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1.
The development of the bioaugmentation during the phytoremediation of contaminated water with diesel in pilot horizontal subsurface flow constructed wetlands was investigated for 63 days. The objective of this study was to examine the enrichment of rhizobacteria in a pilot-scale system for efficient treatment of total petroleum hydrocarbon (TPH) effluent. A consortium of three rhizobacteria strains (Bacillus aquimaris, Bacillus anthracis and Bacillus cereus), which were able to utilize hydrocarbon compounds as sole carbon sources, was injected into the constructed wetlands (batchwise operation) planted with Scirpus grossus. The TPH removals from water, without or with the addition of rhizobacteria, were found to be 72 and 84%, while from sand was found to be 59 and 77%, for each treatment, respectively. These results showed that the rhizobacteria strains could enhance S. grossus growth by decreasing diesel stress and protecting S. grossus against diesel, with 12 and 18% additional TPH removal from water and sand, respectively. Our results demonstrate that S. grossus is potential to improve the phytoremediation of hydrocarbon contaminants through inoculation with effective rhizobacterial strains.  相似文献   

2.
Aerobic fixed bed bioreactors were used to study and compare biostimulation and bioaugmentation for remediation of soil contaminated with spent motor oil. Bioaugmentation using consortium of bacteria and biostimulation using inorganic fertilizer and potassium dihydrogen orthophosphate were investigated. The bioremediation indicators used were the oil and grease content removals, total heterotrophic bacteria counts and carbon dioxide respiration rates. Results showed that biodegradations were very effective with 50, 66 and 75 % oil and grease content removal efficiencies for control, bioaugmentation and biostimulation respectively after ten weeks. Carbon dioxide respiration followed similar pattern as the oil and grease content removals. Biostimulation option has the highest carbon dioxide generation (6 249 mg/kg) and the control with the least (4 276 mg/kg). Therefore, the biostimulation option can be used to develop a realistic treatment technology for soils contaminated with spent motor oil.  相似文献   

3.
Treatment of acid mine drainage (AMD) highly rich in sulfate and multiple metal elements has been investigated in a continuous flow column experiment using organic and inorganic reactive media. Treatment substrates that composed of spent mushroom compost (SMC), limestone, activated sludge and woodchips were incorporated into bacterial sulfate reduction (BSR) treatment for AMD. SMC greatly assisted the removals of sulfate and metals and acted as essential carbon source for sulfate-reducing bacteria (SRB). Alkalinity produced by dissolution of limestone and metabolism of SRB has provided acidity neutralization capacity for AMD where pH was maintained at neutral state, thus aiding the removal of sulfate. Fe, Pb, Cu, Zn and Al were effectively removed (87–100%); however, Mn was not successfully removed despite initial Mn reduction during early phase due to interference with Fe. The first half of the treatment was an essential phase for removal of most metals where contaminants were primarily removed by the BSR in addition to carbonate dissolution function. The importance of BSR in the presence of organic materials was also supported by metal fraction analysis that primary metal accumulation occurs mainly through metal adsorption onto the organic matter, e.g., as sulfides and onto Fe/Mn oxides surfaces.  相似文献   

4.
Different bacterial and fungal strains, isolated from petroleum hydrocarbon-contaminated soil, were tested, in isolation as well as in combination, for their ability to degrade total petroleum hydrocarbon (TPH) in soil samples spiked with crude oil (2, 5 or 10 %, w/w) for 30 days. The selected combination of bacterial and fungal isolates, i.e., Pseudomonas stutzeri BP10 and Aspergillus niger PS9, exhibited the highest efficiency of TPH degradation (46.7 %) in soil spiked with 2 % crude oil under control condition. Further, when this combination was applied under natural condition in soil spiked with 2 % (w/w) crude oil along with inorganic fertilizers (NPK) and different bulking agents such as rice husk, sugarcane, vermicompost or coconut coir, the percent degradation of TPH was found to be maximum (82.3 %) due to the presence of inorganic fertilizers and rice husk as bulking agent. Further, results showed that the presence of NPK and bulking agents induced the activity of degradative enzymes, such as catalase (0.718 m mol H2O2 g?1), laccase (0.77 µmol g?1), dehydrogenase (37.5 µg g?1 h?1), catechol 1, 2 dioxygenase (276.11 µ mol g?1) and catechol 2, 3 dioxygenase (15.15 µ mol g?1) as compared to control (without bioaugmentation). It was inferred that the selected combination microbes along with biostimulants could accentuate the crude oil degradation as evident from the biostimulant-induced enhanced activity of degradative enzymes.  相似文献   

