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1.
利用透射电子显微镜观察乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构。结果表明,乌苏里拟鲿精子由头部、中段和尾部组成。头部呈球形或椭圆球形,核前端无顶体,后端有植入窝。中段包括中心粒复合体和袖套。植入窝从细胞核的后端陷入核中。中心粒复合体位于植入窝内,包括近端中心粒和远端中心粒。袖套与细胞核后端相连,袖套中含有丰富的线粒体。尾部主要结构是轴丝,为典型的"9+2"结构,轴丝外两侧为细胞质膜形成的对称性鳍样结构。尾部内无囊泡及其他细胞器。  相似文献   

2.
盐度及pH对黄鳍东方鲀受精卵孵化和仔鱼活力的影响   总被引:3,自引:1,他引:3  
比较了不同盐度(5、10、15、20、25、30、35、40、45)及pH条件(5.0、5.5、6.0、6.5、7.0、7.5、8.0、8.5、9.0、9.5)下黄鳍东方鲀受精卵的培育周期、孵化周期和孵化率,同时对黄鳍东方鲀初孵仔鱼进行耐饥饿试验,测定其不投饵存活系数SAI值。结果表明,黄鳍东方鲀受精卵孵化的适宜盐度范围是5.0~40.0,适宜pH范围是6.0~8.5;仔鱼生存的适宜盐度为5.0~45.0,适宜pH值是5.5~9.0。  相似文献   

3.
采用半静水生物测试法,研究亚硝酸盐氮对红鳍东方鲀(Takifugu rubripes)(9.59.8 g)的急性毒性及对部分肝脏抗氧化酶活性的影响。结果表明,在急性毒性实验中,亚硝酸盐氮对红鳍东方鲀的24、48、72和96 h半致死质量浓度(LC50)分别为201.88、190.57、171.09、159.25 mg/L,安全质量浓度为15.92 mg/L;在亚急性毒性实验中,当红鳍东方鲀分别在5.3、10.6,15.9、21.2 mg/L亚硝酸盐氮中暴露4周时,其肝脏总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性随亚硝酸盐氮浓度的升高均被显著抑制,差异有统计学意义(P<0.05),表明亚硝酸盐氮会对鱼体的抗氧化能力产生影响。  相似文献   

4.
对低盐胁迫下体质量(11.34±2.13)g红鳍东方鲀(Takifugu rubripes)的鳃、肾和肠组织结构进行显微观察,探讨组织结构变化与渗透压的关系。结果表明,低盐胁迫下,鳃丝和鳃小片宽度增大,鳃小片间距缩小,与对照组差异均有统计学意义(P0.05),鳃小片细胞更为圆润饱满,低盐度组每100μm泌氯细胞数为0.91±0.78,低于对照组,差异有统计学意义(P0.05);低盐度组的肾小球膨大,饱满充盈,与肾小囊内壁间隙小于对照组,差异有统计学意义(P0.05),近曲小管和集合管管径增大,近曲小管游离面刷状缘较对照组发达;低盐度组肠绒毛单层柱状上皮厚度为(51.54±2.53)μm,大于对照组(P0.05),柱状上皮细胞间每100μm杯状细胞数为5.05±0.91,小于对照组(P0.05),而两组间的杯状细胞大小差异无统计学意义(P0.05)。水体盐度变化导致红鳍东方鲀鳃、肾和肠发生适应性变化。  相似文献   

5.
【目的】揭示红鳍笛鲷(Lutjanus erythropterus)红体色的分布特征及影响机制。【方法】利用色差仪分别测定背部、腹部和尾部皮肤的体色值,用形态学观察、石蜡切片、冰冻切片和透射电镜切片等方法分析色素细胞组成、分布和数量特征,并使用紫外分光光度法和液相色谱-质谱/质谱法研究类胡萝卜素和体色特征的关系。【结果与结论】红鳍笛鲷体表的红色素细胞显著多于黑色素细胞(P <0.001),且从背部向腹部逐渐减少,红体色受到黑色素细胞分布的影响;皮肤和视网膜是类胡萝卜素的主要沉积部位,皮肤中类胡萝卜素以脂肪酸结合的形式存在,其中红色类胡萝卜素主要有虾青素、角黄素和β-柠乌素等3种,虾青素和角黄素含量与红色素细胞数量呈正相关。建议在饲料中补充虾青素和角黄素以提升红色。  相似文献   

