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1.
研究了三个蛋白水平(22%、25%、28%)和五个L-肉碱水平(0、200、400、600、800mg/kg)下尼罗罗非鱼生长和饲料利用率。结果表明,随着饲料中蛋白水平的提高,罗非鱼幼鱼的终体重、增重率(WGR)和特定生长率(SGR)呈上升趋势,而饲料系数(FCR)和蛋白质效率(PER)均随饲料蛋白水平的升高而显著下降(P<0.05);同等蛋白水平下,终体重、WGR和SGR均在L-肉碱添加量为200mg/kg时最高,不添加L-肉碱组最小;在添加400mg/kgL-肉碱时FCR最小,而PER最大,与不添加L-肉碱组比较,差异均显著(P<0.05)。说明饲料中蛋白含量和L-肉碱添加量能显著影响罗非鱼幼鱼的生长和饲料利用,而外源性L-肉碱可明显促进罗非鱼生长,提高饲料转化效率。  相似文献   

2.
在以豆粕、酪蛋白、鱼粉(质量分数分别为33%、17%、15%)为蛋白源的基础饲料中分别添加质量分数0、0.4%、0.8%、1.2%、1.5%和2.0%的胆固醇,制成6种等氮等能饲料,养殖初始体质量为(24.00±0.19)g的军曹鱼幼鱼(Rachycentron canadum)8周,研究胆固醇对其生长、体成分、血液生化指标和脂代谢相关酶活性的影响。结果表明:添加低水平胆固醇对军曹鱼生长有改善作用,而高水平胆固醇对生长有抑制作用,与对照组比较,增重率(WGR)(P0.05)、成活率(SR)(P0.05)、饲料系数(FCR)(P0.05)在0.4%组较佳,随添加量的增加,各组幼鱼有增重率、成活率降低,饲料系数增大趋势。幼鱼肝体比、脏体比、肝脏病变率有增大趋势。添加胆固醇后,全鱼、肝脏和肌肉中粗脂肪0-0.4%组有上升趋势,0.4%-2.0%组有下降趋势;鱼体粗蛋白有上升趋势,肝脏中胆固醇有先降后升趋势,但水分和灰分的变化不大(P0.05)。血清中总胆固醇(TC)、低密度胆固醇(LDL-C)有上升趋势。肝脏中激素敏感性脂肪酶(HSL)有先升后降趋势。在以豆粕为主要蛋白源的饲料中添加少量胆固醇(0.4%组)可一定程度上改善军曹鱼幼鱼生长,影响机体脂代谢,但过多添加会抑制生长,并造成肝脏病变。以SR为判据,饲料中军曹鱼对胆固醇最适需要量为0.566%。  相似文献   

3.
在饲料中分别添加3.0×108kg-1(A组)、1.5×108kg-1(B组)、1.0×108kg-1(C组)的嗜酸小球菌,制成3种实验饲料,以不添加嗜酸小球菌(D组)作为对照,饲养日本鳗鲡60d,以研究嗜酸小球菌对日本鳗鲡生长和免疫的影响。测定了增重率、饵料系数、成活率、血清中一氧化氮含量以及人工感染后的死亡率,并通过电镜观察了小肠壁细胞的微绒毛结构。结果表明A、B、C组和对照组的相对增重率分别为42.31%、75.42%、89.65%和40.32%,饲料系数分别为1.93、1.12、0.98和1.58,C组的相对增重率显著高于其他组,饵料系数则显著低于其他组,成活率也显著提高。电镜观察发现C组和B组的鳗鲡小肠细胞微绒毛排列整齐、细而长,吸收面积增大,从而提高了对饲料利用率,D组鳗鲡小肠细胞的微绒毛稀疏、短,排列不均匀。血清中NO的含量A、B、C组分别比对照组提高了23%、42%和73%。C组人工感染后的死亡率显著低于其他组。实验结果表明,在鳗鲡饲料中添加嗜酸小球菌可以显著促进鳗鲡的生长,降低饵料系数,增强其免疫力。  相似文献   