5.
An assessment was carried out on the pollution impact potentials of drilling waste generated from wells X and Y in “Eden Field,” offshore Niger Delta. Eleven samples each were collected from well X (8 treated and 3 untreated cuttings) and well Y (6 treated cuttings and 5 spent muds). The samples were subjected to three separate analyses, namely, oil-on-cutting analysis using Dean and Stark reflux method, heavy metal analysis using atomic absorption spectrophotometer (Unicam 929), and toxicity test analysis using bioassay procedure on test organisms such as Desmoscaris trispinosa and Palaemonetes africanus all in attempt to detect their pollution potential. The results showed that the oil-on-cutting analysis for the well X samples was between 15.8 and 17.5 % for unwashed cuttings and between 4.6 and 5.1 % for washed cuttings, while that for well Y samples was between 3.49 and 4.27 % for washed cuttings and 1.69 to 2.59 % for the drill mud samples. The heavy metal analysis on the wells X and Y samples showed absence of mercury, cadmium concentration ranged from 0.52 to 0.99 mg/kg for well X and from 0.69 to 0.78 mg/kg for well Y samples. The result of the toxicity test of samples from well X on D. trispinosa showed that 96-h LC50 occurred at 9800 to 10,900 mg/l for the washed drill cutting and 6200 to 6700 mg/l for the unwashed drill cuttings. On the other hand, the toxicity test on P. africanus indicated more resistant to the toxicity of the drill cuttings as 96-h LC50 was achieved at higher concentrations. At 96-h exposure, the concentration that killed 50 % of the test organism (LC50) occurred at 14,000 to 16,900 mg/l for the washed cuttings and 10,300 to 11,350 mg/l for the unwashed cuttings. Similarly, the 96-h LC50 was achieved on washed drill cuttings from well Y at concentrations of 9800 to 10,400 mg/l using D. tripinosa and 11,600 to 17,200 mg/l using P. africanus. The study has shown that oil-on-cutting content, heavy metal concentrations, and toxicity level did not indicate any major risk to the environment as the treatment and cleaning of the drilling wastes on the rig helped in reducing the concentration of all the contaminants in compliance with the regulatory limits.  相似文献   

6.
Extensive research and increasing number of potential industrial applications made ionic liquids (ILs) important materials in design of new, cleaner technologies. Together with the technological applicability, the environmental fate of these chemicals is considered and significant efforts are being made in designing strategies to mitigate their potential negative impacts. Many ILs are proven to be poorly biodegradable and relatively toxic. Bioaugmentation is known as one of the ways of enhancing the microbial capacity to degrade xenobiotics by addition of specialized strains. The aim of current work was to select microbial species that could be used for bioaugmentation in order to enhance biodegradation of ILs in the environment. We subjected activated sewage sludge to the selective pressure of 1-methyl-3-octylimidazolium chloride ([OMIM][Cl]) and isolated nine strains of bacteria which were able to prevail in these conditions. Subsequently, we utilized axenic cultures (pure cultures) of these bacteria as well as mixed consortium to degrade this IL. In addition, we performed growth inhibition tests and found that bacteria were able to grow in 2 mM, but not in 20 mM solutions of [OMIM][Cl]. The biodegradation conducted by the isolated consortium was higher than conducted by the activated sewage sludge when normalized by the cell density, which indicates that the isolated strains seem specifically suited to degrade the IL.  相似文献   

