共查询到17条相似文献，搜索用时 125 毫秒
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采用FISH技术对2008年冬、夏两季太湖梅梁湾水体中细菌优势种群的丰度进行了对比分析。实验中,选取荧光素标记的特异寡核苷酸探针,与太湖梅梁湾水体中的四类细菌优势种群α-变形菌(α-proteobacteria),β-变形菌(β-proteobacteria),γ-变形菌(γ-proteobacteria)和拟杆菌(Bacteroidetes)进行原位杂交。结果表明:①所测定的四类细菌优势种群占总细菌的比例在冬季均显著高于夏季(P0.01);②水体中α-proteobacteria的丰度随TP浓度的增加而显著增加(R~2=0.860,P0.01);③随着Chl.a浓度的增加,水体中细菌的丰度呈上升的趋势,尤其是其中的γ-proteobacteria,其增大趋势更加显著(R~2=0.857,P0.01);④细菌种群的丰度受水体中PO_4~(3-)浓度的影响,尤其是对Bacteroidetes的影响更加显著(R~2=0.733,P0.05)。 相似文献
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改良型生物稳定塘对滇池流域受污染河流净化效果 总被引：5，自引：1，他引：4
对滇池流域大清河生物稳定塘系统中的水质净化效果进行了分析,同时对各塘中的浮游藻类种类组成、细胞密度和多样性进行了调查。结果表明,当污水流经预处理塘、好氧塘、水生植物塘、养殖塘的过程中,pH、DO、叶绿素a浓度呈逐渐上升趋势,TN、TP、NH_4~+-N、BOD_5和COD浓度呈逐渐下降趋势,生物稳定塘系统对TN、TP、NH_4~+-N、BOD_5和COD的去除率分别达29.29%,48.68%,33.68%,68.14%和71.25%。叶绿素a浓度和pH(r=0.955,P0.05)、DO(r=0.992,P0.01)显著正相关,而和TN(r=-0.936,P0.05)、TP(r=-0.925,P0.05)以及NH_4~+-N(r=-0.927,P0.05)等显著负相关。在塘系统中,共出现浮游藻类53种,藻类种类数和生物多样性呈增加趋势,总细胞密度呈下降趋势;塘系统中共出现6种藻类优势种,其中绿色微囊藻和惠氏微囊藻细胞密度呈现出逐渐下降的趋势,梅尼小环藻、啮蚀隐藻、美丽网球藻和球囊藻细胞密度呈现出逐渐上升的趋势。 相似文献
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兴凯湖春季水体悬浮颗粒物和CDOM吸收特性 总被引：1，自引：0，他引：1
为了分析兴凯湖水体光学活性物质的吸收特性、来源和空间分布以及对400~700 nm范围内总吸收的贡献,于2013年5月对该水体进行野外实验,对水体中浮游藻类、非藻类颗粒物和有色可溶性有机物的吸收特性和水质参数进行测定.结果表明:总悬浮颗粒物的吸收光谱与非藻类颗粒物相似,色素颗粒物含量较少且单一,非藻类颗粒物在总悬浮颗粒物吸收中占主导地位,其贡献率始终在50%以上.CDOM吸收曲线的拟合函数斜率值Sg均高于其它水体.440 nm处总悬浮颗粒物和非藻类颗粒物的吸收系数ap(440)、ad(440)与总悬浮颗粒物、无机悬浮颗粒物和有机悬浮颗粒物浓度相关性均较好,与叶绿素a(Chl.a)浓度的相关性较差.兴凯湖与其它Ⅱ类水体的差异性表现在440 nm处CDOM吸收系数ag(440)与Chl.a浓度、溶解性有机碳(DOC)浓度均无显著相关性,说明DOC以无色部分为主.总体上,大兴凯湖各吸收系数和水质参数均值均低于小兴凯湖,后者水质受农耕区退水及周围渔业、旅游业的影响较大. 相似文献
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为探讨非生物因子和生物因子对水体叶绿素a(Chl.a)浓度变动的相对重要性,对芜湖市内的汀棠湖(每半月1次)和镜湖(每4天或7天1次)进行高频采样,分析理化因子及浮游动物密度与Chl.a浓度间的关系.结果表明,从全年来看,2个湖泊的Chl.a浓度均与水温和透明度间分别呈现显著的正相关性和负相关性,与浮游动物密度均无显著相关性;镜湖Chl.a浓度随着总磷浓度和氮磷比的增加分别呈上升和降低趋势.从季节来看,冬季两湖中的Chl.a浓度最低,轮虫密度与此阶段镜湖中藻类Chl.a总浓度和小型藻类的Chl.a浓度间均呈显著负相关,且其对上述两类Chl.a浓度变动的影响程度最大,表明轮虫对藻类的牧食效应降低了冬季镜湖中的Chl.a浓度.从各环境因子对Chl.a浓度变动影响的相对权重来看,除了冬季的镜湖外,2个湖泊中的两类Chl.a浓度均与透明度的关系最密切,且均随着透明度的上升而降低,暗示了透明度可作为预测小型封闭性浅水湖泊中Chl.a浓度的简便指标. 相似文献
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氮、磷等营养物质对低洼盐碱地鱼塘水质的影响 总被引：4，自引：0，他引：4
通过在实验围隔中添加不同浓度的氮、磷等营养物质，对黄淮海平原低洼盐碱地鱼塘中的水质变化情况进行了初步研究，结果表明：1）围隔水体中异养细菌数量的变动，与水体中氮、磷等营养物质的浓度及比例密切相关，尤其是水体中磷的浓度对异养细菌数量的影响更为显著。2）围隔水体中的氮、磷等营养浓度及比率，明显地影响着其中藻类的数量和生物量。在实验条件下，水体中磷对藻类生长的影响要大于氮对藻类生长的影响，水体中N：P比为10：1左右是比较适宜藻类生长的比例。3）围隔水体中氮、磷等营养盐的去除能力与其中藻类的生物量及水体中N：P的比率密切相关，当围隔水体中的N：P比为10：1左右时，围隔水体中的藻类生长最好，生物量增加最快，相应地其对TN、TP的去除率也是最高的。 相似文献
