共查询到18条相似文献，搜索用时 156 毫秒
1.
以麸皮、豆粕和红糖为基料,固态培养沼泽生红冬孢酵母(Rhodosporidium paludigenum),研究沼泽生红冬孢酵母的固体培养条件和培养基。结果表明,优化的固体培养基和培养条件为:豆粕、麸皮和红糖的最佳质量比为3∶1∶1;每10 g基料,各组分添加量分别为酵母膏0.2 g,硫酸铵0.2 g,水12 mL,接种1.5 mL菌液;培养基厚度2.35 cm。在此条件下培养60 h,红酵母的细胞产量最高达101.9×108g-1。 相似文献
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研究了米曲霉中性蛋白酶的特性。结果表明,米曲霉中性蛋白酶的最佳提取方法为加入pH 7.2的缓冲液研匀,置恒温(30℃)培养箱中提取30 min,每10 min搅动1次,最后用4层纱布过滤。酶反应最适温度为55℃,最适pH值为7.2。NaCl和EDTA对其有一定的抑制作用。酶的热稳定性好,在45℃的温度下处理24 h后,酶活力仍有45.5%。米曲霉中性蛋白酶与培养物一起保存时,稳定性也较好,在4℃保存一个月后,酶活力几乎无变化。 相似文献
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为研究生物酶采油解堵剂中产蛋白酶菌株的初、复筛选及培养条件优化,从大庆原油样品中筛选菌种,通过水解酪素的透明圈实验及福林酚测蛋白酶酶活的方法进行菌株的初、复筛选;以蛋白酶酶活为优化指标,采用单因素实验对筛选的产蛋白酶菌株的培养基及培养条件进行优化,优化最适培养基:可溶性淀粉为15g/L,蛋白胨为20g/L,酵母膏为20g/L,NaCl为1.0g/L,CaCl2为0.02g/L,Na2HPO4为0.2g/L,NaH2PO4为0.1g/L;在初始pH为6.0、接种量为5%(体积分数)、温度为31℃、摇床转速为160r/min的条件下,培养72h后,菌株的蛋白酶酶活为551.0U/mL,为复筛选菌株的蛋白酶酶活的22.92倍,即为菌株生长繁殖及代谢的最佳条件,能够获得更高的蛋白酶酶活,有利于后续实验的进行.结果表明:菌株产蛋白酶对原油作用效果为发酵液表面张力从作用前的56.2mN/m降低到作用后的30.5mN/m,表面张力显著降低,还有降解降黏原油等效果,具有一定的研究价值. 相似文献
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利用米曲霉把黄芩苷转化为黄芩素。转化条件为:液体发酵,pH6,30℃,利用综合马铃薯培养基与玉米芯培养基两步发酵。菌种采用两步活化法,将经综合马铃薯培养基培养48 h的米曲霉加入至玉米芯培养基培养48 h后,再加入底物黄芩苷继续培养3 d。玉米芯发酵液加甲醇混匀后过滤,通过高效液相色谱检测,结果表明米曲霉已经将黄芩苷转化为黄芩素,转化率为40.72%。同时用乙醚萃取法从玉米芯发酵液中萃取到黄色固体提取物,经过高效液相色谱与标准品对照鉴定,该提取物为黄芩素。 相似文献
6.
米曲霉中性蛋白酶特性的研究 总被引：2，自引：0，他引：2
研究了米曲霉中性蛋白酶的特性。结果表明，米曲霉中性蛋白酶的最佳提取方法为加入pH7．2的缓冲液研匀，置恒温（30℃）培养箱中提取30min．每10min搅动1次，最后用4层纱布过滤。酶反应最适温度为55℃，最适pH值为7．2。NaCl和EDTA对其有一定的抑制作用。酶的热稳定性好．在45℃的温度下处理24h后，酶活力仍有45．5％。米曲霉中性蛋白酶与培养物一起保存时，稳定性也较好，在4℃保存一个月后，酶活力几乎无变化。 相似文献
7.