7.
This is an assessment of the degree of effects of discharged produced water in river and sediments of the Bonny estuary, Niger Delta. It involved seasonal analyses of twenty-two (22) water and thirty-four (34) sediment samples from the Bonny River. The physicochemical properties (i.e., hydrocarbon content, heavy metals, and other inorganic constituents) of the water samples were examined, while the sediments were inspected for heavy metals and organic hydrocarbon constituents that may have been sourced from the discharged produced water. In addition, the principal component and cluster analyses, index of geoaccumulation, and pollution load index plots were utilized to deduce the degree of pollution, source, and distribution of these contaminants. From the results of the analyses, the Bonny seawater is contaminated with nitrate, phosphates, nickel, ammonia, and phenols in dry season, whereas nitrate, sulfate, phosphate, total nitrogen, total petroleum hydrocarbon (TPH), and heavy metals—zinc and lead—are observed in the rainy (wet) season. Similarly, the sediments are contaminated with benzene, toluene, ethyl benzene, xylene, polycyclic aromatic hydrocarbon, TPH, phenols, and heavy metals in both seasons. Considering the outcomes of these analyses, it is evident that the composition and quality of seawater and sediments of the Bonny estuary have been strongly influenced by produced water discharges in the area.  相似文献   

8.
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9.
A wildcat may not result in a petroleum prospect; however, it may indicate potential need for frontier exploration, since petroleum could leak into economic accumulations. Carbazoles and benzocarbazoles are nitrogen compounds in petroleum and can be employed to explicitly explain migration direction and distance of leaking petroleum. The hypothesis of this study is that the mechanism of the reaction involving the attraction of carbazoles to clay minerals on the matrix of the walls along their migration pathways determines the isomer that is preferentially attached to the mineral matrix. The objectives of this study are (a) to produce a profile for carbazoles and benzocarbazoles ratios in the cap rock overlying the reservoir, (b) to compare carbazole and benzocarbazole ratios to 20S/(20S + 20R) αααC29 Sterane maturity ratios (c) to infer migration direction from the carbazoles and benzocarbazoles ratio. Samples used were side wall cores and drill cuttings of water-based mud drilling. The EOM (extractable organic matter) from various formations overlying the reservoir was obtained using Soxtherm Automatic Equipment. The TPH (total petroleum hydrocarbon) was obtained from the EOM by using a precleaned isolute C18 500 mg / 3 mL column, eluted with hexane, while the polars were eluted with dichloromethane. Fractionation into saturates and aromatics was done using a silver nitrate–silica gel column. The profiles of 1,8/1,3 and 1,8/2,4 dimethyl carbazoles and the benzocarbazoles ratios show a vertical gradient of decreasing ratio with increasing vertical distance from the reservoir. This corroborates the %VRo equivalent of 20S/(20S + 20R) αααC29 Sterane and the carbazole ratios infer vertical migration of leaked petroleum.  相似文献   

10.
Biodegradation is one of the main natural attenuation processes in groundwater contaminated with petroleum hydrocarbons. In this work, preliminary studies have been carried out by analyzing the concentrations of total petroleum hydrocarbons (TPH), dissolved inorganic carbon (DIC), dominant terminal electron accepters or donors, as well as δ 13CDIC and δ 34SSO4, to reveal the biodegradation mechanism of petroleum hydrocarbons in a contaminated site. The results show that along groundwater flow in the central line of the plume, the concentrations of electron acceptors, pH, and E h increased but TPH and DIC decreased. The δ 13CDIC values of the contaminated groundwater were in the range of ?14.02 to ?22.28 ‰PDB and ?7.71 to 8.36 ‰PDB, which reflected a significant depletion and enrichment of 13C, respectively. The increase of DIC is believed to result from the non-methanogenic and methanogenic biodegradation of petroleum hydrocarbon in groundwater. Meanwhile, from the contaminated source to the downgradient of the plume, the 34S in the contaminated groundwater became more depleted. The Rayleigh model calculation confirmed the occurrence of bacterial sulfate reduction as a biodegradation pathway of the petroleum hydrocarbon in the contaminated aquifers. It was concluded that stable isotope measurements, combined with other biogeochemical measurements, can be a useful tool to prove the occurrence of the biodegradation process and to identify the dominant terminal electron-accepting process in contaminated aquifers.  相似文献   

