共查询到14条相似文献，搜索用时 62 毫秒
1.
研究了大豆浓缩蛋白作为饵料蛋白源对中华绒螯蟹幼蟹消化酶活力的影响,初步探讨了中华绒螯蟹对饵料蛋白源的内在适应机理。根据中华绒螯蟹的营养需求,用大豆浓缩蛋白替代鱼粉作为蛋白源配制成6种近似等蛋白等能的饵料,其中大豆浓缩蛋白在饵料中的含量分别为0、11%、22%、33%、44%和56%(分别替代鱼蛋白质量的0、18.64%、37.93%、56.90%、77.19%和100%),对照饵料全部以鱼粉作为蛋白源。用上述6种饵料喂养中华绒螯蟹50 d后,测定蟹肝胰腺各种消化酶活力。结果表明:随着饵料中大豆浓缩蛋白含量的升高,胃蛋白酶和胰蛋白酶活力均呈降低之势。当大豆浓缩蛋白含量达到44%时,与全鱼粉对照组相比,胃蛋白酶和胰蛋白酶活力显著性降低(P<0.05);同时,淀粉酶稍呈升高之势,而纤维素酶则稍呈降低的趋势;A/T比例呈显著上升的趋势,当大豆浓缩蛋白含量达44%时,与对照组有显著性差异,说明河蟹对植物蛋白有很强的适应性。 相似文献
2.
中华绒螯蟹是我国水产养殖上的主要养殖品种之一 ,但其苗种供应和成蟹产量仍不稳定 ,特别是大规格成蟹产量非常低 ,这成为河蟹养殖的一个制约因素。对河蟹消化酶的研究 ,有助于揭示河蟹在各个养殖阶段和环境下的营养需求特点。关于蟹类消化酶的研究 ,仅在幼体方面有些报道 ,如Hirche和 Anger测定了蛛形蛤蟆蟹各期幼体消化酶活力 [1] ;国内学者潘鲁青等研究了中华绒螯蟹幼体的消化酶活性与氨基酸组成[2 ] ;汤鸿等研究了锯缘青蟹幼体消化酶活力。本文测定了中华绒螯蟹幼体及成蟹的消化酶活性 ,试图从消化酶角度探讨中华绒螯蟹的营养需求以及… 相似文献
3.
采集日本冲绳源河川和边野古川的日本绒螯蟹样品参考果蝇与蚤状线粒体ＤＮＡ１２ｒＲＮＡ基因片段序列进行了其相同片段的引物设计、ＰＣＲ扩增及序列测定。结果表明冲绳两河川４只日本绒螯蟹的碱基序列完全相同，为４５８ｂｐ，其Ａ、Ｔ、Ｇ、Ｃ含量分别为１６０ｂｐ（３４．９４％）、１７９ｂｐ（３９．０８％）、４９ｂｐ（１０．７０％）、７０ｂｐ（１５．２８％）同果与蚤状蚤相同基因片段的序列含量相似。 相似文献
4.
以小杂鱼、低值鱼为原料，采用溶剂萃取法研究了溶剂种类、温度、时间和溶剂量对鱼蛋白提取过程中脱水、脱脂、脱色和脱腥的影响，筛选出提取浓缩鱼蛋白的最佳工艺条件。结果表明，在该工艺条件下制得的浓缩鱼蛋白，其蛋白质含量高达９２．２８％，脂肪含量仅为０．０９２％。 相似文献
5.
利用小杂鱼,低值鱼提取浓缩鱼蛋白 总被引：9，自引：0，他引：9
以小杂鱼、低值鱼为原料，采用溶剂萃取法研究了溶剂种类温度、时间和溶剂量对鱼蛋白提取过程中脱水、脱脂、脱色和脱腥的影响，筛选出提取浓缩鱼蛋白最佳工艺条件。结果表明：在该工艺条件下制得的浓缩鱼蛋白提取其蛋白质含量达９２．２８％，脂肪含量仅为０．０９２％。 相似文献
6.