利用米曲霉把黄芩苷转化为黄芩素。转化条件为：液体发酵，pH6，30℃，利用综合马铃薯培养基与玉米芯培养基两步发酵。菌种采用两步活化法，将经综合马铃薯培养基培养48h的米曲霉加入至玉米芯培养基培养48h后，再加入底物黄芩苷继续培养3d。玉米芯发酵液加甲醇混匀后过滤，通过高效液相色谱检测，结果表明米曲霉已经将黄芩苷转化为黄芩素，转化率为40．72％。同时用乙醚萃取法从玉米芯发酵液中萃取到黄色固体提取物，经过高效液相色谱与标准品对照鉴定，该提取物为黄芩素。 相似文献
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从甲壳素出发,先制备了3种不同脱乙酰度的壳聚糖,选择过硫酸铵_亚硫酸氢钠为氧化还原体系引发剂,合成了壳聚糖接枝丙烯酰胺聚合物;通过红外光谱,电镜扫描对聚合物进行结构表征,结果表明壳聚糖结构发生了变化,通过在相同条件下比较壳聚糖不同脱乙酰度对接枝率的影响,得出了在脱乙酰度为68.5%时聚合物的接枝率最大为250%,其最佳合成工艺为∶m(壳聚糖)∶m(丙烯酰胺)=1∶5,引发剂用量为体系质量分数的0.3%~0.4%,温度55~60℃,反应时间为2.5~3.0 h。 相似文献
10.
为探索石油污染土壤的高效修复方法,从实验室保存的优势菌中筛选得到4株降油效果最佳菌,采用摇床和恒温培养箱培养,对含油量为5%的石油污染土壤进行微生物菌剂强化处理和环境强化实验.微生物菌剂强化结果表明:4种菌和除油效果最好的A、C、D混合菌3 d可将石油烃依次降解24%、19.81%、22.55%、26.46%、39.67%;并对该菌群的最佳投加配比进行确定,A、C、D菌群数量的最佳配比为NA∶NC∶ND=1∶2∶0.5,3 d内菌群A、C、D在最佳接种配比情况下可将石油烃降解44.2%.环境强化实验结果表明:A、C、D菌群在最佳修复条件营养物质C∶N∶P为75∶8∶3、表面活性剂为0.5%、通气条件为6层纱布、电子受体H2O2的加入量为1.5%下,3 d内石油烃降解61.46%,比自然条件下修复的除油率4.7%提高了56.76%,较只进行菌种强化时最高除油率44.2%提高了约17%. 相似文献
11.
The marine yeast strain W6b isolated from sediment of the South China Sea was found to produce a cell-bound acid protease.
The crude acid protease produced by this marine yeast showed the highest activity at pH 3.5 and 40 °C. The optimal pH and
temperature for the crude acid protease were in agreement with those for acid protease produced by the terrestrial yeasts.
The optimal medium of the acid protease production was seawater containing 1.0% glucose, 1.5% casein, and 0.5% yeast extract,
and the optimal cultivation conditions of the acid protease production were pH 4.0, a temperature of 25 °C and a shaking speed
of 140 rmin−1. Under the optimal conditions, 72.5 UmL−1 of acid protease activity could be obtained in cell suspension within 48 h of fermentation at shake flask level. The acid
protease production was induced by high-molecular-weight nitrogen sources and repressed by low-molecular-weight nitrogen sources.
Skimmed-milk-clotting test showed that the crude acid protease from the cell suspension of the yeast W6b had high skimmed
milk coagulability. The acid protease produced by M. reukaufii W6b may have highly potential applications in cheese, food and fermentation industries. 相似文献
12.
