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1.
The freshwater catfish, Mystus vittatus, were exposed to the LC50, 96 h dose (i.e. 550 ppm.) of the agro-fertilizer NPK for 96 h, during the spawning period (March–June). Their gonads were biochemically analysed for the total protein content and the free amino acids. The protein content of the gonads of the stressed fish was found lowered. The fish also showed disappearence of certain amino acids from their gonads.  相似文献   

2.
Specimens of either sex of the freshwater catfish Mystus vittatus were exposed to an acute toxic dose of an agro-chemical NPK, i.e. 550 ppm during the spawning phase of their annual reproductive cycle. The effects on total lipid and its various fractions, viz. phospholipids and cholesterol, were studied in the testes and ovary. The total lipid content of the gonads of unstressed fish showed lowest values during the spawning phase when compared to other phases, the content remained higher in the ovary than in the testes. In fish subjected to NPK-stress during the spawning period, the fall in total lipid content of the gonads was found to be much greater than that in the controls. The phospholipids content of the gonads of unstressed fish showed highest values during the spawning phase when compared to other phases; the content remained higher in the ovary than in the testes. In fish subjected to NPK-stress during the spawning phase the rise in the gonads was found to be higher than that in the control. The cholesterol content of the gonads of unstressed fish showed highest values during the spawning phase when compared to other phases; the content remained higher in the ovary than in the testes. In fish subjected to NPK-stress during the spawning period the rise in the cholesterol content of the gonads was not found to be as high as that in the controls. Agro-chemical NPK seemed to effect the total lipid and cholesterol content adversely during the spawning phase, affecting the vitellogenic process without affecting the biosynthesis of phospholipids as such, which on the contrary increases. The decrease of lipid and cholesterol mobilization from liver to gonads seemed to be restricted as a result of NPK action.  相似文献   

3.
溯河洄游长江刀鲚(Coilia nasus)摄食虾类的调查   总被引：4，自引：0，他引：4  
姜涛  刘洪波  李孟孟  杨健 《湖泊科学》2018,30(2):458-463
刀鲚(Coilia nasus)是长江的名贵经济鱼类.虽然传统上认为其溯河生殖洄游全过程不会摄食,但该问题一直尚未完全弄清.作为有效解明这一问题的第一步,本研究在前期长江流域干流和湖泊刀鲚资源调查的基础上,先利用耳石微化学技术筛选出长江河口区、江苏江段、安徽江段和鄱阳湖水域溯河洄游型的刀鲚个体,再对其胃、肠容物的大型游泳动物(鱼、虾类)进行调查分析.结果发现,在河口区、江苏和安徽江段所有刀鲚个体胃充塞度为0级,均未发现摄食有游泳动物;而鄱阳湖水域刀鲚个体的胃充塞度达4~5级,均发现有摄食淡水虾类的日本沼虾(Macrobrachium nipponense)和秀丽白虾(Exopalaemon modestus)的情况.其中29%和71%的刀鲚胃中分别含有1只和2只虾.所有刀鲚个体肠内均未发现有内容物.鉴于鄱阳湖已被确定为溯河洄游型刀鲚的产卵场之一,结果表明长江刀鲚在经河口,通过长江江苏和安徽江段干流到达鄱阳湖产卵场的过程中应该不会摄食大型游泳动物;而进入鄱阳湖产卵场后会开始摄食日本沼虾和秀丽白虾.这种两阶段的现象可能反映出了刀鲚的一种在洄游通道上节约能量,以利长距离溯河;而在产卵场补充能量,以利于性腺最终成熟的生存策略.  相似文献   

