共查询到20条相似文献，搜索用时 31 毫秒
1.
以华贵栉孔扇贝Chlamys nobilis(Reeve)为材料,获得聚丙烯酰胺凝胶电泳为单一蛋白区带的酶制剂。用凝胶过滤法测得该酶的分子量为159000,由20种氨基酸组成,共1118个氨基酸残基。每个酶蛋白含有4个Zn原子。将该酶的酶制剂制成脱Zn酶后,与相同浓度过度性两价金属离子作用,其活力回升以Zn最强,Co次之。若向脱Zn酶蛋白分别加入一定量Zn原子或Co原子,根据实验结果初步推测:每个酶蛋白的4个Zn原子中有2个Zn原子先与该酶结合,与酶的催化作用有密切关系;另外2个Zn原子与酶结合,则起维持酶结构的作用,与哺乳动物和Eschericha coli来源的碱性磷酸酶研究结果一致。此外,本实验采用可见吸收光谱和圆二色谱(CD)来确证Co能很好地取代该酶蛋白内的Zn,与Simpon报道相比较,得知当Co原子加入脱Zn酶时,先加2个Co~(2 )离子结合到该酶的结构部位,而后加2个Co~(2 )离子才结合到催化部位。 相似文献
2.
The incompressible viscous uniform and shear flow past a circular cylinder is studied. The two-dimensional Navier-Stokes equations are solved by a finite element method. The governing equations are discretized by a weighted residual method in space. The stable three-step scheme is applied to the momentum equations in the time integration. The numerical model is firstly applied to the computation of the lid-driven cavity flow for its validation. The computed results agree well with the measured data and other numerical results. Then, it is used to simulate the viscous uniform and shear flow over a circular cylinder for Reynolds numbers from lO0 to lO00. The transient time interval before the vortex shedding occurs is shortened considerably by introduction of artificial perturbation. The computed Strouhal number, drag and lift coefficients agree well with the experimental data. The computation shows that the finite element model can be successfully applied to the viscous flow problem. 相似文献
3.
Within the second-order perturbation approximation, this paper investigates the physical process of generation of the time-domain second harmonic by a primary Lamb wave waveform in an elastic plate. The present work is performed based on the preconditions that the phase velocity matching is satisfied and that the transfer of energy from the primary Lamb wave to the double frequency Lamb wave is not zero. It investigates the influences of the difference between the group velocities of the primary Lamb wave and the double frequency Lamb wave, the propagation distance and the duration of the primary Lamb wave waveform on the envelope shape of the time-domain second harmonic. It finds that the maximum magnitude of the envelope of the second-harmonic waveform can grow within some propagation distance even if the condition of group velocity matching is not satisfied. Our analyses also indicate that the maximum magnitude of the envelope of the second-harmonic waveform is kept constant beyond a specific propagation distance. Furthermore, it concludes that the integration amplitude of the time-domain second-harmonic waveform always grows with propagation distance within the second-order perturbation. The present research yields new physical insight not previously available into the effect of generation of the time-domain second harmonic by propagation of a primary Lamb wave waveform. 相似文献
4.
Yoann Le Bars Florent Lyard Catherine Jeandel Leonardo Dardengo 《Ocean Modelling》2010,31(3-4):132-149
The AMANDES project aims to study transports from the Andean mountains to the Atlantic Ocean through the Amazon system. This requires realistic estuarine modelling in this area strongly forced by tides and river discharge. As none of the existing models for this region would fit the actual needs of the project, a specific new generation model has been implemented.The model is based on the hydrodynamic finite element model T-UGOm. In a first step, we limit our investigations to tidal dynamics. As the Amazon estuary is a very shallow macro-tidal area, it is necessary to improve the available bathymetries and to develop a precise bottom friction parametrisation.In this paper, we discuss the implementation of a high resolution regional model. This allows us to develop a precise and accurate tidal model: for instance, the overall root mean square error on complex differences is reduced from 54 cm in a standard model to 27 cm in our best model. Such precise and accurate tidal modelling is a prerequisite for modelling particle transport. 相似文献
5.
A numerical model is developed for estimation of local scour around a large circular cylinder under vvave action. The model includes wave diffraction around structures, bed shear stress calculation inside the vvave boundary layer and topo-graphical change model. The vvave model is based on the improved Boussinesq equations for varying depth. The vvave boundary layer is calculaled by solving the integrated momentum equation over the boundary layer. The bed shear stress due to streaming, an important factor affecting the sediment transport around a large-scale cylinder, is calculated. The Lagrangian drift velocity is included in calculation of the suspended sediment transport rates. The model is implemented by a finite element method and the results from the present model, which agree well with experimental data, are com-pared vvith those from other methods. 相似文献
6.
