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1.
报道了勒氏笛鲷外周血细胞的显微结构,血涂片经过Wright染色液染色,可区分出红血细胞、血栓细胞、嗜中性粒细胞、单核细胞、淋巴细胞等五种血细胞,没有发现嗜碱性粒细胞、嗜酸性粒细胞.白血细胞中,血栓细胞体积最小,单核细胞体积最大;血栓细胞数目最多,淋巴细胞和单核细胞数目最少;淋巴细胞有大小两种类型大淋巴细胞和小淋巴细胞.此外,在外周血液中还观察到少量未成熟的红细胞和嗜中性粒细胞,偶而可见正在分裂的红细胞,提示红细胞也可在外周血直接分裂.  相似文献   

2.
勒氏笛鲷外周血细胞显微结构的观察   总被引:5,自引:1,他引:4  
报道了勒氏笛鲷外周血细胞的显微结构,血涂片经过Wright染色液染色,可区分出:红细胞、血栓细胞、嗜中性粒细胞,单核细胞,淋巴细胞等五种血细胞,没有发现嗜碱性粒细胞,嗜酸性粒细胞,白血细胞中,血栓细胞体积最小,单核细胞体积最大;血栓细胞数目最多,淋巴细胞和单核细胞数目最少;淋巴细胞有大小两种类型;大淋巴细胞和小淋巴细胞,外外,在外周血液中还观察到少量未成熟的红细胞和嗜中性粒细胞,偶而可见正在分裂的红细胞,提示红细胞也可在外周血直接分裂。  相似文献   

3.
报道了乌龟血细胞的显微结构。血涂片经过染色,可鉴定出红细胞、单核细胞、淋巴细胞、血栓细胞、嗜酸性粒细胞、嗜碱性粒细胞、嗜中性粒细胞等7 种细胞。红细胞数量多,长椭圆形,具圆核。白细胞数量少,圆形,分为两种:无粒型白细胞和粒型白细胞。无粒型白细胞包括单核细胞和淋巴细胞,无特殊颗粒;粒型白细胞包括嗜酸性粒细胞、嗜碱性粒细胞和嗜中性粒细胞,有特殊颗粒。  相似文献   

4.
乌龟外周血细胞的显微结构   总被引:3,自引:0,他引:3  
报道了乌龟血细胞的显微结构。血涂片经过染色,可鉴定出红细胞、单核细胞、淋巴细胞、血栓细胞、嗜酸性粒细胞、嗜碱性粒细胞、嗜中性粒细胞等7种细胞。红细胞数量多,长椭圆形,具圆核。白细胞数量少,圆形,分为两种:无粒型白细胞和粒型白细胞。无粒型白细胞包括单核细胞和淋巴细胞,无特殊颗粒;粒型白细胞包括嗜酸性粒细胞、嗜碱性粒细胞和嗜中性粒细胞,有特殊颗粒。  相似文献   

5.
对多鳞鱚(Sillago sihama Forskal)Wright染色的血涂片进行显微观察。结果表明多鳞鱚外周血细胞可区分为红细胞、血栓细胞、淋巴细胞、嗜中性粒细胞、嗜酸性粒细胞和单核细胞等6种细胞,未发现嗜碱性粒细胞。血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞在白细胞中所占比例分别为47.5%、32.5%、12.5%和7.5%,嗜酸性粒细胞在整个实验中只发现7个。并发现较多未成熟的红细胞以及少量正在分裂的红细胞和晚幼嗜中性粒细胞。  相似文献   

6.
对多鳞鱚(Sillago sihama Forskál)Wright染色的血涂片进行显微观察。结果表明多鳞鱚外周血细胞可区分为红细胞、血栓细胞、淋巴细胞、嗜中性粒细胞、嗜酸性粒细胞和单核细胞等6种细胞,未发现嗜碱性粒细胞。血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞在白细胞中所占比例分别为47.5%、32.5%、12.5%和7.5%,嗜酸性粒细胞在整个实验中只发现7个。并发现较多未成熟的红细胞以及少量正在分裂的红细胞和晚幼嗜中性粒细胞。  相似文献   

7.
以全鱼粉作为唯一蛋白源(D1),用豆粕替代10%、20%鱼粉(D2、D3),玉米蛋白粉替代10%鱼粉(D4),啤酒酵母替代10%鱼粉(D5),配制5组等氮等能饲料,每种饲料设置3个实验组,进行56 d的养殖实验。通过血液和组织涂(印)片、细胞染色和显微观察,研究人工培育的褐点石斑鱼幼鱼外周血液白细胞的分类组成,头肾、脾脏、体肾和肝脏等4种组织中各类血细胞的发生情况,以及不同蛋白源饲料对褐点石斑鱼血细胞发生的影响。结果表明:褐点石斑鱼外周血液中的白细胞由淋巴细胞(53.30%±4.66%)、血栓细胞(35.69%±3.85%)、嗜中性粒细胞(10.34%±3.14%)、单核细胞(0.28%±0.36%)、浆细胞(0.24%±0.34%)和嗜酸性粒细胞(0.15%±0.27%)组成;组织印迹片中,未成熟的红细胞、淋巴细胞和粒细胞主要在头肾印迹片中出现,未成熟的单核细胞主要在头肾和脾脏印迹片中出现,血栓细胞在肝脏印迹片中数量最多,推断褐点石斑鱼幼鱼主要的造血组织是头肾,其次是脾脏;在4种组织中均观察到浆细胞,在体肾印迹片中观察到嗜碱性粒细胞,在肝脏印迹片中观察到巨噬细胞,在头肾印迹片中还观察到巨大原红细胞。显微观察和数据统计分析的结果都表明,投喂5种蛋白源不同的配合饲料,未对褐点石斑鱼4种组织中血细胞的发生情况造成显著影响。  相似文献   