11.
This paper examines the influence of sorbed gas type and pressure on the strength of coal. Particular attention is focused on the influence of gas type and pressures on drillability characteristics of coal and particle size distribution of drill cuttings. Drillability of coal has a strong bearing on the strength properties of coal. Based on fracture mechanics and mechanical rock cutting, weaker rocks are easier to drill and the drill cuttings produced are generally coarse in size. Accordingly a specially designed precision drill was used to drill coal samples under both normal atmospheric and confined gas pressure conditions. Indications of changes to coal strength as a result of increased sorbed gas pressure were examined by analysing the particle size distribution of the drill flushing. A laser controlled Malvern Mastersizer S particle size analyser with measuring range between 0.05 m and 900 m was used to study the particle size distribution. This paper demonstrates that there is a definite variation in the range of particle size distribution with particles obtained from drilling coal samples not subjected to gas pressures in comparison with those obtained from samples drilled under confined gas pressure. Gas types also have an influence. Higher proportions of coarse particles were produced when the confined gas was changed from CH4 to CO2. A change in the rate of drilling under varying confining pressure and gas type was also evident. The rate of drilling in air (at normal atmospheric conditions) was slower than at higher confining pressures. The highest drill rates were obtained with CO2 confinement.  相似文献   

12.
Spent mushroom compost (SMC) is widely used as reactor matrix in passive bioreactor involving sulfate reducing bacteria (SRB) for acid mine drainage (AMD) treatment. Follow-up our previous report, recent work has been established the extent of activity, sustained organic carbon availability, and the biochemical events of successive alkalinity producing system-based chemo-bioreactor for continuous performance using SMC. Removal of iron and sulfate from influent was over 77 and 90%, respectively, for first 13 weeks, while sulfate removal efficiency suddenly dropped down to 31% thereafter. Ahead of 13th week, process failure was beginning to be noticed when available dissolved organic carbon (DOC) value dropped down to 50 mg/L. SRB population was mostly affected with DOC drought at this stage. Sulfur was one of the major elements found with other tested metals in blackish green effluent precipitate. Sulfide compounds of the tested metals were formed on both exhausted chemo-bioreactor bed and precipitate. FTIR analysis indicated that SMC was responsible for metal binding and available nutrients supply. The present study revealed the feasibility of SMC as a host for treating AMD by this chemo-bioreactor that will assist in designing the continuous treatment practice.  相似文献   

13.
Determination of total petroleum hydrocarbon distribution (TPH) in groundwater of Dezful aquifer was the main purpose of this study. The study area, which is located between latitudes 32°00′ and 32°35′?N and longitudes 48°10′ and 49°35′?E, covers about 1,920 km2 in the north of Khuzestan Province, Iran. Hydrocarbon pollutants in the area were being released into the aquifer, from a variety of sources. An oil pipe crash accident, which occurred on 19 Feb. 2009 in the vicinity of the northern part of the study area, released about 6,000 barrels of crude oil to the Karkhe River. Other possible sources of TPH in the region are asphalt factories, gas stations, and the Sabzab oil pump station. Since the main source of drinking water in the Dezful area is groundwater reservoirs, this study would be very crucial, especially when there is considerable agricultural activity in the area as well. In order to determine the presence of TPH and heavy metals in the groundwater, samples were taken from wells with different usage within two periods, i.e., in Nov. 2008 and May 2009. The second sampling operation was carried out to determine the effect of the accident in the water resources. In situ groundwater parameter measurements including pH, dissolved oxygen, temperature, and electrical conductivity were also carried out in the field. Based on the results, there are four zones in the study area which were contaminated with TPH from different origins: (1) southeast of Dezful City, which was contaminated by Shokati gas station; (2) southeast of Shush City, which was contaminated by an asphalt factory; (3) southwest of Dezful City, which was contaminated by Sabzab oil pump station; and (4) the shores of Karkhe River which were contaminated due to the pipeline crash accident. This could be a serious threat to the environment and human health because TPH concentration was higher than the EPA standard in the study area. Heavy metals were not distributed in a uniform pattern in the aquifer. The concentrations were lower than the contamination level based on the EPA drinking standard, and there was no meaningful relation between concentrations of TPH and the heavy metals. It was recommended that a monitoring network should be designed to monitor oil contaminants in the ground and surface water monthly because of importance of the water resources and presence of potential oil contaminant sources.  相似文献   

