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1.
Morphology of the spermatozoon of Octopus ocellatus was studied by light, scanning electron, and transmission electron microscopes. Sperm are 600–700 μm long, with a large number of granules in diameter about 130 nm. Each spermatozoon is composed of a head, neck, and tail. The head is made up of an acrosomal complex anterior to the nucleus. The spiral acrosomal complex consists of an electron-lucent vesicle, lacuna, and an electron-dense acrosomal vesicle. Additionally, the spiral acrosomal vesicle has numerous equidistant striations, and is surrounded by many small granules (20 nm diameter). A long straight nucleus, which is electron-densed, has a deep posterior concavity, the nuclear vacuole. At the terminal end of the nucleus is a sleeve-like structure with a concave posterior nuclear fossa (PNF). The neck is short connecting the PNF. The basal body is located in the PNF and gives rise to the axoneme. This structure connects the head, neck, and tail. The tail is divided into a middle piece and a principal piece. The middle piece, having a 9+9+2 arrangement, is surrounded by a mitochondrial sheath and terminates by an electron-dense fibrous sheath. The principal piece is the longest part of the sperm with coarse fibers tapering posteriorly. The results of this study shall provide some useful information for artificial breeding of this species. 相似文献
2.
PAN Ying LI Qi YU Ruihai WANG Rucai 《中国海洋大学学报(英文版)》2008,7(1):103-107
Sperm nuclear expansion, meiosis and the association of the male and female pronuclei leading to the four-cell stage in normal Chlamysfarreri eggs were observed under a fluorescence microscope. The effects of ultraviolet （UV） irradiation on the fertilizing sperm were also examined. Both normal and UV-irradiated sperm nuclei enlarged at three distinct phases （phase A, metaphase I; phase B, polar body formation; and phase C, female pronuclear development and expansion） that were temporally correlated with meiotic process of the maternal chromosomes. Sperm nuclei underwent a rapid, initial enlargement during phase A, but condensed slightly during phase B, then re-enlarged during phase C. The effects of UV irradiation were not apparent during transformation of the sperm nucleus into a male pronucleus, and there was not any apparent effect on meiotic maturation and development of the female pronucleus. However, the rate of expansion of the UV-irradiated sperm nuclei and the size of male pronuclei were reduced apparently. Unlike the female pronucleus, the male pronucleus derived from sperm genome inactivated by UV irradiation did not form chromosomes, but became a dense chromatin body （DCB）. At mitotic anaphase, DCB did not participate in the karyokinesis of the first cleavage as evidenced by chromosomal nondisjunction, demonstrating the effectiveness of using UV irradiation to induce gynogenetic scallop embryos. 相似文献
3.
ZHANG Yanyan REN Sulian WANG Dexiu SONG Weibo 《中国海洋大学学报(英文版)》2006,5(2):132-136
Light and electron microscopic studies were carried out in order to characterize haemocytes in the bivalve mollusc Meretrix meretrix. According to nucleus and cytoplasm characters, four types of haemocytes were recognized： agranular haemocytes, lymphoid haemocyte, large granular and small granular haemocytes. Agranular hamocyte is the main cell type, accounting for 75%. It is agranular with rich organelles in cytoplasm, including mitochondria, golgi body and endoplasmic reticulum. Glycogen deposits were usually found in this cell type. The number of lymphoid haemocyte accounts for 1% - 2%. This cell type is agranular and shows a high ratio of nucleus to cytoplasm. A few organelles were found. High electrondense granules with diameters of 0.2 - 0.5μm and rich organelles were found in small granular haemocyte. The proportion of this cell type is about 15%. Rich granules of high electron-dense with diameters of 0.8- 2.4μm were found in large granular haemocyte. The proportion of this cell type is about 10%, and the quantity of organelles is the least. 相似文献
4.
The spermatozoon ultrastrucmre of left-eye flounder, Paralichthys olivaceus, was ob-served by electronic microscopy, and shown to consist of head, midpiece and tail. Some nuclear vacuoles were observed inside and along the outer part of the nucleus and double-membrane structures were ob-served between the nuclear membrane and plasma membrane. The sperm sleeve consisted of four indepen-dent parts and had more than four mitochondria. Along the sperm tail there were wing-like lateral tins. 相似文献
5.
