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1.
Copper is more toxic to all life stages of the copepod Tisbe than cadmium. The most sensitive life stage of Tisbe to both copper and cadmium is the one-day-old nauplius. The resistance of larval stages of Tisbe increases with age (one-day-old nauplii 48h lc50=0.3142 mg Cu l?1. and 0.5384 mg Cd l?1, 0.3415 mg Cu l?1. and 0.645 mg Cd l?1. for five-days-old nauplii and 0.5289 mg Cu l?1. and 0.9061 mg Cd l?1. for ten-days-old nauplii. The two reproductive stages of Tisbe tested (females with ovigerous bands and females bearing the first ovigerous sac) demonstrated an increased sensitivity to metals and proved more sensitive than the ten-days-old copepodids (only females with ovigerous bands had a similar sensitivity to copper with the ten-days-old copepodids).  相似文献   

2.
A static renewal toxicity test was conducted to evaluate the response of the copepod Acartia tonsa to bis(tributyltin) oxide (TBTO), the active compound of recently developed antifouling paints. Copepods were individually placed in test tubes containing 4 ml of 0.3, 0.5, 1.0, 1.7 or 3.0 μg l?1 TBTO with acetone and seawater. Thirty copepods were used for each treatment and control condition and all solutions were renewed daily. The 96-h LC50 was 1.0 μg l?1 with a 95% confidence interval between 0.8 and 1.2 μg l?1 TBTO. The 144-h EC50 was 0.4 μg l?1 TBTO which approaches concentrations recently measured in San Diego Bay. Some copepods exposed to 0.3 μg l?1 TBTO became moribund after six days.  相似文献   

3.
The effects of short-term exposure to tetravalent platinum on survival, opercular movement and post-treatment growth of coho salmon fry (Oncorhynchus kisutch) was investigated. Employing a static water acute toxicity bioassay with platinum as PtCl42HCl·6 H2O, at 8.5±0.2°C, and a water hardness of 55.9±3.5 mg l.?1 (as CaCo3), the 24, 48, and 96-h LC50 values were 15.5, 5.2, and 2.5 mg Pt4+ l.?1 respectively.Rates of opercular movement for fish exposed to platinum increased with increasing concentrations to a level of 1.0 mg l.?1. No further significant increases were evident above this level. Hypoactivity of fish exposed to 0.3 mg l.?1 and higher was evident during the acute toxicity bioassay and much of the post-treatment study. Post-treatment rate of growth for fish exposed to sublethal concentration of platinum for 96 h was less than that of the controls.All organs examined histopathologically were within normal ranges with the exception of the gills and olfactory organ. Lesions in fish exposed to concentration of 0.3 mg l.?1 and higher were characterized by branchial epithelial hypertrophy and hyperplasia, and necrosis of olfactory epithelial cells.  相似文献   

4.
《Marine pollution bulletin》2009,58(6-12):365-372
Since it is often difficult to estimate possible adverse effects due to contamination in selected ecosystems, multi-species biomonitoring may provide more information, taking into account different routes of exposure, ecological roles and metabolic capabilities of animals. In this context, we exposed for 7 days the mussel Mytilus galloprovincialis and the crab Carcinus aestuarii to 4-nonylphenol (NP), a well-known xenoestrogen. In mussels (0–0.2 mg NP l−1), we measured NP bioaccumulation in soft tissues and vitellogenin (Vg)-like protein levels in digestive glands from both males and females by the alkali-labile phosphate assay (ALP). As no reference data were available for crab exposure, the NP 96-h LC50 value was previously determined. Then, in sublethally exposed (0–1.0 mg NP l−1) male crabs, NP bioaccumulation and Vg levels were measured in hemolymph, gonads and digestive gland. Bioaccumulation of NP increased from 43 to 371 μg g−1 d.w. in mussels, and from 3.6 to 37 μg g−1 d.w. in crabs, depending on the NP concentration in water. Dose-dependent Vg-like protein induction was observed in both species, appearing to be related to NP bioaccumulation, although a partial decrease was recorded at the highest concentration tested. A similar trend was observed in both digestive gland and gonad of exposed crabs; Vg increased to a lesser extent, although significantly, in hemolymph. Results demonstrated that NP induces Vg synthesis both in male and female mussels, as well as in male crabs. On the basis of the responsiveness of both species investigated, a multi-species approach is indicated in biomonitoring programmes.  相似文献   

