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以植物组织培养技术培养的水花生愈伤组织为实验材料,研究了过量Zn2+(0、0.2、0.4、0.8、1.6 mmol/L)对水花生愈伤组织的氧化损伤及多胺代谢的影响.结果表明,随着Zn2+浓度增加:(1)水花生愈伤组织的超氧阴离子产生速率和硫代巴比妥酸反应物水平显著提升,超氧化物歧化酶活性明显抑制,叶绿素a含量显著降低;(2)精氨酸脱羧酶和鸟氨酸脱羧酶活性均升高,多胺氧化酶和二胺氧化酶活性在低Zn2+浓度处升高、高Zn2+浓度处降低;(3)总腐胺和总精胺含量在低Zn2+浓度处降低、高Zn2+浓度处增加,总亚精胺含量仅在Zn2+浓度为1.6 mmol/L时有显著增加;(4)游离态腐胺含量变化与总腐胺含量相似,游离态亚精胺含量在高Zn2+浓度时下降,游离态精胺含量仅在Zn2+浓度为0.2 mmol/L时略有下降;(5)结合态腐胺和精胺在Zn2+浓度为0.2 mmol/L时下降而在其余处理组中上升,结合态亚精胺含量各处理组中均增加;(6)束缚态腐胺和亚精胺含量均下降.可见,过量Zn2+胁迫导致水花生愈伤组织氧化性损伤,并扰乱其多胺代谢平衡;束缚态多胺向结合态多胺的转化是过量Zn2+胁迫下水花生愈伤组织多胺代谢变化的一个显著特征;以结合态为代表的内源性多胺积累对水花生愈伤组织抵抗过量Zn2+胁迫具有重要意义. 相似文献
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以菹草(Potamogeton crispus L.)无菌苗为实验材料,研究其在不同浓度汞(Hg2+)的胁迫下,光合色素含量、叶绿体自发荧光强度、ATP酶活性及相关矿质营养元素(Ca、Mg、Na、K、Fe)的含量变化以及可溶性蛋白含量的变化,以探讨Hg2+胁迫对菹草无菌苗光合系统及相关生理指标变化的影响.研究表明:随着Hg2+浓度的增加,各种光合色素的含量均呈现下降的趋势,叶绿体自发荧光强度逐渐减弱;Na/K逐渐增大,Na+ K+-ATP酶活性逐渐降低,Ca2+ Mg2+-ATP酶活性呈先升后降趋势;可溶性蛋白含量先升后降.结果表明,菹草无菌苗在Hg2+胁迫下,光合及其它正常的生理过程遭到破坏,功能紊乱. 相似文献
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本文以组织培养技术培养的菹草无菌苗为实验材料,研究了2 mmol/L外源鸟氨酸(L-Orn)对40μmol/L镉(Cd)胁迫下菹草体内Cd含量、光合色素水平、超氧阴离子(O·-2)产生速率、过氧化氢(H2O2)、丙二醛(MDA)与可溶性蛋白含量以及脯氨酸和多胺代谢的影响.结果显示:(1)在单一Cd胁迫下,菹草体内的Cd大量积累,达到335.00μg/g,并诱导了明显的氧化胁迫,具体表现为O·-2产生速率以及H2O2和MDA含量显著升高,可溶性蛋白以及光合色素含量明显下降.外源L-Orn的添加很大程度上抑制了菹草体内Cd的积累,Cd含量降至232.50μg/g,并降低了活性氧(ROS)水平,减轻了膜脂过氧化程度,减缓了光合色素和可溶性蛋白的分解.(2)在单一Cd胁迫下,伴随着吡咯啉-5-羧酸合成酶(P5CS)活性的增加和脯氨酸脱氢酶(ProDH)活性的下降,菹草体内的脯氨酸含量轻微增加,仅为对照组的1.91倍,而外源L-Orn显著增强了P5CS和δ-鸟氨酸转氨酶(OAT)的活性,使脯氨酸含量大幅度升高,为对照组的8.36倍.(3)单一Cd胁迫分别通过激活精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)和多胺氧化酶(PAO)的活性,使腐胺(Put)的含量增加,亚精胺(Spd)和精胺(Spm)含量减少.而施用外源L-Orn后,ADC和ODC的活性进一步增强,Put的含量大幅增加,并促进Put向Spd和Spm的转化,同时PAO的活性下降,使Spd和Spm含量增加,从而使总多胺含量增加.由此可见,外施L-Orn可通过抑制Cd的进入和参与调节脯氨酸以及多胺代谢,抑制ROS的积累、膜脂过氧化、光合色素以及蛋白质的分解,一定程度上增强菹草对Cd胁迫的抗性. 相似文献
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碱度增加对蛋白核小球藻光合活性与胞外多糖的影响 总被引：1，自引：0，他引：1
本文研究了不同重碳酸盐(HCO3)碱度2.3mmol/L(ALK2.3)和12.4mmoFL(ALK12.4)条件对蛋白核小球藻光合活性、色素组成、丙二醛(MDA)含量与胞外多糖的影响.实验结果表明,碱度增加对蛋白核小球藻光合活性呈促进一抑制一促进效应,ALK2.3对光合活性影响的强度.高于ALK12.4.碱度增加提高叶绿素b/叶绿素a(Chl.b/Chl.a)的值,降低类胡萝卜素/叶绿素(Caro/TChl)的值,并且ALKl24条件下对藻细胞的作用程度强于ALK2.3.此外碱度增加刺激蛋白核小球藻胞外多糖分泌,ALK2.3在培养初期提高MDA含量,ALK12.4下细胞MDA含量显著降低.说明碱度增加会促进蛋白核小球藻光合活性,促进光合产物的积累与分泌.暗示胞外多糖的分泌可能是细胞适应高碱度的一种自我保护机制. 相似文献