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三峡澎溪河高阳平湖高水位时碱性磷酸酶活性及其动力学特征 总被引：3，自引：2，他引：1
三峡水库汛末蓄水后易出现支流回水区磷累积现象,并在冬季末期常出现硅藻水华现象.为研究汛末蓄水的磷积累与冬季末期硅藻水华的相互关系,分析2013年1 3月三峡澎溪河高阳平湖库湾水体中碱性磷酸酶活性、磷形态的转化和藻类生长的协同过程.结果表明,总碱性磷酸酶活性(TAPA)及其最大反应速率(Vmax)、特异性碱性磷酸酶活性(PAPA/Chl.a)及PAPA与TAPA的比值(PAPA/TAPA)随着时间推移总体呈先增加后减小而后再增加减小的双峰趋势,分别在2月中旬和3月中旬达到峰值.根据冬季末期水华暴发程的特点将其分为Ⅰ、Ⅱ、Ⅲ、Ⅳ4个阶段.Ⅰ阶段为诱导期,水体活性磷主要来自藻类碱性磷酸酶分解的有机磷,藻类对磷过度摄取.Ⅱ阶段为过渡期,温度低,水体碱性磷酸酶活性相对较低.Ⅲ阶段为水华时期,水中碱性磷酸酶主要来源于细菌,叶绿素a浓度达到最大,溶解态反应性磷浓度达到最低;Ⅳ阶段为水华末期,水体叶绿素a浓度逐渐下降,溶解态反应性磷浓度回升,水中碱性磷酸酶主要来源于细菌. 相似文献
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水体富营养化极易引起湖泊水库如藻类水华等水生态系统环境问题。氮素作为初级生产力的限制性生源要素之一,认识其在水华形成过程中潜在作用至关重要。本研究选取胶东半岛低碳高氮水库水体进行模拟实验,通过添加不同剂量硝态氮,探究高硝态氮输入对库区水体藻类和细菌群落结构的影响。结果表明:(1)当硝态氮作为唯一氮源,随着培养时间延长,硝态氮浓度显著下降,亚硝态氮和氨氮浓度逐渐升高,表明微藻和细菌共同作用可能将硝态氮转化为亚硝态氮和氨氮;(2)当硝态氮浓度为6 mg/L时,藻类叶绿素a浓度达到最高值,随着硝态氮浓度升高,叶绿素a浓度则会降低;(3)添加硝态氮后,蓝藻门成为优势藻类,绿藻门次之;变形菌门相对丰度显著升高。研究结果为低碳高氮类水体暴发蓝绿藻水华及有效防控提供理论依据和技术支撑。 相似文献
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江西柘林水库是长江中游的大型峡谷型旅游性水库,库容79.2×10~8m~3,长115 km.2015年4月在56 km的中心库区走航和定点测量了水库表层水体浮游藻类、叶绿素a(Chl.a)浓度与溶解无机氮(DIN)浓度、溶解无机磷(DIP)浓度、溶解硅(DSi)浓度、水温、浊度和溶解氧浓度等环境因子的分布特征.结果表明:1)水库属于中营养水体,表层主要浮游藻类(细胞丰度1000 cells/L)有34种,平均生物量为0.41 mg/L.主要优势藻类(优势度≥0.02)为硅藻和蓝藻,藻类组成与DIN浓度、DIP浓度、DSi浓度和水温等环境因子关系密切,4种因子对藻类结构的解释水平达60%以上.2)水库水体Chl.a浓度具有显著的次表层叶绿素最大值(SCM)现象,SCM层深度为3~8 m,厚度为2~7 m,SCM层占整个水体的25.2%~74.1%.SCM层的藻类对营养盐吸收消耗致使DIN、DIP和DSi浓度下降,同时藻类的产氧使溶解氧浓度增加.3)水库对DSi具有显著的生物过滤器效应,中、上层约有11%~12%的DSi被生物吸收利用,从上游至下游,累积约有21%的DSi被藻类吸收沉降于库底.4)人类氮、磷排放对水库生态和水质有严重影响,毗邻县城区域水体的Chl.a和DIP浓度分别是自然河段的2.9倍和3倍左右. 相似文献
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混合藻类高光谱特征及其叶绿素a分离定量模型 总被引：1，自引：1，他引：0
利用地物高光谱遥感技术,在室内进行小球藻、聚球藻及其混合藻高光谱测量.得到这3组藻的反射光谱特征,同时进行叶绿素a(Chl.a)浓度测量.利用Matlab软件中的神经网络工具箱对得到的高光谱数据进行了曲线拟合.并用拟合结果和光谱测量实际结果分别建立了两种单一藻类的定量模型单一小球藻Chl.a最优的定量模型为用反射率实际值建立的小球藻单波段反射率模型Chl.a=1×10~7(R_(687))~2-37016R_(687)+53.64.单一聚球藻Chl.a最优的定量模型为利用反射率实际值建立的聚球藻两波段模型Chl.a=853 15×[R~(-1)(669)-R~(-1)(730)]×R(730)+505.78.在对两种单一藻类定量模型研究的基础上分别用单波段反射率分离模型、三波段分离模型和两波段分离模型对由小球藻和聚球藻组成的混合藻进行了Chl.a浓度分离.其中单波段反射率分离模型和两波段分离模型得到了较好的分离结果,单波段反射率分离模型结果要优于两波段分离模型结果.利用神经网络模型拟合值构建的模型要优于直接用反射率测量值构建的模型,而三波段分离模型的分离结果不理想,不适用于本研究. 相似文献
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运用水下饱和脉冲荧光仪(Diving-PAM)测定附着了水网藻的亚洲苦草(Vallisneria asiatica)叶片的荧光参数和快速光响应曲线.在水网藻主要附着的苦草叶片上部,15 d后Fv/Fm显著下降,光系统(PS)的光化学效率显著降低;附着水网藻的叶片通过热的形式耗散掉的能量极显著增加(P＜0.01),有效荧光产量、光化学淬灭系数和相对光合电子传递速率(rETR)、Chl.a、类胡萝卜素(Car)、Chl.a/Chl.b和Car/Chl.a均显著降低;在快速光响应曲线中,当光照强度高于104 μmol/(m2·s)后,随光照强度的增加,附着水网藻的苦草叶片的rETR显著低于对照,光合作用能力下降;且其饱和光强为248μmol/(m2·s),显著低于对照叶片的685 μmol/(m2·s),表明水网藻附着苦草叶片后导致叶片光响应能力显著下降. 相似文献