4.
研究0、300、600、900、1 200、1 500 mg/kg 6个L-肉碱添加水平对牙鲆(Paralichthys olivaceus)幼鱼生长、生化组成和血液指标的影响。结果表明:添加量为1 200 mg/kg的处理组牙鲆幼鱼的增重率和特定生长率显著提高,饲料系数显著降低(P<0.05);随着饲料中L-肉碱含量的增加,肝指数呈现下降趋势,且在添加量为900~1 500 mg/kg时,各组较对照组差异显著(P<0.05);饲料中添加L-肉碱显著降低各实验组脏体比(P<0.05),而对各处理组间存活率和肥满度均无影响(P<0.05);肌肉和肝脏中的脂肪含量与L-肉碱添加量呈负相关,当添加量为1 200~1 500 mg/kg时,较对照组差异显著(P<0.05);肌肉中的蛋白含量与L-肉碱添加量呈正相关,但各组差异不显著(P<0.05);饲料中添加L-肉碱降低血清中的谷草转氨酶、胆固醇、甘油三酯和尿素氮含量,且添加量1 200 mg/kg组各指标含量均显著低于对照组(P<0.05);各处理组的血糖含量较对照组均有所增加,其中1 200 mg/kg组血糖含量较对照组提高41.6%(P<0.05);血清中总蛋白和低密度脂蛋白均随着L-肉碱添加量的增加而呈下降趋势,高密度脂蛋白却得以提升,但差异均不显著(P<0.05)。分析认为,在本实验条件下,饲料中添加L-肉碱可促进牙鲆幼鱼生长,且降脂效果显著。建议L-肉碱最适添加量为1 200 mg/kg。  相似文献   

5.
用添加质量分数为0.00%(对照组)、0.02%、0.05%、0.20%、0.50%褐藻酸寡糖(alginate oligosaccharides,AOS)的基础饲料饲养初始体质量为(17.61±0.16)g的大菱鲆(Scophthalmus maximus)幼鱼10周,研究AOS不同添加量对大菱鲆幼鱼生长、血液学指标、非特异性免疫的影响。结果表明:与对照组相比,在4个不同添加量组中,增重率、特定生长率差异无统计学意义(P0.05);各组血液中白细胞含量均提高,差异有统计学意义(P0.05),血红蛋白、红细胞压积及红细胞数差异无统计学意义(P0.05);血清中谷丙转氨酶、谷草转氨酶、总胆固醇、甘油三酯、高密度脂蛋白含量降低,但差异无统计学意义(P0.05);0.02%、0.05%、0.20%组的血清中酸性磷酸酶活力高于对照组,差异有统计学意义(P0.05),但超氧化物歧化酶、溶菌酶活力与对照组差异无统计学意义(P0.05),其中0.05%、0.20%组碱性磷酸酶明显高于对照组(P0.05);添加AOS组肝脏中的酸性磷酸酶活力以及超氧物歧化酶SOD活力与对照组相比差异无统计学意义(P0.05);攻毒试验发现,大菱鲆幼鱼对腹水病的疾病抵抗力随添加量的增加而升高。在本实验条件下,饲料中添加AOS对大菱鲆幼鱼生长性能和血液学指标并无显著影响,但可提高其非特异性免疫功能。建议添加量为质量分数0.05%。  相似文献   

6.
一种复方中草药饲料添加剂在红笛鲷网箱养殖中的应用   总被引:1,自引:1,他引:0  
将黄芪、当归、甘草和山楂等粉碎后按一定比例配伍制成复方中草药饲料添加剂后,按质量分数0.5%、1.0%、1.5%和2.0%添加到基础饲料中,连续投喂红笛鲷(体重234.95±19.26 g,体长24.76±1.42 cm)56 d,研究复合中草药制剂对红笛鲷生长、成活、免疫和抗病力的影响。结果表明,基础饲料中添加本复方中草药饲料添加剂能够显著促进红笛鲷的生长,增强其血清中的抗菌活力和溶菌酶活力,提高红笛鲷成活率及其对溶藻弧菌的免疫保护率,其中以添加1.5%(质量分数)和连续饲喂28 d效果为最佳(P<0.01)。  相似文献   

