Acute Toxicity of Phenol,Pentachlorophenol and Sodium Pentachlorophenate to a Freshwater Ostracod Cypris subglobosa (Sowerby)     

P. S. Rao  V. S. Durve  B. S. Khangarot  S. S. Shekhawat 《洁净——土壤、空气、水》1983,11(4):457-465

The toxicity of phenol, pentachlorophenol and Na-pentachlorophenate to Cypris subglobosa is determined in the batch experiment for 96. h. In the sequence mentioned, the LC_50,96h of the three substances are 71.8, 6.56 and 4.41 mg/l, respectively. In the range of 6 … 95 h the mean survival time shows a negative regression to the toxicant concentration, which, however, is not significant statistically for Na-pentachlorophenate. In agreement with this, the LC₅₀ for 12, 24, 48, 72 and 96 h of exposure shows a significant decrease. Due to the comparatively high LC_50,96h for pentachlorophenol and Na-pentachlorophenate for C. subglobosa about in comparison with fish, the species is little suited to the toxicological investigation of wastewater.  相似文献   

Short Term Effects of Aldrin and Swascofix CD-38 on the Feeding and Respiratory Behaviours of Clarias batrachus and Cirrhina mrigala     

A. K. Gupta  S. S. Dhillon  I. P. Tonk  S. R. Verma 《洁净——土壤、空气、水》1983,11(4):423-432

Fish were kept in non-toxic water or at 0.5, 0.25 and 0.125 of the LC_50,96h of the ten-side or insecticide for 24 h and fed with toxified and non-toxified feed. Toxified feed in normal water resulted in a maximum feed intake, while normal feed in toxified water resulted in a minimum one. In each case, the feed intake was lower compared with the control, and the feed intake was inversely proportional to the toxicant concentration. In a simple respirometer the oxygen consumption in mg · kg^?1 · h^?1 was tested for 1, 2, 4, 6, 8, 10, 12, 24, 36, 48, 60, 72, 84 and 96 h of exposure to 1.5, 1.0 and 0.5 of the LC_50,96h of both substances. It was a flow device which was closed only for measurement for one hour. 1.5 LC₅₀ Aldrin causes a continuous reduction of respiration until death. The same effect occurred with Cl. batrachus for 1.5 LC₅₀ of the tenside, whereas with C. mrigala death was caused by a pronounced increase of respiration. In all other cases, after a phase of sensitization for 4 … 8 h respiration increased considerably to more than 300% of the control, which was followed after 12 … 24 h by acclimatization to normal respiration values.  相似文献   

Acute Toxicity of Distillery Effluent on a Freshwater Fish and Three Pond Snails     

B. S. Khangarot  P. S. Rao  S. S. Shekhawat  V. S. Durve 《洁净——土壤、空气、水》1985,13(1):53-58

The LC₅₀ for 12… 96 h of a distillery waste water were determined for Lebistes reticulatus, Lymnaea luteola, Lymnaea acuminata and Viviparus bengalensis in the batch experiment with daily exchange of the medium. The sensitivity of the species increases in the given sequence with values of the LC_{50,96 h} of 10.77 to 3.72 % proportion of wastewater in the medium. Compared with this, with values of the LC_{50,48 h} of 14.06… 15.71 % after 48 h of exposure there is no significant difference in sensitivity, whereas after exposure for 12 and 24 h the sensitivity grows in the following order: V. bengalensis, L. acuminata, L. luteola, Lebistes reticulatus. Toxicity is obviously primarily determined by the sulphides; with increasing time of exposure also the suspended matter and other factors act toxically.  相似文献   

Comparative Toxicity of Heavy Metals and Interaction of Metals on a Freshwater Pulmonate Snail Lymnaea acuminata (Lamarck)     

B. S. Khangarot  S. Mathur  V. S. Durve 《洁净——土壤、空气、水》1982,10(4):367-375

In static bioassays the toxicity of heavy metal ions against Lymnaea acuminata over 24 … 96 h is tested. The values of the LC_50,96h for the metals tested are, in mg/l: Hg²⁺ ?0.023; Cu²⁺-0.034, Cd²⁺ ?0.872, Ni²⁺ ?2.78, Cr⁶⁺ ?5.97 and Zn²⁺ ?10.49. In the combined solutions of Cu²⁺, Ni²⁺ and Zn²⁺ at least an additive effect of toxicity occurs in the presence of two metals. The same holds for the presence of the three metals; in this case, the relative toxicity against the mixture of two metallic salt solutions appears to be slightly reduced. The acute manifestation of the toxic effect occurs within 48 h, subsequently the relative mortality decreases especially in the mixed solutions.  相似文献   

