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1.
青藏高原土壤中链霉菌的分离鉴定及其抗菌活性研究 总被引：1，自引：0，他引：1
对从青藏高原采集的土壤样品中的链霉菌进行了分离鉴定,并对其抗菌活性进行了研究. 结果表明：11份土样中分离出菌落形态特征明显不同的链霉菌相似菌株151株,对其中43株进行了16S rDNA测序分析,41株菌株与已知链霉菌的相似性在99%以上,确定为链霉菌属（Streptomyces）菌种,而菌株WDL-DM-5和TTH-DM-1与已知链霉菌的序列相似性分别为90.76%和95%. 抗菌试验表明,68株链霉菌对革兰氏阳性菌和阴性菌有抗性,其中41株菌对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌都有明显拮抗性,表明青藏高原土壤中存在强拮抗性的链霉菌. 相似文献
2.
为探究湖北清江和尚洞岩壁放线菌物种组成及其与碳酸盐岩相互作用的关系,分别采集该洞穴无光区风化岩壁样品2份（W1和W2）和弱光区风化岩壁样品1份（W3）,采用经改良的5种已知放线菌分离培养基及1种自主设计的风化岩壁浸汁培养基两种策略优化设计分离培养基。采用稀释涂布法分离放线菌,并选择放线菌特异引物进行16S rRNA基因扩增和序列测定并构建系统发育树。结果表明,从3份风化岩壁样品中共纯化到放线菌72株,分属于放线菌纲（Actinobacteria）的4个属:链霉菌属（Streptomyces,69株）、红球菌属（Rhodococcus,1株）、冢村氏菌属（Tsukamurella,1株）和微杆菌属（Microbacterium,1株）,其中链霉菌属为优势菌属。无光区样品W1和W2分离到菌株较少,分别为9株和10株,而弱光区样品W3分离到菌株较多,为53株。研究结果为进一步研究放线菌与洞穴碳酸盐矿物相互作用的潜在关系奠定了基础。 相似文献
3.
本文报道来自西南印度洋超慢速洋脊49.6°E洋脊处的新鲜洋中脊玄武岩(MORB)玻璃样品的主微量元素和w(H2O)。样品w(H2O)范围为0.31%～0.40%，高于其他洋脊一般的N-MORB样品。样品H2O/Ce的比值范围为543～613，显著高于大西洋、太平洋MORB和前人发表的邻近洋脊样品数据。根据熔融模型计算，地幔源区w(H2O)高于450×10-6，显著高于前人估计的亏损地幔源区(DMM)w(H2O)范围50×10-6～200×10-6。流体活动性元素和Nb之间的线性正相关关系，以及w(H2O)与不相容元素之间的相关关系表明，相对高的w(H2O)和H2O/Ce比值并非遭受混染了的蚀变产物造成的。相反，它们反映了源区地幔的特殊性，很有可能来自于源区中小尺度的古老地幔楔熔融残余。 相似文献
4.
为了研究彬长矿区雅店煤矿的H2S异常和超限的形成机理,通过对矿井主采4号煤层地温条件、不饱和烃、硫酸盐分布、地下水pH值、硫酸盐还原菌(SRB)生存条件及采矿扰动等分析,认为研究区具有20～40℃适宜的地温条件和pH值为7.70～7.78的洛河组弱碱性水良好条件,为硫酸盐还原菌(SRB)苏醒和繁殖提供了生存条件,在硫酸盐还原菌(SRB)的还原作用下,使得煤层中的硫酸盐矿物被还原成为H2S代谢排出(生),进入煤层孔裂隙等储存空间(储),受煤系地层顶底良好的封存作用使得H2S得以保存,受采矿活动的影响,H2S沿裂隙和地下涌水逸散至巷道回风流中(运—逸),造成H2S局部异常和超限,构成了“生—储—保—运(移)—逸” H2S形成机理和条件。 相似文献
5.
UV/H2O2法降解乙酸影响因素的研究 总被引：3，自引：0，他引：3
通过采用UV/H2O2法,以乙酸为研究对象,高压汞灯为光源,在温度为（35±2）℃、曝空气量为0.8 L/min的条件下,研究了UV/H2O2技术对500 mL浓度为8 mmol/L的乙酸溶液进行的光降解反应。考察了各种因素对UV/H2O2降解乙酸的影响。实验表明,H2O2浓度为15 mmol/L、pH值为3.4时,乙酸降解效果最佳,此时,在240 min内,乙酸、TOC和COD的去除率可分别达到98%、98%和97%,体系中乙酸完全矿化。利用UV/H2O2法对实际糠醛废水进行了降解,效果良好。 相似文献
6.