WANG Ping CHI Zhenming MA Chunling 《中国海洋大学学报(英文版)》2006,5(3):263-268
Yeast strain 10 with high yield of protease was isolated from sediments of saltern near Qingdao, China. The protease had the highest activity at pH 9.0 and 45℃. The optimal medium for the maximum alkaline protease production of strain 10 was 2.5 g soluble starch and 2.0 g NaNO3 in 100 mL seawater with initial pH6.0. The optimal cultivation conditions for the maximum protease production were temperature 24.5 ℃, aeration rate 8.0 L min^- 1 and agitation speed 150 r min^-1 . Under the optimal conditions, 623.1 Umg^-1 protein of alkaline protease was reached in the culture within 30 h of fermentation. 相似文献
13.
Lactic acid bacteria (LAB) were encapsulated with alginate, gelatin and trehalose additives by the extrusion method and dried at 4 ℃. The microcapsules were generally spherical and had a wrinkled surface with a size of 1.7 mm ± 0.2 mm. Trehalose as a carbohydrate source in the culture medium could reduce acid production and performed no function in the positive proliferation of LAB. Using trehalose as a carbohydrate source and protective medium simultaneously had a benefit in the protection of LAB cells dur... 相似文献
14.
The new polyenzyme method for making gravy fromHarengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal
trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for
the polyenzyme method were pH of 7.0, temperature of 50°C, alkaline and neutral protease concentration of 1.5% respectively,
hydrolysis time of 120 min, and flavorase concentration of 2.0%, for 60 min. The new gravy-making technology yields a nutritious
and delicious gravy containing 40.3% of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and
Ser comprising 49.5%, total and amino nitrogen being respectively 1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of
total nitrogen), The polyenzyme method was used to make 14.8% protein gravy fromHarengula zunasi offal. In addition, inorganic elements, the phosphorus content is the highest.
This project was aided financially by the Guangdong Science and Technology Office (No. 2KM06002S). 相似文献
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The new polyenzyme method for making gravy from Harengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50℃ , alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, andflavorase concentration of 2.0% , for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3 % of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5% , total and amino nitrogen being respectively1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy from Harengula zunasi offal. In addition, in inorganic elements, the phosphorus content is the highest. 相似文献
16.
A Gram negative bacterium Ar/W/b/75°25＇N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25＇N, and longitude 162°25＇W in Chukchi sea, Arctic. The strain can grow at the temperature range from 7℃ to 30℃, and grow better at 30(℃. It can not grow at 40℃. Keeping certain salinity concentration in medium is necessary for cell growth. It grows well in medium containing salinity concentration from 0. 5 % to 10 % sodium chloride. Glucose, sucrose and soluble starch can be utilized by the strain, among which glucose is the optimal carbon source. Peptone is the optimal organic nitrogen source for cell growth and protease producing, and ammonium nitrate is the optimal inorganic nitrogen source.About 75.7% of total protease of the strain are extracellular enzyme. Optimal temperature for proteolytic activity is at 40℃. Protease of the strain keeps stable below 40℃, and shows high proteolytic activity within the pH range from 7 to 11. 相似文献
17.
酶解法提取鱼油的工艺参数优化 总被引：1，自引：0，他引：1
利用蛋白酶酶解法从黄鳍金枪鱼(Thunnus albacares)加工的下脚料———鱼头中提取鱼油。以鱼油提取率和感官特征为指标,通过正交优化实验设计,获得了胰蛋白酶提取鱼油的最佳酶解工艺参数:酶解温度45℃,酶添加量1.5%,料液质量比1∶1,酶解时间4 h,酶解pH 8。在该条件下,鱼油的提取率为4.34%,理化指标除过氧化值外均达到SC/T3502-2000的粗鱼油二级标准,多不饱和脂肪酸总含量高达38.47%,其中DHA和EPA的含量分别为23.63%和4.84%。 相似文献
18.
A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15℃ and 30℃, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35 ℃ and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease. The presence of Ca2+ and Mn2+ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid (AA) residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family. 相似文献