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1.
The aim of this study was to characterize both the viable airborne bacteria and fungi collected in different types of wastewater treatment plants in Poland. Bacterial and fungal aerosols in five different wastewater treatment plants in Poland were studied, including the pioneering antibiotic resistance testing of airborne bacteria. Concentration level was measured using the six-stage Andersen impactor. After incubation, bioaerosol particles captured on nutrient media on Petri dishes were quantitatively evaluated and qualitatively identified. It was found that the concentration levels of both airborne bacteria and fungi ranged from 10² to 10³ colony-forming units/m³, in all stages of the wastewater treatment plants. The patterns of size distributions of airborne bacteria and fungi were very similar, showing dominant peaks typically in the size range between 3.3 and 4.7 μm. The majority fraction of small bacterial and fungal particles (less than 4.7 μm) in the studied areas indicates that the bioaerosol is relatively fresh, and mostly of local origin. The Gram-positive cocci and nonsporing Gram-positive rods were the dominating forms in the studied wastewater treatment plants environment. The existing differences in the concentration levels between sampling sites enabled identification of the main bioaerosol sources. The highest concentration of bacterial aerosol appeared in sections, where activated sludge post-processing and mechanical purifying are conducted. The most frequently occurring species in the sampled bacterial aerosol were Gram-positive cocci and nonsporing Gram-positive rods. Multi-antibiotic resistance testing showed that among the isolated airborne bacteria, the most antibiotic-resistant features were present among Bacillus species (especially Bacillus mycoides).  相似文献   

2.
The enzyme-labeled fluorescence (ELF-97®) substrate produces an insoluble, fluorescent yellow-green product at the site of alkaline-phosphatase (AP) activity. Fifteen coastal phytoplankton species were tested to determine if AP activity could be detected in phosphate-depleted media. All species tested, except Synechococcus bacillaris, expressed AP activity. Subsequently, threshold concentrations of soluble reactive phosphate (SRP) at which AP activity could be detected by ELF-97® were determined for Chaetoceros neogracile, Chlorella autotrophica, Isochrysis sp., Prorocentrum minimum, and Tetraselmis chui. Microalgal species differed significantly in the SRP concentration at which AP activity was first detectable (10.1–16.4 µM), well above concentrations normally considered limiting for phytoplankton. P. minimum began to express AP activity at a higher SRP concentration than the other algal species; this may be attributable to a relatively high DNA/cell ratio in P. minimum, compared to the other phytoplankton. Thus, phytoplankton species may respond to phosphorus deficiency at high SRP concentrations.  相似文献   

3.
Municipal solid waste poses a risk on surrounding environment and public health, mainly because of unscientific disposal and shortage of facilities for proper handling and recycling of leachate. This research article objective is to pinpoint the indigenous fungal isolates of waste leachate samples. Therefore, we carried out biosorption of Cd2+ tested the applicability by applying indigenous fungal isolates. The limited number of fungal isolates was found based on their ability for biosorption of Cd2+ metal. The fungal strains Trichoderma sp., Aspergillus niger and Aspergillus flavus were reported as potential strains for metal exclusion ability from the leachate. Among them, the Trichoderma sp. was found as excellent fungal agent for Cd2+ absorption. The optimum pH was 5.5 ± 1, temperature 45 °C, and spore concentration 10?5 to achieve the maximum biosorption, and 35 days of incubation period were required by three strains. The maximum metal biosorption achieved was comparable for the three isolates: 56.34% by Trichoderma sp., 44.74 and 42.04% by A. niger and A. flavus, respectively. Concluding, the further intending application to identified potentially fungal isolates is able to improve the efficiency of metal biosorption. These strains are recommended for development of consortia could become a best technique for MSW leachate treatment if its reliability and applicability should be verified prior to technology acceptance.  相似文献   