4.
三峡水库调度运行改变了库区江段的水文情势,影响漂流性卵苗在库区江段内的时空分布特征。在水库低水位运行时,具有流水生境特征的库中和库尾江段是产漂流性卵鱼类在整个三峡库区内重要的产卵江段。了解三峡库中和库尾江段产漂流性卵鱼类的早期资源状况及其对水文和水温特征的响应关系,对于采取适宜的保护措施促进鱼类早期资源的恢复具有重要的意义。本文通过2017—2020年5—7月在库中涪陵断面进行的逐日早期资源监测,了解三峡库中和库尾江段产漂流性卵鱼类早期资源的种类组成、产卵规模、产卵场分布及其年际变动特征,并采用随机森林(RF)模型,分析鱼类逐日产卵规模变动与水文、水温格局及其过程的关系,辨识影响不同种类产卵规模变动的关键水文及水温要素。结果表明:2017—2020年共采集到漂流性鱼卵21种,包括长江上游特有鱼类鱼卵3种;以圆筒吻鮈Rhinagobio cylindricus、蒙古鲌Chanodichthys mongolicus、贝氏(?)Hemiculter bleekeri等6种鱼类的鱼卵为主,占总采集鱼卵数的88.76%;各年的鱼卵规模分别为83.04×108、14.29×...  相似文献   

5.
We present a set of river management tools based on a recently developed method for estimating the amount of salmon spawning habitat in coarse‐bedded rivers. The method, which was developed from a mechanistic model of redd building by female salmon, combines empirical relationships between fish length, redd area, and the sizes of particles moved by fish during spawning. Model inputs are the grain‐size indices D50 and D84 and an estimate of female fish length, which is used to predict the size of the redd that they will build and the size of the largest particle that they can move on the bed. Outputs include predictions of the fraction of the bed that the fish can use for redd building and the number of redds that they can build within the useable area. We cast the model into easy‐to‐use look‐up tables, charts, an Excel worksheet, a JavaScript web applet, and a MATLAB user interface. We explain how these tools can be used in a new, mechanistic approach to assessing spawning substrates and optimizing gravel augmentation projects in coarse‐bedded rivers. © 2016 The Authors. Earth Surface Processes and Landforms published by John Wiley & Sons Ltd.  相似文献   

6.
Static bioassays were conducted at 20.5£C and the hardness of 260 ppm as CaCO3 with zinc sulphate and nickel sulphate using Puntius sophore, Rasbora daniconius neilgeriensis, Channa punctatus and Lebistes reticulates. The 96 h LC50 values for all four fish species ranged from 29.88 to 54.95 £ of zinc and 13.57 to 48.83 £ of nickel. Channa punctatus were consistently more sensitive to zinc, while Puntius sophore were to nickel. The behavioural changes, such as loss of equilibrium and shoaling nature, swimming with their bellies upwards and irregular opercular movement were noted.  相似文献   

7.
The toxicity of phenol, pentachlorophenol and Na-pentachlorophenate to Cypris subglobosa is determined in the batch experiment for 96. h. In the sequence mentioned, the LC50,96h of the three substances are 71.8, 6.56 and 4.41 mg/l, respectively. In the range of 6 … 95 h the mean survival time shows a negative regression to the toxicant concentration, which, however, is not significant statistically for Na-pentachlorophenate. In agreement with this, the LC50 for 12, 24, 48, 72 and 96 h of exposure shows a significant decrease. Due to the comparatively high LC50,96h for pentachlorophenol and Na-pentachlorophenate for C. subglobosa about in comparison with fish, the species is little suited to the toxicological investigation of wastewater.  相似文献   

8.
Exposure against two ppm chromium from K2Cr2O7 for 1 to 7 months produced increasing accumulation of chromium in the liver, kidneys, brain and bone of the freshwater fish Mystus vittatus. In gonads, the accumulation of chromium was progressive for the first 5 months (i.e., April to August), but not in the subsequent 2 months. After 4 months exposure, fish transferred to chromium–free freshwater had a significant depletion of chromium from all tissues at 1, 2 and 3 months.  相似文献   

9.
The tolerance level of the fertilizer NPK for the freshwater siluroid Mystus M. vittatus (BL .) and histopathological changes in the gill of this fish against two concentrations of NPK were investigated. It was found that at 400 ppm (24 h) mortality in the fish started, and at 700 ppm (96 h) all the fish died. Hence the concentration of NPK at 400 ppm was taken as an experimental concentration for this fish. Curling towards the upperside in the secondary lamellae resulting in the joining with each other and profuse mucus secretion and subsequent death of 40% of the fish (96 h) were observed. At 700 ppm (96 h) NPK, which is far higher than the highest tolerance limit of the fish, all the fish died. Profuse mucus secretion, formation of subepithelial spaces, sloughing of epithelial layer, leaving only the rows of pilaster cells, resulting in the death of the fish, were observed. The mortality of the fish was due to asphyxiation and damage in the gill tissues resulting in the alteration in the respiratory and osmoregulatory metabolism.  相似文献   

