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1.
Recently evidence has been obtained for reproductive impairment in estuarine populations of Atlantic croaker exposed to seasonal hypoxia. However, it is not known whether a similar disruption of reproductive function occurs in croaker inhabiting a much larger hypoxic area, the extensive dead zone in coastal regions of the northern Gulf of Mexico extending from the Mississippi Delta to East Texas. Gonadal development in male Atlantic croaker collected in September 2008 at six sites in the dead zone was compared to that in male fish sampled from three reference sites east of the Mississippi Delta which do not experience persistent hypoxia. Croaker testes collected from the dead zone were at an earlier stage of spermatogenesis than those from the reference sites. Histological examination of the testes collected from the dead zone showed that their tubules had small lumens that contained very little sperm compared to the lumens of the reference fish. Overall, sperm production was 26.2% that of the control fish at the reference sites. This decrease in spermatogenesis at the dead zone sites was accompanied by an approximately 50% decrease in testicular growth compared to that in the reference fish. The results suggest that reproductive impairment can occur over regional scales in marine fish populations exposed to extensive seasonal hypoxia in dead zones with potential long-term impacts on population abundance. 相似文献
2.
三丁基锡(Tributyltin,TBT)能够影响鱼类生殖功能,而有关TBT对鱼类生殖细胞发育至关重要的雄激素和雌激素受体的影响还未见报道。本实验以海洋经济鱼类褐菖鲉(Sebastiscus marmoratus)为对象,研究环境水平TBT(1,10和100ng/L)对精巢雄激素和雌激素受体表达的影响。结果表明,TBT暴露能够抑制褐菖鲉精巢发育和精巢雌激素受体蛋白的水平,并且发现雌激素受体主要定位于精巢精小叶腔间隙。小叶腔间隙的雌激素受体蛋白水平降低可能影响支持细胞和间隙细胞的正常功能,从而影响精巢的正常发育。TBT暴露对雄激素受体的表达并没有显著的影响。该结果为全面了解TBT产生鱼类生殖毒性效应、途径和机制提供了新的重要的资料。 相似文献
3.
Biomarker assays are widely used as proxies for contaminant-induced effects in aquatic organisms. However, in many cases, their intrinsic natural variability due to exogenous and endogenous factors makes the interpretation of biomarker data difficult. In the present study, we investigated the natural fluctuations of six hepatic biomarkers, namely ethoxyresorufin-O-deethylase (EROD) in fish and pentoxyresorufin-O-deethylase (PROD) in crustacea, catalase (CAT), carboxylesterase (CbE), glutathione-S-transferase (GST), total glutathione peroxidase (GPX) and glutathione reductase (GR) in two deep-sea fish species, namely Alepocephalus rostratus and Lepidion lepidion and the decapod crustacean Aristeus antennatus. The NW Mediterranean deep-sea environment is characterized by very stable temperature and salinity conditions, allowing the exclusion of these two factors as potential sources of interference with biomarker activities. Biomarker results exhibited a clear influence of reproductive processes on enzyme activities, in particular in A. rostratus, which presented a pronounced seasonal pattern linked to variations in the gonadosomatic index (GSI). In addition, other factors such as food availability may also have influenced the observed variability, in particular in specimens of L. lepidion, which did not exhibit variations in reproductive activity throughout the sampling period. Depth-related variability did not exhibit a clear trend and fluctuations across sampling depths were not attributable to any specific factor. Body size had also a significant influence on some biomarkers, although allometric scaling of certain enzyme activities appears to be species-specific. The present work has thus shown that despite the lack of fluctuations of abiotic parameters such as temperature and salinity, biomarker activities in deep-sea organisms still exhibit significant variability, mainly as a result of reproductive processes and food availability. 相似文献
4.
M. Cristina Fossi Silvia Casini Stefania Ancora Alessandra Moscatelli Antonella Ausili Giuseppe Notarbartolo-di-Sciara 《Marine environmental research》2001,52(5)
Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fishes. In this research the hypothesis that Mediterranean top predator species (such as large pelagic fish) are potentially at risk due to EDCs is investigated. These marine organisms tend to accumulate high concentrations of EDCs such as polyhalogenated aromatic hydrocarbons (PHAHs). The potential effects of EDCs on a fish species of commercial interest, the top predator Xiphias gladius (swordfish), were investigated using vitellogenin (Vtg) and Zona radiata proteins (Zrp) as diagnostic and prognostic biomarkers. Dramatic induction of typically female proteins (Vtg and Zrp) was detected by ELISA and Western Blot in adult males of the species. These results are the first warning of the potential risk for reproductive function of Mediterranean top predators, and suggest the need for continuous monitoring of this fragile marine environment. 相似文献
5.
6.