5.
Effects of silt loading on turbulence and sand transport   总被引:2,自引:1,他引:1  
The transport of bed material and fluid turbulence are affected by many factors,including the fine sediment load being carried in a channel.Current research has focused on sand-sized particles introduced to gravel beds,while the effect of silt load on sand transport has received less attention. Experiments on the effects of silt load,in concentrations 0-26,900 mg l-1,on sand transport were performed with a recirculating laboratory flume using three different sand bed configurations:ripples (Fr=0.24),dunes(Fr=0.34),and dunes(Fr=0.48).Three Acoustic Doppler Velocimeters were arranged to measure flow and turbulence quantities simultaneously in one vertical.Sand transport did not change in a consistent manner with increasing silt load,increasing up to 4,000 mg l-1 for dunes (Fr=0.48) and up to 2,000 mg l-1 for dunes(Fr=0.34) and then declining to near the clear water case with increasing silt concentrations.Silt addition for the ripple case caused a relatively small change in sand transport,decreasing with added silt up to approximately 2,000 mg l-1 and then increasing as silt went up to approximately 10,000 mg l-1.Dunes(Fr=0.48) decreased in length and height as silt increased,while dunes(Fr=0.34) did not show a consistent trend.A clear trend of decreasing Reynolds stress with increasing silt concentration was observed in the ripple case,with a 33% reduction in near-bed Reynolds stress caused by an 8,900 mg l-1 concentration of silt.  相似文献   

6.
Sea catfish (Arius felis) were exposed to aqueous solutions of reagent grade cupric chloride in artificial seawater (30.0±2.0‰, 21–23°C) in four static bioassays. The 24, 48, 72 and 96 h LC50 were calculated and found to be 5.43, 4.17, 3.57 and 2.40 mg l.?1 copper, respectively. Experimental concentrations of copper producing subtle behavioral changes in this species correspond to less than 0.3% of the 72 h LC50. Based on this comparison with literature values, a new, maximum ‘safe’ concentration for copper in marine waters of 0.01 mg l.?1 is proposed.  相似文献   

7.
The effect of hexavalent chromium on the carp,Cyprinus carpio was assessed using static bioassay. The 96h LC50 was found to be 93.6mg · l–1. In carp exposed to sublethal concentrations of chromium (15 and 25 mg · l–1), significant depletion of liver and muscle glycogen and decreasing leucocyte counts were recorded during prolonged exposure and with increasing concentration of chromium. The other blood parameters examined (haemoglobin, erythrocyte count, hematocrit, serum protein, serum glucose) increased similarly during exposure to two chromium concentrations.  相似文献   

8.
V. Chaplot  O. Ribolzi 《水文研究》2014,28(21):5354-5366
Dissolved organic carbon (DOC) is a key component of the global carbon cycle, but, to date, large uncertainties still exist on its source and fate in first‐order streams. In a 23 ha rangeland and steep‐slope headwater of South Africa, our aim was to quantify the contribution of overland flow (OF), soil water (SW) and ground water (GW) to DOC fluxes (DOCF), and to interpret the results in terms of DOC sources and fate. The average 2010–2011 DOC concentration (DOCC) at the catchment outlet was 4.7 mg C l?1 with a standard error of ±2.5 mg C l?1, which was significantly lower than in SW (15.2 ± 1.6 mg C l?1) and OF (11.9 ± 0.8 mg C l?1), but higher than in GW (2.3 ± 0.6 mg C l?1). Based on end‐member mixing using Si and Na concentration in the water compartments, the average SW contribution to DOCF was 66.4%, followed by OF (30.0%) and GW (3.6%). The resulting estimated DOCF at the catchment outlet was 8.05 g C m2 y?1. This was much higher than the observed value of 2.80 g C m2 y?1, meaning that 5.25 g C m2 y?1 or 65% of the DOC is lost during its downslope and/or downstream transport to the catchment outlet. Complementary investigations revealed that the DOCC in SW dropped from 15.2 ± 1.6 to 2.6 ± 0.3 mg C l?1 during its downslope transport to the river system, which corresponded to a net loss of 5.10 g C m2 y?1, or 97% of the catchment DOC losses. These results on DOC sources and potential fate in headwaters are expected to improve our understanding of the impact of hydrology on the global C‐cycle. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

