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1.
107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases. 相似文献
2.
ZHU Luying ZHANG Xuecheng REN Xueying ZHU Qinghua 《中国海洋大学学报(英文版)》2008,7(1):83-88
The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and docosahexaenoic acid （C22：6 n-3, DHA） production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23 ℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. limacinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture conditions, the maximum DHA yield achieved in flasks was 4.08 g L^-1 after 5 d of cultivation 相似文献
3.
YUE Lixi MA Chunling and CHI Zhenming* UNESCO Chinese Center of Marine Biotechnology Ocean University of China Qingdao P.R.China 《中国海洋大学学报(英文版)》2006,5(4)
1IntroductionWater co-produced with oil and gas recovery istermed oil-field produced water.This water is consid-ered to be of the largest volume in the waste streamfromoil and gas production processes.The volume ofproduced water can be more than10ti mes t… 相似文献
4.
Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter. 相似文献
5.
Extracellular enzymatic activities of cold-adapted bacteria from polar oceans and effect of temperature and salinity on cell growth 总被引：1，自引：0，他引：1
The potential of 324 bacteria isolated from different habitats in polar oceans to produce a variety of extracellular enzymatic activities at low temperature was investigated. By plate assay, lipase, protease, amylase, gelatinase, agarase, chitinase or cellulase were detected. Lipases were generally present by bacteria living in polar oceans. Protease-producing bacteria held the second highest proportion in culturable isolates. Strains producing amylase kept a relative stable proportion of around 30% in different polar marine habitats. All 50 Arctic sea-ice bacteria producing proteases were cold-adapted strains, however, only 20% were psychrophilic. 98% of them could grow at 3% NaCl, and 56% could grow without NaCl. On the other hand, 98% of these sea-ice bacteria produced extracellular proteases with optimum temperature at or higher than 35℃, well above the upper temperature limit of cell growth. Extracellular enzymes including amylase, agarase, cellulase and lipase released by bacteria from seawater or sediment in polar oceans, most expressed maximum activities between 25 and 35℃. Among extracellular enzymes released by bacterial strain BSw20308, protease expressed maximum activity at 40℃, higher than 35℃ of polysaccharide hydrolases and 25℃ of lipase. 相似文献
6.
Dewi Seswita Zilda 《中国海洋大学学报(英文版)》2012,11(2):213-218
A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia’s hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability. 相似文献
7.
Harmful algal blooms (HABs) have led to extensive ecological and environmental issues and huge economic losses. Various HAB control techniques have been developed, and biological methods have been paid more attention. Algicidal bacteria is a general designation for bacteria which inhibit algal growth in a direct or indirect manner, and kill or damage the algal cells. A metabolite which is strongly toxic to the dinoflagellate Alexandrium tamarense was produced by strain DH46 of the alga-lysing bacterium Alteromonas sp. The culture conditions were optimized using a single-factor test method. Factors including carbon source, nitrogen source, temperature, initial pH value, rotational speed and salinity were studied. The results showed that the cultivation of the bacteria at 28°C and 180 r min?1 with initial pH 7 and 30 salt contcentration favored both the cell growth and the lysing effect of strain DH46. The optimal medium composition for strain DH46 was determined by means of uniform design experimentation, and the most important components influencing the cell density were tryptone, yeast extract, soluble starch, NaNO3 and MgSO4. When the following culture medium was used (tryptone 14.0g, yeast extract 1.63g, soluble starch 5.0 g, NaNO3 1.6 g, MgSO4 2.3 g in 1L), the largest bacterial dry weight (7.36 g L?1) was obtained, which was an enhancement of 107% compared to the initial medium; and the algal lysis rate was as high as 98.4% which increased nearly 10% after optimization. 相似文献
8.
The marine yeast strain W6b isolated from sediment of the South China Sea was found to produce a cell-bound acid protease.
The crude acid protease produced by this marine yeast showed the highest activity at pH 3.5 and 40 °C. The optimal pH and
temperature for the crude acid protease were in agreement with those for acid protease produced by the terrestrial yeasts.
The optimal medium of the acid protease production was seawater containing 1.0% glucose, 1.5% casein, and 0.5% yeast extract,
and the optimal cultivation conditions of the acid protease production were pH 4.0, a temperature of 25 °C and a shaking speed
of 140 rmin−1. Under the optimal conditions, 72.5 UmL−1 of acid protease activity could be obtained in cell suspension within 48 h of fermentation at shake flask level. The acid
protease production was induced by high-molecular-weight nitrogen sources and repressed by low-molecular-weight nitrogen sources.