14.
Abstract In well NJ-15 of the Nesjavellir geothermal field, Iceland, the transition of discrete smectite into discrete chlorite has been studied from drill cuttings recovered at depths of less than 1714 m and over a continuous range of temperatures between 60 and 300° C. At temperatures below 180° C, the clay fractions contain mixtures of di- and trioctahedral smectites, whose layer charge increases with depth. Between 200 and 240° C, discrete smectites have transformed into smectite-rich, randomly interstratified chlorite and smecite ( R 0 C/S). Because the abundance of chlorite interlayers in this C/S is generally <20%, its presence can be detected only by electron microprobe techniques and not by X-ray diffraction. Between 245 and 265° C, both regularly ( R 1) and randomly interstratified C/S are the predominant layer silicates. Discrete chlorite first appears at approximately 270° C and coexists with minor amounts of R 0 C/S at higher temperatures.
R 0 and R 1 C/S form a nearly complete compositional series between trioctahedral saponite and discrete chlorite end-members. The interlayer cation and Si content of smectites and C/S decrease with increasing temperature. The Mg/(Mg + Fe) content of smectite, C/S, and chlorite is unrelated to temperature. The percentage of chlorite in C/S, as determined by electron microprobe analyses, increases continuously with increasing temperature, except for occurrences of smectite-rich C/S in fresh basaltic dykes which have not thermally equilibrated with the higher grade country rocks.  相似文献   

15.
Twenty-five stations were selected along the UAE coastal region to delineate the distribution and to determine the source of total petroleum hydrocarbon (TPH), total organic carbon (TOC), total Kjeldhal nitrogen (TKN), polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The concentrations of TPH ranged between 46 and 223 mg/kg and the level of TOC was in the range from 0.044 to 17.8 wt%, while TPAHs occupied the range of 5–102.2 μg/kg. On the other hand, TPCBs showed values between 60 and 205 μg/kg and TKN ranged from 104 to 1,073 mg/kg. The present study shows that the distribution of organic compounds in UAE sediments were within safe limits comparing with previous studies. The present study revealed that TOC can be used as an indicator of oil pollution in heavily oiled sediments. The highest values of TOC, TPH, TPAHs and TPCBs related to the stations covered in fine sand due to adsorption properties and the large surface areas of the grains. The evaporation of low-boiling point compounds from surface layers leads to the enrichment of sediments with a thick residual. Al Sharjah-1 exhibited the highest values of TPH and TOC at Ras Al-Khaima-5, Umm Al-Quwen-1 and Dubai-1, while the highest values of TPAHs were at KhorFakkan-9, the highest value of TPCBs was at Ajman-2, and the highest values of TKN were at KhorFakkan-1, KhorFakkan-6 and Dubai-1.  相似文献   

16.
Authorities have been applying very strict regulations for the treatment of industrial wastewater recently because of the threatening level of the environmental pollution faced. Industrial wastewater containing heavy metals is a threat to the public health because of the accumulation of the heavy metals in the aquatic life which is transferred to human bodies through the food chain. Therefore, recently, researchers have been oriented toward the practical use of adsorbents for the treatment of wastewater polluted by heavy metals. The aim of this research was to determine the retention capacity of compost for copper, zinc, nickel and chromium. For this purpose, experiments in batch-mixing reactors with initial metal concentrations ranging from 100 to 1,000 mg/l were carried. It was also observed that compost could repeatedly be used in metal sorption processes. The experiments conducted indicated that compost has high retention capacities for copper, zinc and nickel, but not for chromium. Thus, compost has been approved as a potential sorbent for copper, zinc and nickel and may find place in industrial applications. Thus, solid waste which is another source of significant environmental pollution will be reduced by being converted into a beneficial product compost.  相似文献   