4.
Applying biodegradable polymers in membrane preparation for separation processes is expected to be helpful in solving waste disposal problem. In this study, biodegradable blend membranes of cellulose acetate (CA)/poly(butylene succinate) (PBS) were prepared and the microorganisms possessing the ability of degrading them were isolated from soil. The isolates were classified into two groups of bacteria and fungi. The membranes biodegradation after cultivation by the isolates in liquid media was further confirmed by weight loss measurement, Fourier transform infrared analysis and scanning electron microscopy. Findings revealed that CA degradation could be improved in the blend membrane structure. Moreover, strain BG106 and BG111 could degrade the membranes at the fastest rate among the other bacteria and fungi, respectively. To our knowledge, BG111 was found to belong to Alternaria sp. and this is the first report of its PBS degradation activity. Moreover, the PBS degradability of BG104 (B. Safensis), BG105 (Achromobacter insuavis) and BG106 (Brevibacterium halotolerans) strains was reported for the first time.  相似文献   

5.
The potential of the autoclaved Tunisian landfill leachate treatment using microalgae (Chlorella sp.) cultivation was investigated in this study. Landfill leachate was collected from Borj Chakir landfill, Tunisia. A full factorial experimental design 22 was proposed to study the effects of the incubation time and leachate ratio factors on the organic matter removal expressed in chemical oxygen demand (COD) and ammoniacal nitrogen (NH4─N) and on the biological response of Chlorella sp. expressed by the cell density and chlorophyll content. All experiments were batch runs at ambient temperature (25 ± 2 °C). The Chlorella sp. biomass and chlorophyll a concentrations of 1.2 and 5.32 mg L?1, respectively, were obtained with 10% leachate spike ratio. The obtained results showed that up to 90% of the ammoniacal nitrogen in landfill leachate was removed in 10% leachate ratio spiked medium with a residual concentration of 40 mg L?1. The maximum COD removal rate reached 60% within 13 days of incubation time indicating that microalgae consortium was quite effective for treating landfill leachate organic contaminants. Furthermore, with the 10% leachate ratio spiked medium, the maximum lipid productivity was 4.74 mg L?1 d?1. The present study provides valuable information for potential adaptation of microalgae culture and its contribution for the treatment of Tunisian landfill leachate.  相似文献   

6.
Mercury (Hg) concentrations in air, effluent water, landfill gas, leachate, groundwater, and soil at a hazardous solid waste landfill site in Korea were measured along with air–soil surface Hg exchange fluxes at the site. The concentrations and fluxes were considerably higher than have been found elsewhere in Korea. Gaseous Hg concentrations in the air peaked during the day, coinciding with Hg being released from the landfill surface. This suggests that air–soil exchange increased the Hg concentrations in the atmosphere. The air–soil exchange flux increased abruptly when solar radiation reached the soil surface. The Hg flux peaked about 3 h before the solar radiation peaked, possibly because reducible Hg was abundant at the soil surface. The Hg emission flux activation energy (E a) was low, indicating that the Hg species present and Hg–soil binding were probably not as important (because of the high Hg content of the soil) as in previous studies. The methylmercury to total Hg ratios in the discharged effluent, groundwater, and leachate was clearly higher than typically found in coastal water and freshwater, suggesting bacteria caused active methylation to occur under the reducing conditions in the anaerobic landfill. The results suggested that considerable amounts of Hg are probably transported from the landfill to nearby environmental media and that this will continue if waste with a high Hg content continues to be added to the landfill without being pretreated.  相似文献   

7.
Four bacterial strains, capable of degrading diesel oil, n-alkanes or hexadecane, were isolated from soils contaminated with petroleum oil and identified. Strains of Pseudomonas sp., Pseudomonas putida TPHK-1 and Pseudomonas aeruginosa TPHK-4, were more efficient in degrading high concentrations of the hydrocarbons than the other two strains, Stenotrophomonas maltophilia TPHK-2 and Acenitobacter sp. TPHK-3. P. putida TPHK-1 exhibited tolerance to very high concentrations of heavy metals such as cadmium, lead, zinc and copper. The innate ability of P. putida TPHK-1, as evidenced by the amplified genes alkB1 and alkB2 that encode alkane hydroxylases, and cat12o and cat23o coding for catechol dioxygenase, in degrading diesel oil in the presence of heavy metals is far greater than that of the strains reported in the literature. Heavy metal tolerance coupled with rapid degradation of hydrocarbons, even at high concentrations, suggests that P. putida TPHK-1 has a great potential in remediating soils contaminated with mixtures of hydrocarbons and heavy metals.  相似文献   