Toxicity of the Herbicide 2.4-D-Na to Two Species of Freshwater Prawn of the Genus Macrobrachium     

Omkar  G. S. Shukla 《洁净——土壤、空气、水》1984,12(3):285-289

In batch experiments with exposure for 24 to 96 h Macrobrachium naso and M. dayanum were exposed to solutions of the sodium salt of 2,4-dichlorophenoxy acetic acid. The LC_50,96h are 2.397 g/l for Macrobrachium naso and 2.275 g/l for M. dayanum. No mortality was observed in solutions of 2.1 and 1.85 g/l. The differences in toxicity are not dependent on the size of the organisms, but they are species-specific.  相似文献   

“Man and Biosphere” — Studies on Sikkim Himalayas. Part 2: Acute Toxity of Mixed Copper-Zinc Solutions on Common Carp,Cyprinus carpio (LINN.)     

B. S. Khangarot  A. Sehgal  M. K. Bhasin 《洁净——土壤、空气、水》1984,12(2):131-135

In batch experiments for 6 to 48 h, individuals of Cyprinus carpio (2 g individual weight) were exposed to mixed solutions of copper and zinc sulphate: pH = 6.3, temperature 15 °C, 6.8 mg/l O₂, 7.1 mg/l Ca²⁺, 0.7 mg/l Mg²⁺. The LC₅₀ for the individual substances on exposures of 24 and 48 h amount to 9.04 and 7.28 mg/l, resp., of Zn and 0.11 and 0.095 mg/l, resp., of Cu. The sum of the biological activity of the two kinds of metal ions is assessed according to MARKING'S index: at low concentrations of the mixed solutions the two metals show an additive effect, at higher concentrations a synergistic effect occurs.  相似文献   

Effect of Zinc,Copper and Mercury on Channa marulius (HAMILTON)     

B. S. Khangarot 《洁净——土壤、空气、水》1981,9(6):639-649

Static bioassay acute toxicity tests of Zinc, Copper and Mercury were conducted to determine the median lethal concentrations (LC₅₀s) of a freshwater teleost Channa marulius (HAM .) The 96 h LC₅₀ and 95% confidence limits for Zn²⁺ were 25.61 (24.13 … 27.12) mg/l; 0.90 (0.80 … 1.038) mg/l; for Cu²⁺ and 0.314 (0.257 … 0.371) mg/l for Hg²⁺. However, these values decreased at 240 h of exposure and were: 21.09 (18.29 … 24.60) mg Zn²⁺/l; 0.66 (0.568 … 0.841) mg Cu²⁺/l; and 0.131 (0.103 … 0.158) mg Hg²⁺/l. The relative potency ratio of Zn/Hg, Zn/Cu and Cu/Hg suggests that fish were most sensitive to Hg, followed by Cu and Zn ions. The acute toxicities of mixtures of Zn²⁺?Cu²⁺; Zn²⁺?Hg²⁺, Cu²⁺?Hg²⁺ and Zn²⁺?Cu²⁺?Hg²⁺ up to 48 h of exposure were also investigated. The additive index and ranges for Zn²⁺?Cu²⁺ were ?0.241 (-0.577 … 0.054); 0.056 (-0.269 … 0.475) for Zn-Hg; 0.285 (-0.043 … 0.718) for Cu-Hg; and -0.542 (-1.215 … 0.005) for Zn-Cu-Hg. All the mixtures tested showed a greater than additive toxicity because index ranges overlapped zero.  相似文献   

Toxicity of Interactions of Zinc —Nickel,Copper —Nickel and Zinc —Nickel —Copper to a Freshwater Teleost,Lebistes reticulatus (PETERS)1     

