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1.
The morphology and infraciliature of a marine ciliate Anphi/eptus litonotiformis nov. sp. inhabiting a shrimp farming pond in Sheyang (Jiangsu Province) are described. According to the infraciliature, habitat . and other morphological characters, it can not be congeneric with any other species of the genus . The special features of A. litonotiformis are the Litonotus-like body shape and the arrangement of its extrusomes regularly located along the margin of the cytostome. The most important characters of some related Amphileptus-species from marine water are biometrically tabled and discussed.  相似文献   

2.
The characteristics and infraciliature of a new euplotid ciliate,Euplotes qingdaoensis n.sp.,discovered from the coastal waters of Jiaozhou Bay,northern China,were studied using live observation and silver impregnation.The new marine species is characterized by a slightly fl attened body(90–110 μm×70–80 μm,in vivo),an adoral zone of 26–29 membranelles,10 strong frontoventral and fi ve transverse cirri,two caudal cirri,a single left marginal cirrus,seven dorsal kineties,a middle kinety with about 15 pairs of basal bodies,a silverline system of the double-eurystomus type,and a C-shaped macronucleus.  相似文献   

3.
The living morphology and infraciliature of a marine ciliate, Cardiostomatella vermiformis isolated from a sand beach of the Jiaozhou Bay near Qingdao, China, were investigated by live observation, protargol and silver carbonate impregnation methods. Both the morphological and morphometric data largely agree with former records. Based on the data obtained, an improved diagnosis for Cardiostomatella vermiformis is supplied: Large marine Cardiostomatella with cylindrical body shape and several prolonged caudal cilia; cells in vivo about (90–500) μm×(30–120) μm; macronucleus beaded; single contractile vacuole caudally positioned; buccal apparatus conspicuously small, genus typical; three to six postoral kineties; 96-130 somatic kineties. This species is recorded for the first time in China.  相似文献   

4.
As one of the main groups of marine planktonic protozoa, oligotrich ciliates have shown a high biodiversity. Recently, a new aloricate oligotrich ciliate, Strombidium pseudorapulum sp. n. was isolated from coastal waters of southeast China. The living morphology and infraciliature of this species were studied using live cell observation and protargol impregnation. Strombidium pseudorapulum sp. n. differs from its congeners by having a radish-like body with a bumped anterior end and an elongated tail-like posterior end, possessing a red ‘eyespot' in apical protrusion of cell, adoral zone of membranelles composed of 12–16 collar and 9–12 buccal membranelles, one spindle-shaped macronucleus, girdle kinety in anterior 2/5 of cell with 45–60 densely spaced dikinetids, and ventral kinety occupying about the posterior 1/3 of the cell with 16–19 dikinetids. Strombidium pseudorapulum sp. n. clusters together with those species having ‘eyespot' in apical protrusion in the phylogenetic tree based on the small subunit rRNA gene sequence. It is a sister species of S. cuneiforme with 31 unmatched nucleotides between their SSU rRNA gene sequences.  相似文献   

5.
One new marine oligotrich ciliate, Omegastrombidium hongkongense n. sp., was isolated from a bloom of Noctiluca scuntillans near Port Shelter, Hong Kong. The morphology and infraciliature of this new species were studied on both living and protargol-stained specimens. Its phylogenetic position was discussed based on the sequence of the small subunit r RNA gene. O. hongkongense is different from its congeners with special characters. The cells are usually heart-shaped, and the cell size usually is(20–35) ×(20–30) μm in vivo. Its deep buccal cavity extends obliquely to about 1/2 of cell length. It shows prominent apical protrusion. The adoral zone of membranelles is divided into 17–19 collar membranelles and four buccal membranelles. It has one ball-like macronucleus. The girdle kinety forms a closed loop which obliquely surrounds the body. The ventral kinety and thigmotactic membranelles are not observed. The SSU r RNA sequence of O. hongkongense was close to those of Strombidium paracalkinsi and Varistrombidium kelum with approximately 99% similarity. In the phylogenetic trees, O. hongkongense can be grouped with O. elegans and V. kielum species with very low support(16% ML).  相似文献   