冻土覆盖率高的小流域的径流形成受温度因素控制明显,普通水文模型不适用,而常规冻土水文模型因需要较多的气象观测要素而难以应用。考虑冻土流域产流机制,利用青藏高原腹地风火山小流域2017—2018年逐日降水、气温、径流观测数据,以降水、气温为输入,径流为输出,基于长短期记忆神经网络（LSTM）建立了适用于小流域尺度的冻土水文模型,并利用2019年观测数据进行验证。模型得益于LSTM特殊的细胞状态和门结构能够学习、反映活动层冻融过程和土壤含水量变化,具有一定的冻土水文学意义,能很好地模拟冻土区径流过程。模型训练期R2、NSE均为0.93,RMSE为0.63 m3·s-1,验证期R2、NSE分别为0.81、0.77,RMSE为0.69 m3·s-1。同时,为了验证模型可靠性,将模型应用于邻近的沱沱河流域,模型训练期（1990—2009年）R2、NSE均为0.73,验证期（2010—2019年）R2、NSE分别为0.66、0.64,模拟结果较好。考虑到未来气候变化,通过模型对风火山流域径流进行了预测：降水每增加10%,年径流增加约12%;气温每升高0.5 ℃,年径流增加约1%;春季融化期、秋季冻结期径流增幅明显,而由于蒸发加剧、活动层加深,径流在8月出现了减少。模型经训练后依靠降水、气温作为输入能较好地模拟、预测青藏高原冻土区小流域径流,为缺少土壤温度、水分等观测数据的冻土小流域径流研究提供了一种简单有效并具有一定物理意义的方法。 相似文献
7.
四川乌斯河铅锌矿床是赋存于震旦系灯影组白云岩中的大型铅锌矿床.本次研究结合最新的野外地质现象发现该矿床除存在前人所强调的沉积成矿作用以外,热液成矿作用非常明显.对该矿床流体包裹体进行细致的岩相学、显微测温和激光拉曼研究,揭示成矿流体特征,并探讨成矿机制.研究结果显示该矿床包裹体类型较为单一,以气液两相为主,均一温度主要集中于120~260 ℃,平均盐度为10.0% NaCl eqv,压力为32~68 MPa,成矿流体为中-低温、中等盐度.激光拉曼测试显示包裹体气相成分含有CH4、H2S、C2H6、C2H2、N2和NH3,为多元共存的流体体系.在热驱动力下(120~260 ℃),流体混合作用提供了物质基础(SO42-)、催化剂(Mg2+)和还原剂(有机质、CH4和H2S)促使硫酸盐热化学还原反应(TSR)启动.TSR反应过程中流体pH发生变化,进一步促进了金属硫化物沉淀. 相似文献
8.
冬克玛底冰川作为重要的长江源头之一,近年来主要以冰川气候、地质变化等自然地理方向的研究为主,而对于冬克玛底冰川不同生境中可培养细菌多样性及群落构成的研究还鲜有报道。为了阐明冬克玛底冰川可培养细菌多样性及其与环境因子的相关关系,发掘冰川微生物资源,针对冬克玛底冰川雪、冰和融水3种生境开展了研究。采用传统可培养法分离菌株,16S rRNA基因序列分析方法进行菌株鉴定,统计学方法分析可培养细菌多样性及其影响因素。结果表明,本研究中可培养细菌分属于放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)。其中放线菌门为优势菌门,库克菌属(Kocuria)、微杆菌属(Microbacterium)和马赛菌属(Massilia)为优势菌属。不同生境中的可培养细菌数量、群落结构和多样性均不同,冰样中可培养细菌数量最多、群落结构复杂并且多样性最高。冬克玛底冰川分离的36个菌属中有8个菌属未从其他冰川分离出。冰川不同生境中可培养细菌群落结构差异较大;Cl-和Ca2+ 相似文献
9.
