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1.
The toxicity of seven major HAB (harmful algal bloom) species/strains, Prorocentrum donghaiense, Phaeocystis globosa, Prorocentrum micans, Alexandrium tamarense (AT-6, non-PSP producer), Alexandrium lusitanicum, Alexandrum tamarense (ATHK) and Heterosigma akashiwo were studied against rotifer Brachionus plicatilis under laboratory conditions. The results show that P. donghaiense, P. globosa, P. micans, A. tamarense (AT-6), or A. lusitanicum could maintain the individual survival and reproduction, as well as the population increase of the rotifer, but the individual
reproduction would decrease when exposed to these five algae at higher densities for nine days; H. akashiwo could decrease the individual survival and reproduction, as well as population increase of the rotifer, which is similar
to that of the starvation group, indicating that starvation might be its one lethal factor except for the algal toxins; A. tamarense (ATHK) has strong lethal effect on the rotifer with 48h LC50 at 800 cells/mL. The experiment on ingestion ability indicated by gut pigment change shows that P. donghaiense, P. globosa, P. micans, A. tamarense (AT-6) and A. lusitanicum can be taken by the rotifers as food, but A. tamarense (ATHK) or H. akashiwo can be ingested by the rotifers. The results indicate that all the indexes of individual survival and reproduction, population
increase, gut pigment change of the rotifers are good and convenient to be used to reflect the toxicities of HAB species.
Therefore, rotifer is suggested as one of the toxicity testing organisms in detecting the toxicity of harmful algae.
Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-208), National Natural Science
Foundation of China (No. 40821004, U0733006) and National Basic Research Program of China (973 Program, No. 2001CB409700) 相似文献
2.
We investigated the effect of CO2 enrichment and initial inoculum density on competition between Skeletonema costatum and Heterosigma akashiwo, two common algae seen in algal blooms. The initial inoculum density (0.2×104, 0.4×104, 0.8×104 cells/ml) had a significant effect on population growth and competition between H. akashiwo and S. costatum. The time required for population growth to reach the exponential growth phase and stationary phase decreased significantly
as the initial density increased. When the two species were cultured together, S. costatum tended to outcompete H. akashiwo, especially when present at higher initial ratios. CO2 enrichment (5 000 μl/L CO2) increased the maximum population density and carrying capacity of H. akashiwo but decreased these measures for S. costatum. Thus, CO2 enrichment favored the growth of H. akashiwo over S. costatum. 相似文献
3.
A laboratory experiment was conducted to assess the bioaccumulation of Pb2+ and its effects on growth, morphology and pigment contents of Spirulina (Arthrospira) platensis. The specimen cultured in Zarrouk liquid medium was treated with various initial metal concentrations (0, 5, 10, 30, 50 and
100 μg mL−1). The growth of S. platensis was adversely affected by Pb2+ at high concentrations (30, 50 and 100 μg mL−1). However, at low concentrations (5 μg mL−1), Pb2+ could stimulate its growth slightly. The pigment contents (chlorophyll α and β carotene) were decreased in a dose-dependent
manner. The highest reductions (67% and 53% respectively in chlorophyll α and β carotene) were observed in 100 μg mL−1 treatment group. The LC50 (96 h) of Pb2+ was measured as 75.34 μg mL−1. Apart from a few cases of filament breakages at elevated concentrations (50 and 100 μg mL−1), morphological abnormalities are not specific. Metal bioaccumulation increased with Pb2+ concentrations, but decreased with exposure time. The maximum accumulated amount was 188 mg g−1 dry weight. The bioconcentration factor (BCF) reached to a peak at day 2, followed by a gradual reduction for all the exposure
concentrations. S. platensis is able to tolerate considerably high Pb2+ concentrations. Consequently it can be used as a potential species to remove heavy metal from contaminated waters. 相似文献
4.
A bloom of the dinoflagellate Scrippsiella trochoidea was detected for the first time in inner Tolo Harbor, Hong Kong in 2 000. Water samples were collected at eight stations
along a transect passing through a red tide patch for microscopic analysis of phytoplankton composition and high-performance
liquid chromatography (HPLC) analysis of phytoplankton pigments. During the bloom, the density of dinoflagellates was 1.1×106 cells L−1 within the patch and 8.6×105 cells L−1 outside the patch where the phytoplankton community was dominated by diatoms. After the bloom the S. trochoidea began to decrease in density and was replaced by diatoms as the dominating bloom-causing organisms at all stations, and the
density of dinoflagellates at most stations was less than 1.0 × 106 cells L−1. The status of S. trochoidea as the causative species of the bloom was indicated by the presence of peridinin, the marker pigment for dinoflagellates.
The shift from dinoflagellates to diatoms was marked by the decline of peridinin and the prevalence of fucoxanthin. Phytoplankton
pigment markers also revealed the presence of other minor phytoplankton assemblages such as cryptomonads and blue-green algal. 相似文献
5.