6.
Zhang  Yu  Fu  Xiaoting  Duan  Delin  Xu  Jiachao  Gao  Xin 《中国海洋湖沼学报》2019,37(3):815-824
Agar, agarose, and agaropectin were extracted from the red alga Ahnfeltia plicata, and their properties and structures were characterized. Agar was extracted by a comparatively low alkaline consumption of 1.2%. It exhibited a gel strength of 1 152.50±74.25 g/cm~2 and a sulfate content of 0.55%±0.08%. The yield of agar from A. plicata was 24.53%, which is higher than those of other agarophytes commonly used in China. Three kinds of the method were compared for the purification of agarose, and the physicochemical properties of agarose that was prepared under the optimal condition were identical to those of commercially available agarose. Furthermore, agaropectin was purified from A. plicata and characterized by GC, HPLC,UV-spectrum, and FI-IR to understand its composition and structure. It was the first time to comprehensively study the agar and its fractions from the red alga of A. plicata. This research provided an eco-friendly agar extraction method from A. plicata and revealed its potential application for the production of agar, agarose,and agaropectin.  相似文献   

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8.
The morphology and infraciliature of a new marine colepid ciliate, Apocoleps magnus gen. nov., spec. nov., are described based on living observations and silver impregnations. The new genus Apocoleps is characterized by having 8 (vs. 6 in most other related genera) armour tiers, spines at both ends of the cell, 3 adoral organelles and plates with 4 reniform uni-windows. Apocoleps magnus spec. nov. is defined by the following features: body elongated and slightly curved, about 100–120μm× 35–45 μm in vivo; an...  相似文献   

9.
The wild Porphyra yezoensis collected from the Qingdao coast was used to prepare protoplasts by enzyme digestion. The pure line was constructed by cultivating the protoplasts. The 18S rDNA of the P. yezoensis pure line was cloned and sequenced. Sequence analysis was executed for this sequence and other 22 sequences retrieved from GenBank. A phylogenetic tree was constructed using the neighbor-joining method. The results revealed a high diversity of 18S rDNA sequences in genus Porphyra and the considerable variation of 18S rDNA sequences in different strains of the same species P. yezoensis and P. tenera. Significant difference of 18S rDNA sequence was observed between P. yezoensis from Qingdao, China, and the two strains of P. yezoensis from Japan, but the three strains of P. yezoensis formed a stable clade in the phylogenetic tree. These results indicate the possibility of interspecies and intraspecies discrimination of Porphyra using the 18S rDNA sequences.  相似文献   

10.
The Changjiang River estuary and adjacent waters are one of the most notable regions for red tides/harmful algal blooms in China's coastal waters.In this study,phytoplankton samples were collected and analyzed during the outbreak stage of red tides in May 2009.It was found that dinoflagellates,Prorocentrum donghaiense and Karenia mikimotoi,and diatoms,Skeletonema spp.and Paralia sulcata,were the major taxa dominating the phytoplankton community.Cluster analysis,non-metric multidimensional scaling(NMDS) and analysis of similarities(ANOSIM) was conducted on a data matrix including taxa composition and cell abundance of the phytoplankton samples.The analyses categorized the samples into three groups at a similarity level of 30%.Group Ⅰ was characterized by estuarine diatoms and distributed mainly in the highly turbid estuarine region.Group Ⅱ,which was dominated by the diatom Skeletonema spp.and represented the red tide of Skeletonema spp.,was situated around Group Ⅰ in the sea area west of 122°50'E.Group Ⅲ was characterized by a high proportion of dinoflagellates and was found further offshore compared with Groups Ⅰ and Ⅱ.Group Ⅲ was further divided into two subgroups(Ⅲ-S1 and Ⅲ-S2) at a similarity level of 40%.Group Ⅲ-S1 was characterized by the presence of the benthic diatom P.sulcata,representing phytoplankton samples collected either from the bottom or from the sea area affected by upwelling.GroupⅢ-S2 was dominated by dinoflagellates and represented red tides formed by P.donghaiense and K.mikimotoi.A gradual change of red-tide causative species was observed from the estuary to the offshore sea area,from diatoms to armored dinoflagellates and then unarmored dinoflagellates.Environmental factors associated with each group,and thus affecting the distribution of phytoplankton and red tides,are discussed.  相似文献   

