素3水平的响应面分析。【结果与结论】牡蛎经80℃热处理10 min后使用动物蛋白酶的酶解效果最佳。响应面结果显示,最佳酶解工艺为料液比(g/m L)1∶3.9、温度47℃、加酶量3 300 U/g、自然pH(6.5)、酶解3 h,其短肽得率为(58.53±1.20)%,比原酶解工艺提高24.8%。 相似文献
11.
Dewi Seswita Zilda 《中国海洋大学学报(英文版)》2012,11(2):213-218
A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia’s hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability. 相似文献
12.
A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15℃ and 30℃, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35 ℃ and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease. The presence of Ca2+ and Mn2+ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid (AA) residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family. 相似文献
13.
The new polyenzyme method for making gravy from Harengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50℃ , alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, andflavorase concentration of 2.0% , for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3 % of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5% , total and amino nitrogen being respectively1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy from Harengula zunasi offal. In addition, in inorganic elements, the phosphorus content is the highest. 相似文献
14.
A Gram negative bacterium Ar/W/b/75°25＇N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25＇N, and longitude 162°25＇W in Chukchi sea, Arctic. The strain can grow at the temperature range from 7℃ to 30℃, and grow better at 30(℃. It can not grow at 40℃. Keeping certain salinity concentration in medium is necessary for cell growth. It grows well in medium containing salinity concentration from 0. 5 % to 10 % sodium chloride. Glucose, sucrose and soluble starch can be utilized by the strain, among which glucose is the optimal carbon source. Peptone is the optimal organic nitrogen source for cell growth and protease producing, and ammonium nitrate is the optimal inorganic nitrogen source.About 75.7% of total protease of the strain are extracellular enzyme. Optimal temperature for proteolytic activity is at 40℃. Protease of the strain keeps stable below 40℃, and shows high proteolytic activity within the pH range from 7 to 11. 相似文献
15.
Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the bio-technological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5) , producing cellulose at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 351 or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes i 相似文献
16.
The new polyenzyme method for making gravy fromHarengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal
trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for
the polyenzyme method were pH of 7.0, temperature of 50°C, alkaline and neutral protease concentration of 1.5% respectively,
hydrolysis time of 120 min, and flavorase concentration of 2.0%, for 60 min. The new gravy-making technology yields a nutritious
and delicious gravy containing 40.3% of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and
Ser comprising 49.5%, total and amino nitrogen being respectively 1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of
total nitrogen), The polyenzyme method was used to make 14.8% protein gravy fromHarengula zunasi offal. In addition, inorganic elements, the phosphorus content is the highest.
This project was aided financially by the Guangdong Science and Technology Office (No. 2KM06002S). 相似文献
17.
A new enzyme (alkaline protease 894) obtained from the marine extremophile Flavobacterium yellowsea (YS-80-122) has exhibited strong substrate-binding and catalytic activity, even at low temperature, but the characteristics of the hydrolysis with this enzyme are still unclear. The pearl oyster Pinctada martensii was used in this study as the raw material to illustrate the kinetic properties of protease 894. After investigating the intrinsic relationship between the degree of hydrolysis and several factors, including initial reaction pH, temperature, substrate concentration, enzyme concentration, and hydrolysis time, the kinetics model was established. This study showed that the optimal conditions for the enzymatic hydrolysis were an initial reaction pH of 5.0, temperature of 30°C, substrate concentration of 10% (w/v), enzyme concentration of 2 500 U/g, and hydrolysis time of 160 min. The kinetic characteristics of the protease for the hydrolysis of P. martensii were obtained. The inactivation constant was found to be 15.16/min, and the average relative error between the derived kinetics model and the actual measurement was only 3.04%, which indicated a high degree of fitness. Therefore, this study provides a basis for the investigation of the concrete kinetic characteristics of the new protease, which has potential applications in the food industry. 相似文献
18.
Protease is wildly used in various fields, such as food, medicine, washing, leather, cosmetics and other industrial fields. In this study, an alkaline protease secreted by Micrococcus NH54PC02 isolated from the South China Sea was purified and characterized. The growth curve and enzyme activity curve indicated that the cell reached a maximum concentration at the 30th hour and the enzyme activity reached the maximum value at the 36th hour. The protease was purified with 3 steps involving ammonium sulfate precipitation, ion-exchange chromatography and hydrophobic chromatography with 8.22-fold increase in specific activity and 23.68% increase in the recovery. The molecular mass of the protease was estimated to be 25 kDa by SDS-PAGE analysis. The optimum temperature and pH for the protease activity were 50°C and pH 10.0, respectively. The protease showed a strong stability in a wide range of pH values ranging from 6.0–11.0, and maintained 90% enzyme activity in strong alkaline environment with pH 11.0. Inhibitor trials indicated that the protease might be serine protease. But it also possessed the characteristic of metalloprotease as it could be strongly inhibited by EDTA and strongly stimulated by Mn2+. Evaluation of matrix-assisted laser desorption ionization/time-of-flight MS (MALDI-TOF-TOF/MS) showed that the protease might belong to the peptidase S8 family. 相似文献