日扎潜在巨型岩质滑坡位于四川降曲河左岸,多高山峡谷且河流纵坡降大,晚更新世以来强烈活动的金沙江断裂带东界断裂从斜坡坡脚通过。本文在遥感解译、现场调查和物探测试分析的基础上,对日扎滑坡的发育特征和形成机理进行了分析研究,认为日扎滑坡是在断裂活动、岩溶水、长期卸荷和重力作用下形成的一个潜在巨型深层岩质滑坡,在空间上可划分为后部拉裂变形区(Ⅰ)、中部挤压变形区(Ⅱ)和坡脚应力集中区(Ⅲ)等3个分区。目前日扎潜在巨型滑坡以后部蠕滑变形为主,在其后缘发育4条拉裂缝,物探推测最大裂缝宽度达30~35 m、深度达190 m。研究认为,日扎潜在巨型滑坡存在两种主要失稳模式:(1)高位剪出失稳,推测潜在失稳体积分别为(7.9~10.2)×107 m3(H1)、(2.3~2.9)×108 m3(H2)、(4.8~7.2)×108 m3(H3)和(6~10)×108 m3(H4);(2)深部蠕滑变形,在滑体深部存在蠕变带和锁固段,最大蠕动变形体厚度约300 m(H5)。日扎滑坡在长期卸荷与重力、地震和岩溶水等作用下容易造成锁固段力学强度弱化和失稳,发生高位启动-滑动-堵江灾害链,深层蠕变带容易对建设其中的深埋隧道等重大工程造成危害。该类潜在巨型岩质滑坡在青藏高原地区具有典型性,建议对日扎潜在巨型滑坡进行深入勘察,查明其空间结构特征与稳定性,必要时进行监测预警。 相似文献
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11.
The aim of this study was to assess the antimicrobial resistance and the prevalence of genes determining the presence of extended-spectrum beta-lactamase (ESBL) enzymes in Escherichia coli isolated from two major rivers of the Podhale region in southern Poland. In total, 196 E. coli isolates were analyzed—98 from each river—Bia?ka and Zakopianka, collected in 8 campaigns, over the period of two years. Antimicrobial resistance was assessed using disk diffusion method and PCR tests were conducted to detect the ESBL genes. In E. coli isolated from Bia?ka, the resistance to amoxicillin/clavulanic acid was detected most frequently (54.08%) and ESBL was detected in 14.29% of strains. In strains isolated from Zakopianka, most frequent resistance was observed toward ticarcillin (51.02%), while ESBL was observed in 16.33% of isolates. In the total pool of isolates, the resistance to amoxicillin/clavulanic acid was most frequent (48.98% of isolates) and ESBL producers comprised 15.30% of E. coli isolates derived from both rivers. Multidrug resistance was observed less frequently in strains derived from Bia?ka (4 isolates resistant to 10 and more antimicrobials) than from Zakopianka, where 10 isolates were resistant to 10 and more antibiotics. Out of the tested ESBL genes blaTEM was detected most frequently (45.4% of isolates), whereas blaCTX-M1 and blaCTX-M3 were recorded in one isolate. 相似文献
12.
双河洞放线菌多样性和抗菌活性的初步研究——以阴河洞和杉林洞为例 总被引：3，自引：3，他引：0
放线菌是微生物中重要的药用菌,其产生的抗生素是20世纪医学皇冠上最耀眼的明珠,但随着抗生素滥用,抗生素耐药现象日趋严重,因此从特殊生态环境中发现新放线菌物种和新抗生素克服耐药是科研人员正在尝试的途径之一,文章初步探究双河洞系统中阴河洞和杉林洞两个支洞的放线菌多样性,并对分离菌株的抗菌活性进行筛选。以纯培养方法获得放线菌,以16S rRNA基因序列比对,分析菌株类别,获得的菌株进行液体发酵,纸片琼脂扩散法测定其发酵产物对真菌和细菌的抗菌活性,同时用PCR技术进行NRPS、PKS I和PKS II抗生素生物合成基因的探测。从两个支洞样品中分离到的放线菌分布于7个科10个属,两个洞共66株放线菌中有32株的发酵产物在至少一个抗菌活性筛选中显示阳性,总阳性率为48.5%。抗生素生物合成基因的初步探测结果显示,31株菌存在至少一个生物合成基因,NRPS 28株,PKS I17株,PKS II 21株,其中12株同时具有3种生物合成基因。初步显示了双河洞系统蕴含丰富的放线菌资源,值得深入研究。 相似文献
13.
Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu 《地学前缘(英文版)》2012,3(3):327-334
Ice melt water from a 22.27 m ice core which was drilled from the East Rongbuk Glacier, Mt.Everest was incubation in two incubation ways:plate melt water directly and enrichment melt water prior plate,respectively.The abundance of cultivable bacteria ranged from 0-295 CFU mL-1 to 0-1720 CFU mL-1 in two incubations with a total of 1385 isolates obtained.Comparing to direct cultivation, enrichment cultivation recovered more bacteria.Pigment-producing bacteria accounted for an average of 84.9%of total isolates.Such high percentage suggested that pigment production may be an adaptive physiological feature for the bacteria in ice core to cope with strong ultraviolet radiation on the glacier.The abundances of cultivable bacteria and pigment-producing isolates varied synchronously along deplh:higher abundance in the middle and lower at the top and bottom.It indicated that the middle part of the ice core was hospitable for the microbial survival.Based on the physiological properties of the colonies,eighty-nine isolates were selected for phylogenetic analysis.Obtained 16S rRNA gene sequences fell into four groups:Firmicutes,Alpha-Proteobacteria,Gamma-Proteobacteria, and Actinobacteria,with the Firmicutes being dominant.Microbial compositions derived from direct and enrichment cultivations were not overlapped.We suggest that it is a better way to explore the cultiiiable microbial diversity in ice core by combining the approaches of both direct and enrichment cultivation. 相似文献
14.
F. Hosseini F. Malekzadeh N. Amirmozafari N. Ghaemi 《International Journal of Environmental Science and Technology》2007,4(1):127-132
Sodium dodecyl sulphate, (SDS) is an anionic surfactant that widely used all over the world. They will eventually end-up and accumulate in household or industrial sewage. Due to their high foaming capabilities, which can cause numerous problems in sewage treatment facilities as well as direct toxic effects on many different organisms in ecosystem; they are generally considered as serious pollutants. In this survey, two different bacteria were isolated from Tehran municipal activated sludge. Biochemical tests as well as 16S rRNA gene sequencing for identification have been applied. After experiments to optimize the pH and temperature for growth of the two bacterial isolates, the extent of SDS utilization was evaluated by HPLC method. Two bacterial isolates show which ability to rapidly and actively degrade SDS upon using it as their sole source of carbon. The identification tests have indicated the two isolates to be Acinetobacter johnsoni and Pseudomonas beteli. The Pseudomonas beteli and Acinetobacter johnsoni isolates were able to degrade 97.2% and 96.4% of the original SDS levels after 10 days of growth; respectively. Mixed culture of the two isolates did not significantly increase SDS utilization, (97.6%). In conclusion, the results of this study suggest that growth of simple bacteria such as Acinetobacter or Pseudomonas in household and industrial sewage can be cost-effective method anionic surfactants elimination. 相似文献
15.
Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery 总被引：1，自引：1，他引：0
S. Haghighat A. Akhavan Sepahy Ph.D. M. Mazaheri Assadi Ph.D. H. Pasdar Ph.D. 《International Journal of Environmental Science and Technology》2008,5(3):385-390
Microbially produced lipopeptide have been isolated and studied for microbial enhanced oil recovery. About 60 gram positive bacteria isolated from soil contaminated with crude oil, near the crude oil storage tank in Tehran Refinery, Tehran, Iran. However, most of these studies have produced lipopeptide by one of the pure-culture microbes isolated in a laboratory. Among the isolates, heamolytic tests revealed two biosurfactant producers. The isolated strains were designated as C2, E1. By using morphological, biochemical and molecular biology tests (16 SrRNA), the strains identified as Bacillus licheniformis and Bacillus subtitlis, respectively. Emulsification activity and measurement of surface tension indicated that, the isolates were high producers of biosurfactant. The product of C2 and E1 is mainly lipopeptide. This product reduce surface tension from 65 to 30 mN/m. Emulsified activity of crude oil was 92% for C2 and 90 % in case of E1. This is the first report of indigenous Bacillus licheniformis and Bacillus subtilis from a soil contaminated with oil in an Iranian refinery with ability to produce biosurfactant. 相似文献
16.
Variations in magnetostratigraphy, pedostratigraphy, grain size and magnetic susceptibility of the loess deposits near Beijing have been studied at two sections. The sections are about 400 km east of the main loess deposits in China, have a maximum thickness of 100 m and extend back to 1.1 Ma. The sequence consists of 14 loess–palaeosol couplets (S0‐S14), which correlate well with sequences in the Loess Plateau. Susceptibility records from the sites near Beijing are comparable to the Xifeng, Luochuan and Baoji sections located in the middle part of the Loess Plateau; however, the down‐core variations in the grain size in the Upper Lishi Formation exhibit some differences. The median grain size increases by about 25–30 µm from L4 to L2, with the sandy grains (>63 µm) increasing from 10–20 wt% to 40–50 wt% . This implies that the depositional environment of the dust sources in the Beijing loess section is different in some aspects from the Loess Plateau. The Beijing loess may have had a different dust source than the Loess Plateau. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
17.