6.
连巴岭铅锌矿区位于太行山北段涞源花岗岩体北侧与阜平变质核杂岩北西缘,产于乌龙沟—上黄旗深断裂带的次级平行断裂带中。分析了连巴岭铅锌矿床成矿地质背景、矿相学特征以及控矿构造,对比研究了矿石S-Pb同位素组成,探讨了成矿物质来源,建立了成矿模式。结果表明:金属硫化物样品(黄铁矿、闪锌矿、黄铜矿、方铅矿)的硫同位素组成为(-2.7~6.9)×10-3,多数集中在(-1~3)×10-3,平均值为1.1×10-3,与地幔硫接近;硫同位素组成频率直方图呈塔式分布,表明其深源岩浆硫的特点;矿石铅同位素组成稳定,花岗岩是主要的成矿母岩,成矿物质具有壳幔混合特点,并来源于上地幔—下地壳物质部分熔融形成的岩浆。连巴岭铅锌矿床成矿模式为:岩层因受到多种构造应力的影响而发生变形和破裂;中生代燕山运动引起次一级的褶曲断裂和节理,使地壳变得更加虚弱;基底的王安镇岩体发生断裂,花岗岩体乘机侵入;然后酸性、中性、基性岩脉侵入,由于颗粒细小,近于致密,渗透性差的中性岩脉起到屏蔽、遮挡作用,使得含矿热液与白云岩发生交代作用而聚集堆积形成矿体。  相似文献   

7.
湖南炎陵印支期隔槽式褶皱形成机制   总被引:19,自引:0,他引:19  
为查明湘东南印支期褶皱特征及形成机制,对炎陵地区进行了详细的地质填图和野外观察。调查表明,泥盆纪跳马涧组与前泥盆纪褶皱基底间不整合界面在空间上组成背、向斜,表明褶皱基底参与了印支期褶皱作用。平面上,向斜出露宽度及延伸规模明显较背斜小,向斜转折端表现清楚且狭窄尖长,而背斜转折端没有清楚显示;剖面上,不整合界面靠近向斜核部产状陡,向两翼方向迅速变缓。反映出背斜平缓开阔、向斜紧闭狭窄且侧翼呈上拱尖棱状的隔槽式褶皱特征。印支期褶皱呈北北东向,横跨在前泥盆纪褶皱基底中加里东期北西向褶皱之上,但未对加里东期褶皱造成明显地叠加改造效应,说明前泥盆纪地层在印支运动中没有发生明显地弯滑褶皱作用。露头显示,褶皱过程中盖层未沿不整合界面产生明显滑脱。因此,认为炎陵地区隔槽式褶皱形成于基底(厚皮式)横向收缩与压扁作用,并从理论上论证了其隔槽式褶皱不可能是盖层沿基底滑脱的产物,纠正了前人的薄皮式观点。  相似文献   

8.
洒西钨铍矿床位于滇东南老君山钨锡多金属成矿区。确定早期似层状矿体和晚期脉状矿体的流体性质、来源和演化过程, 并对其流体地球化学和同位素进行约束, 可以有效探讨洒西钨铍矿床成矿机制, 为老君山矿集区下一步找矿工作提供理论思考。洒西钨铍矿床脉状矿体的形成经历了硅酸盐阶段、氧化物-硫化物阶段和碳酸盐-萤石阶段, 白钨矿和绿柱石主要形成于前两阶段。对洒西钨铍矿床脉状矿体不同阶段石英中的流体包裹体进行了岩相学特征、显微测温、激光拉曼光谱分析等研究, 并对早期似层状矿体和晚期脉状矿体矿石中石英的氢-氧同位素组成进行了测试。结果表明: 该矿床内脉状矿体的流体包裹体主要有富液相包裹体、富气相包裹体和含子矿物多相包裹体3种类型。流体包裹体的气相成分以H2O主, 含少量还原性气体如C2H2等, 液相成分也以H2O为主。从硅酸盐阶段到碳酸盐-萤石阶段, 包裹体的均一温度和盐度(NaCleq)峰值范围分别为240~360℃、2.35%~13.81%;220~310℃、4.03%~9.86%和190~270℃、2.41%~6.88%。从硅酸盐阶段到碳酸盐-萤石阶段, 成矿流体的温度呈现降低趋势, 盐度也呈降低趋势。成矿流体总体上属中-高温度、低盐度、贫CO2、含部分还原性气体的NaCl-H2O流体体系。早期似层状矿体石英样品δDV-SMOW值变化范围小, 为-102.8‰~-99.0‰, δ18OV-SMOW值为11.7‰~13.0‰, δ18OH2O值为3.16‰~6.46‰; 晚期脉状矿体氧化物-硫化物阶段石英样品δDV-SMOW值变化范围较大, 为-99.6‰~-69.5‰, δ18OV-SMOW值为11.2‰~14.1‰, δ18OH2O值为3.08‰~6.73‰。综合表明成矿流体主要是岩浆水, 混合有少量大气降水或有机水, 流体可能发生了沸腾作用, 加之温度的降低, 导致晚期脉状矿体氧化物-硫化物阶段主要成矿物质的沉淀。洒西钨铍矿床属于中高温热液矿床。   相似文献   

9.
Transmission electron microscopy of the yellowfin porgy (Sparus latus Houttuyn) spermatozoa ultrastructure showed the spermatozoon as a primitive type made up of the acrosomeless head, the flagellum, and the midpiece, at the periphery of which was a relatively big mitochondrion with more complex structure. It was found that during spermiogenesis, only one relatively big mitochondrion occurred in both the spermatid and the spermatozoon. This is different from other teleost fishes. During spemiogenesis, the mitochondria number is one, and morphology did not change. All these are different from those of other fishes.  相似文献   