B. S. Khangarot 《洁净——土壤、空气、水》1981,9(5):495-503

The lethal toxicity of mixtures of Zn²⁺ —Ni²⁺, Cu²⁺ —Ni²⁺ and Zn²⁺ —Cu²⁺ —Ni²⁺ to common guppy at 21£C in hard water (total hardness = 260 mg/l as CaCO₃) was studied under static bioassays test conditions with renewal of the test solutions every 24 h. The heavy metals were tested separately and in mixtures. The 48 h median lethal concentrations (LC₅₀) for individual salts were 75 mg/l Zn²⁺, 37 mg/l for Ni²⁺ and 2.5 mg/l for Cu²⁺. Concentrations were expressed in “toxic units” by taking them as proportions of LC₅₀ values. Experiments showed that in the Zn²⁺-Ni²⁺ mixture, when Ni²⁺ was more in proportion, the toxicity was more than additive. The 48 h LC₅₀ value and 95% confidence limits in the Ni²⁺-Cu²⁺ mixture were 0.684 (0.484 … 0.807) toxic units and the mixture produced more than the additive toxicity (synergism.). The LC₅₀ value and its 95% confidence limits in a Zn²⁺?Cu²⁺?Ni²⁺ mixture also suggested that the mixture was again strictly additive. The results indicate that heavy metallic mixtures would pose a greater toxicological danger to fish than the respective individual metals.  相似文献   

Studies on the Toxicity of Some Insecticides to a Freshwater Teleost Lebistes reticulatus (PETERS)     

P. K. Gupta  V. S. Mujumdar  P. S. Rao 《洁净——土壤、空气、水》1984,12(6):629-636

In batch tests with daily exchange of the medium the toxicity of endosulfan, lindane, aldrin, chlordane, rogor and novocron is investigated during times of exposure of 12… 96 h. The mean survival periods and the LC₅₀ are determined. Toxicity increases in the following sequence (LC_50,96h) (in mg/1): novocron (22.65), rogor (18.97), chlordane (0.341), aldrin (0.017), lindane (0.0160), endosulfan (0.0027). The following safe concentrations in mg/1 are recommended: 0.0006 endosulfan, 0.0041 lindane, 0.0010 aldrin, 0.1436 chlordane, 5.4086 rogor and 7.2309 novocron.  相似文献   

Zytotoxikologische Untersuchung von Schadstoffen und Wasserblüten mit gefrierkonserviertem Bullensperma (Spermatest)     

L. Kalbe  R.-D. Recknagel 《洁净——土壤、空气、水》1985,13(2):185-194

The method is based on the determination of the number of the nonmotile sperms and/or the lethally damaged sperms with the aid of the fluorescent dye primuline. Incubation takes place at a suspension density of 10.000/mm³… 38.000/mm³, at 40°C or 46 °C for 15… 60 min. During the test with sublimate the loss of motility amounts to 55% at 4 mg/l Hg²⁺, the LC₅₀ is 7.3 mg/l Hg²⁺. Phenyl-mercuric acetate causes the total loss of motility with 20 mg/l, the LC₅₀ is above 168 mg/l (equivalent to 100 mg/l Hg²⁺). The LC₅₀ of sodiumpentadecylmonosulphonate and dodecylpyridinchloride are 11.6 and 15.8 mg/l, resp. Filtrates of blooming of cyanophyceae on Baltic, having been digested by repeated freezing and thawing, with 1.5… 6.4 g/l dry matter showed motility losses of 30… 100 % for Nodularia spumigena, whereas no significant effect was produced by a filtrate of Microcystis aeruginosa with 28.2 g/l dry matter as well as water-blooming of Nodularia spumigena from the Small Jasmund Bodden.  相似文献   

Toxicity and long term effects of a sublethal concentration of cadmium on the growth of the fingerlings of Ophiocephalus punctatus (BL.)     

J. P. Shukla  K. Pandey 《洁净——土壤、空气、水》1988,16(5):537-540

The LC₅₀ for exposure of 48 and 96 h is 34.64 and 18.5 mg/l Cd²⁺, respectively. During the exposure to 3.5 mg/l Cd²⁺ from the 13th day significant differences of length and mass occur with respect to the control. Over a total of 31 days of experiment the reduction of growth rates is 11% for the longitudinal growth and 16% for the increase in weight.  相似文献   

Aldrin Induced Changes in the Activities of Three Phosphatases of Macrobrachium lamarrei (H. Milne EDWARDS)     