Skimmed-milk-clotting test showed that the crude acid protease from the cell suspension of the yeast W6b had high skimmed
milk coagulability. The acid protease produced by M. reukaufii W6b may have highly potential applications in cheese, food and fermentation industries. 相似文献
9.
A total of 400 yeast strains from seawater, sediments, saltern mud, marine fish guts, and marine algae were obtained. The
protease activity of the yeast cultures was estimated, after which four strains (HN3.11, N11b, YF04C and HN4.9) capable of
secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans, Yarrowia lipolytica, Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11, YF04C, and HN4.9 was 9.0, while that of the protease
produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11, N11b, and YF04C,
and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases, the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%)
and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant
activity and ACE-inhibitory activities. 相似文献
10.
LI Haifeng CHI Zhenming * WANG Xiaohong and MA Chunling UNESCO Chinese Center of Marine Biotechnology Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2007,6(1):60-65
1 Introduction Amylases are enzymes which hydrolyze starch molecules to give diverse products including dextrin and pro-gressively smaller polymers composed of glucose units(Windish et al., 1965; Pandey et al., 2000; Chi et al.2001). Amylase is a kind of very important enzyme andconstitutes a class of industrial enzymes sharing approximately 25% of the enzyme market (Sindhu et al.1997; Rao et al., 1998). Amylases are universally distributed throughout the animal, plant and microbial kingdoms… 相似文献
11.
尿素包合法富集蛇鲻鱼油中EPA和DHA的研究 总被引：2，自引：0，他引：2
以蛇鲻精制鱼油为原料,采用氢氧化钾乙醇皂化和硫酸酸化制备鱼油混合脂肪酸,以EPA和DHA含量为指标,探讨了尿素包合法富集鱼油中EPA和DHA的工艺条件。结果表明:以乙醇为溶剂,在尿脂质量比为2︰1和包合温度0℃的条件下进行一次性包合20 h后,富集产物中EPA和DHA总含量达到了69.74%,其中EPA含量为22.05%,DHA含量为47.69%,达到了保健鱼油的生产要求。 相似文献
12.
为研究生物酶采油解堵剂中产蛋白酶菌株的初、复筛选及培养条件优化,从大庆原油样品中筛选菌种,通过水解酪素的透明圈实验及福林酚测蛋白酶酶活的方法进行菌株的初、复筛选;以蛋白酶酶活为优化指标,采用单因素实验对筛选的产蛋白酶菌株的培养基及培养条件进行优化,优化最适培养基:可溶性淀粉为15g/L,蛋白胨为20g/L,酵母膏为20g/L,NaCl为1.0g/L,CaCl2为0.02g/L,Na2HPO4为0.2g/L,NaH2PO4为0.1g/L;在初始pH为6.0、接种量为5%(体积分数)、温度为31℃、摇床转速为160r/min的条件下,培养72h后,菌株的蛋白酶酶活为551.0U/mL,为复筛选菌株的蛋白酶酶活的22.92倍,即为菌株生长繁殖及代谢的最佳条件,能够获得更高的蛋白酶酶活,有利于后续实验的进行.结果表明:菌株产蛋白酶对原油作用效果为发酵液表面张力从作用前的56.2mN/m降低到作用后的30.5mN/m,表面张力显著降低,还有降解降黏原油等效果,具有一定的研究价值. 相似文献
13.
异育银鲫气单胞菌病原菌鉴定和药敏试验 总被引：1，自引：0，他引：1
从患病异育银鲫体内分离到5株可疑菌株,经API 20NE细菌生化鉴定,YCS07-03和YZ07-11株鉴定率为99.2%和99.3%。注射感染中5×108 CFU.mL-1剂量组表现出较强的致病性,12 d死亡率100%。菌株在营养肉汤培养液中呈均匀混浊状;在营养琼脂平皿中呈灰白色半透明菌落;在血琼脂培养基中呈光滑灰白色菌落,YZ07-11株出现透明的β溶血环;在TCBS培养基呈黄色菌落;在SS和麦康凯培养基中形成无色透明的扁平菌落。经负染电镜观察,菌株两端圆,呈直杆状,无芽孢,极生单鞭毛,菌株大小(短径×长径)分别为1.53μm×2.71μm和0.80μm×2.12μm。菌株经生化实验鉴定为温和气单胞菌(Aeromonas sobria)和嗜水气单胞菌(Aeromonas hydrophila)。试验结果表明:菌株对丁胺卡那霉素、头孢噻肟、庆大霉素、左氟沙星、复合磺胺、氟嗪酸、洛美沙星、复方新诺明、链霉素和新霉素均产生高度敏感;对万古霉素为中度敏感;氨苄青霉素、羧苄青霉素、强力霉素和青霉素产生耐药。 相似文献
14.