8.
The aim of this study was to evaluate the biosorption capacity of selected strains of microscopic fungi. We optimized the biosorption process and used the Freundlich isotherm for three strains: H. haematococca BwIII43, K37 and T. harzianum BsIII33 to describe the biosorption equilibrium of anthraquinone dye, Alizarin Blue Black B (ABBB) and alkali lignin (AL). In optimal conditions (1 g of mycelium biomass, pH = 7.0, 28 °C) for ABBB and AL sorption, the live biomass of H. haematococca BwIII43 was characterized by a higher sorption capacity, amounting to 247.47 and 161.00 mg g?1, respectively. The highest sorption properties toward anthraquinone dye (K F = 19.96 mg g?1) were shown for the biomass of H. haematococca K37. In the presence of alkali lignin, the highest sorption capacity and bond strength exhibited the biomass of H. haematococca BwIII43 (K F = 28.20 mg g?1, n = 3.46). Effective decolorization of ABBB and AL by the selected strains of microscopic fungi indicated that the biosorption process additionally enhanced the removal of color compounds from the solution.  相似文献   

9.
The aim of this study was to establish the seasonal variation of fungal propagules in homes of Mérida, Mexico, and to examine the relation between their concentrations in enclosed and open locations taking into account environmental parameters such as temperature, humidity and wind speed. Samples were taken inside and outside domestic environments during the most characteristic weather seasons in the region: dry season (sampled in May), rainy season (sampled in October), and cold fronts season (sampled in February). The geometric mean of the indoors and outdoors concentration of airborne fungal propagules was 1653 and 1432 CFU/m3 during the cold fronts period, 125 and 92 CFU/m3 during the dry period, and 1326 and 1145 CFU/m3 during the rainy period. The respirable fraction during the three periods sampled was always within a range of 50 to 100%. The composition of fungal genera exhibited a seasonal change, and 19 of these were identified. The main genera found during the three periods were Cladosporium spp., Penicillium spp., Aspergillus spp., Fusarium spp., andAcremonium spp. The fungal aerosol concentrations and diversity found in Mérida, Mexico, were similar to those from other reports of cities with comparable tropical humid climate. This study concludes that the concentration of airborne fungal propagules and its respirable fraction can be related to several meteorological factors, mainly to relative humidity.  相似文献   

10.
Radon and mercury concentrations were measured in 10 fault gas profiles in Generalized Haiyuan Fault. This paper aims to predetermine the potential seismic risk in different segments of the fault zone from the perspective of geochemistry. The background value and anomaly threshold were adopted and synthesized using the maximum value method and average method to calculate concentration intensity values of radon and mercury. Fault soil gas mercury and radon concentrations show a decreasing gradient from NW to SE indicating evident segmentation. Higher values are mostly distributed in the Maomao Mountain–Tiger Mountain fault and Jingtai area. Combined with the seismotectonic background of historical and recent earthquakes and the spatial distribution characteristics of b-values, the fault soil gas concentration intensity shows a close correlation with earthquake activity within the fault zone. Concentrations of fault gas are higher and the b-value lower in areas of strong seismic activity, and regions with weak seismic activity correspond to lower fault gas concentrations and higher b-values. It is thus considered that the Jingtai area may be more dangerous than the other areas. This paper could provide vital background information for earthquake prediction in the Generalized Haiyuan Fault Zone.  相似文献   

11.
This study presents both qualitative and quantitative data regarding marine mollusk (gastropods and bivalves) shell bioerosion and encrustation based on death assemblages obtained from a recent supratidal environment in Playa Norte, Veracruz State. The objectives of this study were to assess the nature of bioerosion and encrustation processes and to investigate the role of these taphonomic features contributing to the deterioration of natural shell accumulations within a tropical siliciclastic tidal environment. The assemblage comprises 31 species: 13 gastropods and 18 bivalves. The bioerosion and encrustation degrees were low to moderate for both types. The most abundant traces were predatory gastropod structures (Oichnus paraboloides and O. simplex), whereas sponge borings (Entobia isp.), polychaete dwellings (Caulostrepsis taeniola), and echinoid raspings (Gnatichnus isp.) were less frequent. The encrusting organisms include polychaete serpulids, bryozoans, and rare foraminifers (Homotrema rubrum). Because of the low bioerosion and encrustation degrees occurring in this area, accumulation is expected to predominate over biotic destruction. As deposition conditions (richly fossiliferous carbonate sandstone beds) were similar to those prevailing in the Tuxpan Formation during the Miocene (Langhian), it is suggested that this study provides an equivalent reference to interpret mollusk fossil assemblages located in this site.  相似文献   