9.
The accumulation of crimidin in total samples and in some organs of five fish species was studied under experimental conditions. The species were Cyprinus carpio, Carassius carassius, Tinca tinca, Scardinus erythrophthalmus and Leucaspius delineatus. Fish were exposed to concentrations of 10 mg · l?l and 50 mg · l?1. During short-term experiments water and fish samples were taken at intervals of t = 0, 6, 12, 24, 48 and 72 hours, during the long-term experiment sampling was performed weekly for six weeks. Total samples were analysed for C. carassius, T. tinca, S. erythrophthalmus and L. delineatus. Samples of individual organs and tissues were taken as follows: C. carpio – gills, digestive tract, muscular tissue, kidneys, gonads; C. carassius – gills, digestive tract, muscular tissue, ovaries, testes. Crimidin was determined by gas chromatography. In samples from the short-term experiments at a concentration of 10 mg · l?1 in the water the amount was roughly 101–10 mg · kg?1, at a concentration of 50 mg · l?1 in the water roughly 101 mg · kg?1. In most cases the accumulation coefficient was lower than 1.0. The accumulation capacity of individual fish species did not differ greatly. Of the internal organs only the kidneys had a high accumulation capacity, otherwise the highest values were found in muscular tissue and the gills. After one – three weeks the amount of crimidin in most organs falls, and after transfer to clean water there is a general sharp decline. Thus crimidin is not firmly bound in the body.  相似文献   

10.
The weight-specific respiration rate (μl O2 mg−1 AFDW h−1) of three species of leech from Lake Esrom, Denmark, Glossiphonia concolor, G. complanata and Helobdella stagnalis was measured in a closed stirred chamber with a micro electrode. At declining oxygen concentration (mg O2 l−1) all three species expressed moderate ability to regulate respiration, in G. concolor and G. complanata down to 2 mg O2 l−1, in H. stagnalis down to 0.75 mg O2 l−1. Survival in anoxia was measured in closed bottles. The time to 50% survival (LD50) was 30 days in G. concolor at 20 °C and 30 and 4 days in H. stagnalis at 10 and 20 °C, respectively. The results were discussed in relation to habitat and spatial distribution of the three species in the lake.  相似文献   

11.
Laboratory reared veliger larvae of the common mussel Mytilus edulis were grown for 15 days in filtered seawater containing 10 μg l?1, 1 μg l?1 and 0.1 μg l?1 tributyltin oxide (TBTO), the biocide in recent antifouling paint formulations. No larvae survived longer than 5 days in 10 μg l?1 TBTO or 10 days in 1 μg l?1 TBTO. Approximately half the larvae subjected to 0.1 μg l?1 TBTO were dead by day 15 (approximate 15 day LC50 = 0.1 μg l?1 TBTO) and most of the surviving larvae were moribund and had grown significantly more slowly than controls. It is noted that current recorded levels of TBT at several UK estuarine sites are higher than 0.1 μg l?1 and there has therefore probably been a high mortality of mussel larvae at such sites. The results are discussed in relation to the reported short-term toxicity of organotins to other marine organisms and the value of carrying out longer-term tests at low levels of TBT is pointed out.  相似文献   