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水深与水体的多个环境要素(如光照、温度等)密切关联.因而水深会影响沉水植物的生长、繁殖和生理生化特征,包括叶片的光合色素组成和C、N、P化学计量特征.本研究通过对洱海4种沉水植物苦草(Vallisneria natans)、微齿眼子菜(Potamogeton maackianus)、光叶眼子菜(Potamogeton lucens)和篦齿眼子菜(Potamogeton pectinatus)在不同水深梯度的叶片色素组成和C、N、P化学计量特征进行分析,以阐明水深对这些指标的影响规律和机制.结果表明:水深对4种沉水植物叶片光合色素组成和C、N、P化学计量特征的影响具有种间差异性;苦草、微齿眼子菜和光叶眼子菜叶片N含量,苦草和光叶眼子菜叶片P含量都与叶片叶绿素含量呈显著正相关;苦草叶片N含量随水深增加而增加,可能是由苦草为应对深水弱光胁迫而加强光合色素和蛋白的合成导致的,而磷含量的增加可能还受其他因素的影响. 相似文献
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采用营养液水培的方法,研究不同镍(Ni)添加浓度(0、0.05、0.10、0.15、0.20 mmol/L)对水鳖(Hydrocharis dubia)叶片中Ni元素积累、营养元素(Ca、Mg、K、Fe和Mn)含量、氧化胁迫和抗氧化系统的影响.结果显示:(1)叶片中Ni含量以浓度依赖的方式增加;(2)叶片中营养元素含量(Ca、Mg、K、Fe、Mn)也受影响,其中Ca、Mg含量随Ni浓度增加而显著上升;K含量随Ni浓度增加有所升高,但不显著;Mn含量虽在较大Ni浓度时有所下降,但处理组始终高于对照组;而Fe含量则随Ni浓度增加而下降;(3)Ni胁迫诱导了明显的氧化胁迫,主要表现为:O·-2产生速率以及过氧化氢和丙二醛含量显著升高,可溶性蛋白以及光合色素含量明显下降;(4)随着Ni浓度的升高,过氧化物酶活性逐渐升高,过氧化氢酶活性呈下降趋势,超氧化物歧化酶活性在Ni添加浓度为0.05 mmol/L时出现短暂升高随后下降;维生素E和黄酮含量随Ni浓度增加呈缓慢上升趋势;还原型谷胱甘肽未发生明显变化,非蛋白巯基和植物络合素含量均表现为先升后降的趋势;总抗氧化能力随Ni浓度升高先增强后减弱.由此可见,Ni添加引起水鳖叶片中Ni元素的累积和营养元素含量变化,同时产生明显的氧化胁迫,打破了抗氧化系统的平衡. 相似文献
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我国水华蓝藻的新类群——阿氏浮丝藻(Planktothrix agardhii)生理特性 总被引：3，自引：2，他引：1
阿氏浮丝藻(Planktothrix agardhii)是水华蓝藻的重要类群,实验对我国不同省份分离到的5株阿氏浮丝藻生长速率、色素组成、光合活性参数进行了测定.结果表明分离于广州的HABll28叶绿素a(Chla)含量较低,藻蓝蛋白(c-Pc)比例和含量较高.电子传递速率(ETR)测定结果表明,HABIl28的ETR曲线较高,最大ETRme值也显著高于其余4株,高效的光能电子传递链弥补了光合色素Chla的不足,以致HABIl28保持了中性的生长速率.分离于武汉东湖的HAB631则与之正好相反,较高的ChLa受限于光能电子传递链的薄弱,生长速率表现较低.分离于北京、上海、昆明的3株藻株在各项生理指标上无显著差异. 相似文献
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研究了不同浓度Hg2 +对水鳖叶片光合放氧速率、呼吸速率、细胞膜透性、硝酸还原酶活性以及超微结构的毒害影响 .结果表明 ,随着Hg2 +浓度的增加 ,水鳖叶片的光合放氧速率逐渐下降 ,呼吸速率、硝酸还原酶活性先升后降 ,而细胞膜透性则逐渐上升 .电镜观察发现 ,水鳖叶细胞遭受Hg2 +毒害初期 ,染色质凝集 ,叶绿体中类囊体片层以及线粒体嵴突膨胀 .随着叶细胞遭受毒害程度的加深 ,核膜破裂 ,染色质与核质消失 ,叶绿体膨胀解体 ,线粒体空泡化 相似文献
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以组织培养的菹草无菌苗为实验材料,研究了外源Ca(NO3)2的添加对汞(Hg)胁迫下菹草体内Hg积累、矿质元素、光合色素、活性氧、丙二醛(MDA)、维生素C(VC)、维生素E(VE)、谷胱甘肽(GSH)以及多胺(PAs)代谢产生的影响效应机制.结果表明:(1)Hg胁迫下,菹草体内Hg大量积累,达165.79μg/g(FW),并伴随矿质元素P、K、Fe、Mg和Na含量的明显降低.外源Ca(NO3)2的添加很大程度上可抑制菹草体内Hg积累,使其降至110.15μg/g(FW),并减轻Hg胁迫所造成的元素失衡;(2)Hg胁迫使菹草体内光合色素含量及叶绿素a/b值大幅降低,并诱导积累大量超氧阴离子、过氧化氢及MDA,同时VC、VE和GSH含量显著提升.外源Ca(NO3)2的添加则减缓了菹草体内光合色素的分解,降低活性氧水平,减轻了膜脂过氧化程度,更使VC、VE及GSH含量在一定程度上恢复到正常水平;(3)Hg胁迫下,伴随着多胺氧化酶、二胺氧化酶和精氨酸脱羧酶活性的显著提升以及鸟氨酸脱羧酶活性的降低,导致菹草体内腐胺(Put)、精胺(Spm)含量明显降低,亚精胺(Spd)含量及游离态(Spd+Spm)/Put比值轻微增加.而外源Ca(NO3)2添加后,菹草通过调节体内PAs代谢酶活性,改变了Hg胁迫下PAs含量变化,主要表现为Put、Spm含量回升显著,游离态(Spd+Spm)/Put比值进一步升高.综上所述,外源Ca的添加可抑制Hg的吸收并参与调节多胺代谢,通过内源多胺含量的改变抑制了植物体内活性氧的积累、膜脂过氧化、光合色素及抗氧化物质的分解,增强菹草无菌苗对重金属Hg的耐受力,对减轻植物Hg胁迫有重要作用. 相似文献
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太湖人工生态系统中氮循环细菌分布 总被引：7，自引：1，他引：6