12.
The identified mollusks from the Burullus lagoon of Egypt (Eastern Mediterranean) are represented by four bivalve species and four gastropod species. Sources of heavy metals pollution in the Burullus lagoon include phosphate fertilizers, sewage and oil spills from fishing boats.The Cerastoderma glaucum species has a wide distribution in the central part of the studied area with satisfying criteria required for good bio-monitors of pollution. High concentrations of heavy metals are found in water, at the eastern side of the lagoon, while low concentrations were detected in the central and western sides of the lagoon. Pirenella conica constitutes the total gastropods assemblages, reflecting this species tolerance to the high concentration of heavy metals. Meanwhile, the total pelecypods in bottom lagoon sediments are completely absent in the eastern parts of the lagoon, which may be due to the sensitivity of pelecypods to the accumulation of heavy metals.  相似文献   

13.
The aim of this research was to identify plant species with potential to accumulate and stabilize arsenic (As), lead (Pb) and cadmium (Cd) in mine tailings reforested and naturally recolonized locations in a semiarid region of Zacatecas, Mexico. Plant shoots from 44 species and their rhizospheric soils were analyzed for As, Pb and Cd concentration using atomic absorption spectroscopy. Most represented plant families were Asteraceae, Poaceae, Fabaceae and Cactaceae. The highest concentrations in shoots were As, followed by Pb and Cd. Among herbaceous species, Bouteloua gracilis showed the highest bioconcentration factor (BCF) of As, while Plantago lanceolata showed the highest bioconcentration factor of Pb. The shrub species with highest concentration of As in the rhizospheric soil were Opuntia robusta, Melilotus alba, Baccharis neglecta and Arundo donax (near BCF to 1.0). Similar results were observed in trees Casuarina equisetifolia, Prosopis laevigata, Fraxinus uhdei and Eucalyptus globulus. In addition, Tillandsia recurvata showed a suitable indicator of atmospheric deposition to As. In general, the results suggest that these species can be effective for tailings reforestation with the possibility to enclose potentially toxic elements. Specially, C. equisetifolia which is abundant, having the potential for future applications in other contaminated sites with different types of mine tailings or abandoned mines from arid and semiarid zones.  相似文献   

14.
Collocated measurements of the optical and physical properties of columnar and near-surface aerosols were carried out from Manora Peak, Nainital (a sparsely inhabited, high altitude location, ～2 km above mean sea level, in the Himalayas), during the Integrated Campaign for Aerosols, gases and Radiation Budget (ICARB) under the Geosphere Biosphere Programme of the Indian Space Research Organization (ISRO-GBP). Under this, observational data of spectral aerosol optical depths (AOD), mass concentration of aerosol black carbon (M B ), mass concentration (M T ) and number concentration (N t ) of composite (total) aerosols near the surface and meteorological parameters were collected during the period February 15 to April 30, 2006. Though very low (<0.1 at 500 nm) AODs were observed during clear days, as much as a four-fold increase was seen on hazy days. The Ångström exponent (α), deduced from the spectral AODs, revealed high values during clear days, while on hazy days α was low; with an overall mean value of 0.69 ± 0.06 for the campaign period. BC mass concentration varied between 0.36 and 2.87 μg m?3 and contributed in the range 0.7 to 1.8% to the total aerosol mass. Total aerosol number concentration and BC mass concentration showed diurnal variation with a midnight and early morning minimum and a late afternoon maximum; a pattern quite opposite to that seen in low altitude stations. These are attributed to the dynamics of the atmospheric boundary layer.  相似文献   