10.
Static bioassay tests were carried out on Channa punctatus Bloch in the laboratory after renewal of concentrations every 24 h. The LC50 values and 95 per cent confidence limits were 45.21 (39.62 … 51.11) ppm at 12 h and 29.8 (27.49 … 31.95) ppm of Zn at 96 h of exposure. The actual dissolution values of zinc were also determined in test solutions using an atomic absorption spectrophotometer. Behavioural changes were the higher rate of opercular movements, surfacing, loss of equilibrium and haemorrhage near mouth and caudal fin. The LT50 values indicate that survival was increased with decreasing Zn concentration. The histopathological changes observed due to Zn toxicity in the gills have been discussed in relation to fish mortality.  相似文献   

11.
Effects of acute cadmium poisoning on survival, its residual values and histopathology in certain organs of a freshwater airbreathing fish, Channa punctatus (BLOCH ) were investigated. The threshold concentration, MATC and LC50 values obtained from 96 h static bioassay, revealed that Channa is more susceptible to cadmium ions at higher temperature. The atomic absorption spectrophotometric determination of cadmium residues differed significantly in organs of specimens having survived and died after 96 h of exposure. The gill accumulate the highest amount of cadmium, the liver accumulated a slightly smaller amount than the gill, while the kidney accumulated the least. The histopathological lesions subjected to sublethal (5.2 mg/l Cd) and lethal (8.4 mg/l Cd) concentrations of cadmium were: detachment and rupture of lamellar epithelium, collapse of pillar cells and hypertrophy in mucus producing gland cells in the gill; vacuolization and coagulative necrosis in hepatic cells of the liver; and expansion, necrosis and accumulation of cellular debris in renal tubules of the kidney. The probable causes for death of fish due to cadmium ions have been discussed.  相似文献   

12.
Exposure of Channa striatus over 24 h to 40% and 80% of the LC50;24h of the three pyrethroids cypermethrin, permethrin, and fenvalerate did not cause any significant changes in the levels of total protein and free amino acids in the liver and muscle tissues of the fish. After 96 h exposure, the protein amino acid levels are altered significantly.  相似文献   

13.
Monocrotophos (0,0-dimethyl-0-(1-methyl-2-N-methyl-carbamoyl)-cis-vinylphosphate) as an active component of some insecticides is highly soluble in water at a low hydrolysis rate: half-time at 2 mg/l, pH = 7.0 and 38 °C 23 d. Individuals of the following fish species (individual weight in g) were kept during the static bioassay for 6… 72 h at 40 mg/l monocrotophos in the medium: Cyprinus carpio (70), Carassius carassius (45) and Leucaspius delineatus (1.5), and then the concentration of monocrotophos was gas-chromatographically determined in different tissues. Initially, till the 24th hour a high accumulation in the gills took place (in mg/kg fresh weight: Cyprinus 0.49, Carassius 3.88), then the concentration remained uniform or slightly falling here, whereas in the other organs (digestive organs, muscle, gonads) a further accumulation up to 1 mg/kg occurred. Leucaspius was investigated only as whole individuals, and after 72 h their fresh weight had increased to 6.96 mg/kg. The rates of accumulation decrease in the following order: Cyprinus: digestive organs, gills, muscle; Carassius: gills, digestive organs, gonads, muscle.  相似文献   

14.
2014年对丹江口水库上游四大家鱼等产漂流性卵鱼类进行早期资源的现状调查,结果表明丹江口水库上游现有产漂流性卵鱼类共14种,以银鮈、蛇鮈、花斑副沙鳅、、蒙古鲌等鱼类为主,四大家鱼卵64粒,仅占卵数的1.02%.与1977年、1993年历史调查的产漂流性卵鱼类早期资源数据相比,产卵场数量减少6处,产卵场的规模和位置发生显著变化.丹江口水库上游产漂流性卵鱼类产卵总量下降明显,从1977年的近1745.86亿粒(尾)下降到2014年的455502.76万粒(尾);其中四大家鱼卵苗径流量下降幅度最为显著,从171.27亿粒(尾)下降到4295.22万粒(尾),四大家鱼卵苗径流量占鱼类卵苗总径流量的比例也从9.81%下降到了0.94%以下.  相似文献   

