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一边是蒜苗、菠菜、生菜等各种时令蔬菜,一边的猪圈内有五头将要出栏的肥猪.猪圈旁边是沼气池,沼气池为水泥结构,圆锥形,上边留一个直径有半米的圆孔以便向内填充植物垃圾和秸秆,其余的地方用土填好以后还可以种上菜,墙根没有硬化的地方,还零落地留着苦瓜、南瓜的枯藤.这是日前笔者在修武县城关镇河北辛庄村民范庆河家中看到的景象. 相似文献
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学文 《资源导刊(河南)》2007,(9)
这是一个令人振奋的喜讯. 据河南省国土资源厅于2007年9月5日召开的新闻发布会透露,截至8月底,全省已有4个省辖市、53个县(市、区),在经历了一年多的艰苦、细致工作后,终于关闭了辖区内的所有砖瓦窑厂,与"秦砖汉瓦"彻底告别.让我们记住吧,记住这4个省辖市的名字.它们分别是:漯河市、安阳市、鹤壁市、焦作市.让我们记住吧,记住这53个县(市、区)的名字.它们中有:舞阳县、临颍县、召陵区、文峰区、淇滨区、修武县、孟州市、伊川县等. 相似文献
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修成国 《资源导刊(河南)》2007,(3)
法国有许多在世界上驰名的标志性建筑或胜地,仅巴黎就有卢浮宫、凡尔赛宫、凯旋门、埃菲尔铁塔、巴黎圣母院等.笔者这里要说的是那条尽人皆知的香榭丽舍大街. 一些历史性的建筑往往标志着一个城市或地区的文化含量.而一条大街则常常标志着这一个城市或地区经济上的繁荣.如美国纽约的华尔街金融大街;日本的银座和日本桥商业街;我国北京的王府井商业大街,都成为这个城市甚至国家的代表.香榭丽舍大街则是巴黎乃至法国的代表. 相似文献
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浙东大地,河山锦绣,景奇色美.其中许多景区藏而不露,少为世人所知,东海之滨的桃渚便是一例.
桃渚景区是8000万年前火山喷发而形成的独特的火山遗迹地貌,即喀斯特地貌.山上古洞幽壑遍布,奇岩秀石突兀,断崖索桥点缀,处处皆是大自然的神来之笔,令人叹为观止.与天台、雁荡等名山大川相比,亦毫不逊色.怪不得当年文天祥逃出元军的囚禁,落荒从海道经桃渚时,被桃渚雄奇瑰丽的风景所感动,情不自禁赋诗赞美:"海上仙子国,邂逅寄孤蓬.万象图画里,千崖玉界中." 相似文献
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于文 《资源导刊(河南)》2007,(3)
亲爱的读者朋友,正所谓"春风送佳音,百花迎宾来".当您在办公室里、在田间地头、在行进的火车上捧起本期杂志时,您可能会有一个惊奇的发现:几年来,您一直热情关注和钟爱的《河南国土资源》杂志,经国家新闻出版署批准,已正式更名为《资源导刊》了.我国期刊园林又盛开了一朵鲜艳的花朵. 相似文献
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嵌入式技术以及信息技术的发展使得越来越多的设备接入网络成为可能,这两项技术的融和具有广泛的应用前景.首先分析了嵌入式系统网络化的需求,结合对嵌入式操作系统Vxworks和对ARM芯片的研究,分析了相关网络协议,最后提出构建嵌入式WebServer的软硬件解决方案. 相似文献
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A field monitoring system was established in an active river bank landslide in the Three Gorges area, China, and a consecutive monitoring for about 5 years were conducted to understand the displacement characteristics of flexible piles and the surrounding soil. It was found that piles deformed elastically under reservoir operation, and the soil in front of piles was gradually separated from piles. The movement of the pile heads exceeded that of the soil between and behind piles. This phenomenon was further studied by a large-scale physical model test to gain insights into the pile-soil interaction. The displacement relationship between pile heads and the surrounding soil is in good agreement with the field data. The physical model test shows that the deformation process of pile-reinforced landslides can be divided into two stages: firstly, when the piles head movement exceeds soil movement, the soil arching is mainly affected by the deflection of the piles, the arches between and behind piles bent upwards;but when the soil movement exceeds piles head movement, the arches near the upslope and downslope bent downwards and upwards, respectively. Furthermore, the different deformation of two adjacent piles and the pile stiffness influenced the arch’s shape and formation;the flexible piles exhibit great coordinated deformation with the landslide, and caused the soil arch on the downslope. 相似文献
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在嵌入式产品开发中,除操作系统之外,开发人员关注最多的一个系统软件组件就是图形支持系统.只要是面向人机交互的嵌入式产品,就涉及到文字或者图形的输出.通过比较各种嵌入式Linux平台上的GUI系统的结构和特点,最终选定了MiniGUI作为研究对象.重点研究并实现了MiniGUI在嵌入式Linux上的移植以及Thttpd的GUI控制界面的实现方法及过程,最终在MagicARM2410开发板上成功实现了Thttpd的GUI控制界面.通过GUI界面能够实现对Thttpd的启动、停止、重新启动的功能,实现了GUI控制界面的用户登录限制而且允许用户在登录后更改用户密码. 相似文献
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用RT-PCR和RACE方法,从荷那龙罗非鱼(Oreochromis hornorum)垂体中克隆到生长激素促分泌素受体(GHSR)cDNA全序列。荷那龙罗非鱼GHSR基因具有GHSR-1a与GHSR-1b两个高度保守cDNA序列。GHSR-1a序列全长1 646 bp,包括225 bp的5′非编码区,266 bp的3′非编码区和1 155 bp的开放阅读框,编码384个氨基酸残基,具有7个跨膜结构域结构(transmembrane domains,TM);GHSR-1b序列全长1 877 bp,包括225 bp的5′非编码区,755 bp的3′非编码区和897 bp的开放阅读框,编码298个氨基酸残基,只具有前5个TM,在第6个TM的第4个氨基酸处开始缺失。将GHSR cDNA序列与基因组序列比较发现,这两种cDNA转录本来自同一个基因的不同变体。 相似文献
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Shuo Li Chunmei Li Xubo Wang Yanan Wang Zhipeng Liu Teng Zhai Quanqi Zhang 《中国海洋大学学报(英文版)》2010,9(1):59-64
A novel immune-related gene was expressed in Japanese flounder (Paralichthys olivaceus) injected with Vibrio anguillarum. The complete cDNA contained a 169 bp 5’UTR, a 336 bp open reading frame (ORF) encoding 111 amino acids and a 556bp 3’UTR.