Omkar  R. Murti  G. S. Shukla 《洁净——土壤、空气、水》1984,12(6):609-613

Macrobrachium lamarrei is exposed to 0.008, 0.017 and 0.021 mg/1 aldrin (0.4, 0.8 and 1.0 of the LC_50,96h) for 24… 96 h and after exposure the activities of the acid, alkaline and glucose-6-phosphatases are determined. With concentration and time of exposure the acid phosphatase shows an increasing activity up to 170 % compared with the control, which is interpreted as a part of prenecrotic changes in the cells. With concentration and time of exposure the alkaline phosphatase shows a decreasing activity down to 20 % of the control, which is indicative of considerable disturbances of the total metabolism. The glucose-6-phosphatase shows an increasing activity with the aldrin concentration up to 150 % during 72 h; then the activity drops to 60… 80 %. The initial rise in the glucose concentration of blood caused by that is the clear consequence of a stress reaction and the change of metabolism resulting from it.  相似文献   

Efficaciousness of Certain Chemicals to Control Dracunculiasis — A Field Trial     

S. Mehta  R. C. Srivastava 《洁净——土壤、空气、水》1984,12(3):291-300

To prevent infection with Dracunculus medinensis, a carrier host, the copepod Cyclops, has to be controlled. After preceding laboratory investigations a field trial with well water and different additions of chlorine and sodium permanganate is carried out. The exposure is performed at an ambient temperature of 25… 40 °C and in bright and dark samples (5… 11 h of sunshine/d) as well as in the well. The residual concentrations of the two chemicals are an exponential function of the time of exposure with half-times between 6 and 23 h for chlorine and 11… 30 h for sodium permanganate. The depletion of the two oxidizing agents under light is faster than in darkness. Dosage should be done in such a way that the LC_90,24h after 24 h of exposure will not be fallen below; in a specific case, this guarantees at the same time that the permissible concentrations for drinking water will not be reached 96 h after addition. For this, initial concentrations of 30 mg 1 Cl₂ or 50 mg/l KMnO_r are necessary. Under these conditions, the Cyclops population had died on the seventh day after the application of chemicals, but after seven weeks it reached the original density again. With one application of chloride or sodium permanganate a week Dracunculiasis can be completely controlled.  相似文献   

The acute toxicity of silver to some freshwater fishes     

B. S. Khangarot  P. K. Ray 《洁净——土壤、空气、水》1988,16(5):541-545

In the batch experiment with daily exchange of medium the acute toxicity of Ag from AgNO₃ to Channa punctatus, Lebistes reticulatus and Puntius sophore is determined over 12 to 96 h exposure. In general, the sensitivity of species increases in the order mentioned. The LC_{50, 96h} are 18.89 μg/l Ag for Channa punctatus, 7.55 μg/l Ag for Puntius sophore and 6.44 μg/l for Lebistes reticulatus. For 12 and 48 h exposure the values in the same order of species are: 62 and 30, 44 and 14 as well as 57 and 16 μg/l Ag.  相似文献   

Effect of Complexing Agents on the Acute Toxicity of Copper to Common Guppy Lebistes reticulatus (PETERS)     

B. S. Khangarot 《洁净——土壤、空气、水》1981,9(5):505-509

The acute toxicity of copper and copper plus complexing agents to common guppy Lebistes reticulatus was studied for 96 h by a static bioassay technique. The addition of complexing agents viz. disodium salt of EDTA, citric acid, sodium thiosulphate and glycine in Cu²⁺ solutions caused a great decrease in the per-cent mortality as compared to that of Cu²⁺ test solutions alone. 10 mg/l of complexing agent was added in each copper concentration in all the test series. The 96 h LC₅₀ values and 95% confidence limits in mg/l of Cu²⁺ plus were 1.23 (0.95…1.65) for Cu²⁺ alone; 4.30 (4.04 … 4.55) for Cu²⁺ plus EDTA; 1.94 (1.69 … 2.18) for Cu²⁺ plus citric acid; 3.44 (2.96 … 3.74) for Cu²⁺ sodium thiosulphate and 2.29 (2.22 … 3.02) for Cu²⁺ plus glycine.  相似文献   

Acute and Chronic Toxic Effects of Cadmium on some Haematological,Biochemical, and Enzymological Parameters in the Fresh Water Teleost Fish Channa punctatus Akute und chronisch toxische Wirkungen von Cadmium auf einige hämatologische,biochemische und enzymologische Parameter im Süßwasserfisch Channa punctatus     