15.
A total of 2588 adult migratory brown trout (Salmo trutta lacustris) have been tagged from 1964 to 1974 on their spawning sites, in tributaries of the western part of Lake Geneva (Switzerland). Most of the fish (96.2%) recaptured during spawning seasons in the following years have been found in the same river where they had previously been caught. Most tagged fish recaptured by fishermen were found in the western part of the Lake, near their home rivers. This tendency is very strong in autumn and winter. It still remains highly significant in spring and summer.

Dédié à Wolfgang Geiger  相似文献   

16.
The 96h LC50 of the water soluble fraction (WSF) of natural gas condensate from the Sable Is., N.S. area to Fundulus heteroclitus was between 4 and 5 μg · ml−1 naphthalene equivalents (initial concentration). Behavioural responses in the fish to sub-lethal concentrations of WSF were similar to those recorded for crude and fuel oils. Induction of the hepatic mixed function oxidase enzyme enthoxyresorufin O-de-ethylase (EROD) in sexually immature fish occurred at WSF concentrations below the LC50, but during the prespawning period no induction occurred. EROD activity in unexposed fish showed seasonal variation consistent with differences in its inducibility.  相似文献   

17.
Testing Effluents of the Textile Refining Industry with Biological Methods The environmental problems caused by the manufacture of finished textiles involve a long chain of individual processes. This “textile chain” includes very diverse enterprises of varied size and structure. The textile refiners occupy a key position in the “textile chain”. On the one hand, this is due to their use of an obscurely large number of chemicals which can end up in the wastewater as well as in the textile products. On the other hand, this key role of the textile refining industry is based on their central position between the preproduction stage and the consumers. This study dealt with the textile refining industry's wastewater. As measured by volume and contents of its wastewater, this industry can be counted among the major industrial plants which discharge into municipal wastewater treatment plants. German wastewater legislation includes the provision that substances which are toxic, persistent, capable of accumulating, carcinogenic, fetotoxic or mutagenic be kept out of natural waters as well as technically possible (Wasserhaushaltsgesetz WHG). Several biotest methods for examining the effect of the substances contained in the wastewater were incorporated into the appendix of the German wastewater regulation (Rahmenabwasser-Verwaltungsvorschrift based on § 7a WHG). The aim of this study was to show, with the aid of biotest methods, how strongly the wastewater of textile refining companies is polluted as compared to other known industrial branches and to what degree the pollution of these wastewaters is eliminated by the treatment in wastewater treatment plants. Finally, we experimented to find out which biotest methods were suited for the examination of these wastewaters. The study's results show that the ecotoxicity of the textile refining industry's wastewater was only extraordinary high in isolated cases as compared to other examined branches of industry. The textile wastewaters exhibit values of GL = 3 to GL = 96 in the luminescent bacteria test, GD = 1 to GD = 192 (with one exception of GD > 30000) in the daphnia test and GF < = 2 to GF = 32 in the fish test. It turned out though, that a large number of the samples from the textile refining companies (27%) reacted mutagenically in the Ames test in their native state. Consecutive tests for chromosomal aberrations (V79 hamster cell test) also showed mutagenic potential in five out of nine native samples. The employed testing methods with fish, daphnia and luminescent bacteria demonstrate a higher sensitivity of the luminescent bacteria and/or the daphnia as opposed to the fish in most cases. As the fish test is controversial anyway on the grounds of animal protection, a replacement of the fish test by these other tests should be aimed at: on account of the different end points of the luminescent bacteria and the daphnia test, a combination of these tests appears most sensible.  相似文献   