17.
额济纳旗地区祥探9井揭示的石炭系—二叠系为火山岩＋碎屑岩组合,火山岩锆石U-Pb谐和年龄分布在（302.2±7.8）-（319.9±4.5）Ma之间,对应的地层时代相当于晚石炭世,岩性组合特征与大狐狸山剖面干泉组（C2P1g）可以对比,确定为干泉组。该井揭示石炭系—二叠系1027.4m（未穿）,其中4m和4m以上的暗色泥岩（或粉砂质泥岩）20层,累计厚度288.5m,占地层厚度的28.1%。少量钻井岩心样品进行的烃源岩地球化学特征分析表明,有机质丰度中等—好,干酪根类型为Ⅰ—Ⅱ1型,有机质演化进入成熟—高成熟阶段,显示了良好的生烃条件。与邻近的大狐狸山剖面干泉组烃源岩样品分析结果对比,祥探9井取得的样品有机质丰度显著高于地表剖面样品,TOC为地表剖面样品的1.8倍,热解产油潜量（S1＋S2）为地表剖面样品的295.3倍,氯仿沥青＂A＂为地表剖面样品的32.8倍,总烃（HC）为地表剖面样品的42倍。干酪根类型评价指标分析,井下岩心样品H/C-O/C能较好地反映干酪根类型,地表剖面样品受风化作用的影响H元素显著降低,H/C-O/C无法进行干酪根类型评价,说明风化作用对烃源岩丰度和类型评价指标的影响较大。  相似文献   

18.
Metamorphosed black shale formations 120–150 m thick with median concentrations of 7% non-carbonate carbon and 6–8% sulphur are abundantly met in drill cores in the Kainuu and Outokumpu areas. Carbon isotope 3C values are comparable to the average isotopic composition of non-carbonate carbon in sedimentary rocks. The rare earth element patterns show cerium depletion, indicating a marine origin. Base metal concentrations in black shales are high in the Talvivaara mineralization (ore estimate: 300 Mt with 0.26% Ni, 0.14% Cu and 0.53% Zn) and in the vicinity of ophiolite complexes. In the Kainuu schist belt, the median value for gold is 35 ppb (max 170 ppb) in the western part and 16 ppb (max 180 ppb) in the east. Highest platinum and palladium concentrations encountered are 60–70 ppb. It is shown that in the Kainuu black shales, concentrations of sulphur, base metals and precious metals have been increased by hydrothermal processes, as indicated by positive europium anomalies, elevated mercury concentrations (max 7.5 ppm), high sulphur isotope 34S values and the enrichment of sulphur and base metals relative to carbon. The processes operative during the deposition of these shales find an analogy in recent processes in ocean ridge spreading axes.  相似文献   

19.
This method pertains to oil and gas geology and to geology of sedimentary basins prospective for oil and gas. It includes identifying catagenesis zones in drilled areas within the sedimentary cover of the basin based on assay results for drill cores and cuttings using the standard methods. Analysis is primarily made in order to determine rock catagenesis based on the optical characteristics of vitrinite. A correlation between catagenesis zones and layer seismic velocities obtained from regional and exploration seismic data is made for a drilled area. Both the layer seismic velocities and the degree of rock catagenesis increase with depth under the influence of increasing rock density. Correlations between layer velocities and the degree of rock catagenesis have been established. The following ratios have been determined for the Scotian shelf, Canada, and the Barents shelf, Russia: a protocatagenesis zone (the cap) corresponds to layer seismic velocities (V lay) of 1.5–3.3 km/s, a mesocatagenesis zone (the principal hydrocarbon generation area) corresponds to V lay of 3.3–5.0 km/s, and an apocatagenesis zone (an area with a very low hydrocarbon potential) corresponds to V lay of over 5.0 km/s. An advantage of the new method of identification of catagenesis zones is that it can be used prior to drilling. Its conceptual originality and cost efficiency lie precisely in this.  相似文献   

20.
Spent mushroom compost (SMC) is commonly used as a carbon source for passive treatment systems in South Korea; however, it has some drawbacks, such as sulfate release from itself. Consequently, investigations to identify effective substitutes for SMC are necessary. In this study, batch experiments were conducted for 27 days to evaluate the efficiency of rice wine waste (RWW) for reducing sulfate and removing dissolved metals within synthetic acid mine drainage (AMD). The results showed that RWW could be more suitable than SMC, which even released sulfate in the early stage of the experiment, for sulfate reduction by sulfate-reducing bacteria. Both materials produced similar results with respect to the removal of dissolved metals, such as Fe and Al. Furthermore, a mixture of SMC and RWW showed the greatest efficiency in sulfate removal. Overall, both RWW and the mixed carbon source showed comparable performance to SMC, which indicated that RWW had a great potential for use as a carbon source for AMD treatment.  相似文献   

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