In this study,a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase,and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules(YGI,YGII,and YGIII) that differed in electron density and acid phosphatase reaction were identified in early cleavage,morula,blastula,gastrula,trochophore,and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation,indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI(free of acid phosphatase reaction) with YGII(rich in acid phosphatase reaction) could be the way by which yolk degradation is triggered. 相似文献
6.
The gastric shield and underlying stomach epithelium of Chlymas ffarreri were examined at the light and ultrastructural levels.The results showed that the gastric shield consisted of two different size lobes joined together by a narrow middle piece,the thicker lobe was shaped like a funnel,but unclosed at the lateral side;the other lobe was irregularly triangular-shaped.The transverse section of the thicker lobe was obviously laminated and gradually decreased in thickness from the peak to the margins of the shield.The underlying epithelium bore numerous about 3μm diameter spherical processes formed by the apical plasmalemma of the epithelial cells becoming blunt pseudopodia.Microvilli and some interspersed cilia were present in the areas among the spherical processes regions where only microvilli existed.Rough endoplasmic reticulum,Golgi apparatus,different-sized electron-dense secretory granules and electron-lucent vacuoles as well las abundant mitochondria were present in the underlying epithelial cells.Fused droplets of the secretion from the underlying epithelial cells formed the gastric shield. 相似文献
7.
Formation elements logging is a neutron gamma spectroscopy logging technique used to acquire the content of elements and minerals by recording the gamma ray produced by the reaction of neutron and elemental nucleus in the formation. It has a wide usage in discriminating complex lithology and evaluating unconventional reservoir. The Monte Carlo simulation model of formation elements logging tool is established in this paper, with which the effects of tool parameters including the material and length of the scintillation crystal and source spacing on logging responses are modeled. The results show that the energy response of LaBr_3: Ce crystal is better than others, and the detection efficiency increases with the crystal length but decrease with source spacing. The above results may be used as a reference for optimizing the tool structure design. 相似文献
8.
Seasonal variability of the North Equatorial Current transport in the western Pacific Ocean 总被引：2，自引：0，他引：2
Seasonal variability of the North Equatorial Current （NEC） transport in the western Pacific Ocean is investigated with ECMWF Ocean Analysis/Reanalysis System 3 （eRA-S3）. The result shows that NEC transport （NT） across different longitudes in the research area shows a similar double-peak structure, with two maxima （in summer and winter）, and two minima （in spring and autumn）. This kind of structure can also be found in NEC geostrophic transport （NGT）, but in a different magnitude and phase. These differences are attributable to Ekman transport induced by the local meridional wind and transport caused by nonzero velocity at the reference level, which is assumed to be zero in the NGT calculation. In the present work, a linear vorticity equation governing a 1.5-layer reduced gravity model is adopted to examine the dynamics of the seasonal variability of NGT. It is found that the annual cycle of NGT is mainly controlled by Ekman pumping induced by local wind, and westward-propagating Rossby waves induced by remote wind. Further research demonstrates that the maximum in winter and minimum in spring are mostly attributed to wind east of the dateline, whilst the maximum in summer and minimum in autumn are largely attributed to that west of the dateline. 相似文献
9.
The temperature, humidity, and vertical distribution of ozone in the Antarctic atmospheric boundary layer（ABL） and their seasonal changes are analyzed, by using the high-resolution profile data obtained during the International Polar Year 2008 to 2009 at Zhongshan Station, to further the understanding of the structure and processes of the ABL. The results show that the fre- quency of the convective boundary layer in the warm season accounts for 84% of its annual occurrence frequency. The frequency of the stable boundary layer in the cold season accounts for 71% of its annual occurrence frequency. A neutral boundary layer ap- pears rarely. The average altitude of the convective boundary layer determined by the parcel method is 600 m; this is 200 to 300 m higher than that over inland Antarctica. The average altitude of the top of the boundary layer determined by the potential tempera- ture gradient and humidity gradient is 1 200 m in the warm season and 1 500 m in the cold season. The vertical structures of ozone and specific humidity in the ABL exhibit obvious seasonal changes. The specific humidity is very high with greater vertical gradi- ent in the warm season and very low with a lesser gradient in the cold season under 2 000 m. The atmospheric ozone in the ABL is consumed by photochemical processes in the warm season, which results in a slight difference in altitude. The sub-highest ozone center is located in the boundary layer, indicating that the ozone transferred from the stratosphere to the troposphere reaches the low boundary layer during October and November in Antarctica. 相似文献
10.