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Ben Roudnew Justin R. Seymour Thomas C. Jeffries Trish J. Lavery Renee J. Smith James G. Mitchell 《Ground Water Monitoring & Remediation》2012,32(4):72-77
Bacteria and viruses are ubiquitous in subterranean aquatic habitats. Bacterial abundance is known to vary with depth in aquifers; however, whether viral abundance varies with depth is less well known. Here we use flow cytometry (FCM) to enumerate bacteria and virus‐like particles (VLP) from groundwater depth profiles. Groundwater samples were obtained from a set of nested piezometers from depths of 15, 30, 45, 60, 80, and 90 m and bacteria and VLP abundances were determined in purged aquifer water and unpurged water at each slot depth. Mean bacterial abundance (cells / mL) was not significantly different in unpurged water (3.2 × 105) compared to purged water (1.4 × 105); however, mean VLP abundance (particles / mL) was significantly greater in unpurged water (4.4 × 105) compared to purged water (2.3 × 105). Purged water was used to investigate the aquifer depth profile and bacterial and VLP abundances were observed to vary significantly between depths. The virus‐bacteria ratio was determined and was observed to steadily increase with depth. Overall, our data indicate the dynamic nature of bacterial and viral abundances in subsurface environments which should be considered when designing groundwater microbial sampling methodologies. 相似文献
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Bacterial abundances and activity, estimated by 4′,6-diamidino-2-phenylindole staining (DAPI) and the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT), were investigated in two oligotrophic artificial groundwater lakes and the surrounding aquifers. To evaluate the effect of lake water on groundwater downstream, samples were taken from wells at different distances from the lakes, and the total number of bacteria and the number of active bacteria in these samples were compared with samples collected upstream. In addition, sterilized sandy sediments were exposed in groundwater wells to measure the number and activity of bacteria attached to particles. At one of the study sites, where the lake sediments were disturbed by dredging, total bacterial abundance and the number of respiring bacteria in the groundwater aquifer was clearly influenced by the lake water. The average bacterial abundances