7.
抗菌肽对凡纳滨对虾生长和机体免疫的影响   总被引:1,自引:0,他引:1  
基础饲料(对照组)中添加10g/kg抗菌肽制剂喂养体重0.10±0.01g、体长2.17±0.11cm的凡纳滨对虾(Penaeus vannamei)30d,进行10d的水浴攻毒(鳗弧菌10~8/mL),探讨饲料中添加抗菌肽对凡纳滨虾生长、成活率、免疫保护率,以及肌肉中溶菌酶活性、超氧化物歧化酶活性、总抗氧化能力的影响。结果表明:饲料中添加抗菌肽可显著提高凡纳滨对虾相对增重率、成活率和对致病菌的免疫保护率(P<0.05),但对饲料系数无显著影响(P>0.05);未受外界微生物刺激时,饲料中添加抗菌肽对凡纳滨对虾总抗氧化能力有促进作用(P<0.05),受外界微生物刺激后,饲料中添加抗菌肽喂养凡纳滨对虾可提高对虾超氧化物歧化酶活性(P<0.05)和总抗氧化能力(P<0.01)。因此饲料中添加抗菌肽喂养凡纳滨对虾可提高其机体非特异性免疫。  相似文献   

8.
复方中草药对吉富罗非鱼生长及肠道菌群的影响   总被引:1,自引:0,他引:1  
将A、B、C三种复方中草药分别按质量分数1.5%的比例添加到罗非鱼商品饲料中,喂养初始体重约16.58±0.48g的吉富罗非罗28d,通过测定罗非鱼的增重率、饲料系数、肠道菌群等指标,研究三种复方中草药制剂对罗非鱼生长性能及肠道菌群的影响。结果显示:三种复方中草药制剂对罗非鱼的增重率、成活率、饲料系数等生长性能指标虽无显著影响(P>0.05),但与对照组相比,添加1.5%的复方中草药制剂C可使吉富罗非鱼的增重率提高10.82%,饲料系数降低4.71%,蛋白质效率提高15.68%;三种复方中草药制剂均可显著促进罗非鱼肠道细菌、双歧杆菌、乳酸杆菌的生长(P<0.05),C方中草药制剂还可显著抑制大肠杆菌生长(P<0.05)。结果表明,C方中草药制剂可显著促进罗非鱼肠道中乳酸杆菌等有益菌群的增殖,抑制大肠杆菌生长,从而有效促进罗非鱼生长。  相似文献   

9.
谷氨酰胺对肉仔鸡早期生长和组织器官发育的影响   总被引:5,自引:0,他引:5  
在肉仔鸡基础日粮中添加谷氨酰胺进行21d的试验,观察了其对肉仔鸡早期生长性能和组织器官发育的影响。试验表明,在肉仔鸡饲料中添加适宜的谷氨酰胺,有利于提高肉仔鸡的体重、日增重、饲料报酬和成活率,添加量为0.8%时效果最好,且谷氨酰胺添加量为0.8%时可明显提高21日龄时法氏囊、脾脏、肝脏和胰腺的重量以及法氏囊指数。  相似文献   

10.
复合酸化剂对鲤生长及消化酶活性的影响   总被引:1,自引:0,他引:1  
在鲤鱼(Cyprinus carpio)基础饲料中,分别添加不同梯度的复合酸化剂(质量分数分别为0、0.2%、0.3%),研究复合酸化剂对其生长和消化酶活性的影响。结果表明:1)添加复合酸化剂显著(P<0.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标;2)添加质量分数0.3%的复合酸化剂时,鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P<0.05),酸化剂组的淀粉酶和脂肪酶活性有升高的趋势,但与对照组差异不显著;3)鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。综上所述,鲤鱼饲料中添加以柠檬酸、乳酸、磷酸等为主的复合酸化剂,可显著提高鲤鱼肝胰脏、前肠和中肠的蛋白酶活性,促进鲤鱼生长。  相似文献   