The Diakha gold deposit is located in the southeastern margin of the highly prospective/productive Paleoproterozoic( Birimian) Kédougou-Kéniéba Inlier. Gold mineralization is closely associated with a narrow,sub-vertical,NE-trending shear corridor. The corridor is to the east of a sinistrally reactivated D1 west-dipping fault,which emanated from SMSZ( D2 reverse-sinistral regional structure). The mineralization is hosted by fine-grained sandstone and breccias units associated with a moderate to strong hydrothermal alteration,which is dominated by albitization,carbonization,silicification,hematization,and chloritization. Diakha field data and laboratory analysis suggest the existence of two main stages of hydrothermal mineralization,namely stage I and stage II. Stage I is of a breccias type of mineralization and represents the major gold bearing stage. It isassociated with hematite + calcite + ankerite/dolomite and quartz pervasive alteration,controlled by the predominantly brittle-ductile NNE to NE-shearing structures of the main deformation event D2. Stage II is represented by the second minor hydrothermal episode,showing local quartz-tourmaline and tremolite alteration which is structurally controlled by the dipping S3 cleavage formed during D3 deformation. Petrographic and microthermometric studies of fluid inclusions from quartz veins reveal the presence of early dominant carbonic CO2-pure and aqueous-carbonic CO2-rich( LCO2+ VCO2± H2 O) in stage I. The exclusively aqueous H2 O + Na Cl ± CO2 fluid inclusions( L-type) are observed in stage II. Interpretation shows that the ore-forming fluids originated from a homogeneous H2 O-CO2 fluid during phase separation,with trapping temperatures of 250℃ to 280℃ and low salinity( 6 wt % Na Cl equiv),indicating an estimated trapping pressure for the deposit between 610 to 800 bars. 相似文献
11.
Sub-tidal barotropic current variations coupled with residual sea level fluctuation in the Bohai and Yellow Seas during wintertime are addressed in this study.The temporal evolution and spatial distribution of current fluctuation are investigated using moored acoustic Doppler current profiler data in a three-dimensional numerical model.It is found that a southward current followed by a northward current occurred in the northern Yellow Sea during the fluctuation,concurrent with a significant outflow followed by inflow through the Bohai Strait.The process is consistent from surface to bottom and is coupled with remarkable residual sea level fluctuation.This quasi three-day fluctuation with amplitude 0.2-0.3 m/s leads to 1 m/1.2 m drawdown in the northern Yellow and Bohai Seas,respectively,strongly influencing water exchange between those seas.Because this a prominent feature in the seas,it is necessary to evaluate its effect on fluctuation during winter in future studies,in particular,the northward current during the recovery phase of sea level in the Bohai and Yellow Seas regarding seasonal variation. 相似文献
12.
There are many reports that cells (protoplasts) separated from the thallus of Porphyra by enzyme can develop to normal leafy thalli in the same way as monopores. But there are few investigations on the subcellular structure of the isolated vegetative cell for ccmparison with the subcellular structure of monospores. To clarify whether the separated and cultured cells undergo the same or similar ultrastructure changes during culture and germination as monospores undergo in their formation and germination, we observed their ultrastructure, ccxnpared them with those of the monospore and found that the ultrastructure of separated and cultured cells did not have the characteristic feature as that of monospore formarion, such as production of small and large fibrous vesicles, but was acccxnpanied by vacuolation andstarch mobilization like that in monospore germination. The paper also discusses the relations between monospores and separated and cultured cells. 相似文献
13.