Sodium thiosulphate and neutron activation determination of iodine content in samples of 18Sargassum species collected in Guangdong and Guangxi Province in April 1996 showed large differences (0.47×10−3 inSargassum hemiphyllum, 0.56×10−3 inSargassum assimile, and 4.5×10−3 inSargassum vachellianum). Traditional titration analysis and neutron activation analysis for determination of total iodine yielded similar results
showing that certainSargassum species had high capacity to accumulate iodine.
Contribution No. 3162 from the Institute of Oceanology, Chinese Academy of Sciences.
The project was supported by the National Ninth Five-Year Plan of China (No. 96-916-04-01). 相似文献
6.
Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary ecosystems.
We have established a multi-generation cultivation system for zooplankton with which future experiments on the biological
effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Schmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages (eggs to adults). The population exhibited
an average developmental time of 13.6 d in conditions corresponding to the natural environment (water temperature 20°C, salinity
15). A series of experiments were performed to examine copepod egg production and hatching success as functions of food type
and feeding concentration. Results in our study showed that Isochrysis galbana was more favored for the reproduction of copepods than Phaeodactylum tricornutum, and 10×104cells mL−1 was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production
through changes in food concentration have been established, making it feasible to control the start date of exposure experiments
or the timing of the collection of offspring to initiate a new generation. 相似文献
7.
Vibrio harveyi cells (dose—<103 cells mL−1) and extracellular products (ECP; >25 μmg mL−1 of total protein concentration) destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of exposure. Cytopathic effects (CPE) started after 4h of exposure to the bacterial cells, with some granularity
in the cytoplasm, mostly in cells in the outer periphery of the explant growth. At the end of the infection, a considerable
number of nuclei remained attached to the substrate, apparently unaffected. Following exposure to ECP, initial deterioration
was observed at 2 h with the presence of granularity in the cytoplasm of<20% cells, and few cells displayed small vacuoles
around the nuclei. Parallel results were obtained using whole animal experiments, with V. harveyi cells being lethal to nephrops within 24 h. 相似文献
8.
This study deals with contribution of artificial food pellet and natural food to Chinese prawn (Penaeus orientalis) growth in a semiintensive culture pond. The prawn carbon consumption, budget, and the effects of some factors on the budget were investigated. The results showed that 26.2% of P. orientalis growth carbon came from formulated feed at the initial culture stage (when the prawns were 0.06±0.01 g in wet weight), and was 62.5% when the prawns were 9.56±1.04 g. The remaining part of the growth carbon was derived from organic fertilizer and natural food. The highest growth rate occurred at 20×10-3 salinity. Suitable salinity for culturing Chinese prawn was (20-28)×10-3. 相似文献
9.
Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships
between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from
the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations
(LC50 values) were given. Results of measurements indicated that 2 h-LC50 and 12 h-LC50 values of formalin on P. coronatum were 59.00×10−6 and 43.57×10−6, while those of Lugol’s solutions were 90.13 and 67.84×10−6 respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to
kill ciliates in short time. 相似文献
10.
CCC plasmid was isolated from an economically important blue-green alga —Spirulina platensis (1.7×106 dalton from the S6 strain and 1.2×106 dalton from the F3 strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference
in the molecular weight of the CCC DNAs from the two strains differing in form suggests that plasmid may be related with the
differentiation of algal form. This modified method, which does not use any lysozyme, is a quick and effective method of plasmid
isolation, especially for filamentous blue-green algae.
Contribution No. 79 of the Experimental Marine Biology Laboratory and 2153 of the Institute of Oceanology, Academia Sinica.
This research was supported in part by The President of the Chinese Academy of Sciences Foundation. 相似文献
11.
Interactions between Skeletonema costatum (S. costatum) and Prorocentrum donghaiense (P. donghaiiense) were inves-tigated using bi-algal cultures at different concentrations of phosphate (PO4-P) and nitrate/phosphate (N/P) ratios. Experiments were conducted under P-limited conditions and the Lotka-Volterra mathematical model was used to simulate the growth of S. costatum and P. donghaiense in the bi-algal cultures. Both of these two species were inhibited significantly in bi-algal culture. The results of the simulation showed that the inhibitory degree of S. costaum by P. donghaiense was high when the concentration of PO4-P was low (0.1μmolL-1/2 d), but that of P. donghaiense by S. costaum was high with increased PO4-P supply (0.6μmolL-1/2 d). At low concen-tration of PO4-P (0.1μmolL-1/2 d), or high concentration of PO4-P (0.6μmolL-1/2 d) with high N/P ratio (160), the interactions be-tween S. costatum and P. donghaiense were dependent on the initial cell densities of both species. At high concentration of PO4-P (0.6μmolL-1/2 d) with low N/P ratio (25 or 80), S. costatum exhibited a survival strategy superior to that of P. donghaiense. The de-gree of inhibition of P. donghaiense by S. costaum increased with elevated N/P ratio when the medium was supplemented with con-centration 0.1μmolL-1/2 d of PO4-P. The degree of inhibition to P. donghaiense by S. costaum increased with elevated N/P ratio at low concentration of PO4-P (0.1 μmolL-1/2 d). This trend was conversed at high concentration of PO4-P (0.6μmolL-1/2 d). However, the degree of inhibition of S. costaum by P. donghaiense increased with the increased N/P ratio at different PO4-P concentrations (0.1μmolL-1/2 d and 0.6μmolL-1/2 d). These results suggested that both phosphate concentration and N/P ratio affected the competition between S. costaum and P. donghaiense: P. donghaiense is more competitive in environments with low phosphate or high N/P ratio and the influence of N/P ratio on the competition was more significant with lower phosphate concentration. 相似文献
12.