11.
A simple method for spiking formulated feed with domoic acid (DA) was developed in this study. DA feed was prepared by mixing 0.15 mL 100 μg mL-1 DA with 0.1 g formulated feed, and drying the mixture at room temperature for 2 h. The prepared DA feed contained 0.19 pg DA per particle. Of the added DA, 46.72% was retained in the feed. Relatively high DA retention (about 50%) was recorded after DA feed was soaked in water for 2h. Exposure to DA feed for 7d did not cause the increase of tissue DA level of adult king scallop (Pecten maximus) significantly in 60 d. The increase of their gonad index after DA exposure was not significantly different from the control. No significant change in DA level was found in spermary, ovary or fertilized eggs after DA exposure. These results indicated that DA excretion may be more efficient than DA accumulation under the current experimental conditions, and the mechanism of domoic acid incorporation in P. maximus may involve intracellular biotransformation.  相似文献   

12.
总结黑水虻的生物特性、营养成分含量及其在水产饲料中的应用,简述黑水虻幼虫不同产品形式(干虫/虫粉、脱脂虫粉、虫油、鲜虫/虫浆)在草食性、杂食性、肉食性和甲壳动物饲料中的应用进展,阐述在水产动物饲料中适量添加黑水虻可提高所饲动物的生长性能,过量添加则引起动物发生病理性变化,综述在不同水生动物中黑水虻的最适添加量,提出黑水虻在产业化生产及水产饲料中的应用前景及问题。  相似文献   

13.
在不同饵料条件下,对双齿许水蚤(Schmackeria dubin)的清滤率(mL.只-1.h-1)、滤食率(细胞.只-1.h-1)和摄食率(mg.只-1.h-1)进行了测定。结果表明:以湛江等鞭金藻(Isochrysis zhanjiangensis)为饵料,其浓度为15×104~120×104 mL-1时,双齿许水蚤的摄食率达到较大;分别饲以同生物量的牟氏角毛藻(Chaetoceros müelleri)、亚心形扁藻(Platymonas subcordiformis)、湛江等鞭金藻、小球藻(Chlorella sp.)时,饵料为角毛藻和扁藻时双齿许水蚤的摄食率达到较大;饲以同生物量的牟氏角毛藻、亚心形扁藻、湛江等鞭金藻、小球藻、颤藻(Oscillatoria sp.)、小环藻(Cyclotella sp.)六种藻的混合饵料时,双齿许水蚤对湛江等鞭金藻的摄食率和选食率均为最大,分别为0.0458 mg.只-1.h-1和17.1%。可见,饵料浓度、饵料种类、混合饵料对双齿许水蚤的清滤率和摄食率均有显著影响(P<0.05)。  相似文献   

14.
通过对高效液相色谱法(HPLC)测定水产饲料中三聚氰胺含量的方法进行优化,得到最佳色谱条件和前处理方法,建立了水产饲料中三聚氰胺的检测方法。结果表明:以体积比8∶92的乙腈、庚烷磺酸钠离子对试剂为流动相,柱温40℃,流速1.2 mL/min,以波长236 nm检测;以提取液为18 mL50%(φ)乙腈水+1 mL乙酸锌+1 mL1mol/L盐酸,淋洗液3 mL水和3 mL甲醇,洗脱体积6 mL作样品前处理。三聚氰胺加标回收率为85%~100%,相对标准偏差(RSD)小于5%,线性范围为0.2 mg/L~5.0mg/L,相关系数0.999 8,方法检出限为0.2 mg/kg。  相似文献   

15.
研究了温度22、25、28、31和34℃下奥尼罗非鱼(Oreochromis niloticus×O.aureus)仔稚鱼生长、饲料利用和消化酶活力。结果表明:1)不同温度对奥尼罗非鱼仔稚鱼生长有显著影响(P<0.05),温度为31℃时,仔稚鱼生长速度和绝对增重率最高,分别为0.08cm/d和0.035g/d;温度为22℃时最低,仅0.06cm/d和0.020g/d。2)饲料系数在28℃时最低,仅1.90;22和34℃时较高,达2.14和2.17,各组差异显著(P<0.05)。3)在仔稚鱼发育和存活率上,当温度在22~34℃时,温度越高,仔稚鱼发育越好;各组仔稚鱼的存活率介于93.7%~94.7%,差异不显著(P>0.05)。4)在消化酶活力上,胃蛋白酶、胰蛋白酶和淀粉酶活力随温度升高而升高,均在34℃时活力最高;脂肪酶活力在31℃时达到最高,22℃时活力最低。  相似文献   