The sea surface height oscillation with a quasi-four-month period (SSHO4) along continental slope in the northern South China Sea (NSCS) is detected using satellite altimeter data and an ocean model simulation. The SSHO4 is at southwest of Dongsha Island, and is characterized by a wavelength of ~600 km and a southwestward phase speed of ~0.1 m/s. Crossing the climatological background SST front, geostrophic currents corresponding to the SSHO4 generally induce sea surface temperature (SST) "tongues" during January-March. The cold and warm SST tongues appear southwest of cyclonic and anticyclonic eddies, respectively. The distance between the warm and cold SST tongues is about half the wavelength of the SSHO4. The geostrophic currents play an important role in lateral mixing, as manifested by the SST tongue phenomena in the NSCS. 相似文献
14.
高抒 《中国海洋湖沼学报》2002,20(1):86-90
At a shallow water station (6 m in depth), an internal oscillation event which consisted of one or two wave-like features, with a period of 3 h and a height of 1.5 m, was observed. The velocities within the water column were modified by the event during the flood phase of the tide ; a multilayered velocity structure and intense shear were generated. Further investigations are required to understand fully the mechanism for the formation of such an event. 相似文献
15.
The excavated height of the left bank slope of the diversion power system intake in Jinchuan hydropower station is about 16o m. The stability and safety of the slope during construction and its operation/utilization become one of the most important geological engineering problems. At the same time, it is also crucial to select a safe and economic excavation gradient for the construction. We studied the problem of how to select a safe and economic slope ratio by analyzing the geological condition of the high slope, including the lithology, slope structure, structural surface and their combinations, rock weathering and unloading, hydrology, and the natural gradient. The study results showed that the use of an excavation gradient larger than the gradient observed during site investigation and the gradient recommended in standards and field practice manuals is feasible. Then, we used the finite element method and rigid limit equilibrium method to evaluate the stability of the excavation slope under natural, rainstorm and earthquake conditions. The calculated results showed that the excavated slope only has limited failure, but its stability is greatly satisfactory. The research findings can be useful in excavation and slope stabilization projects. 相似文献
16.
Surface mass balance on Glacier No. 31 in the Suntar–Khayata Range,eastern Siberia,from 1951 to 2014
Yong Zhang Hiroyuki Enomoto Tetsuo Ohata Tsutomu Kadota Tatsuo Shirakawa Nozomu Takeuchi 《山地科学学报》2017,14(3):501-512
This study presents a 64-year(1951–2014) reconstruction of the surface mass balance of Glacier No. 31, located in the Suntar-Khayata Range of the eastern Siberia, where the ablation zone is characterized by the extensive dark ice surface. We use a temperature index-based glacier mass-balance model, which computes all major components of glacier mass budget and is forced by daily air temperature and precipitation from a nearby meteorological station. The glacier shows a mean annual mass balance of –0.35 m w.e.a~(–1) during the past 64 years, with an acceleration of –0.50 m w.e. a~(–1) during the recent years. A cumulative mass loss of the glacier is ~22.3 m w.e. over the study period, about 56% of which is observed during 1991–2014. In addition to the contribution of temperature rise and precipitation decrease to recent mass loss of the glacier, an experimental analysis, in which the cleanand dark ice surfaces are respectively assumed to cover the entire ablation zone, indicates that dark ice surface, caused by insoluble impurities consisting of mineral dusts, cryoconite granules, and ice algae, plays a crucial role in the changing mass balance through enhancing melt rates in the ablation zone of the glacier. 相似文献
17.