《广东海洋大学学报》2017,(6)
在以鱼粉、豆粕、棉籽粕和双低菜籽粕为蛋白源的实用饲料中分别添加植酸酶1 000 IU/kg和复合酶制剂400 mg/kg,饲喂初始体质量为(1.02±0.01)g的黄颡鱼稚鱼56 d,探讨外源酶制剂对稚鱼消化酶活性、消化率及粪便氮、磷含量的影响。结果表明:与对照组比较,二实验组稚鱼胃蛋白酶活性均高于对照组(P0.05),植酸酶组稚鱼肠道蛋白酶、胃淀粉酶活性高于对照组,差异有统计学意义(P0.05)。植酸酶组稚鱼对饲料干物质、粗蛋白质、总能、灰分和磷表观消化率高于对照组,灰分和磷表观消化率也高于复合酶组,差异均有统计学意义(P0.05);复合酶组稚鱼对饲料粗脂肪、总能表观消化率高于对照组,差异有统计学意义(P0.05)。实验组稚鱼粪便氮含量与对照组均差异无统计学意义(P0.05);植酸酶组稚鱼粪便磷含量低于对照组,差异有统计学意义(P0.05)。在稚鱼实用饲料中添加植酸酶可提高消化道消化酶活性,提高其对饲料营养物质的表观消化率,减少粪便磷排放。 相似文献
7.
This work conducted a four-week metabolism test on rats to study the digestion and absorption characteristics of five protein-based krill products prepared from Antarctic krill as raw material. It aimed to provide theoretical support for the effective use of Antarctic krill protein and the development of novel protein resources. The results showed that the weight gain and true digestibility of the rats fed with krill meat, surimi and ordinary krill powder were significantly higher(P < 0.05) t... 相似文献
8.
Effects of Dietary Protein Level on Growth and Utilization of Protein and Energy by Juvenile Mangrove Red Snapper (Lutjanus argentimaculatus ) 总被引：1，自引：1，他引：0
1Introduction The mangrove red snapper,Lutjanus argentimacu-latus(Forsskal,1775)is a carnivorous,warm-watereuryhaline fish that is considerably cultured in South-east Asia,Southern China and the Middle East(Le-ung et al.,1999;Estudillo et at.,2000;Ng et al.,2000;Catacutan et al.,2001).In Pakistan,it isknown for its good quality meat and also for its highconsumption rate.Although it fetches a premiumprice at local markets(Anonymous,2002),the in-creasing demand has generated interest towar… 相似文献
9.
《中国海洋大学学报(英文版)》2016,(3)
Poor palatability is a limiting factor for replacing fishmeal with other protein sources in aquaculture. The water-soluble molecules with low molecular weights are the major determinants of the palatability of diets. The present study was conducted to investigate the palatability of water-soluble extracts from single protein source(single extract pellets) and the mixture of these extracts with different proportions(blended extract pellets) in juvenile turbot(Scophthalmus maximus). Then according to the palatability of blended extract pellets, an optimal mixture proportion was selected, and a new protein source made from raw protein materials with the selected proportion was formulated to replace fishmeal. Summarily, the palatability of single extract pellets for turbot was descendent from fishmeal to pet-food grade poultry by-product meal, wheat gluten meal, soybean meal, peanut meal, meat and bone meal, and corn gluten meal. Subsequently, according to the palatability of single extract pellets, 52 kinds of blended extract pellets were designed to test their palatability. The results showed that the pellets presented remarkably different palatability, and the optimal one was diet 52(wheat gluten meal: pet-food grade poultry by-product meal: meat and bone meal: corn gluten meal = 1:6:1:2). The highest ingestion ratio(the number of pellets ingested/the number of pellets fed) was 0.73 ± 0.03, which was observed in Diet 52. Then five isonitrogenous(52% crude protein) and isocaloric(20 k J g~(-1) gross energy) diets were formulated by replacing 0(control), 35%, 50%, 65% and 80% of fishmeal with No.52 blending proportion. After a 10-weeks feeding trial, a consistent feed intake was found among all replacement treatments. Replacement level of fishmeal up to 35% did not significantly influence final body weight, specific growth rate, feed efficiency ratio, and protein efficiency ratio of turbot. Therefore, the water-soluble extracts of protein sources play an important role in improving the palatability of non-fishmeal protein sources in aquafeed. 相似文献
10.