11.
The morphology and infraciliature of a marine ciliateAnphileptus litonotiformis nov. sp. inhabiting a shrimp farming pond in Sheyang (Jiangsu Province) are described. According to the infraciliature, habitat, and other morphological characters, it can not be congeneric with any other species of the genus. The special features ofA. litonotiformis are theLitonotus-like body shape and the arrangement of its extrusomes regularly located along the margin of the cytostome. The most important characters of some relatedAnphileptus-species from marine water are biometrically tabled and discussed.  相似文献   

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13.
The living morphology, nuclear apparatus, infraciliature and silverline system of the poorly-known ciliatePseudocohnilembus hargisi Evans & Thompson, 1964, collected from a shrimp culture pond in Qingdao, China, were examined by observation of the live specimen; and by the pyridinated carbonate method and Chatton-Lwoff technique. The main characteristics: 13–14 (usually 13) longitudinal or slightly spirally arranged somatic kineties; single contractile vacuole with usually 2 (1–2) pores at posterior ends of the fourth and fifth somatic kineties to the right of the buccal cavity. Several related congeners were compared and morphometrically tabulated. Project No. 39970098 supported by NSFC.  相似文献   

14.
The living morphology and infraciliature of two rare marine ciliates, Placus salinus Dietz, 1964 and Strombidium apolatum Wilbert and Song, 2005, collected from the coastal waters near Qingdao, China, were investigated by in vivo observation and protargol impregnation technique. The improved diagnosis for Placus salinus is as follows: medium-sized marine Placus, in vivo (50–60)μm×(30–40)μm; cell elliptical to barrel-shaped; 28–31 somatic kineties; single macronucleus usually ellipsoid and one micronucleus located in the indention of the macronucleus; one contractile vacuole posteriorly positioned. Strombidium apolatum is characterized by: marine strombidium (40–60)μm×(30–45)μmm in vivo, cordiform in shape with somewhat pointed posterior end and conspicuous apical protrusion; extrusomes prominent, about 15μm in length and evenly arranged along the circle kinety; about 16 collar and 5–6 buccal membranelles; one elongate macronucleus and one micronucleus; circle and ventral kineties consisting of about 53 and 45 dikinetids respectively.  相似文献   

15.
INTRODUCTIONScuticociliateshavelongbeenknowntobefoundingreatabundanceinallkindsofhabitats,andhavegreatdiversityinspeciesnumber,structure ,behaviorandotherbiologicalcharacters.Asacommonly foundtaxonfromcoastalwaterandculturebiotopes,Pseudocohnilembusharg…  相似文献   

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The living morphology and infraciliature of two rare marine ciliates, Placus salinus Dietz, 1964 and Strombidium apolatum Wilbert and Song, 2005, collected from the coastal waters near Qingdao, China, were investigated by in vivo observation and protargol impregnation technique. The improved diagnosis for Placus salinus is as follows: medium-sized marine Placus, in vivo (50-60)μm× (30-40)μm; cell elliptical to barrel-shaped; 28-31 somatic kineties; single macronucleus usually ellipsoid and one micronucleus located in the indention of the macronucleus; one contractile vacuole posteriorly positioned. Strombidium apolatum is characterized by: marine Strombidium (40-60) μm× (30-45)μm in vivo, cordiform in shape with somewhat pointed posterior end and conspicuous apical protrusion; extrusomes prominent, about 15μm in length and evenly arranged along the circle kinety; about 16 collar and 5-6 buccal membranelles; one elongate macronucleus and one micronucleus; circle and ventral kineties consisting of about 53 and 45 dikinetids respectively.  相似文献   

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