16.
Seasonal and sexual variations as well as the effect of dry feed supplement on total drinking water intake and its utilization were observed in mithun (Bos frontalis)-a semi-wild animal found in North Eastern Hill Region (NEHR) of India. In a completely randomized design, twelve adult mithuns (B. frontalis) as per their sex and body weight were assigned in two different rearing systems (free grazing and free grazing with dry concentrate feed supplementation), and ten growing male mithuns as per their body weight assigned in two different levels of dry concentrate feed supplementation (1.0 kg and 2.0 kg dry concentrate feeds on green forage based diet) and in two different seasons (summer and winter). It was observed that the environmental temperature had a significant effect on drinking water intake by mithuns. Drinking water consumption (per unit of body weight) was significantly (P < 0.05) higher in summer than in winter. Supplementation of concentrate feed on free grazing animals resulted in increase in water consumption. Total water consumption (drinking as well as performed water) was found to be 15.18 litres per 100 kg body weight by growing mithun. Feed dry matter and digestible nutrient intakes by growing mithun were observed to be increased with the increase of supplementation of dry concentrate feed. Roughage to concentrate ratio did not affect the nutrient digestibility. Mithun calves drank an average of 4.30 litres water for each kg of dry matter intake. Metabolic water was significantly (P<0.01) increased with the increase of supplementation of concentrate feed whereas water turn over, which depends upon the body weight of the animals, did not differ significantly on offering of lower or higher level of dry feed. Faecal water loss of growing mithun was decreased with the increase in intake of concentrate feed and was estimated to be 33~46% of total water intake. Excretion of water through faeces of mithun was about 3.8% of body weight. It could, therefore, be inferred that water intake by mithun varied with seasons, rearing systems and dry feed consumption. As far as the water nutrition is concerned, it is needed to give an attention while feeding mithun in summer with dry feed supplementation under semi-intensive system of rearing.  相似文献   

17.
Seasonal and sexual variations as well as the effect of dry feed supplement on total drinking water intake and its utilization were observed in mithun (Bosfrontalis) - a semi-wild animal found in North Eastern Hill Region (NEHR) of India. In a completely randomized design, twelve adult mithuns (B. frontalis) as per their sex and body weight were assigned in two different rearing systems (free grazing and free grazing with dry concentrate feed supplementation), and ten growing male mithuns as per their body weight assigned in two different levels of dry concentrate feed supplementation (1.o kg and 2.0 kg dry concentrate feeds on green forage based diet) and in two different seasons (summer and winter). It was observed that the environmental temperature had a significant effect on drinking water intake by mithuns. Drinking water consumption (per unit of body weight) was significantly (P 〈 0.05) higher in summer than in winter. Supplementation of concentrate feed on free grazing animals resulted in increase in water consumption. Total water consumption (drinking as well as performed water) was found to be 15.18 litres per 100 kg body weight by growing mithun. Feed dry matter and digestible nutrient intakes by growing mithun were observed to be increased with the increase of supplementation of dry concentrate feed. Roughage to concentrate ratio did not affect the nutrient digestibility. Mithun calves drank an average of 4.30 litres water for each kg of dry matter intake. Metabolic water was significantly (P〈0.01) increased with the increase of supplementation of concentrate feed whereas water turn over, which depends upon the body weight of the animals, did not differ significantly on offering of lower or higher level of dry feed. Faecal water loss of growing mithun was decreased with the increase in intake of concentrate feed and was estimated to be 33 - 46 % of total water intake. Excretion of water through faeces of mithun was about 3.8 % of body weight. It could, there  相似文献   

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