Prediction of streamwise flow-induced vibration of a circular cylinder in the first instability range 总被引：1，自引：0，他引：1
The streamwise flow-induced vibration of a circular cylinder with symmetric vortex shedding in the first instability range is investigated, and a wake oscillator model for the dynamic response prediction is proposed. An approach is applied to calibrate the empirical parameters in the present model; the numerical and experimental results are compared to validate the proposed model. It can be found that the present prediction model is accurate and sufficiently simple to be easily applied in practice. 相似文献
7.
基于平行双板结构的圆管尾流抑制方法研究 总被引：1，自引：0，他引：1
通过数值模拟方法对基于平行双板的圆管尾流进行抑制研究。以二维不可压缩粘性流体方程为流动控制方程,并采用稳定化流体有限元方法求解。基于数值结果,详细探讨了平行双板的安装角度和板长等关键特征参数对尾流的抑制作用和影响。数值分析发现,平行双板结构对尾流的抑制作用明显优于传统单板控制。计算结果显示,基于平行双分隔板的尾流抑制最优位置为从圆管后驻点起向上游40°~50°处,并且与单板相比,在更短板长条件下,亦可完全抑制圆管的旋涡脱落,从而尾流从非定常涡旋流转入定常流动。当安装位置为θ≥80°时,被控圆管表面压力分布与无控圆管类似,说明此时平行双板控制无减阻效应。最后,基于数值模拟结果,对本方法的尾流高效控制效应,给出了相应的流动机制。 相似文献
8.
9.
10.
In this paper, a numerical model is established for estimating the wave forces on a submerged horizontal circular cylinder. For predicting the wave motion, a set of two-dimensional Navier-Stokes equations is solved numerically with a finite element method. In order to track the moving non-linear wave surface boundary, the Navier-Stokes equations are discretized in a moving mesh system. After each computational time step, the mesh is modified according to the changed wave surface boundary. In order to stabilize the numerical procedure, a three-step finite element method is applied in the time integration. The water sloshing in a tank and wave propagation over a submerged bar are simulated for the first time to validate the present model. The computational results agree well with the analytical solution and the experimental data.Finally, the model is applied to the simulation of interaction between waves and a submerged horizontal circular cylinder.The effects of the KC number and the cylinder depth on the wave forces are studied. 相似文献
11.
海底麻坑是与流体逸散相关的一种海底凹陷地貌, 在全球海域的陆架、陆坡和深海平原等均有广泛发育。文章利用高分辨率的多波束测深数据和三维地震资料, 在中建南盆地北部识别出330个规模不等的海底麻坑, 按照麻坑形态可将其分为: 圆形、椭圆形、长条形及新月形麻坑。研究区内海底麻坑直径可达1500~7900m, 最大深度可达175m, 其中圆形麻坑规模(直径、深度)小于椭圆形、长条形和新月形麻坑, 表明圆形麻坑处于麻坑发育早期阶段。三维地震资料显示不同类型海底麻坑的下伏地层中均发育有断层、气烟囱、裂隙等流体逸散通道, 为该区域海底麻坑形成提供了有利条件。在海底麻坑演化过程中, 底流对海底麻坑的地貌形态具有改造作用。当圆形麻坑下伏地层流体活动强烈时, 流体可沿着运移通道直接向麻坑内壁渗漏, 使得其内壁塌陷, 逐渐演化成椭圆形麻坑。由于椭圆形麻坑处于底流活动影响的早期阶段, 其受底流改造作用较弱。在持续性底流活动的强烈改造作用下, 紧密排列的圆形或椭圆形麻坑逐渐拉伸演变成长条形麻坑。当底流作用于孤立的圆形麻坑时, 在底流的上游侧沉积速率增加, 麻坑在上游侧接受沉积被掩埋, 下游侧地层被侵蚀, 从而形成新月形麻坑。根据研究区海底麻坑成因机制分析, 文章首次提出了一种展示中建南盆地不同类型海底麻坑演化过程模型, 该模型有助于理解中建南盆地流体逸散过程和底流活动, 并且可为其他区域海底麻坑演化过程研究提供参考。 相似文献
12.
近壁圆柱绕流水动力特性数值模拟与实验研究 总被引：3，自引：0，他引：3
通过数值模拟和物理模型实验,对距壁面一定高度的圆柱绕流水动力特性进行了研究。数值模拟采用有限体积法对标准k-ε模式方程进行离散,采用SIMPLE算法进行求解,模拟绕流流场。在物理模型实验中,将PVC圆管制作的实验模型安放在水槽内,在圆管的跨中沿表面周向均匀布置水下压力传感器,用于测量绕流圆柱体表面动水压力分布。通过改变Re数和间隙比来分析它们对近壁圆柱绕流水动力特性的影响。基于数值流动显示技术,给出了近壁绕流流场的尾流流态分析。通过数值结果与实验结果的对比,对近壁绕流圆柱体的升力系数及其表面动水压力分布进行了研究,对比结果显示了较好的一致性。 相似文献
13.