15.
Hexavalent chromium [Cr(VI)] is used in various industries, but its improper and uncontrolled discharge contaminates the environment. In order to circumvent chromium toxicity, several physicochemical and biological strategies have been employed. Among biological approach, microbes convert toxic Cr(VI) to less soluble Cr(III) form and hence can be used to detoxify/remove Cr(VI) from contaminated environment. Considering these, present study was designed to assess the effect of chromium reductases and antioxidants secreted by Penibacillus species to detoxify Cr(VI) and concurrently to augment soybean growth. Bacterial strains (MAI1 and MAI2) were identified as Penibacillus sp. using 16S rRNA gene. Penibacillus species reduced Cr(VI) significantly at pH 7. Maximum Cr(VI) was reduced at 50 and 100 µg/ml of Cr(VI) concentrations. Penibacillus sp. also reduced Cr(VI) significantly at 25 and 35 °C as well as 1 g sodium alginate in 1 g polyvinyl alcohol. Bacterial strains reduced Cr(VI) into Cr(III) which were detected as 33 ± 1 and 35 ± 1 µg/ml in supernatant and 67 ± 2.5 and 65 ± 1 µg/ml in cell debris, respectively, after 120 h. Chromium reductase found in cell-free extract reduced almost all Cr(VI) compared to those observed in cell debris. Both malondialdehyde and antioxidant levels were increased with gradual increase in Cr(VI) concentration. Penibacillus species inoculated soybean plants had better growth and photosynthetic pigments under Cr(VI) stress.  相似文献   

16.
There is currently limited research available on the secondary metabolites of moulds in workplaces. The aim of this study was to determine the mould contamination in museums (N = 4), composting plants (N = 4) and tanneries (N = 4) and the secondary metabolite profiles of Alternaria, Aspergillus and Penicillium isolates from these workplaces. Alternaria, Aspergillus and Penicillium species were identified using the ITS1/2 sequence of the rDNA region. Mould metabolites were quantitatively analysed on standard laboratory medium and mineral medium containing materials specific to each workplace using liquid chromatography-mass spectrometry. We also examined the cytotoxicity of the moulds using MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assays. Air microbiological contamination analyses showed a number of microorganisms, ranging from 2.4 × 103 CFU m?3 (composting plants) to 6.8 × 104 CFU m?3 (tanneries). We identified high percentages of Alternaria, Aspergillus and Penicillium moulds (air 57–59%, surfaces 10–65%) in all workplaces. The following moulds were the most cytotoxic (>90%): Alternaria alternata, A. limoniasperae, Aspergillus flavus, Penicillium biourgeianum, P. commune and P. spinulosum. The same mould species isolated from different working environments exhibited varying toxigenic and cytotoxic properties. Modifying the culture medium to simulate environmental conditions most often resulted in the inhibition of secondary metabolite production. Moulds isolated from the working environments produced the following mycotoxins (ng g?1): chanoclavines (0.28–204), cyclopiazonic acid (27.1–1045), fumigaclavines (0.33–10,640,000), meleagrin (0.57–13,393), roquefortins (0.01–16,660), rugulovasines (112–220), viridicatin (0.12–957), viridicatol (4.23–2753) and quinocitrinines (0.07–1104), which may have a negative impact on human health.  相似文献   

17.
In the present study, a widely used reactive dye, Color Index (C.I.) Reactive Blue 268 was utilized for mycoremediation by Aspergillus fumigatus isolated from textile effluent. Complete decolorization of the test dye (0.1 g L?1) was recorded within 6 days of static incubation at 27 °C in Czapek Dox broth (CDB). However, the isolate was unable to utilize the dye as a sole source of energy in Czapek Dox agar and CDB in absence of sucrose and obligate requirement of a labile carbon source, i.e., sucrose needed for induction of decolorization. Biosorption seems to play the pivotal role in decolorization as evident by coloring of the fungal biomass as that of dye color. The optimal conditions for the highest decolorization were found at 30 °C and pH 6.0 with 6-day-old inoculums supplemented with sucrose (10 g L?1) and ammonium chloride (2 g L?1) as a carbon and nitrogen source, respectively. The response of the isolate to increasing dye concentrations was found to be growth inhibitory. Surprisingly, about 65 % of dye decolorization was recorded with heat-inactivated biomass powder within 6 days of static incubation supporting the fact of fungal biosorption. Results of this study have established the candidature of the isolate for biotechnological removal of dyes from disreputable dying effluents.  相似文献   