对太湖五里湖的以水生高等植物为主的湖泊人工生态系统,用最大可能数（ＭＰＮ）法,测定了该系统内各种生态类型水生高等植物群落内的４类氮循环细菌的分布．结果表明,反硝化细菌的最大可能数（ＭＰＮ）,在水生高等植物群落内水体中较敞水区湖水高２－５个数量级,差异极显著（Ｐ＜０．０１）,漂浮植物群落内水体的反硝化细菌ＭＰＮ值较沉水和浮叶植物群落内水体高２－３个数量级,差异极显著（Ｐ＜０．０１）;硝化细菌ＭＰＮ值,敞水区湖水高于凤眼莲、水花生群落内水体,差异显著（ Ｐ＜ ０． ０５）,菱群落与其他群落比较,亦有极显著性差异（ Ｐ＜ ０． ０１）;亚硝化细菌ＭＰＮ值,在水生高等植物群落内的水体中较敞水区高 ３－ ４个数量级,差异极显著（ Ｐ＜ ０． ０１） ;氨化细菌ＭＰＮ值,在水生高等植物群落内的水体中高于敞水区水体．除硝化细菌外,反硝化、亚硝化及氨化细菌均在根际处最为密集,且由根际向外呈现递减趋势． 相似文献
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Blagoi G. Amov 《Earth and Planetary Science Letters》1983,65(2):311-321
On the basis of a dynamic model of a continuous Pb isotope evolution, the variations in the isotopic ratios of uranogenic and thorogenic Pb in a number of young ore deposits, ocean sediments and volcanic rocks from mid-ocean ridges, oceanic islands, island arcs and continents are evaluated. The deviations in the Th/U ratio from its model values are calculated. A model parameterT which indicates a more ancient enrichment in U/Pb (crustal-type Pb sources) or younger enrichment in U/Pb (mantle-type Pb sources) is also calculated. The relative change in the Th/U ratio is close to 1 for mantle-type Pb sources (lowerT values) and decreases for crustal-type Pb sources (higherT values). An interpretation of these changes could be connected with the differences in the U and Th geochemical behaviours in metamorphic processes under the conditions of a deeper (mantle) or crustal source owing to the differences in their valence states. In some cases they have similar (U4+ and Th4+) migration abilities; in others (U6+ and Th4+), considerably different. 相似文献
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Jianfan Sun Qaiser Javed Ahmad Azeem Muhammad Saif Ullah Ghulam Rasool Daolin Du 《洁净——土壤、空气、水》2020,48(9)
Coordination of plant functional traits with changes in the environment is helpful to understand the mechanisms underlying both invasiveness and adaptation of plants. Thus, to investigate the performance and functional traits in invasive Alternanthera philoxeroides (Mart.), an experiment of water stress is conducted with different nutrient concentrations. Alternanthera philoxeroides plants are grown under natural and nutrient soils and subjected to three levels of nitrogen (N) and phosphorus (P) solution: ambient P and N concentration (P?N?), P addition with an ambient N concentration (P+N?), and P addition with high N concentration (P+N+) in combination