11.
青鳞鱼蛋白复合酶控制水解动力学模型的研究 总被引：1，自引：0，他引：1
在假设复合酶恒温控制水解动力学遵循内切酶限制水解动力学历程的前提下 ,采用实验方法求出了复合酶恒温控制水解动力学模型。结果表明 ,复合酶对青鳞鱼蛋白进行控制水解的动力学模型为 :R =(0 .315 4e0 - 0 .0 186s0 )exp(- 0 .170DH) ,DH =5 .882ln[1+(0 .0 5 36 2e0 /s0 -0 .0 0 32 )t];其酶失活常数Kd=0 .0 5 36min- 1;水解反应能够顺利进行的条件是 :e0 /s0 >c0 ,常数c0 =5 .90× 10 - 2 。验证实验证明 ,根据复合酶恒温控制水解动力学模型得到的理论DH与实际DH基本吻合 ,该动力学模型具有很强的实用价值。 相似文献
12.
The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein(47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0℃, salinity was controlled between 28.5 and 32.0, acidity was controlled between p H7.8 and p H8.5, and ammonia nitrogen was maintained below 0.03 mg L-1 and dissolved oxygen was maintained about 7 mg L-1. Results showed that the growth of fish was significantly affected by dietary protein content(P 0.05). Specific growth rate(SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0%(P 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets(P 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%. 相似文献
13.
研究了木瓜蛋白酶的酶解温度、酶浓度、酶解时间、加水量、pH值对青鳞鱼可溶性蛋白得率的影响 ,探讨了α 氨态氮含量与青鳞鱼可溶性蛋白得率的关系。结果表明 :温度小于 5 0℃ ,随着酶解温度的提高、酶浓度的增加、酶解时间的延长青鳞鱼可溶性蛋白得率及α 氨态氮的含量均增加 ,α 氨态氮含量增加比可溶性蛋白得率的增加明显 ;加水量的增加 ,可溶性蛋白得率增加 ;pH值对水解效果及可溶性蛋白得率影响不大。综合考虑 ,最佳水解条件为 :温度 5 0℃ ,酶浓度为 1 5 0 0u/g ,酶解时间 80min ,m原料 :m水 =1∶2 ,pH 6 8。酶解所得可溶性蛋白经氨基酸分析仪检测 ,其氨基酸总量为 49 47mg/mL ,必需氨基酸占氨基酸总量的 41 0 % ,游离氨基酸含量为 6 3mg/mL ,肽含量为 87 2 %。 相似文献
14.
以重组质粒pMD18-T/IL6和pMD18-T/OmpW为模板,分别扩增红笛鲷IL-6基因和哈维氏弧菌外膜蛋白OmpW基因,运用PCR重叠延伸剪切技术,将IL-6和OmpW基因融合,将融合基因定向克隆到原核表达载体pET-32a(+),转化大肠杆菌BL21(DE3)感受态,经异丙基-β-D-硫代半乳糖苷(IPTG)诱导融合蛋白高效表达,融合蛋白分子质量约为66.6 ku。优化后表达条件为温度37℃,IPTG浓度0.2 mmol·L-1,诱导时间5 h。用HisTrap HP亲和柱纯化重组蛋白,最佳咪唑洗脱浓度为400 mmol·L-1,纯化蛋白的质量浓度为480μg·mL-1。Western-blot分析显示,该融合蛋白可与鼠抗His-tag单克隆抗体发生特异反应,表明目的蛋白得以正确表达。 相似文献