When cultured under certain environmental conditions (25°C, light intensity 80 μmol/m2·s, LD 12/12, in enriched seawater medium with 7×10−4 mol/L NO3−N, 1.56×10−4 mol/L, PO4−P and supplements of other elements like Mn, Fe, I, etc.), male and female gametophytes ofU. pinnatifida kept growing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. The empirical formulaG
m=G
o·3m was established to estimate the output of vegetative gametophytes. Vigorous vegetative gametophyte cells began to form reproductive
structures (oogonium and spermatangium, when the temperature was lower than 25°C and other environmental factors were kept
optimal. The sufficient supply of gametophyte cells provided enough seeds for raisingUndaria sporelings on production scale. Controlled cross-breeding experiments using selected male and female gametophyte clones which
increase their cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes from the cross-breeding
had almost the same length and width increase rates as those of the control. The fact that vegetative gametophytes can be
purposely selected, propagated quickly, cross-bred, with the resulting sporophytes having almost the same characteristics
leads to a new way to select desiredUndaria strains for long time use without losing the desired economic characteristics.
Contribution No. 2696 from the Institute of Oceanology, Chinese Academy of Sciences.
Supported by National Natural Science Foundation of China. 相似文献
13.
The International Eq一lation of State of Seawater,1980 and the PraeticalSalinity Scale,1978 have been adoPted by theUNESCO江CES沼COR八APSOJoint Panel ono‘eanogral,hie Tables and Standards(JPOTS),and endorsed bythese organizati6ns(Miller‘〕and Poisson,1981;Uneseo,1981).Th已new equa-tion and the Praetieal Salinity Seale are to be used for all values Published fromJan .1,1982 .The new equation 15 aeeurate for use in all oeeanie surfaee waters,but eannot be aeeurately aPPlied to… 相似文献
14.
XIE Zhihao） ） XIAO Hui） TANG Xuexi） CAI Hengjiang） ） Marine Ecology Laboratory Ocean University of China Qingdao P. R. China ） Faculty of Life Science Biotechnology Ningbo University Ningbo P. R. China 《中国海洋大学学报(英文版)》2009,8(2)
The interspecific interactions between the rotifer Brachionus plicatilis and two harmful algal blooms(HAB) species were investigated experimentally by single culture method. B. plicatilis population and the growth of the two algae were compared at different algal cell densities. The results demonstrated that the B. plicatilis obtained sufficient nutrition from Prorocentrum donghaiense to support net population increase. With exposure to 2.5×104 cells mL-1 of P. donghaiense,the number of B. plicatilis increa... 相似文献
15.
From Oct., 1999 to Oct., 2000, the heterotrophic bacterial flora in the aquaculture area around Xuejiadao was investigated.
The result shows that the populations of the heterotrophic bacteria are heavier in summer and autumn than those in winter
and spring. The average populations in seawater, sediment, the surface of seaweed and the surface of fish are 1.4×104cfu mL−1, 5.4×106cfu g−1, 1.5×106cfu g−1 and 1.8×103cfu cm−2, respectively. A total of 301 strains were isolated, among them 259 were Gram-negative. All the Gram-negative bacteria belong
to 13 genera and some genera of Enterobacteriaceae. The communities of bacteria are slightly different among the samples. In the body surface of fish, Genus vibrio is dominant. In the remaining samples, dominant genus is Aeromonas. 相似文献
16.