12.
Pre-exposure of marine mussels to low levels of mercury (0.5 μg l?1 and less) enhanced their tolerance to more toxic levels. Seawater mercury concentrations similar to our experimental pre-exposure levels have been reported in mercury-contaminated areas where mussels presently exist. A higher pre-exposure concentration (5 μg l?1) was not effective in inducing enhanced mercury tolerance. The relative effectiveness of pre-exposure concentration in inducing enhanced tolerance was related to induction of mercury-binding proteins, the degree of saturation of mercury on the proteins, and the extent of binding of mercury to other subcellular fractions.  相似文献   

13.
Sea mullet (Mugil cephalus) were exposed to aqueous suspensions of tech-DDT in seawater, salinity 29.5±0.5‰. The 48 h LC50 calculated from these static bioassays ranged from 0.042 to 0.056 mg l.?1 DDT. Median survival times were then estimated at 15, 20, 25 and 30°C using the upper level of the 48 h LC50 range of concentrations, i.e. 0.056 mg l.?1. Results indicated that an increase in temperature produced a decrease in toxicity. However, 25°C was the only temperature at which the increase in survival time was statistically significant. Size differences within experimental fish did not contribute to MST.  相似文献   

14.
Since it is often difficult to estimate possible adverse effects due to contamination in selected ecosystems, multi-species biomonitoring may provide more information, taking into account different routes of exposure, ecological roles and metabolic capabilities of animals. In this context, we exposed for 7 days the mussel Mytilus galloprovincialis and the crab Carcinus aestuarii to 4-nonylphenol (NP), a well-known xenoestrogen. In mussels (0–0.2 mg NP l−1), we measured NP bioaccumulation in soft tissues and vitellogenin (Vg)-like protein levels in digestive glands from both males and females by the alkali-labile phosphate assay (ALP). As no reference data were available for crab exposure, the NP 96-h LC50 value was previously determined. Then, in sublethally exposed (0–1.0 mg NP l−1) male crabs, NP bioaccumulation and Vg levels were measured in hemolymph, gonads and digestive gland. Bioaccumulation of NP increased from 43 to 371 μg g−1 d.w. in mussels, and from 3.6 to 37 μg g−1 d.w. in crabs, depending on the NP concentration in water. Dose-dependent Vg-like protein induction was observed in both species, appearing to be related to NP bioaccumulation, although a partial decrease was recorded at the highest concentration tested. A similar trend was observed in both digestive gland and gonad of exposed crabs; Vg increased to a lesser extent, although significantly, in hemolymph. Results demonstrated that NP induces Vg synthesis both in male and female mussels, as well as in male crabs. On the basis of the responsiveness of both species investigated, a multi-species approach is indicated in biomonitoring programmes.  相似文献   

15.
《Marine pollution bulletin》2012,64(5-12):201-208
Flow cytometry was used to examine immune responses in haemocytes of the green-lipped mussel Perna viridis under six combinations of oxygen level (1.5 mg O2 l−1, 6.0 mg O2 l−1) and temperature (20 °C, 25 °C and 30 °C) at 24 h, 48 h, 96 h and 168 h. The mussels were then transferred to normoxic condition (6.0 mg O2 l−1) at 20 °C for further 24 h to study their recovery from the combined hypoxic and temperature stress. Esterase (Est), reactive oxygen species (ROS), lysosome content (Lyso) and phagocytosis (Pha) were reduced at high temperatures, whereas hypoxia resulted in higher haemocyte mortality (HM) and reduced phagocytosis. For HM and Pha, changes were observed after being exposed to the stresses for 96 h, whereas only a 24 h period was required for ROS and Lyso, and a 48 h one for Est. Recovery from the stresses was observed for HM and Pha but not other immune responses.  相似文献   