Six exons and five introns were identified in the PoIR2 gene. Blastp similarity comparison showed its encoding protein had 50% similarity to Danio rerio neuromedin S (NMS), but further alignment indicated they did not have NMS C-terminal conservational signature domain. So
it was not defined as an NMS homologue. Protein structure analysis indicated it had a 26aa signal peptide and was a secretory
pathway protein. RT-PCR demonstrated that the expression of PoIR2 was quickly induced and drastically increased in liver, kidney, spleen, gills, intestine, heart, and skeletal muscle after
infected with V. anguillarum. These results indicated that the PoIR2 might play some important role in Japanese flounder immune response system. This gene was named PoIR2 (P.olivaceus immune-related gene 2, GenBank accession number: EU224372). The mature PoIR2 peptide was expressed in BL21(DE3) pLysS using pET-32a(+) vector and a great part of the recombinant mature peptide existed
as soluble type. 相似文献
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Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain... 相似文献
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《中国海洋大学学报(英文版)》2015,(6)
The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B(Bf) serves as the catalytic subunit of complement component 3(C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber(Apostichopus japonicus), termed Aj Bf, was obtained by rapid amplification of c DNA ends(RACE). The full-length c DNA of Aj Bf was 3231 bp in length barring the poly(A) tail. It contained an open reading frame(ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR(5'-terminal untranslated region) and a 384 bp 3'-UTR. Aj Bf was a mosaic protein with six CCP(complement control protein) domains, a VWA(von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of Aj Bf protein was 101 k Da. Quantitative real time PCR(q RT-PCR) analysis indicated that the expression level of Aj Bf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that Aj Bf expressed higher in coelomocytes than in other four tissues. In addation, Aj Bf expression in different tissues was induced significantly after LPS or Poly I:C challenge. These results indicated that Aj Bf plays an important role in immune responses to pathogen infection. 相似文献
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以勒氏笛鲷(Lutjanus russellii)基因组DNA为模板,采用同源克隆的方法,获得2 887 bp的肌肉生长抑制素(myostatin,MSTN)基因组序列,该MSTN序列具有3个外显子和2个内含子,包括101 bp的5′-UTR、385bp的外显子1、354 bp的内含子1、370 bp的外显子2、761 bp的内含子2、381 bp的外显子3和1 932 bp的3′-UTR。整个开放阅读框编码了378个氨基酸,前面的22个氨基酸为信号肽,具有9个保守的半胱氨基酸及一个RVRR的蛋白酶解加工位点。氨基酸序列分析发现,该基因编码的蛋白质与其他鱼类的I型同源性较高,与其他鱼类的2型MSTN同源性较低,且与鲈形目的同源性最高,与鲤形目的同源性较低,与人、鼠和鸡的同源性最低。采用邻接法(Neighbor-Joining)构建的MSTN的系统发育树表明,勒氏笛鲷MSTN与鲈形目鱼类的狼鲈属亲缘关系较近,且与鱼类的MSTN-1聚为1支。这表明该基因属于Ⅰ型MSTN基因。 相似文献
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大口黑鲈抗菌肽hepcidin cDNA序列和结构分析 总被引：2，自引：0，他引：2
以大口黑鲈为材料,提取肝脏总RNA,经RT-PCR扩增出hepcidin cDNA的开放阅读框(ORF)及3′端非编码区序列,应用5′RACE方法得到大口黑鲈hepcidin cDNA5′末端。将所获得的两个片段分别克隆到T载体后进行测序,并拼接成大口黑鲈hepcidin全长cDNA。序列分析表明:大口黑鲈hepcidin全长cDNA为564bp,含有一个258bp的ORF,编码86个氨基酸残基,由信号肽(24个残基)、前肽(42个残基)和成熟肽(20个残基)3部分组成hepcidin前体。在前肽部分具有前肽转化酶典型的RX(K/R)R基元,成熟肽部分含有8个保守的半胱氨酸残基,可形成四个链内二硫桥,使β-折叠结构保持稳定。大口黑鲈Hepcidin与其他鱼类的同源性在29.7%~90.5%间,尤其是信号肽区域,与鳜、尼罗罗非鱼、真鲷、花鲈、黑鯛、金眼狼鲈仅有2~3个氨基酸的差别。 相似文献