K. V. Sastry  V. Shukla 《洁净——土壤、空气、水》1994,22(4):171-176

The haematological, biochemical, and enzymological alterations produced on exposure of Channa punctatus to LC₅₀ (11.2 mg/L) for 96 h and to a sublethal concentration (1.12 mg/L) of cadmium (Cd²⁺) for 15, 30, 60, and 120 days have been studied. The fish were hypoglycemic and hypolactemic. The pyruvate content of blood and liver decreased in acute and all stages of chronic exposure except for 30 days where significant increase was recorded. Depletion was noted in the total protein and glycogen content of liver and muscle, and the level of lactate in liver in the two types of exposure. The levels of muscle lactate and pyruvate increased in both exposures. The activities of hexokinase, glucose-6-phosphatase, and lactate dehydrogenase decreased in liver after 96 h and all stages of chronic exposure except 30 days. The activities of hexokinase and lactate dehydrogenase in muscle and glutamate-oxalacetate transaminase and glutamate-pyruvate transaminase in serum, liver, and muscles increased in all exposures. The activities of succinate and malate dehydrogenases in liver and muscles decreased after acute and all stages of chronic exposure. The above results suggest that glycolysis and gluconeogenesis were impaired in liver but glycolysis prevailed in muscle. Aerobic oxidation is adversely affected in metal exposed fish, while proteolysis was increased.  相似文献   

Survivality and Impaired Growth in Arsenic Treated Fingerlings of Channa punctatus,a Fresh Water Murrel     

J. P. Shukla  K. N. Shukla  U. N. Dwivedi 《洁净——土壤、空气、水》1987,15(3):307-311

The LC₅₀-values of As³⁺-compounds for Channa punctatus are determined for a 48-h exposure to 19.4 (18.02… 21.3) mg/1 and for a 96-h exposure to 14.3 (13.06… 16.80) mg/1. At a long-time exposure to 7.0 mg/1 As³⁺, during the first seven days there does not occur any change of growth, then the growth in length and the increase in weight decreased by 6.6 and 11.7%, respectively, till the 31st day. At the same time, the RNA content of the muscle decreased by 11.5% to 966.7 μg/g fresh weight and the protein content decreased by 12.5 % to 95.39 mg/g fresh weight.  相似文献   

Effect of lethal concentrations of copper on Mystus bleekeri (day)     

B. S. Khangarot  P. K. Ray 《洁净——土壤、空气、水》1988,16(5):547-550

In the batch test with daily exchange of medium the effect of copper ions at 2.4 mmol/l (Ca²⁺ + Mg²⁺) over 10 d of exposure is investigated. The LT₅₀ values and 95% confidence limits at 3.7, 2.1 and 1.0 ppm of Cu were 26.7 (23.6… 29.9), 47.5 (39.6… 57.0) and 101.5 (78.5… 130.7) h, respectively. The LC₅₀ values of copper in ppm were 4.05 (24 h), 1.69 (48 h), 0.829 (9.96 and 0.776 (240 h).  相似文献   

Deleterious effects of Malathion on survivality and growth of the fingerlings of Channa punctatus (BLOCH.), a freshwater murrel     

J. P. Shukla  M. Banerjee  K. Pandey 《洁净——土壤、空气、水》1987,15(6):653-657

The LC₅₀ of Malathion for Channu punctatus is determined for 24… 96 h of exposure as 10.95… 3.22 mg/l. At exposure to 2.5 mg./l Malathion, growth–measured by increase in weight–is significantly reduced from the 19th day and reduced by 10 % till the 31st day. During the same period of time, the RNA and protein contents of the muscle is lowered by 10 and 15 %, respectively, as compared with the control. In addition to an acute toxic effect, in sublethal concentrations Malathion has also pronounced effects on growth and metabolism.  相似文献   

A Study on the Effect of Temperature upon the Toxicity of Sodium Pentachlorophenate to the Freshwater Snail Viviparus bengalensis L.     

P. K. Gupta  V. S. Durve 《洁净——土壤、空气、水》1984,12(4):369-375

The toxicity of Na-pentachlorophenate to Viviparus bengalensis is investigated by batch tests with daily exchange of medium in the temperature range between 19 °C (February), 27 °C (April), 32 °C (June) and 30 °C (August) at times of exposure between 12 and 96 h. In the range of higher concentrations, pronounced abnormalities in behaviour by secreting mucus and discharging eggs and embryos occur. The LC₅₀ are lowest for all times of exposure at 27 °C, whereas the times of survival with a given concentration are highest at 30 °C. From the LC_{50, 96h, 27°C} = 66 μg/l results a safe concentration of only 47 μg/l Na-pentachlorophenate for Viviparus bengalensis.  相似文献