Effects of Fe and Mn on the growth of a red tide dinoflagellateScrippsiella trochoidea (Stein) Loeblch III 总被引：1，自引：0，他引：1
Batch culture experiments were conducted with a red tide dinoflagellateScrippsiella trochoidea (Stein) Loeblch III collected from Jiaozhou Bay, Shangdong, China. Growth rates and oellular Chl—a were measured in media
with iron and manganese ion concentrations controlled at different levels using EdTA-trace metal buffer systems. Cell density
increased 3.2 times to 6.5 times over the range of lowest (0 mol/L) to highest (10−5 mol/L) iron and manganese ion concentrations. The range of cell density response was much lower than the range of total available
iron and manganese, which was >100—fold that of Fe. This nonlinear relationship indicates that the cells adapt to make more
efficient use of iron and manganese under limiting conditions. The cellular Chl—a content maximized after 3 days incubation
and then decreased gradually under either iron or manganese limitation conditions. It indicated that the algae gained higher
photosynthesis ability when transferred to a new environment. Growth responses to iron and manganese limitation can be both
modeled according to the equation of Monod. The half—saturation constant for growth,k, is 4.6×10−8 mol/L for Fe and 5.1×10−8 mol/L for Mn. Our results showed that the iron availability in Jiaozhou Bay does not limit the growth ofS. trochoidea.
Contribution No. 2831 from the Institute of Oceanology, Chinese Academy of Sciences.
Project 9389008 supported by NSFC; Study supported by PDB6. 相似文献
17.
We investigated the allelopathic properties of Alexandrium tamarense (Laboar) Balech on the growth of Prorocentrum donghaiense Lu, Chattonella marina (Subrahmanyan) Hara et Chihara and Heterosigma akashiwo (Hada) Hada in a laboratory experiment. We examined the growth of A. tamarense, C. marina, P. donghaiense and H. Akashiwo in co-cultures and the effect of filtrates from A. tamarense cultures in various growth phases, on the three harmful algal bloom (HAB)-forming algae. In co-cultures with A. tamarense, both C. marina and H. akashiwo were dramatically suppressed at high cell densities; in contrast, the growth of P. donghaiense varied in different inoculative ratios of A. tamarense and P. donghaiense. When the ratio was 1:1 (P. donghaiense: A. tamarense), growth of P. donghaiense was inhibited considerably, while the growth of P. donghaiense was almost the same as that of the control when the ratio was 9:1. The growth difference of P. donghaiense, C. marina and H. akashiwo when co-cultured with A. tamarense indicated that the allelopathic effect may be one of the important factors in algal competition and phytoplankton succession
involving A. tamarense. In addition, the filtrate from A. tamarense culture had negative impacts on these three HAB algae, and such inhibition varied with different growth phases of A. tamarense in parallel with reported values of PSP toxin content in Alexandrium cells. This implied that PSP toxin was possibly involved in allelopathy of A. tamarense. However, the rapid decomposition and inactivation of PSP toxin above pH 7 weakened this possibility. Further studies on
the allelochemicals responsible for the allelopathy of A. tamarense need to be carried out in future. 相似文献
18.
Effects of algal concentration and initial density on the population growth of Diaphanosoma celebensis Stingelin （Crustacea, Cladocera） 总被引：1，自引：0，他引：1
The effects of algal concentration and initial density on the population growth of the estuarine cladocera,Diaphanosoma celebensis Stingelin,were evaluated in an indoor experiment.A 2 × 4 layout that included two algal concentrations(Chlorella pyrenoidosa,1 × 106 and 3 × 106 cell/mL) and four inoculation densities(100,200,300 and 400 ind./L) were established.Diaphanosoma celebensis were reared in 150 mL flasks containing 50 mL of algal medium at 22°C,under salinity of 10 and a photoperiod of 12 h L:12 h D.T... 相似文献
19.
The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL−1) on scallop (Chlamy farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial
activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake,
phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL−1 B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear
time- and concentration-response to B[α]P. Bacteriolytic activity in 0.1μgL−1 B[α]P treatment group and antibacterial activity in 0.1 μgL−1 and 0.5 μgL- B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following
that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were
significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 μgL−1 B[α]P treatment group (P>0.05). Thus, B[α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution
and immunomodulation in aquatic organisms. 相似文献
20.
The ultrastructure of hemocytes ofPenaeus vannamei was examined by transmission electron microscope. Three types of hemocytes were identified: hyaline cell, small-granule cell
and large-granule cell. Hyaline cells are the smallest of the hemocytes, lack electron-dense granules, have average size of
11.11 μm (SE±0.50,n=10)×6.96μm (SE±0.55,n=10), comprise 25.8% (SE±2.87,n=4×10) of the hemocytes; small granule cells are the most abundant type of hemocytes, comprise 58.1% (SE±3.40,n=4×10) of the hemocytes, contain small electron—dense granules, have average size of 10.78 μm (SE±0.60,n=10)×8.63 μm (SE±0.44,n=10); and large-granule cells comprise 16.1% (SE±2.55,n=4×10) of the hemocytes, are filled with large electron dense granules, have average size of 12.51 μm (SE±0.63,n=10)×8.99μm (SE±0.71,n=10).
This research was supported by the National Climbing Project B, PDB6-6-3.
Contribution No. 3448 from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献