Bifenthrin(BF) is a pyrethroid insecticide that is widely used in agriculture, horticulture, and for residential purposes. However, few studies addressing the reproductive toxicity of BF on fishes are available. The present study was conducted to investigate the effects of BF on testicular development in Sebastiscus marmoratus and to gain insight into its mechanism. After exposure to 1, 10 and 100 ng/L BF for 50 days, there was a reduced number of mature sperm and an abundance of the late stages of spermatocysts in the testes. The levels of 17β-estradiol and testosterone were decreased significantly after BF exposure. The activity of caspase-3 was increased in a dosedependent manner after BF exposure, TUNEL assay indicated that BF exposure resulted in the occurrence of apoptosis in the testes, which might be main reason for the inhibition of spermatogenesis. 相似文献
7.
鱼类生殖干细胞具有多向分化潜能，作为供体细胞移入同种或异种受体，在受体内迁移、定居、增殖、分化、成熟，产生供体来源功能性配子和后代，称为鱼类生殖细胞移植。作为一项辅助生殖技术，与冷冻保存技术相结合，已经在多种鱼类上进行研究。经过十几年的发展，鱼类生殖细胞移植技术逐渐成熟，目前已经建立了原始生殖细胞、精原细胞、卵原细胞作为供体细胞，囊胚胚胎、孵化幼鱼、成鱼为受体的移植体系。生殖细胞移植技术未来将在濒危物种保护、代理养殖、干细胞诱导分化等方面发挥重要作用。本文总结分析了目前已经报道的鱼类生殖干细胞移植技术，以及鱼类生殖干细胞移植存在的问题和未来发展方向。 相似文献
8.
我国鱼类生殖生理学研究概况 总被引：6，自引：1，他引：6
我国从50年代开始,根据渔业生产的需要,成功地进行了几种家鱼的人工繁殖试验。同时,也开展了鱼类生殖生理学的基础研究,使工作不断深入和提高。本文综述了我国40年来一系列有关鱼类生殖生理学的研究概况。 相似文献
9.
1 IntroductionBiogenicamine, oneofim portantbioactivesub-stancesin crustaceans,participatesin extensivephysi-ologicalactivities, suchastegum entarycolorchange,m olting,m usclecontraction,glucidem etabolism,etc.(Fingerman etal.,1994).Furthermore,ithasbeen … 相似文献
10.
M C Fossi S Casini S Ancora A Moscatelli A Ausili G Notarbartolo-di-Sciara 《Marine environmental research》2001,52(5):477-483
Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fishes. In this research the hypothesis that Mediterranean top predator species (such as large pelagic fish) are potentially at risk due to EDCs is investigated. These marine organisms tend to accumulate high concentrations of EDCs such as polyhalogenated aromatic hydrocarbons (PHAHs). The potential effects of EDCs on a fish species of commercial interest, the top predator Xiphias gladius (swordfish), were investigated using vitellogenin (Vtg) and Zona radiata proteins (Zrp) as diagnostic and prognostic biomarkers. Dramatic induction of typically female proteins (Vtg and Zrp) was detected by ELISA and Western Blot in adult males of the species. These results are the first warning of the potential risk for reproductive function of Mediterranean top predators, and suggest the need for continuous monitoring of this fragile marine environment. 相似文献
11.
Ji Yong Choi Na Na Kim Dae-Won Lee Young-Ung Choi Cheol Young Choi 《Ocean Science Journal》2017,52(3):439-446
The moon is known to be an environmental factor that controls the reproductive cycle of fish, and fish have evolved a variety of reproduction patterns depending on the lunar phase. In this study, we examined the relationship between lunar phases and the reproductive cycle of the humbug damselfish Dascyllus aruanus inhabiting Weno Island, Chuuk Lagoon, Micronesia. We divided the one-month lunar cycle into eight phases, and measured the features of moonlight (peak wavelength and intensity) and indicators of fish maturity [gonadosomatic index (GSI) and sex hormones] in relation to the eight lunar phases. In addition, we investigated the daily rhythms of sex hormones in fish ovaries during the full moon phase. The results showed that the peak wavelength of moonlight was 430 nm (blue wavelength region) regardless of the lunar phase, and that moonlight intensity was highest during a full moon at 02:00. Furthermore, the GSI and sex hormones were both higher around the full moon phase. These findings support the hypothesis that humbug damselfish spawn once a month and that this event occurs at full moon, which is the phase of the moon with the strongest intensity. Based on these findings, we predict that blue wavelength, the dominant wavelength of moonlight, is one of the environmental factors influencing the monthly spawning of D. aruanus. 相似文献
12.