with three different irrigation water levels as 1) 100% irrigation, 2) 50% irrigation, and 3) 25% deficit irrigation. Based on results, A. philoxeroides produces significantly higher biomass in both soils under 100% irrigation with P+N? treatment and exhibits higher values of leaf area and root length. However, 25% irrigation with P?N? treatment in both soils exerts a significant negative effect on relative growth rate and root/shoot ratio of A. philoxeroides plants. Under 50% irrigation in soils with both P+N? and P+N+ treatments, high values of leaf nitrogen are recorded. Moreover, nutrient soil is more supportive to A. philoxeroides than natural soil. 相似文献
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Abstract Geochemical and Sr–Nd–Pb isotope characteristics, as well as K–Ar geochronology of a massive pitchstone (volcanic glass) stock erupted into Late Cretaceous lapilli tuff and rhyolite in the Gohado area, southwestern Okcheon Belt, South Korea, are reported. The pitchstones are highly evolved with SiO2 contents ranging from ～72 to 73 wt%, K2O/Na2O ratios of 1.04–1.23 and low MgO/FeOt values (0.17–0.20). The pitchstones are weakly peraluminous and the ASI (molar Al2O3/Na2O + K2O + CaO) values are significantly lower than 1.1. The pitchstones also display a general calc‐alkaline nature with significant alkali contents. The rare earth elements (REE) compositions show moderately fractionated nature with (La/Yb)N ranging from 11 to 16. Chondrite normalized REE patterns show relative enrichment of light REE over heavy REE and moderate Eu anomaly (Eu/Eu* ratio varies from 0.53 to 0.57). A distinct negative Nb anomaly is observed for all pitchstones on a primitive mantle normalized trace element diagram, typical of subduction‐related magmatism and crustal‐derived granites. All these features are characteristic of I‐type granites derived from a continental arc. The pitchstones have Zr contents of 98.5–103.5 ppm with zircon thermometry yielding temperatures of 749–755°C (mean 752°C). The K–Ar analyses of representative pitchstone samples yielded ages of 58.7 ± 2.3 and 62.4 ± 2.1 Ma with a mean age of 61 Ma. The rocks show nearly uniform initial 87Sr/86Sr isotopic ratios of 0.7104–0.7106 and identical 143Nd/144Nd initial ratio of 0.5120. The rocks display negative εNd (61 Ma) values of ?12. The depleted mantle model ages (TDM) range from 1.54 Ga to 1.57 Ga. The Pb isotope ratios are 206Pb/204Pb = 18.522–18.552, 207Pb/204Pb = 15.642–15.680 and 208Pb/204Pb = 38.794–38.923. These ratios suggest that the Gohado pitchstones were formed in a continental arc environment by partial melting of a 1.54 Ga to 1.57 Ga parental sources of lower crustal rocks probably of mafic or intermediate compositions. 相似文献