Study on Dynamic Response of the ＂Dualistic＂ Structure Rock Slope with Seismic Wave Theory 总被引：2，自引：1，他引：1
Currently, scant attention has been paid to the theoretical analysis on dynamic response mechanism of the ＂Dualistic＂ structure roek slope. The analysis presented here provides insight into the dynamic response of the ＂Dualistie＂ structure rock slope. By investigating the principle of energy distribution, it is shown that the effect of a joint plays a significant role in slope stability analysis. A dynamic reflection and transmission model （RTM） for the ＂Dualistic＂ structure rock slope and explicit dynamic equations are established to analyze the dynamic response of a slope, based on the theory of elastic mechanics and the principle of seismic wave propagation. The theoretical simulation solutions show that the dynamic response of the ＂Dualistic＂ structure rock slope （soft-hard） model is greater than that of the ＂Dualistic＂ strueture rock slope （hard-soft） model, especially in the slope crest. The magnifying effect of rigid foundation on the dynamic response is more obvious than that of soft foundation. With the amplitude increasing, the cracks could be found in the right slope （soft-hard） crest. The crest failure is firstly observed in the right slope （soft-hard） during the experimental process. The reliability of theoretical model is also investigated by experiment analysis. The conclusions derived in this paper could also be used in future evaluations of Multi-layer rock slopes. 相似文献
18.
YANG Fengyan YANG Yongchun 《中国海洋大学学报(英文版)》2006,5(3):273-277
This paper presents a new algorithm to predict locations and severities of damage in structures by changing modal parameters. An existing algorithm of damage detection is reviewed and the new algorithm is formulated to improve the accuracy of damage locating and severity estimation by eliminating the ＂erratic assumptions and limits in the existing algorithm. The damage prediction accuracy is numerically assessed for each algorithm when applied to a two-dimensional frame structure for which pre-damage and post-damage modal parameters are available for only a few modes of vibration. The analysis results illustrate the improved accuracy of the new algorithm when compared to the existing algorithm. 相似文献
19.
This paper is concerned with the hydroelastic problem of a very large pontoon-type floating structure(VLFS) edged with a pair of submerged horizontal plates, which is a combination of perforated and non-perforated plates attached to the for-end and back-end of the VLFS. For the hydroelastic analysis, the fluid is assumed to be ideal and its motion is irrotational so that a velocity potential exists. The VLFS is modeled as an elastic plate according to the classical thin plate theory. The fluid-structure interaction problem is separated into conventional hydrodynamics and structure dynamics by using modal expansion method in the frequency-domain. It involves, firstly, the deflection of the VLFS, which is expressed by a superposition of modal functions and corresponding modal amplitudes. Then the boundary element method is used to solve the integral equations of diffraction and radiation on the body surface for the velocity potential, whereas the vibration equation is solved by the Galerkin’s method for modal amplitudes, and then the deflection is obtained by the sum of multiplying modal functions with modal amplitudes. This study examines the effects of the width and location of the non-perforated horizontal plates on the hydroelastic response of the VLFS, then the performance of perforated plates is investigated to reduce the motion near the fore-end of the VLFS. Considering the advantages and disadvantages of submerged plates without and with cylindrical holes, we propose a simple anti-motion device, which is a combination of a pair of perforated and non-perforated plates attached to the for-end and back-end of the VLFS. The effectiveness of this device in reducing the deformation and bending moment of the VLFS has been confirmed, and is compared with the results in cases without and with the submerged horizontal plates by the analysis in this paper. 相似文献
20.
The synoptic situation and mesoscale structure of an explosive extratropical cyclone over the Northwestern Pacific in March 2007 are investigated through weather station observations and data reanalysis. The cyclone is located beneath the poleward side of the exit of a 200 hPa jet, which is a strong divergent region aloft. At mid-level, the cyclone lies on the downstream side of a well-developed trough, where a strong ascending motion frequently occurs. Cross-section analyses with weather station data show that the cyclone has a warm and moist core. A ‘nose' of the cold front, which is characterized by a low-level protruding structure in the equivalent potential temperature field, forms when the cyclone moves offshore. This ‘nose' structure is hypothesized to have been caused by the heating effect of the Kuroshio Current. Two low-level jet streams are also identified on the western and eastern sides of the cold front. The western jet conveys cold and dry air at 800–900 hPa. The wind in the northern part is northeasterly, and the wind in the southern part is northwesterly. By contrast, the eastern jet carries warm and moist air into the cyclone system, ascending northward from 900 hPa to 600–700 hPa. The southern part is dominated by the southerly wind, and the wind in the northern part is southwesterly. The eastern and western jets significantly increase the air temperature and moisture contrast in the vicinity of the cold front. This increase could play an important role in improving the rapid cyclogenesis process. 相似文献