18.
The haematological, biochemical, and enzymological alterations produced on exposure of Channa punctatus to LC50 (11.2 mg/L) for 96 h and to a sublethal concentration (1.12 mg/L) of cadmium (Cd2+) for 15, 30, 60, and 120 days have been studied. The fish were hypoglycemic and hypolactemic. The pyruvate content of blood and liver decreased in acute and all stages of chronic exposure except for 30 days where significant increase was recorded. Depletion was noted in the total protein and glycogen content of liver and muscle, and the level of lactate in liver in the two types of exposure. The levels of muscle lactate and pyruvate increased in both exposures. The activities of hexokinase, glucose-6-phosphatase, and lactate dehydrogenase decreased in liver after 96 h and all stages of chronic exposure except 30 days. The activities of hexokinase and lactate dehydrogenase in muscle and glutamate-oxalacetate transaminase and glutamate-pyruvate transaminase in serum, liver, and muscles increased in all exposures. The activities of succinate and malate dehydrogenases in liver and muscles decreased after acute and all stages of chronic exposure. The above results suggest that glycolysis and gluconeogenesis were impaired in liver but glycolysis prevailed in muscle. Aerobic oxidation is adversely affected in metal exposed fish, while proteolysis was increased.  相似文献   

19.
Ripe females of whitefish (Coregonus lavaretus) of Lake Constance were caught at spawning time on their known spawning sites. Fish were examined for their size, gill-raker number, and for their egg dry weight. On the basis of their spawning sites as well as on correlation between body weight and egg weight, fish were allocated at one of the two sympatric populations: nearshore spawning Gangfisch or pelagic spawning Blaufelchen. The two fish samples were examined electrophoretically for the enzyme products of 37 genetic loci. Blaufelchen and Gangfisch were genetically identical (Nei's unbiased genetic distance D = 0.000) when all fish samples were included into the analysis. However, some genetic differentiation (D = 0.001) was evident after excluding the fish whose data points on body weight versus egg weight overlapped. Additional enzyme systems or some other genetic methodology should be used to provide more information on the genetic status of the two whitefish forms in Lake Constance.Contribution No. 8 in the Program of Joint Investigation of Holarctic Fishes among the Russia, Canada, Finland, and Poland  相似文献   

20.
杨志  唐会元  龚云  朱迪  赵娜 《湖泊科学》2018,30(3):753-762
鱼类作为河流生物的重要组成部分,其在干支流之间的迁徙或移动不仅是常见的,而且通常与鱼类的繁殖活动相联系.金沙江下游支流作为金沙江下游河流网络的重要组成部分,分布有较为丰富的集合生境以及较高的鱼类物种多样性.研究鱼类在金沙江下游干支流的产卵迁徙对支流鱼类群聚结构变动的影响以及支流生境维持对区域鱼类种群维系的意义,对金沙江下游干支流鱼类的保护具有重要的意义.本文拟选择黑水河下游江段作为典型研究区域,通过2014年在该区域的逐月渔获物调查,采用聚类分析、基于距离的线性模型以及基于距离的冗余分析等多种多元分析方法,确定黑水河下游群聚结构的逐月变动是否严重依赖于鱼类在干支流之间的产卵迁徙以及黑水河下游自然生境的维持对区域鱼类种群的维系是否具有重要的意义.结果表明:黑水河下游鱼类群聚结构在金沙江雨季和旱季显著分离的同时,表征这种分离的8种指示种的性成熟个体丰度也在雨季和旱季间发生不同程度的变动.7种指示种鱼类性成熟个体丰度的变动能够解释黑水河下游鱼类群聚结构变动77.20%的变异,其中齐口裂腹鱼、大鳞副泥鳅和犁头鳅性成熟个体丰度的变动是影响黑水河下游鱼类群聚结构变动的3个最显著的因素,7种指示种鱼类在干支流的产卵迁徙对黑水河下游鱼类群聚结构的变动造成了明显的影响.研究表明:在金沙江干支流严重水电开发背景下,维持黑水河现有的自然生境面积对区域鱼类种群的维持具有重要意义.为实行上述目标,建议在白鹤滩水电站蓄水运行后,拆除黑水河的部分小型水坝,并采取其他河流再自然化措施以维持黑水河现有的自然生境面积.  相似文献   

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