8.
对斜带石斑的肝脏、脾脏、头肾和肾脏等四种组织中血细胞的发生过程进行了观察和测定。观察发现,斜带石斑的头肾、肾脏和脾脏可以产生红细胞和各种类型的白血细胞,是主要的造血器官,而肝脏只能产生白细胞,不能产生红细胞。对斜带石斑的外周血液进行涂片观察,在涂片上可区分出红细胞以及四种白细胞:淋巴细胞、血栓细胞、单核细胞和嗜中性粒细胞,它们在总的白细胞中所占比例分别为(46.56±9.53)%、(21.28±6.77)%、(17.50±8.69)%和(14.66±11.06)%。  相似文献   

9.
利用透射电镜观察九孔鲍(Haliotidaediversicoloraquatilis)的外套膜表皮细胞,结果表明细胞可分为4大类,即普通柱状表皮细胞、嗜碱性粒细胞、嗜酸性粒细胞和感觉细胞。它们在不同区域的分布、形态和数量变化与外套膜的功能分化密切相关。  相似文献   

10.
利用透射电镜观察九孔鲍的外套膜表皮细胞,结果表明细胞可分为4大类,即普通柱 表皮细胞,嗜碱性粒细胞,嗜酸性粒细胞和感觉细胞。它们在不同区域的分布,形态和数量变化与外套膜的功能分化密切相关。  相似文献   

11.
探索含CpG基序的未甲基化寡脱氧核苷酸(ODNs)对鱼类的免疫调节,将人工合成的鱼免疫刺激剂寡脱氧核苷酸序列1670(含一个CpG基序)、1670-D(含两个CpG基序)加入到离体培养的异育银鲫的头肾巨噬细胞和外周血白细胞中,采用MTT法测定其对鲤外周血白细胞增殖的影响,NBT还原法和Griess试剂显色法测定对头肾巨噬细胞的呼吸爆发的影响。结果显示,1670可显著(浓度为20.0μg·mL-1)或极显著(浓度为0.1、1.0、10.0μg·mL-1)提高异育银鲫巨噬细胞的氧呼吸爆发活性,但对氮呼吸爆发活性和外周血白细胞的增殖无显著作用;1670-D可显著(浓度为0.1μg·mL-1)或极显著(浓度为1.0、10.0、20.0μg·mL-1)增强异育银鲫巨噬细胞的氧呼吸爆发活性和外周血白细胞的增殖,且可极显著(浓度为1.0、10.0、20.0μg·mL-1)提高异育银鲫巨噬细胞的氮呼吸爆发活性。表明特定序列、特定作用剂量的CpG-DNA可增强体外培养的异育银鲫巨噬细胞和外周血白细胞的非特异性免疫应答。  相似文献   

12.
To study immune mechanism of fish lymphocyte we performed a proliferation assay and ELISA using monoclonal antibody against human IL-2. The result showed that an interleukin-2 (IL-2)-like factor was detected in the supernatant of plant haemoglutinin (PHA)-stimulated lymphocyte culture from peripheral blood, spleen and head kidney of olive flounder,Paralichthys olivaceus. The quantities of IL-2-like factor in the supernatant from different lymphoid tissues were quite different. The IL-2 like factor in the supernatant from cultured head kidney lymphocytes was much higher than those of peripheral blood lymphocytes and spleen lymphocytes (P<0.01). The IL-2 activity was found in either mouse thymocyte proliferation assay or flounder head kidney lymphocyte proliferation assay and shown to have obvious enhancing effect on proliferation of the above two types of cell. The recombinant human IL-2, (rhIL-2) was able to stimulate flounder thymocyte proliferation and used to detect the IL-2 receptor (IL-2R) on the surface of flounder lymphocyte. The cross-reaction between the lymphocytes of flounder peripheral blood and CD25(IL-2R) was detected with flow cytometry and shown that the percentage of CD25-positive cell in peripheral blood was 7.74±0.67%. This work was supported by National “973” Project G1999012003, G19999012006.  相似文献   

13.
For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was observed that the artificial injection of V. alginolyticus significantly inc...  相似文献   

14.
For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was observed that the artificial injection of V. alginolyticus significantly increased the number of erythrocytes, leucocytes, lymphocytes in peripheral blood as well as peripheral serum antibacterial activity and antibody titer of large yellow croaker, and significantly reduced the number of peripheral blood granulocytes as compared with those in the control group. No significant difference in acid phosphytase and superoxide dismutase activity of serum was detected between the two groups. It is suggested that non-specific immune factors including leucocytes and anti-bacteria substance in peripheral blood played important role at the initial stage of infection, and specific immune factors such as antibody then played important role in response to anti-infection at the latter stage. Supported by the National High Technology Research and Development Program of China (863 program, No. 2007AA09Z115) and Technology Program of Xiamen (No. 3502Z73019)  相似文献   

15.
作为一种区域文化的雷州文化,是在雷州这一特定环境中生成的,可以分为雷州得名之前和雷州得名之后两个时期。在这两大时段雷州文化研究中,人的研究是基础,即对雷州族群、雷州民系、雷州人个案的研究。  相似文献   

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