16.
Solute concentrations and fluxes in rainfall, throughfall and stemflow in two forest types, and stream flow in a 90 ha catchment in southern Chile (39°44′S, 73°10′W) were measured. Bulk precipitation pH was 6·1 and conductivity was low. Cation concentrations in rainfall were low (0·58 mg Ca2+ l?1, 0·13 mg K+ l?1, 0·11 mg Mg2+ l?1 and <0·08 mg NH4–N l?1), except for sodium (1·10 mg l?1). Unexpected high levels of nitrate deposition in rainfall (mean concentration 0·38 mg NO3–N l?1, total flux 6·3 kg NO3–N ha?1) were measured. Concentrations of soluble phosphorous in bulk precipitation and stream flow were below detection limits (<0·09 mg l?1) for all events. Stream‐flow pH was 6·3 and conductivity was 28·3 μs. Stream‐water chemistry was also dominated by sodium (2·70 mg l?1) followed by Ca, Mg and K (1·31, 0·70 and 0·36 mg l?1). The solute budget indicated a net loss of 3·8 kg Na+ ha?1 year?1, 5·4 kg Mg2+ ha?1 year?1, 1·5 kg Ca2+ ha?1 year?1 and 0·9 kg K+ ha?1 year?1, while 4·9 kg NO3–N ha?1 year?1 was retained by the ecosystem. Stream water is not suitable for domestic use owing to high manganese and, especially, iron concentrations. Throughfall and stemflow chemistry at a pine stand (Pinus radiata D. Don) and a native forest site (Siempreverde type), both located within the catchment, were compared. Nitrate fluxes within both forest sites were similar (1·3 kg NO3–N ha?1 year?1 as throughfall). Cation fluxes in net rainfall (throughfall plus stemflow) at the pine stand generally were higher (34·8 kg Na+ ha?1 year?1, 21·5 kg K+ ha?1 year?1, 5·1 kg Mg2+ ha?1 year?1) compared with the secondary native forest site (24·7 kg Na+ ha?1 year?1, 18·9 kg K+ ha?1 year?1 and 4·4 kg Mg2+ ha?1 year?1). However, calcium deposition beneath the native forest stand was higher (15·9 kg Ca2+ ha?1 year?1) compared with the pine stand (12·6 kg Ca2+ ha?1 year?1). Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

17.
The effect of patchy colonies of the invasive zebra mussel (Dreissena polymorpha) on sedimentary processes was investigated in a mesotrophic lake (Plateliai Lake, Lithuania). Benthic fluxes of O2, TCO2, CH4, Mn2+, Fe2+, N2, the inorganic forms of N, Si and P and dissolved organic C and N were quantified by dark incubations of sediments cores, with and without D. polymorpha. Individual mussels also were incubated for metabolic measurements. Sediments with D. polymorpha had significantly higher O2 and TCO2 fluxes and displayed higher rates of denitrification. The presence of mussels also resulted in higher regeneration of P and N (mostly as ammonium) while the effect on Si was not significant. However, likely due to the low zebra mussel biomass (57.2 ± 25.3 gSFDW m?2), biodeposition has not changed the ratio between anaerobic and total respiration. Methane and reduced metals fluxes were in fact similar in the presence and absence of D. polymorpha. Incubations of mussels without sediments confirmed that bivalve metabolism was the main driver of benthic respiration and nutrient recycling. Nitrate production suggested the presence of nitrifiers associated with the molluscs. The main outcome of this study was that zebra mussels alter the quantity and the stoichiometry of nutrients regenerated by the benthic compartment. The enhancement of nitrogen loss via denitrification, by a factor of 1.5, was much less pronounced than the increase in ammonium recycling rate, stimulated by a factor of 33. Negligible PO4 3? fluxes in bare sediments (?3.4 ± 6.8 μmol m?2 h?1) increased in the presence of mussels and considerable amounts of this nutrient (69.6 ± 29.4 μmol m?2 h?1) were mobilized to the water column. Further research should address other nutrient sources to the lake to verify whether altered rates and stoichiometry of benthic regeneration can affect primary producer community composition and activity.  相似文献   