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巢湖流域水生植物多样性 总被引：2，自引：1，他引：1
水生植物在浅水湖泊或河流生态系统中具有非常重要的作用.通过2次野外实地调查,对巢湖流域水生植物物种的区系组成和多样性进行初步分析.结果表明:巢湖流域水生植物共有43科85属123种(含种下分类单位,下同),其中蕨类植物有5科6属6种,被子植物38科77属117种,在被子植物中双子叶植物25科39属63种,单子叶植物13科40属54种.水禾(Hygroryza aristata)为安徽省首次记录;以禾本科(Gramineae)、蓼科(Polygonaceae)和莎草科(Cyperaceae)等为优势科;以蓼属(Polygonum)、眼子菜属(Potamogeton)和菱属(Trapa)等为优势属,属的组成比较分散,寡种属和单种属占总属数的96.5%;以喜旱莲子草(Alternanthera philoxeroides)、芦苇(Phragmites australis)、菹草(Potamogeton crispus)、菱(Trapa sp.)、金鱼藻(Ceratophyllum demersum)和黑藻(Hydrilla verticillata)等为优势种;从分布区类型看,在科级水平上有6个类型,科的分布区类型以世界广布最多,达55.81%,热带分布的科(11科)多于温带分布的科(8科);在属级水平上有13个类型,属的分布区类型以世界广布最多(36.47%),其次是泛热带分布(20.0%);从不同流域来看,水生植物物种多样性以白石天河流域最高,店埠河南淝河流域最低,这可能与南淝河水质污染比较严重有一定的关系. 相似文献
18.
This paper presents new zircon U–Pb geochronological, Hf isotopic and whole-rock geochemical data for the granitic plutons in the Xing'an Massif, Northeast China, to constrain the Late Mesozoic tectonic evolution of the Mongol-Okhotsk Ocean and the Paleo-Pacific Ocean. The zircon U–Pb ages indicate that the granitoids emplaced during the Late Jurassic–Early Cretaceous. The granodiorites show an adakitic affinity with high Sr/Y ratios and low Yb (< 1.30 μg/g) contents. The monzogranites exhibit high SiO2, low MgO contents, enrichment in LILEs (Rb, K, and Th), and depletion in HSFEs (Ta, Nb, Zr, P, and Ti). Petrological and geochemical features of these monzogranites suggest that they are highly fractionated I-type granitoids. In addition, the zircon εHf(t) values and two-stage model ages (TDM2 ) are in the range of +2.6 to +8.1 and 669–1011 Ma, respectively, indicating that primary magma was generated by partial melting of juvenile lower-crustal materials, and there was a significant crustal growth in the Phanerozoic in the Northeast China. Combined with the coeval granitoids widely exposed in the Xing'an Massif, we conclude that the Late Jurassic magma in Northeast China was generated in an extensional setting related to the closure of the Mongol-Okhotsk Ocean, but the Early Cretaceous magma was related to the subduction of the Paleo-Pacific Plate. 相似文献