Numerical Simulation of Local Scour Around A Large Circular Cylinder Under Wave Action 总被引：1，自引：0，他引：1
A horizontal two- dimensional numerical model is developed for estimation of sediment transport and sea bed change around a large circular cylinder under wave action. The wave model is based on an elliptic mild slope equation. The wave-induced current by the gradient of radiation stress is considered and a depth integrated shallow water equation is applied to the calculation of the current. The mass transport velocity and the bed shear stress due to streaming are considered, which are important factors affecting the sediment transport around a structure due to waves, especially in reflective areas. Wave-current interaction is taken into account in the model for computing the bed shear stress. The model is implemented by a finite element method. The results of this model are compared with those from other methods and agree well with experimental data. 相似文献
14.
为了研究斜向入射波浪,基于三维不可压缩两相流模型,开发了一套圆形数值波浪水池数值模型。在圆形波浪水池中,通过源项造波法成功生成了任意入射方向的波浪,并且利用人工摩擦项模拟阻尼区以数值耗散反射波浪。模型基于嵌入式多块网格体系,采用FVM法(finite volume method)离散Navier-Stokes方程,VOF法(volume of fluid)追踪自由水面。试验结果表明,斜向入射波浪的模拟结果与理论值基本一致,圆形波浪水池在模拟斜向入射波浪时,有效区域的面积较传统波浪水池显著增大,而且有效区域受波浪入射角度的影响也较小。同时,通过叠加多列斜向入射波浪,模拟出了多向交叉波列,并通过与理论结果对比,发现其具有较高的精度。 相似文献
15.
16.
Hydroelastic response of a circular plate in waves on a two-layer fluid of finite depth 总被引：1，自引：0，他引：1
The hydroelastic response of a circular, very large floating structure （VLFS）, idealized as a floating circular elastic thin plate, is investigated for the case of time-harmonic incident waves of the surface and interfacial wave modes, of a given wave frequency, on a two-layer fluid of finite and constant depth. In linear potential-flow theory, with the aid of angular eigenfunction expansions, the diffraction potentials can be expressed by the Bessel functions. A system of simultaneous equations is derived by matching the velocity and the pressure between the open-water and the plate-covered regions, while incorporating the edge conditions of the plate. Then the complex nested series are simplified by utilizing the orthogonality of the vertical eigenfunctions in the open-water region. Numerical computations are presentedto investigate the effects of different physical quantities, such as the thickness of the plate, Young＇s modulus, the ratios ofthe densities and of the layer depths, on the dispersion relations of the flexural-gravity waves for the two-layer fluid.Rapid convergence of the method is observed, but is slower at higher wave frequency. At high frequency, it is found that there is some energy transferred from the interfacial mode to the surface mode. 相似文献
17.
《Deep Sea Research Part I: Oceanographic Research Papers》2003,50(8):1005-1021
The main sill of the Strait of Gibraltar (Camarinal Sill) is an area of very energetic internal wave activity. The highest amplitude internal wave is the well-known internal bore, generated at critical conditions over Camarinal Sill. A very energetic lee wave has recently been found and reported. This occurs in neap tides when favorable combination of the stratification, vertical profile of horizontal background velocity, and bottom topography determines its generation. When the lee wave is developed the manifestation of high-amplitude internal waves is observed at the sea surface as high-frequency chaotic oscillations, named boiling waters. We analyze the generation of the lee wave over the main sill of Gibraltar Strait on the basis of the data from a ship mounted ADCP, multi-probe CTD data taken during a survey carried out in November 1998, and the numerical solution of the Taylor–Goldstein equation for the prevailing hydraulic conditions previous to its generation. Stratification is computed from CTD data, and the tidal current prediction is made from the 2 years of ADCP hourly data at Camarinal Sill gathered during the Gibraltar Experiment 94-96. The main characteristic is that they happen during neap tides, and their magnitude is comparable to the internal bore generated during spring tides. The classical internal bore and the lee waves are different phenomena, and the presence of the latter is an indicator of minimum flow over Camarinal Sill. A prediction model for lee waves based on the tidal hydrodynamic conditions is also developed. 相似文献
18.
19.
根据导出的海工建筑物圆弧滑动的物理数学模型,采用定积分方法对圆弧滑动的稳定性计算进行了编程实验,结果表明本方法与传统的分条法相比,具有精度高的特点,且易于在微机中实现。 相似文献