18.
Mass concentration and mass size distribution of total (composite) aerosols near the surface are essential inputs needed in developing aerosol models for radiative forcing estimation as well as to infer the environment and air quality. Using extensive measurements onboard the oceanographic research vessel, Sagar Kanya, during its cruise SK223B in the second phase of the ocean segment of the Integrated Campaign for Aerosols, gases and Radiation Budget (ICARB), the spatial distribution of the mass concentration and mass size distribution of near-surface aerosols are examined for the first time over the entire Arabian Sea, going as far as 58°E and 22°N, within a span of 26 days. In general, the mass concentrations (M T ) were found to be low with the mean value for the entire Arabian Sea being 16.7 ± 7 μg m?3; almost 1/2 of the values reported in some of the earlier campaigns. Coarse mode aerosols contributed, on an average, 58% to the total mass, even though at a few pockets accumulation mode contribution dominated. Spatially, significant variations were observed over central and northern Arabian Sea as well as close to the west coast of India. In central Arabian Sea, even though the M T was quite low, contribution ofs accumulation aerosols to the total mass concentration was greater than 50%. Effective radius, a parameter important in determining scattering properties of aerosol size distribution, varied between 0.07 and 0.4 μm with a mean value of 0.2 μm. Number size distributions, deduced from the mass size distributions, were approximated to inverse power-law form and the size indices (ν) were estimated. It was found to vary in the range 3.9 to 4.2 with a mean value of 4.0 for the entire oceanic region. Extinction coefficients, estimated using the number-size distributions, were well-correlated with the accumulation mode mass concentration with a correlation coefficient of 0.82.  相似文献   

19.
The accumulation efficiency of selected trace elements in the leaves of Melandrium album and Robinia pseudoacacia grown on heavy metal contaminated sites in comparison with a non-contaminated one was evaluated. The study was undertaken to calculate air pollution tolerance index and to determine the contents of selected metabolites: glutathione, non-protein thiols, ascorbic acid, chlorophyll and the activity of antioxidant enzymes: guaiacol peroxidase and superoxide dismutase. Such estimations can be useful in better understanding of plants defense strategies and potential to grow in contaminated environments. The results in the most contaminated site revealed higher contents of metals in M. album leaves, especially Zn, Cd and Pb (3.4, 6 and 2.3 times higher, respectively) in comparison with the R. pseudoacacia. Better accumulation capacity found in M. album was shown by metal accumulation index values. The plants could be used as indicators of Zn, Cd (both species) and Pb (M. album) in the soil. Glutathione content (in both species) and peroxidase activity (in M. album), general markers of heavy metals contamination, were increased in contaminated sites. In most cases in contaminated areas R. pseudoacacia had decreased ascorbic acid and chlorophyll levels. Opposite tendency was recorded in M. album leaves, where similar or higher contents of the above-mentioned metabolites were found. In our study, M. album and R. pseudoacacia proved to be sensitive species with the air pollution tolerance index lower than 11 and can be recommended as bioindicators.  相似文献   

20.
The feeding behavior of three species of mussels, the native Ischadium recurvum and the invasives Mytella charruana and Perna viridis, was studied in an invaded ecosystem in Florida (USA). In situ feeding experiments using the biodeposition method were performed along a salinity gradient in four different locations along the St. Johns River. Water characteristics, such as salinity, temperature, dissolved oxygen, and seston loads, were recorded to identify relationships between these variables and the feeding behavior of the mussels. Feeding behavior of the species varied by study site. Clearance, filtration, organic ingestion, and absorption rates of I. recurvum were negatively affected by salinity. For the invasive mussel, M. charruana, rejection was positively related to salinity while total ingestion, organic ingestion, and absorption rates were positively related to the percentage of organic matter in the seston. For P. viridis, total and organic ingestion rates were negatively affected by salinity but positively affected by total particulate matter. Condition indices for P. viridis and M. charruana were 13.16?±?0.64 and 6.63?±?0.43, respectively, compared to 4.82?±?0.41 for the native species I. recurvum, indicating that these mussels are well adapted to the environmental conditions in the area. This study indicates that the three species have different preferred habitats because of their specific responses to water characteristics. Thus, the invasive mussels will not totally occupy the niche of the native mussel in Florida despite overlapping zones. These data may help identify potential invaded areas and understand the extent of the invasion.  相似文献   

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