M. Flain 《新西兰海洋与淡水研究杂志》2013,47(3-4):519-521
In the Marine Department archives, early records (1930–41) of marked parr of quinnat salmon Oncorhynchus tshawytscha (Walbaum) show that some held in freshwater hatchery ponds in south Canterbury survived development of testes for two consecutive years, and that one fish developed testes for three consecutive years and gave indications that it might survive into the fourth. It is generally believed that all wild adults of this species die after one spawning. 相似文献
13.
Estrogens appear to have a modulating effect on the expression of cytochrome P4501A (CYP1A) in fish. A number of in vivo studies have demonstrated that hepatic CYP1A expression in females decrease during sexual maturation when plasma levels of 17 beta-estradiol (E2) increase, or in cases when the fish in injected with E2. Since a number of environmental contaminants have weak estrogen-like activities, the question arises if these compounds are able to modulate CYP1A expression as well. In the present study, we used in vitro monolayer cultures of rainbow trout, Oncorhynchus mykiss, liver cells to compare concentration-dependent (10(-9) to 10(-5) M) effects of the natural steroid E2 and the non-steroidal xenoestrogen 4-tert-octylphenol (OP) on CYP1A-catalyzed 7-ethoxyresorufin-O-deethylase (EROD) activity. The concentration dependency of the estrogenic activity of the two test compounds was assessed by determination of hepatocellular vitellogenin (Vg) release into the culture medium. Exposure of hepatocytes to E2 concentrations of 10(-8) M and higher led to a significant inhibition of basal cellular EROD activity. On the contrary, exposure to OP did not result in an inhibition of EROD activity, even at OP concentrations (10(-6) M, 10(-5) M) which were associated with a significant induction of Vg synthesis. 相似文献
14.
Matthiessen P Allen Y Bamber S Craft J Hurst M Hutchinson T Feist S Katsiadaki I Kirby M Robinson C Scott S Thain J Thomas K 《Marine environmental research》2002,54(3-5):645-649
This paper summarises results of the EDMAR programme which is investigating oestrogenic and androgenic endocrine disruption in UK coastal waters. Most of the data concern fish. Four species (flounder, viviparous blenny and two sand gobies) are experiencing feminisation in industrialised estuaries. In males this includes vitellogenin (VTG) synthesis, ovotestis induction and/or feminised sexual characteristics. Although reproductive success may be impaired in some cases, implications for fish populations are still unclear. Suspected causative contaminants include natural oestrogenic substances and synthetic oestrogen mimics. The majority of the oestrogenic activity is adsorbed to sediments, and routes of exposure may include benthic food chain transfer. Some natural androgenic substances are also being discharged to estuaries, but their activity appears low. 相似文献
15.
Previously, we have shown that Asian clams (Potamocorbula amurensis) with highest metallic body burdens have highest prevalence of disease and lowest reproduction. The present study was designed to assess and validate potential sublethal toxicity of hexavalent chromium (Cr-VI) in clams under controlled laboratory exposure. For 7 days, three replicates of clam (n = 10 per replicate) were exposed to aqueous solution containing 0.00, 0.92, 8.40, or 25.6 mg l-1 of Cr-VI at 15 degrees C and 15 g l-1 salinity. Mortality reached 100% in the 25.6 mg l-1 group within 7 days. There was no significant difference in mortality among the control, 0.92, and 8.40 mg l-1 groups. Western blot analyses revealed significantly elevated stress protein hsp70 levels in the 8.40 mg l-1 treatment group. Histopathologic analyses revealed mild digestive gland (DG) atrophy in the control group. Clams exposed to 0.92 mg l-1 Cr-VI showed moderate DG atrophy, moderate granulomatous inflammation and necrosis in DG, ovary and testis. Lesions observed in the 8.40 mg l-1 treatment group included severe DG atrophy, severe granulomatous inflammation and necrosis in byssal gland, DG, gill, kidney, ovary and testis. In gills and testes of treated groups, apoptotic cells outnumbered mitotic cells. In addition, gills from clams in the 8.40 mg l-1 group showed enhanced hsp70 staining. Our studies support a cause-effect relationship between contaminants and reduced health in Asian clams and indicate the DGs, gills, and reproductive organs are principal targets of Cr-VI toxicity at sublethal concentrations. Results from this study suggest that Cr-VI may have played a role in the increased incidence of diseased clams seen in previous studies and these adverse effects may be working to decrease clam populations at sites with highest metallic contamination in the San Francisco Bay Estuary. 相似文献
16.