decreased from 2.6 ± 1.9 × 105 cells ml−1 in the well closest to the lake (S2) to 2.9 ± 3.8 × 104 cells ml−1 in the most distant one (S4), which was equivalent to cell numbers in the upstream well. The number of respiring bacteria showed a similar tendency with 1.3 ± 2.7 × 104 active cells ml−1 in S2 and 1.9 ± 1.5 × 103 active cells ml−1 in S4. At the second study site, which was not influenced by dredging, bacteria in the downstream wells seemed not to be affected by the lake water. The number and activity of bacteria, which colonized exposed sediments, were not significantly different in the upstream and downstream wells, indicating a minor influence of lake water on this habitat. Our results suggest that gravel-pit lakes may influence the free living bacterial assemblages in nearshore groundwater systems, but do not visibly affect numbers and activity of bacteria attached to the surface of aquifer sediments. 相似文献
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Changes in octocoral communities and benthic cover along a water quality gradient in the reefs of Hong Kong 总被引：1，自引：0，他引：1
Cover of the main reef benthic groups, and abundances and taxonomic richness of octocorals were surveyed in the reefs of Hong Kong, and related to spatial and water quality gradients. Nutrient and particle concentrations are high throughout the area, with concentrations declining from the south towards the north-eastern region. Regression tree analyses showed that hard coral cover was most strongly related to water clarity, that macroalgal cover was highest in areas with high wave action and high water clarity, and that crustose coralline algae were negatively related to sedimentation. Octocoral communities (42 species in 23 genera) were dominated by zooxanthellae-free taxa; those few species with zooxanthellae were restricted to reefs with low wave action and high water clarity in the north-eastern region. The water quality gradient spans from conditions that are marginal for zooxanthellate octocorals while still supporting diverse scleractinian communities, towards an estuarine endpoint where zooxanthellate octocorals cease to exist and hard coral communities are reduced to a few resilient colonies. The data suggest that the types, abundances and richness of zooxanthellate octocorals, and the shift from zooxanthellate to azooxanthellate octocoral communities, may act as useful indicators of water clarity in regions where long-term water quality data are unavailable. 相似文献