YUE Lixi MA Chunling CHI Zhenming 《中国海洋大学学报(英文版)》2006,5(4):333-338
Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment. 相似文献
15.
Abdul Hameed Shahida Hasnain 《中国海洋湖沼学报》2005,23(4):433-441
Many unicellular cyanobacteria were isolated from different places： fields, ponds, polluted water, and soils from Muredkey and Kasur tannery areas, near Lahore, Pakistan. Different media like BG 11 medium, Bold Basal medium, Chu＇s # 10 medium and Gotham＇s medium, in standard forms and with slight variations of ingredients, and different pH, temperature and light regimes were checked for the optimum growth of the isolates. The isolation pro- cedure was repeated with different concentrations of chromium to select the resistant strains. These selected strains grew on chromium of the range 100-200 μg/ml in BG 11 medium. Cyanobacteria were maintained in solid and liquid media with/without shaking. Cyanobacterial strains were collected from natural habitats that were accompanied by a diversified group of organisms including bacteria, protozoan, and rotifers etc. In order to eliminate these agents termed as contaminants, we used several methods including phenol treatment, use of antibiotic and careful manual picking of unicellular cyanobacteria. Resistance of these strains against different heavy metals （ZnSO4, MnSO4, NiSO4, COCl2, Pb（NO3）3, CuSO4, HgCl2, AgNO3 and CdCl2） and antibiotics （erythromycin, streptomycin, kanamycin, chloramphenicol, neomycin） was evaluated. Optimum temperature was 30℃ with variable pH for the reduction of Cr^6＋ in to Cr^3＋ in majority of strains. 相似文献
16.
The marine yeast strain N13d, producing an extracellular amylase, was isolated from the deep sea sediments of the Pacific
Ocean. This strain was identified to be Aureobasidium pullulans by 18S rRNA gene sequence analysis and routine yeast identification methods. The optimal sea water medium for amylase production
by this yeast strain was 1.0% peptone and 1.0% soluble starch with pH 4.0. The optimal conditions for amylase production by
this yeast strain were with temperature 28 °C, aeration rate 6 Lmin−1 and agitation speed 250 rmin−1. Under these conditions, 58.5 units of amylase activity per mg protein were produced within 56 h of fermentation. 相似文献
17.
18.
酶解法提取鱼油的工艺参数优化 总被引：1，自引：0，他引：1
利用蛋白酶酶解法从黄鳍金枪鱼(Thunnus albacares)加工的下脚料———鱼头中提取鱼油。以鱼油提取率和感官特征为指标,通过正交优化实验设计,获得了胰蛋白酶提取鱼油的最佳酶解工艺参数:酶解温度45℃,酶添加量1.5%,料液质量比1∶1,酶解时间4 h,酶解pH 8。在该条件下,鱼油的提取率为4.34%,理化指标除过氧化值外均达到SC/T3502-2000的粗鱼油二级标准,多不饱和脂肪酸总含量高达38.47%,其中DHA和EPA的含量分别为23.63%和4.84%。 相似文献
19.
INTRODUCTIONMarinemicroalgaeacquiretheirinorganiccarbonforphotosynthesisfromseawaterwhereinorganiccarbonmaybepresentasfreeCO2 ,H2 CO3andHCO-3 andCO2 -3 .TheproportionsofdifferentDICformsareprimarilyrelatedtopH ,andtotemperatureandsalinitytoalessextent.ThetotalDICisashighas2mmol Linseawa terbutthisismainlyintheformofHCO-3 .InnormalpHrangeof7.8-8.2inseawater,HCO-3 accountsfororethan 90 %ofthetotalDIC,whilefreeCO2 islessthan1 % .TheequilibriumconcentrationoffreeCO2 isonly1 2 μm… 相似文献
20.
A Gram negative bacterium Ar/W/b/75°25＇N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25＇N, and longitude 162°25＇W in Chukchi sea, Arctic. The strain can grow at the temperature range from 7℃ to 30℃, and grow better at 30(℃. It can not grow at 40℃. Keeping certain salinity concentration in medium is necessary for cell growth. It grows well in medium containing salinity concentration from 0. 5 % to 10 % sodium chloride. Glucose, sucrose and soluble starch can be utilized by the strain, among which glucose is the optimal carbon source. Peptone is the optimal organic nitrogen source for cell growth and protease producing, and ammonium nitrate is the optimal inorganic nitrogen source.About 75.7% of total protease of the strain are extracellular enzyme. Optimal temperature for proteolytic activity is at 40℃. Protease of the strain keeps stable below 40℃, and shows high proteolytic activity within the pH range from 7 to 11. 相似文献