10.
ImODUcrlONTheultmetruCtureofthespermatosoainteeostfisheshaddridesedtalandcomParedpelllard,l97o,Mattei,l97O).ComPatalwithotherVertebrausryisheIson,l995),FisheShavegreaterdriversityinspermatosoaultfastruCture,indudingthemorphologicalcharaCteristicaofthesperm-headsophnsteinerandPatZner,l99O),thefonnswithorwithoutacrosorapeisheIson,l995),thenumberoftheflagellum,andthedistinguishingfeaturesofAntochondrialderivatives(JawhesonandGrier,l993).AllthesearereataltotheFishposi-tioninevolutionandt…  相似文献   

11.
Morphology of the spermatozoon of Octopus ocellatus was studied by light, scanning electron, and transmission electron microscopes. Sperm are 600–700 μm long, with a large number of granules in diameter about 130 nm. Each spermatozoon is composed of a head, neck, and tail. The head is made up of an acrosomal complex anterior to the nucleus. The spiral acrosomal complex consists of an electron-lucent vesicle, lacuna, and an electron-dense acrosomal vesicle. Additionally, the spiral acrosomal vesicle has numerous equidistant striations, and is surrounded by many small granules (20 nm diameter). A long straight nucleus, which is electron-densed, has a deep posterior concavity, the nuclear vacuole. At the terminal end of the nucleus is a sleeve-like structure with a concave posterior nuclear fossa (PNF). The neck is short connecting the PNF. The basal body is located in the PNF and gives rise to the axoneme. This structure connects the head, neck, and tail. The tail is divided into a middle piece and a principal piece. The middle piece, having a 9+9+2 arrangement, is surrounded by a mitochondrial sheath and terminates by an electron-dense fibrous sheath. The principal piece is the longest part of the sperm with coarse fibers tapering posteriorly. The results of this study shall provide some useful information for artificial breeding of this species.  相似文献   

12.
3种珍珠贝精子发生及其超微结构的比较研究   总被引:1,自引:0,他引:1  
在透射电子显微镜下观察合浦珠母贝(Pinctadafucata)、大珠母贝(Pinctadamaxima)和企鹅珍珠贝(Pteriapenguin)的精子发生及精子形态.结果表明.大珠母贝从精原细胞到精子,核内都有数量不等的板层小体(nuclearlamellarbody);企鹦珍珠贝从精原细胞的晚期直到精子细胞早期,大多数细胞出现核内包含体(nuclearinclusion);而合浦珠母贝的生精细胞核无特殊结构.合浦珠母贝在初级精母细胞时由高尔基体分泌形成前顶体粒;企鹅珍珠贝的前顶体粒主要在次级精母细胞和早期精子细胞阶段形成。虽然大珠母贝的各级生精细胞中都没有出现典型的高尔基体,但前顶体粒仍在精母细胞时期形成。三种贝的精子结构均为典型的原生型,由头部、中段和尾部构成.合浦珠母贝精子的顶体最长,中段的线粒体数有变异;大珠母贝的精子核最高,其中含有板层小体;而企鹅珍珠贝精子中段的体积是最大的.  相似文献   

13.
INTRODUCTIONBillard ( 1 986)studiedthespermatozoonultrastructuresinteleostsandfounddistinctdifferencesa mongdifferentspecies.Studyonspermatozoonmorphologyandfunctionisachallengebecauseofthesperm’sspecialenvironment (Gageetal.,1 998;WangandZhang,1 999) .Sinceleft ey…  相似文献   

14.
Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy (TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.  相似文献   

15.
The spermatozoon ultrastrucmre of left-eye flounder, Paralichthys olivaceus, was ob-served by electronic microscopy, and shown to consist of head, midpiece and tail. Some nuclear vacuoles were observed inside and along the outer part of the nucleus and double-membrane structures were ob-served between the nuclear membrane and plasma membrane. The sperm sleeve consisted of four indepen-dent parts and had more than four mitochondria. Along the sperm tail there were wing-like lateral tins.  相似文献   

16.
This study was conducted to clarify the distribution and morphology of free neuromasts during the development of half-smooth tongue sole(Cynoglossus semilaevis) using scanning electron microscopy.During development,(1) the apical surface of free neuromasts changed in shape from a circle to a four-point star;(2) the external structure changed from being level with the epidermis to papilla-like above the level of the epidermis;(3) the neuromast cupula changed from cylindrical to blade-shaped;(4) the free neuromasts went from occurring singly to being in clusters of a few single organs;(5) the arrangement changed from a linear array to no discemable pattern;(6) there was a significant increase in the number of free neuromasts after metamorphosis.In adult C.semilaevis,free neuromasts were only observed on the abocular side of the head.Thus,there were more free neuromasts located on the abocular side of the head with a higher concentration around the anterior nostril and mouth,which may have a mechanical sensory function to help locate food as an adaptation to a benthic mode of life.  相似文献   

17.
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