18.
Surface waters associated with peatlands, supersaturated with CO2 and CH4 with respect to the atmosphere, act as important pathways linking a large and potentially unstable global repository of C to the atmosphere. Understanding the drivers and mechanisms which control C release from peatland systems to the atmosphere will contribute to better management and modelling of terrestrial C pools. We used non‐dispersive infra‐red (NDIR) CO2 sensors to continuously measure gas concentrations in a beaver pond at Mer Bleue peatland (Canada); measurements were made between July and August 2007. Concentrations of CO2 in the surface water (10 cm) reached 13 mg C l?1 (epCO2 72), and 26 mg C l?1 (epCO2 133) at depth (60 cm). The study also showed large diurnal fluctuations in dissolved CO2 which ranged in amplitude from ~1·6 mg C l?1 at 10 cm to ~0·2 mg C l?1 at 60 cm depth. CH4 concentration and supersaturation (epCH4) measured using headspace analysis averaged 1·47 mg C l?1 and 3252, respectively; diurnal cycling was also evident in CH4 concentrations. Mean estimated evasion rates of CO2 and CH4 over the summer period were 44·92 ± 7·86 and 0·44 ± 0·25 µg C m?2s?1, respectively. Open water at Mer Bleue is a significant summer hotspot for greenhouse gas emissions within the catchment. Our results suggest that CO2 concentrations during the summer in beaver ponds at Mer Bleue are strongly influenced by biological processes within the water column involving aquatic plants and algae (in situ photosynthesis and respiration). In terms of carbon cycling, soil‐stream connectivity at this time of year is therefore relatively weak. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   

19.
The effects of oxygen supply rate and the presence or absence of nutrients on the kinetics of phenol degradation and oxygen consumption by a mixed microbial population were tested in a model system. The values for the maximum specific rate of phenol degradation (qSmax), the saturation constant (KS), and the inhibition concentration (SCR) were determined for unlimited growth (KLa = 340 h?1, growth medium) with 1.7 mmol g?1 h?1, 65 mg L?1, and 190 mg L?1. Under limitation conditions, alterations occur depending on the type of limitation. Nutrient limitations lead to values of 0.8 mmol g?1 h?1, 45 mg L?1, and 160 mg L?1, and oxygen limitations lead to 1.2 mmol g?1 h?1. 30 mg L?1, and 120 mg L?1, respectively. The results suggest that with excess oxygen, the rate of phenol degradation was higher and the inhibition effect of phenol was suppressed to some extent. Under the same high oxygen supply rate, the presence of nutrients in the model water significantly supported the phenol degradation rate.  相似文献   

20.
The effects of Cu, Cd, Zn and Cr on the survival and feeding behaviour of the sandy shore scavenging gastropod Nassarius festivus were compared. The 96-h LC50 for Cu, Cd, Zn and Cr were 0.36, 1.52, 1.76 and 36.9 mg l−1, respectively. Four sublethal concentrations of each metal plus a control were prepared and the snails were exposed to experimental solutions for 96 h. Feeding behaviour was studied after the snails were starved for five days. As compared with the control, the number of individuals feeding was significantly reduced by exposure to 0.05 mg l−1 Cu, 0.2 mg l−1 Zn, 0.5 mg l−1 Cd and 5 mg l−1 Cr. The time spent feeding was greater for individuals exposed to greater concentrations of Zn and Cd but no effect was found for Cu and Cr. Chemoreception of food was studied by placing the snails at a fixed distance of 15 cm from the bait. The success rate of reaching the bait was less for individuals exposed to Cr but no effect was found for Zn, Cu or Cd. The time required for an individual to reach the bait decreased as the concentration of Zn increased. In contrast, a longer time was required for individuals exposed to Cr whereas the effect of Cd and Cu was insignificant. The potential of using feeding behaviour and chemoreception in contaminant evaluations is discussed.  相似文献   

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