Gaoyuan Li Hongchen Jiang Weiguo Hou Shang Wang Liuqin Huang Huilei Ren Shicai Deng Hailiang Dong 《地学前缘(英文版)》2012,3(3):317-325
The microbial diversity in Wuli Area,Qinghai-Tibetan Plateau was investigated using 16S rRNA gene phylogenetic analyses.A total of 117 bacterial and 66 archaeal 16S rRNA gene clones were obtained from the Wuli cold springs.The bacterial clones could be classified into Pwteobacteria,Acidobacteria, Deinococci,Sphingobacteria,Flavobacteria.Nitrospirae,Actinobacteria,Gemmatimonadetes, and unclassified-bacteria;and the archaeal clones could be classified into Crenarchaeota and Thaumarchaeota.Among the major groups,Pwteobacteria and Crenarchaeota were dominant in the bacterial and archaeal 16S rRNA gene clone libraries,respectively.The clone sequences obtained in Wuli cold springs were closely related to those from cold habitats,such as snow/ice/soils on high mountains or at high latitude.Especially,the microbial community composition of Wuli Area was more similar to that in Tibetan glaciers than cold environments of other locations.The data presented in this study have implications for a better understanding of microbial diversity in cold springs on the Qinghai-Tibetan Plateau. 相似文献
18.
S. Zaki Ph.D. S. Farag Ph.D. 《International Journal of Environmental Science and Technology》2010,7(3):553-560
The biosorption of copper (II) from aqueous solution using different bacterial strains was studied. Copper-biosorbing bacteria were isolated from tannery effluent collected from Borg Al-Arab, Alexandria, Egypt. These isolates displayed different degrees of copper biosorption under aerobic conditions. Based on 16S rDNA gene sequence analysis, three of them (S2, S5 and S7) were identified as Chryseobacterium sp., Enterobacter sp. and Stenotrophomonas sp., respectively. Initial copper (II) ion concentrations from 25–250 mg/L at constant temperature 30 °C were studied. The residual copper (II) concentration and its toxicity effect in solution were determined using atomic absorption spectrophotometer and bioluminescent bioreporter. The bioluminescence inhibition of strain (S5) reached to 91.4 % as compared with the strain (S7) reached to 83.3 % at 225 mg/L of copper ion where the maximum biosorption efficiency for S5 and S7 were 71 % and 70.1 % correspondingly using atomic absorption. The bioluminescent bioreporter was proved to be fast and accurate technique for measurement the toxicity effect of residual copper (II) in solution. 相似文献
19.
Sheng Yu Peng Zou Wei Zhu Lin Xiao Aijun Miao Lijuan Jiang Xiaolin Wang Jun Wu Liuyan Yang 《Environmental Earth Sciences》2013,69(5):1523-1528
The effects of organic matter, humic acid and Tween-80 on decabromodiphenyl ether (BDE-209) behavior in soil columns were investigated. The BDE-209 transport was simulated in 4-cm-length soil columns whether organic matter was added or not. A high concentration of BDE-209 was washed out of the soil column in the presence of 500 mg L?1 of Tween-80 for forming and suspending contaminated soil colloids in more than 4-cm-length ones (especially in 10-cm-length ones). While the humic acid was to facilitate BDE-209 adsorption onto soil particles (like soil colloids), Tween-80 was to enhance BDE-209 movement in porous media. The significant concentration averaged from 0.2 to 0.1 μg L?1 in soil columns of length from 10 to 24 cm with Tween-80 addition by comparing the estimated marginal means (p < 0.05, SPSS). Contrasted with humic acid-binding BDE-209 in soil particles, Tween-80 could carry contaminant soil colloids into deeper layers and even affect the final effluents of 25-cm-length columns. It was visibly presented that the BDE-209 concentration in the effluents was mainly induced by Tween-80. Thus, BDE-209 was carried by soil colloids to transport and pollute longer and wider soil distance with the help of the effective promoters and stabilizers of Tween-80 and humic acid in soil matrix. 相似文献