鲻鱼精子发生和形成的超微结构研究 总被引：15，自引：0，他引：15
用透射电子显微镜研究了鲻鱼精子发生和精子形成过程.结果表明,鲻鱼早期精巢有两种类型的精原细胞,即A型和B型,在B型精原细胞可见核旁有类核周体(nuage),可视为精原细胞向初级精母细胞发育分化的一个重要标志.初级精母细胞减数分裂早期同源染色体配对明显可见,精子细胞之间存在着间桥联系.鲻鱼成熟精子的特点是细胞核呈马蹄形,核凹较浅,核前端无顶体,核内染色质呈粗颗粒状,核膜与质膜之间有较大的空隙.中段较短,近端中心粒和远端中心粒互相垂直.精子尾部细长,横切面为典型的“9+2”双联微管结构,两侧有侧鳍 相似文献
17.
Boleophthalmus pectinirostris is an amphibious economic fish and wildly distributed in the southeast coast of China. In this study, Aroclor 1254 was intraperitoneally injected into B. pectinirostris with 1, 2 and 4 μg/(g·d) for 28 d to assay the reproductive organ weight, the sperm quality (sperm concentration and motility), and the testicular mitochondrial testicular mitochondria oxidative stress. The results show that the sperm number and motility in seminal vesicles, the absolute weight of testes and seminal vesicles of B. pectinirostris treated with 2 and 4 μg/(g·d) Aroclor 1254 decreased significantly as compared to the controls (p <0.05), while those treated with 1 μg/(g·d) Aroclor 1254 had no significant effects on these indictors. For the relative weight of reproductive organs, significant reduction (p <0.05) was only observed in the seminal vesicles of B. pectinirostris treated with 4 μg/(g·d). SOD activities and GSH levels in all the Aroclor 1254 treatments were significantly lower than those of the controls (p <0.05). The activities of CAT, GPx, GR and the levels of Vit C also decreased significantly in comparison with the controls (p <0.05) at the higher dose of 2 and 4 μg/(g·d) Aroclor 1254 treatments. In addition, both H2O2 level and MDA content in testicular mitochondria of B. pectinirostris had a close correlation with Aroclor 1254 dosage, and were significantly higher than the controls (p <0.05). Those indicate that Aroclor 1254 can induce the oxidative stress of testicular mitochondria, and impair the reproductive function of male B. pectinirostris. 相似文献
18.
In geographically extensive fish populations the potential exists for reproductive traits to vary over the population’s range but, the impact that such intrapopulation variability has on overall population reproductive potential has not been formally assessed. Here intrapopulation spatial variability in size at maturity and fecundity are demonstrated for Grand Bank American plaice (Hippoglossoides platessoides) and yellowtail flounder (Limanda ferruginea). Recognition of intrapopulation variability in these reproductive traits coupled with spatial variability in abundance resulted in an increase in estimated population total egg production (TEP) by as much as 1014 eggs for American plaice and 1015 eggs for yellowtail flounder as compared to assessment of TEP for the population as a whole. Results highlight the need to explore variability in life history traits not only between but, also within populations and emphasizes the need for sufficient spatial coverage during sampling in order to assess the reproductive potential of fish populations. 相似文献
19.
Oxidative damage in rainbow trout caged in a polluted river 总被引：1，自引：0，他引：1
Sewage treatment works (STWs) are a common source of chemicals entering into the aquatic environment. In order to assess effects of these effluents on oxidative stress parameters in aquatic organisms, we caged rainbow trout at five sites: upstream, near an STW effluent, and three sites downstream in the river Viskan in western Sweden for 14 days during autumn, 2006. We then measured protein carbonyls in plasma as well as 20S proteosome activity and lipid peroxidation products, i.e. MDA and 4-HNE, in liver samples. Levels of both lipid and protein oxidative damage products were elevated in fish caged near the STW effluent while 20S activity showed no differences. This argues that complex mixtures of chemicals entering into the aquatic environment do have deleterious effects on fish. Additionally, oxidative stress parameters can serve as a biomarker in aquatic organisms. 相似文献
20.
Selenium is a potent reproductive and teratogenic environmental contaminant and there are concerns over possible reproductive effects of selenium on the Sacramento splittail (Pogonichthys macrolepidotus) population, a threatened species, in California, USA. In this study, the teratogenic effects of selenium were examined in splittail embryos exposed to 0.0, 5.0, and 15.0 mg l(-1) sodium selenite for 48-h at 18.0 degrees C under static conditions, with renewal every 12 h. Embryo development was evaluated daily for abnormalities from initiation of exposure (stage 27) to initiation of exogenous feeding. At the end of evaluation, prelarvae were preserved for histological analysis. There were no significant differences in mortality or hatching success between control and exposed embryos. Exposed fish had pericardial edema and deformities of skeletal tissues (loss of tail, lordosis, scoliosis, and kyphosis). Other histological alterations were limited to dysplasia, hyperplasia and metaplasia of skeletal tissues in the deformed fish. This study showed that a short exposure of embryos during somite development has significant effects on the musculoskeletal development. 相似文献